HACH LBOD101 Luminescent Optical Dissolved Oxygen User Manual

HACH LBOD101 Luminescent Optical Dissolved Oxygen

Product Information

oxygen
demand) samples
Additional Features| Temperature and absolute air pressure sensors for accurate
dissolved oxygen measurements stays on standard BOD bottles, stirs
the sample during measurements

Product Usage Instructions
Section 4: Preparation for use
Before using the Intellectual LBOD101 probe, ensure that it is properly prepared for use. Refer to the user manual for detailed instructions.

Section 5: Calibration
The Intellical LBOD101 probe requires calibration for accurate measurements. Follow the calibration notes and procedures provided in the user manual.

Calibration Notes
Read and understand the calibration notes before proceeding with the calibration process.

LBOD water-saturated air (100%) calibration procedure
Perform the LBOD water-saturated air (100%) calibration procedure as outlined in the user manual to calibrate the dissolved oxygen measurements.

Zero-point calibration procedure
Follow the zero-point calibration procedure to establish zero references for accurate dissolved oxygen measurements.

Section 6: Sample measurement
Use the Intellical LBOD101 probe to measure the dissolved oxygen concentration in BOD samples. Follow the sample measurement notes and procedure provided in the user manual.

Sample measurement notes
Read and understand the sample measurement notes before proceeding with the measurement process.

Sample measurement procedure
Follow the step-by-step sample measurement procedure provided in the user manual to obtain accurate dissolved oxygen readings.

Low-level measurements
If low-level measurements are required, refer to the user manual for specific instructions on performing such measurements.

Section 7: Maintenance
Proper maintenance of the Intellical LBOD101 probe ensures optimal performance and longevity. Follow the maintenance instructions provided in the user manual.

Clean the probe
Regularly clean the probe according to the instructions given in the user manual to prevent contamination and maintain accurate measurements.

Replace the sensor cap and iButton
If necessary, replace the sensor cap and iButton as described in the user manual to maintain accurate readings and functionality.

Replace the stirrer
If the stirrer becomes worn or damaged, follow the user manual instructions to replace it with a new one to ensure a proper sample stirring during measurements.

Storage
When not in use, store the Intellectual LBOD101 probe properly as instructed in the user manual to protect it from damage and maintain its performance.

Section 8: Troubleshooting
If any issues arise during the use of the Intellectual LBOD101 probe, refer to the troubleshooting section in the user manual for assistance in resolving common problems.

Section 9: Consumables
Refer to the user manual for information on consumables required for the proper functioning and maintenance of the Intellectual LBOD101 probe.

Section 1 Product Overview
The Intellical LBOD101 probe is a digital, luminescent dissolved oxygen sensor that measures the dissolved oxygen concentration of BOD (biochemical oxygen demand) samples. The probe has temperature and absolute air pressure sensors for accurate dissolved oxygen measurements. The probe stays on standard BOD bottles and stirs the sample during measurements. Refer to Figure 1.

Section 2 Specifications
Specifications are subject to change without notice.

system
Dissolved oxygen range| 0.05 to 20.0 mg/L (ppm); 1 to 200% saturation
Dissolved oxygen accuracy| ±0.05 mg/L for concentrations less than 10 mg/L O2

±0.1 mg/L for concentrations more than 10 mg/L O2

1. iButton is a registered trademark of Maxim Integrated Products, Inc.
2. An iButton is no longer supplied with the probe. It is not necessary for new probes to have an iButton, because the calibration data is installed on the probe at the factory.

Length: 215 mm (8.46 in.)

Cable length: 1.8 m (5.9 ft)

Cable connection| M12 digital output and connector
Warranty| 1 year on the probe. This warranty covers manufacturing defects, but not improper use or wear.
Certifications| CE, FCC/ISED

Section 3 Safety information

Intended use
The Intellical probes are intended for use by individuals who measure water quality parameters in the laboratory or in the field. The Intellical probes do not treat or alter water.

Use of hazard information

D A N G E R
Indicates a potentially or imminently hazardous situation that, if not avoided, will result in death or serious injury.

W A R N I N G
Indicates a potentially or imminently hazardous situation that, if not avoided, could result in death or serious injury.

C A U T I O N
Indicates a potentially hazardous situation that may result in minor or moderate injury.

NOT I C E
Indicates a situation which, if not avoided, may cause damage to the instrument. Information that requires special emphasis.

Precautionary labels
Read all labels and tags attached to the instrument. Personal injury or damage to the instrument could occur if not observed. A symbol on the instrument is referenced in the manual with a precautionary statement.

