hygiena SUS3000 SuperSnap High Sensitivity ATP Test Instruction Manual
- June 17, 2024
- Hygiena
Table of Contents
hygiena SUS3000 SuperSnap High Sensitivity ATP Test
Specifications:
- Product Name: SuperSnap Tests, SUS3000 and SUS3000X
- Intended Use: Detect invisible/trace amounts of residue
- Swabbing Area: Standard 10 x 10 cm (4 x 4 inch) for typical flat surface measurement
- Results Display: Within 15 seconds
Product Usage Instructions
Important Tips and Notes Before Starting the Test:
Ensure not to overload the swab with physical matter by swabbing visibly
dirty surfaces to avoid inaccurate results.
Test Procedure:
- Holding the swab tube firmly, twist and pull the top of the swab out of the tube.
- Thoroughly swab a standard 10 x 10 cm (4 x 4 inch) area for a typical flat surface measurement.
- Results will be displayed within 15 seconds.
Interpretation of Results:
Results are interpreted based on preset Pass and Fail RLU limits as per
industry standards. Refer to Table 1 for default RLU-Limit Settings.
FAQ
Q: How should I dispose of SuperSnap devices?
A: SuperSnap devices are made of 100% recyclable plastic and may be
discarded accordingly.
Q: What should I do if I suspect a defect in the product?
A: Promptly notify Hygiena within 5 days of discovery of any suspected
defect and return the product for replacement or refund. Contact Customer
Service for a Returned Goods Authorization Number.
Q: Where can I find technical support and resources?
A: For technical documents, refer to resources at
www.hygiena.com/documents. For technical
support, visit www.hygiena.com/support.
SuperSnap High Sensitivity ATP Test
For use with Hygiena ATP Monitoring Systems Product No. SUS3000 (100 tests,
with azide) and SUS3000X (100 tests, no azide)
Introduction
Description and Intended Use
SuperSnap tests are self-contained, highly sensitive devices that detect ATP
(adenosine triphosphate) when used with Hygiena luminometers. This system is
used for hygiene monitoring, HACCP-related monitoring of processing equipment,
cleaning validation, and other environmental monitoring, such as allergen
detection and prevention programs. SuperSnap devices are designed to detect
very low levels of organic residue with a tolerance for harsh samples that
could affect the bioluminescent reaction. As an allergen prevention tool,
SuperSnap tests can be used to verify the efficacy of cleaning procedures for
the removal of product residues that may contain potential allergenic
material. The enhanced sensitivity of SuperSnap tests allows the detection of
product residues down to 1 – 1,000 ppm depending on product matrices; this is
comparable to or better than the detection capabilities of allergen-specific
test methods. The system works by measuring ATP, the universal energy molecule
found in all animal, plant, bacterial, yeast and mold cells. Product residues
from organic matter left on surfaces contain ATP. Microbial contamination on a
surface also contains ATP but typically in smaller amounts. After proper
cleaning, all sources of ATP should be significantly reduced. When a sample is
collected and ATP is brought into contact with the unique liquid-stable
luciferase/luciferin reagent in the SuperSnap test device, light is emitted in
direct proportion to the amount of ATP present in the sample. The luminometer
measures generated light and reports results in relative light units (RLUs),
providing information on the level of contamination within seconds. The higher
the RLU number, the more ATP is present in the sample and the dirtier the
surface.
Note : SuperSnap tests are designed to detect invisible/trace amounts of residue. Overloading the swab with physical matter by swabbing a visibly dirty surface will inhibit the bioluminescent reaction and produce inaccurate results.
Important Tips and Notes Before Starting the Test
- Allow the SuperSnap device to equilibrate to room temperature (21 to 25 °C) before use.
- The swab tip is pre-moistened for maximum sample collection.
- Condensation may be visible on the inside of the swab tube; this is normal.
- Turn on the luminometer. If the luminometer has been programmed with test locations, select the appropriate location before running the test.
