Labnet C2500-R Prism R Refrigerated Microcentrifuge Instruction Manual

Labnet C2500-R Prism R Refrigerated Microcentrifuge

Introduction

The Labnet Prism™ R refrigerated microcentrifuge is a small benchtop centrifuge designed for both research and clinical laboratories. The motor is brushless and requires no routine maintenance. This microcentrifuge is supplied with a 24 x 1.5/2.0 mL rotor for microsample tubes. Optional StripSpin adapter is available for 0.2 mL tubes and strips. This reaches speeds of up to 13,500 rpm/17,135 x g. The temperature range is from -10°C to 40°C. Powerful refrigeration system, maintain 4°C at maximum speed.

Safety Information

Before using the Labnet Prism R refrigerated microcentrifuge for the first time, please read this entire manual carefully. If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

**** This symbol indicates a potential risk and alerts you to proceed with caution.

Operation Safety Precautions

• Never use the centrifuge in any manner not specified in these instructions.

• Never operate the centrifuge without a rotor properly attached to the shaft.

• Always use a wrench to tighten the rotor nut. Never tighten rotor nut by hand only.

• Never fill tubes while they are in the rotor. Liquid spillage may harm unit.

• Never put hands in the rotor area unless the rotor is completely stopped.

• Never move the centrifuge while the rotor is spinning.

• Never use solvents or flammables near this or other electrical equipment.

• Never centrifuge flammable, explosive, or corrosive materials.

• Never centrifuge hazardous materials outside of a hood or a proper containment facility.

• Always load the rotor symmetrically. Each tube should be counter-balanced by another tube of the same type and weight.

• Always locate the centrifuge within easy access to an electrical outlet.

• Always use only microcentrifuge tubes made from plastic and designed to withstand centrifugal forces of at least 17,135 x g.

• Always use a wrench to tighten the rotor nut.
  Do not operate the centrifuge if any of the following conditions exist:

• The centrifuge has not been installed properly.

• The centrifuge is partially dismantled.

• Service has been attempted by unauthorized or unqualified personnel.

• The rotor has not been installed securely on the motor shaft.

• Rotors and accessories outside of the standard range are not to be used without permission from the manufacturer.
  Exception: Microcentrifuge tubes made of plastic, normally available in the laboratory.

• The centrifuge is located in an explosive atmosphere.

• Materials to be centrifuged are combustible and/or explosive.

• Materials to be centrifuged are chemically reactive.

• The rotor load is not properly balanced.

• The rotor nut was not tightened with a wrench.

Specifications

230V ±10%, 50/60 Hz, 3.15A
Operating Conditions| 5°C to 30°C, ≤80% RH
Noise at Maximum Speed| 56 dB
Acceleration Time| <17 seconds
Deceleration Time| <18 seconds
Temperature Range| -10°C to 40°C
Rotor Lid| Easy snap-on type

The Labnet Prism™ R refrigerated microcentrifuge is designed to be safe at least when operated under the following conditions:

• Indoor use only
• Altitude up to 2,000 meters
• Pollution Degree 2

Package Contents

• Labnet Prism R refrigerated microcentrifuge
• Rotor with lid, 24 x 1.5/2 mL
• Rotor removal tool
• Electric fuses (7A and 3.15A)
• Power cord
• Instruction manual

Installation

The centrifuge should be installed on a rigid, even surface such as a stable laboratory bench, countertop, etc. To guarantee sufficient ventilation, ensure that the centrifuge has at least 12 inches (30 cm) of free space on all sides, including the rear. The centrifuge should not be located in areas subject to excessive heat such as in direct sunlight or near radiators or the exhaust of a compressor, as a buildup of heat may occur within the chamber.

Before operating the centrifuge, check that the power source (electrical outlet on the wall) corresponds to that on the manufacturer’s rating label, then connect the power cord to the centrifuge and the power source.

Installation of Rotors and Rotor Maintenance

Rotor Maintenance

The rotor should be cleaned thoroughly after each use. NOTE: Thorough cleaning must be performed when spinning samples containing phenol or phenol chloroform. Periodically inspect the rotor for dents, dings, scratches, discoloration, and cracks. If any damage to the rotor is found, discontinue use of the rotor immediately and replace.

Removing and Installing the Rotor

The Labnet Prism™ R refrigerated microcentrifuge comes complete with a standard 24-place rotor installed. To remove the rotor for cleaning, using the rotor wrench remove the rotor securing screw from the motor shaft by turning the screw counterclockwise. Lift the rotor directly upward in a straight vertical motion.

• Figure 1. Loading the rotor to achieve balance.
  