Electrical equipment marked with this symbol may not be disposed of in European domestic or public disposal systems. Return old or end-of-life equipment to the manufacturer for disposal at no charge to
the user.

Product hazards
C A U T I O N
Chemical exposure hazard. Obey laboratory safety procedures and wear all of the personal protective equipment appropriate to the chemicals that are handled. Refer to the current safety data sheets (MSDS/SDS) for safety protocols.

C A U T I O N
Chemical exposure hazard. Dispose of chemicals and wastes in accordance with local, regional, and national regulations.

Section 4 Preparation for use

1. Prepare the probe for calibration and measurement as follows. Do not touch the protective black layer on the LBOD sensor cap. Rinse the probe with deionized water. Blot dry with a lint-free cloth.
2. Make sure that the meter has the correct date and time settings. The service-life time stamp in the probe comes from the date and time settings in the meter.
   Note: Some meters automatically open the date and time settings when the meter starts for the first time or after battery replacement.

Connect the probe to the meter

Section 5 Calibration
The procedure that follows is applicable to meters that can connect to Intellical LBOD probes. Refer to the applicable meter documentation for meter operation and probe-specific settings.

Calibration notes
Read the notes that follow before calibration.

• Measure samples as soon as possible after collection.
• Use the single display mode for calibration when more than one probe is connected to the meter (if applicable).
• Calibrate the probes and verify the calibration regularly for best results. Use the meter to set calibration reminders.
• The calibration data is stored in the probe. When a calibrated probe is connected to a different meter with the same calibration options, a new calibration is not necessary.
• Air bubbles below the sensor when in solution can cause a slow response or error in the calibration. Make sure to remove air bubbles during calibration.
• The meter uses the slope value shown at the end of the calibration to monitor the condition of the sensor cap.

LBOD water-saturated air (100%) calibration procedure

1. Fill a BOD bottle approximately ¾ full with water.
2. Put a stopper on the bottle and shake for 30 seconds.
3. Rinse the probe with deionized water. Dry the probe with a lint-free cloth.
4. Carefully dry the LBOD sensor cap with a non-abrasive cloth.
5. Put the probe in the bottle. Wait approximately 10 minutes for the contents to adjust to ambient temperature.
6. Go to the calibrate menu. Select the probe, if applicable.
7. Read the dissolved oxygen value. The display shows 100% when the reading is stable.

Zero-point calibration procedure
A zero-point calibration can increase the measurement accuracy of samples that have less than 1 mg/L O2. A zero-point calibration is not recommended for samples that have more than 1 mg/L O2. Set the calibration mode to “100% with 0”. The meter starts the calibration with the 100% measurement and then continues to the zero-point measurement.

1.  Fill a BOD bottle with deionized water.
2. Add 300 mg of sodium sulfite to the bottle.
3. Add 2 mL of cobalt chloride solution to the bottle.
4. Put a stopper on the bottle and fully mix for 30 seconds.
5. Remove the stopper. Put the probe in the bottle.
6. Tap the probe to remove air bubbles.
7. Wait approximately 10 minutes for the contents to adjust to ambient temperature.
8. Start the stirrer.
9. Read the dissolved oxygen value. Wait for the measurement to become stable.
10. Save the calibration.
11. Rinse the probe with deionized water. Dry the probe with a lint-free cloth.
12. Fill a BOD bottle with deionized water. Put the probe in the bottle and stir for 10 minutes to fully remove the sulfite from the probe.

Section 6 Sample measurement
The procedure that follows is applicable to meters that can connect to Intellical LBOD probes. Refer to the applicable meter documentation for meter operation and probe-specific settings.

Sample measurement notes
Read the notes that follow before sample measurements.

• Salinity changes the solubility of oxygen in the water. Measure the sample salinity and enter the value in the probe settings of the meter.
• High concentrations (more than 1 molar) of acids or bases will decrease the service life of the LBOD sensor cap.
• Rinse the probe with deionized water and dry it with a lint-free cloth between measurements to prevent contamination.
• If complete traceability is necessary, enter a sample ID and operator ID before measurement. Refer to the meter manual for instructions.
• The meter automatically saves the measurement data when the user manually reads each data point and when the meter is set to read at regular intervals. The user must manually save each data point when the meter is set to read continuously.
• Air bubbles below the sensor can cause a slow response or error in the measurement. Make sure to remove air bubbles before and during measurements.

Sample measurement procedure

1. Rinse the probe with deionized water. Dry the probe with a lint-free cloth.
2. Put the probe in the BOD sample.
3. Tap the probe to remove air bubbles.
4. Start the stirrer. Read the dissolved oxygen value of the sample.
5. The display shows the dissolved oxygen value when the reading is stable.