PRODUCT INSTRUCTION
Test Procedure
- Holding the swab tube firmly, twist and pull the top of the swab out of the tube.
- Thoroughly swab a standard 10 x 10 cm (4 x 4 inch) area for a typical flat surface. Important swabbing technique tips:
- For irregular surfaces, maintain a consistent swabbing technique during each test; swab a large enough area to collect a representative sample.
- Use aseptic techniques: Do not touch the swab or the inside of the sample device with your fingers.
- Swab in a crisscross pattern vertically, horizontally and diagonally in both directions.
- Rotate the swab while collecting the sample to maximize sample collection on the swab tip.
- Apply sufficient pressure to create flex in the swab shaft.
- Replace the swab back in the swab tube.
- To activate the device, hold the swab tube firmly and use your thumb and forefinger to break the Snap‑Valve by bending the bulb forward and backward. Squeeze the bulb twice to expel all the liquid down the swab shaft.
- Bathe the swab bud in the liquid by shaking for 5 – 10 seconds. Once activated, the sample must be read in the luminometer within 30 seconds.
- Holding the luminometer upright, insert the entire SuperSnap device into the Hygiena luminometer.
- Refer to the instrument manual for operating instructions. In brief:
- If using the EnSURE Touch luminometer, close the lid and press “Run Test” to initiate the measurement. Results will be displayed in 10 seconds.
- If using the EnSURE or SystemSURE Plus luminometer, close the lid and press “OK” to initiate the measurement. Results will be displayed in 15 seconds.
Additional Information
Interpretation of Results
Hygiena luminometers are preset with pass-and-fail RLU limits (Table 1) that
are based on industry standards and published study recommendations.
- SuperSnap tests are highly sensitive and produce more light per ATP molecule than other Hygiena tests. For example, SuperSnap tests are approximately 4 times more sensitive than UltraSnap tests (Product No. US2020). Using preset limits with
- SuperSnap tests will increase the standard of cleanliness; this is recommended when using SuperSnap tests as part of an allergen detection and prevention program.
- Hygiena recommends setting RLU thresholds according to the standards of your facility. For guidance, view the technical bulletin, Lower and Upper RLU Limits for ATP Monitoring Programs. To get the most out of your system, use SureTrend® software with
- Hygiena luminometers to track and trend testing performance over time.
- For technical documents, refer to the resources available at www.hygiena.com/documents. Contact your local sales representative or regional technical services team for additional support.
Calibration and Controls
It is advisable to run positive and negative controls according to Good
Laboratory Practice. Hygiena offers the following controls:
- ATP Positive Control Kit for ATP Test Devices (Product No. CK25)
- CalCheck LED Calibration Verification Device (Product No. CAL)
Storage and Shelf Life
-
Recommended storage is 2 to 8 °C (36 to 46 °F).
Before use, devices may be stored at room temperature (20 to 25 °C) for up to 4 weeks. -
Store SuperSnap devices out of direct sunlight.
-
Do not use past the expiration date on the label.
Disposal
SuperSnap devices are made of 100% recyclable plastic and may be discarded
accordingly.
Safety and Precautions
- Components of SuperSnap devices do not pose any health risk when used in accordance with standard laboratory practices and procedures of this insert.
- SuperSnap test devices are for one-time use. Do not reuse.
- For further safety instructions, refer to the Safety Data Sheet (SDS).
Hygiena Liability
Hygiena will not be liable to user or others for any loss or damage, whether
direct or indirect, incidental or consequential from use of these devices. If
this product is proven to be defective, Hygiena’s sole obligation will be to
replace the product or at its discretion, refund the purchase price. Promptly
notify Hygiena within 5 days of discovery of any suspected defect and return
the product to Hygiena; please contact Customer Service for a Returned Goods
Authorization Number.
Contact Information
For more information, visit
www.hygiena.com/contact. For technical
support, visit www.hygiena.com/support.
SuperSnap Tests, SUS3000 and SUS3000X INS-SUS3000-REVA
References
Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>