To replace the rotor, first make sure the motor shaft and rotor mounting hole are clean. Place the rotor on the motor shaft. Reinstall the rotor securing screw on the motor shaft by turning it clockwise. Hold the rotor with one hand and tighten the rotor securing screw, using the rotor wrench.

Loading the Rotor

Tubes to be loaded should be filled equally by eye. The difference in the weight between the tubes should not exceed 0.1 gram. Tubes should always be loaded so there is equal spacing between all tubes. One or two additional loaded tubes may need to be added to achieve this. See Figure 1 for a typical balancing scheme.

Overloading Rotors

The maximum load of the rotor and maximum speed has been established by the manufacturer. Do not attempt to exceed these values. The maximum speed of the rotor has been established for liquids having a homogeneous density of 1.2 g/mL or less. In order to centrifuge liquids with a higher density it is necessary to reduce the speed. NOTE: Failure to reduce the speed may result in damage to the rotor and centrifuge. The revised maximum speed can be calculated with the following formula:

Example: Where the density of the liquid is 1.7, the new maximum speed would be calculated as follows:

If in doubt concerning maximum speeds, please contact the manufacturer for assistance.

Overview

Keypad Function

• Start/Stop and Value Adjustment Knob (Start/Stop):
  • Press down to Start/Stop a run
  • Rotate to increase/decrease the values of time, temperature, speed or g-force
• Time: Adjusts run time setting
• RPM: Adjusts speed setting
• RCF: Adjusts RCF setting
• Temp: Adjusts temperature setting
• Lid: Opens the lid
• Quick: Quick-Spin centrifugation

Audible Signals

The audible signals are beneficial when you are familiarizing yourself with the operating procedure of the Labnet Prism™ R refrigerated microcentrifuge. They can also be disabled if desired by pressing the Time button six times. The audible signal function can be resumed by pressing the Time button six times again.

Definition of the audible sounds:

• 2 beeps: Power on machine
• 3 beeps: End of a run

Operation

CAUTION: Never attempt to operate the centrifuge with rotors or adapters that show signs of corrosion or mechanical damage. Never centrifuge strongly corrosive materials that may damage the rotors, accessories, or bowl of the unit.

Attaching Rotor Lid

After the rotor has been properly secured and loaded, attach the rotor lid to the rotor. Always use the rotor lid for safety and to allow the rotor to reach proper speed. Make sure the rotor lid snaps securely into place, by pressing down on center catch.

Opening and Closing the Lid

The lid can be opened only when the centrifuge is powered on. Press the Lid button to open the lid. The lid will be locked automatically when the user presses down the front edge of the lid.

NOTE: Do not slam the lid.

Emergency Lid Release

When the lid can’t be opened by pressing the Lid button, user can open the lid manually.

CAUTION: Do not attempt to open the lid of any centrifuge until the rotor has come to a complete stop.

1. Disconnect the power cord from the wall socket.
2. Use a flat-head screw driver or other tool to remove the Emergency Lid Release Plug, located on the right-hand side of the unit.
3. Pull the wire (attached to the plug) to open the lid lock manually.

Setting the Time

Operating time can be selected from 0.5 min. to 99 min. or continuous. Press the Time button to change the time setting. The time on the display will flash. Turn the Start/Stop knob to increase or decrease the value. Press the Time button again to confirm the selection or press Start/Stop knob to store and run the selection. The flashing of time value will stop and the time value will be stored automatically if no button is pressed after 6 seconds of the adjustment.

Operating time can be set as continuous by turning the Start/Stop knob clockwise or counterclockwise to make the time to display “- -“. When the time is set as continuous, the centrifuge will continue to run without a stop. Press Start/Stop knob to end the continuous operation.

Setting the Speed (RPM)

The speed (RPM) can be selected from 500 to 13,500 rpm. Press the RPM button to change the speed setting. The speed (RPM) on the display will flash. Turn the Start/Stop knob to increase or decrease the value. Press the RPM button again to confirm the selection or press the Start/Stop knob to store and run the selection. The flashing of the RPM value will stop, and the RPM value will be stored automatically if no button is pressed after 6 seconds of the adjustment.

Setting the Centrifugal Force (RCF)

The speed can be selected from 20 to 17,135 x g. Press the RCF button to change the RCF setting. The RCF on the display will flash. Turn the Start/Stop knob to increase or decrease the value. Press the RCF button again to confirm the selection or press the Start/Stop knob to store and run the selection. The flashing of the RCF value will stop, and the RCF value will be stored automatically if no button is pressed after 6 seconds of the adjustment.

Setting the Temperature (Temp)

The temperature can be selected from -10°C to 40°C. Press the Temp button to change the temperature setting. The temperature on the display will flash. Turn the Start/Stop knob to increase or decrease the value. Press the Temp button again to confirm the selection or press Start/Stop knob to store and run the selection. The flashing of the Temp value will stop, and the value will be stored automatically if no button is pressed after 6 seconds of the adjustment.