Low-level measurements

• If samples are expected to have less than 1 mg/L O2, calibrate the probe with a zero-oxygen solution.
• A zero-point calibration is not recommended for samples that have more than 1 mg/L O2. Use the guidelines and steps that follow only when the sample is expected to have less than 1 mg/L O2.
• Calibrate the probe with a zero-oxygen solution. Use the “100% with 0” calibration option in the meter. Measure the 100% value first, then measure the zero-oxygen solution. Refer to the Zero-point calibration procedure on page 6.
• Measure the salinity of the sample and enter the salinity correction factor in the probe settings.
• Wait approximately 10 minutes for the measurement contents to adjust to ambient temperature, then measure the sample. Measure the sample again to make sure the result is the same.
• Clean the probe regularly. Refer to Clean the Probe on page 8.

Section 7 Maintenance
Clean the probe
NOT I C E
The LBOD sensor cap has a protective black layer to increase the life of the LBOD sensor. Do not rub the black layer to clean the LBOD sensor cap. Do not use alcohol or other organic solvents to clean the LBOD sensor cap. Keep the LBOD sensor cap clean for the best results. Use only water and neutral detergents to clean the probe. Put the probe in a neutral cleaning solution and stir the solution. Do not rub or remove the black layer on the LBOD sensor cap. Rinse the probe with deionized water. Blot dry with a lint-free cloth.

Replace the sensor cap and iButton
NOT I C E
Do not use sharp metal tools to remove the LBOD sensor cap. Replace the LBOD sensor cap after 365 days, or more frequently if the cap becomes damaged or dirty. The LBOD sensor cap and iButton operate together and must be replaced at the same time. Refer to the instructions that are supplied with the LBOD sensor cap replacement kit. If water is between the LBOD sensor cap and the probe lens, or if the probe lens looks dirty, rinse with dilute isopropyl alcohol (10% or less) or deionized water and blot dry with a non-abrasive cloth. Do not wipe the probe lens or use abrasive cleaners.

Replace the stirrer
Refer to Figure 2 to replace the stirrer assembly. Figure 2 Replace the stirrer

Storage
Keep the probe in a BOD bottle with some water (fill to ¼ minimum) when not in use.

Section 8 Troubleshooting

Decreased probe performance causes slow stabilization and prevents accurate calibrations or measurements.| The sensor cap is loose or damaged.| Tighten or replace the sensor cap. Always replace the sensor cap and the iButton at the same time. Refer to Replace the sensor cap and iButton on page 9.
Water is between the sensor cap and the probe lens.| Remove the sensor cap and dry the probe lens. Refer to Replace the sensor cap and iButton on page 9.
The sensor cap is not sufficiently conditioned.| Keep the probe in the BOD bottle with some water for more time, then try the calibration again.
The temperature or pressure sensor does not operate correctly.| Compare the temperature and pressure readings of the probe with external measurements. The pressure sensor reads absolute pressure, which is not adjusted to sea level. If the measurements are not correct, contact technical support.
The lot code on the iButton is not the same as the lot code on the sensor cap.| Replace the sensor cap and iButton.
The stirrer does not operate correctly.| The stirrer does not operate.| The stirrer uses the power from the meter to operate. Make sure to start the meter, then start the stirrer.
The stirrer turns only when the probe is not in a BOD bottle.| Remove and install the stirrer assembly. Refer to Replace the stirrer on page 10.
The stirrer makes a lot of noise.| Remove and install the stirrer assembly. Refer to Replace the stirrer on page 10.
Sample properties cause slow stabilization or inaccurate measurements.| The measurement was not adjusted for salinity in the sample.| Measure the salinity of the sample and enter the value as a salinity correction factor in the meter.
Procedure problem causes slow stabilization and prevents accurate calibrations or measurements.| Air bubbles are around or below the probe tip.| Carefully tap or shake the probe to remove air bubbles.
The contents of the BOD bottle did not fully adjust to ambient temperature.| Wait more time for the contents of the BOD bottle to adjust to ambient temperature.

Section 9 Consumables
Note: Product and Article numbers may vary for some selling regions. Contact the appropriate distributor or refer to the company website for contact information.

Section 9 Consumables (continued)

HACH COMPANY World Headquarters
P.O. Box 389, Loveland, CO 80539-0389 U.S.A.
Tel. 970-669-3050 800-227-4224 (U.S.A. only)
Fax 970-669-2932
[email protected] www.hach.com

References

• Geräte und Reagenzien für die Wasserqualitätsanalyse | Hach
• Hach | Hach

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