Cooling

The temperature can be set from -10°C to 40°C. The set temperature can also be changed during the run.

Pre-cooling

Pre-cooling starts a temperature-control run at 6,000 rpm. It allows the rotor to be cooled down to the new, pre-set temperature quickly. Cooling down from room temperature to 4°C takes approximately 8 min. (max. 16 minutes). To activate the Pre-cooling function, press and hold the Temp button, then press the Start/Stop knob. Both RPM and Temp icons will flash during Pre-cooling. The Pre-cooling can be stopped by pressing the Start/Stop knob. The Pre- cooling will function only when the set temperature is lower than the ambient temperature.

Standby Cooling

When the centrifuge is powered on, the refrigeration system will cool down the chamber to the pre-set temperature before or after the run if the chamber temperature is above the pre-set temperature.

NOTES:

• To extend the life cycle of the compressor, always remember to inactivate the Standby Cooling (by increasing the set temperature above room temperature) or power off the centrifuge when it is not in use.
• When the lid is open, the Standby Cooling will stop after 3 minutes.

Starting and Stopping a Run

• To start a run, press the Start/Stop knob.
• When the pre-set time expires, the centrifuge will stop automatically.
• To stop the centrifuge prior to the expiration of set time, press the Start/Stop knob again.

When the time is set as continuous ( – – ), the centrifuge will run continuously. To stop the continuous run, press the Start/Stop knob. By pressing and holding the Quick button, the centrifuge will continue a short- run of set speed. The short-run will stop if the Quick button is released.

NOTE: For safety reasons, the Start/Stop knob will be inactivated when the lid is open. A symbol “Lid“ will be flashing until the lid is closed.

Quick-Spin Operation

The centrifuge can be operated for a short run by pressing and holding the Quick button. The centrifuge will continue to run as long as the Quick button is depressed and the time, in seconds, will count up on the time display. The Quick-Spin speed and temperature is based on the last run settings remaining in the memory. The icon RPM will flash during the Quick-Spin operation.

Changing the Settings During a Run

Users can change the settings while the rotor is running. Through a one-time pressing of any function buttons (Temp, Time, RPM, RCF), the current value will switch into the pre-set value mode.

The current value flashes, and the new values can be input. Once the display switches into the current value mode after completion of the entered values, the new values are activated. The new values are stored and executed by pressing any function button again or by waiting 6 seconds.

Service and Maintenance

Centrifuge Service

The brushless motor in this microcentrifuge requires no routine maintenance. Any required service should be performed by authorized, qualified personnel only. Repairs performed by unauthorized personnel may void the warranty.

Cleaning

Always keep the centrifuge housing, rotor chamber, rotor and rotor accessories clean. All parts should be wiped down periodically with a soft cloth. For more thorough cleaning, use a neutral cleaning agent (pH between 6 and 8) applied with a soft cloth. Excessive amounts of liquid should be avoided. Liquid should not come into contact with the motor. After cleaning, ensure that all parts are dried thoroughly by hand or in a warm air cabinet (maximum temperature 50°C).

The rotor should be cleaned after each use. When spinning samples containing phenol or phenol chloroform, the rotor should be cleaned immediately after use.

Should a spill of infectious materials occur within the rotor or chamber, the unit should be disinfected. This should be performed by qualified personnel with proper protective equipment.

Replacing Fuses

Check the fuse when it is recommended (see Troubleshooting Guide section). Disconnect the power cord from the wall socket before checking the fuses. The fuse holder is located in the power inlet on the rear of the unit. Open the fuse holder drawer by inserting a small screwdriver under the tab and prying it open. Remove the innermost (operative) fuse from its retaining tabs, and replace the fuse if necessary. A spare fuse is located in the outermost chamber of the fuse drawer. Replace only with a fuse of exactly the same value as the original. See Section 3.0 for information on fuse type.

Troubleshooting Guide

• Check if power is being supplied to the outlet.
• Check if the power cord is plugged into both the wall outlet and the back of the centrifuge.
• Check if the power cord is damaged.

Blown fuse| Check the fuse and replace if necessary.
Lid lock will not release| Defective lid lock| Open manually, and have the unit serviced.
No power from the PC board| Call for service.
Lid lock is jammed| Call for service.
Centrifuge is not receiving power| See “Centrifuge will not start”.
Centrifuge cannot be started, although power is On| Lid is not closed correctly| Close the lid correctly.
No speed or time has been selected| Set the speed and/or time.
bAL (Error Message) indicates imbalance| Tubes are not inserted symmetrically in the rotor holes| Load the tubes symmetrically (see Section 6.3: Loading the Rotor).
Sample liquid in the tubes is not balanced| Make sure the same volume of liquid is in each tube.
Defective or improperly adjusted balance sensor| Call for service.
Rotor is stuck when a run starts.| Call for service.
Lid (Error Message) lid not closed| Lid is not closed completely| Close the lid.
Lid lock or sensor is defective| Call for service.
Other Error Messages Er on Display| –|

• Press the time or speed button to clear the error.
• Call for service.

Should you have a question about the operation of the Labnet Prism™ R refrigerated microcentrifuge or if service is required, contact Corning Customer Service. Do not send in a unit for service without first calling to obtain a repair authorization number. Should the unit require return for service, it should be properly packed to avoid damage. Any damage resulting from improper packaging shall be the responsibility of the user.

Determination of G-values

The centrifuging radius of the 1.5/2.0 mL rotor is 8.23 cm. The Labnet Prism™ R refrigerated microcentrifuge has an automatic g-force conversion program, so g-values are automatically calculated and can be displayed on the control panel, for this centrifuging radius. If adapters or smaller tubes are used, the centrifuging radius changes as does the g-force. The chart below can be used to manually determine g-values for any centrifuging radius.

To calculate the RCF value at any point along the tube or bottle, measure the radius (in mm), from the center of the centrifuge spindle to the particular point. Draw a line from the radius value on the right-hand column to the appropriate centrifuge speed on the left hand column. The RCF value is the point where the line crosses the center column. The nomogram is based on the formula:

RCF = (11/17 x 10-7) RN2

where:

R = Radius in mm from centrifuge spindle to point in tube bottom
N = Speed of spindle in RPM

Limited Warranty

Corning Incorporated (Corning) warrants that this product will be free from defects in material and workmanship for a period of two (2) years from date of purchase. CORNING DISCLAIMS ALL OTHER WARRANTIES WHETHER EXPRESSED OR IMPLIED, INCLUDING ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR OF FITNESS FOR A PARTICULAR PURPOSE. Corning’s sole obligation shall be to repair or replace, at its option, any product or part thereof that proves defective in material or workmanship within the warranty period, provided the purchaser notifies Corning of any such defect. Corning is not liable for any incidental or consequential damages, commercial loss or any other damages from the use of this product.

This warranty is valid only if the product is used for its intended purpose and within the guidelines specified in the supplied instruction manual. This warranty does not cover damage caused by accident, neglect, misuse, improper service, natural forces or other causes not arising from defects in original material or workmanship. This warranty does not cover motor brushes, fuses, light bulbs, batteries or damage to paint or finish. Claims for transit damage should be filed with the transportation carrier.

In the event this product fails within the specified period of time because of a defect in material or workmanship, contact Corning Customer Service at: USA/Canada 1.800.492.1110, outside the U.S. +1.978.442.2200, visit www.corning.com/lifesciences, or contact your local support office.

Corning’s Customer Service team will help arrange local service where available or coordinate a return authorization number and shipping instructions. Products received without proper authorization will be returned. All items returned for service should be sent postage prepaid in the original packaging or other suitable carton, padded to avoid damage. Corning will not be responsible for damage incurred by improper packaging. Corning may elect for onsite service for larger equipment.

Some states do not allow limitation on the length of implied warranties or the exclusion or limitation of incidental or consequential damages. This warranty gives you specific legal rights. You may have other rights which vary from state to state.

No individual may accept for, or on behalf of Corning, any other obligation of liability, or extend the period of this warranty.

Equipment Disposal

According to Directive 2012/19/EU of the European Parliament and of the Council of 4 July 2012 on waste electrical and electronic equipment (WEEE), this product is marked with the crossed-out wheeled bin and must not be disposed of with domestic waste.

Consequently, the buyer shall follow the instructions for reuse and recycling of waste electronic and electrical equipment (WEEE) provided with the products and available at www.corning.com/weee.

To request certificates, please contact us at www.labnetlink.com.

Warranty/Disclaimer: Unless otherwise specified, all products are for research use or general laboratory use only. Not intended for use in diagnostic or therapeutic procedures. Not for use in humans. These products are not intended to mitigate the presence of microorganisms on surfaces or in the environment, where such organisms can be deleterious to humans or the environment. Corning Life Sciences makes no claims regarding the performance of these products for clinical or diagnostic applications. For a listing of US medical devices, regulatory classifications or specific information on claims, visit www.corning.com/resources.

Customer Support

Corning Incorporated
Life Sciences
www.corning.com/lifesciences

NORTH AMERICA
t 800.492.1110
t 978.442.2200
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Australia/New Zealand
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Brazil
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t (52-81) 8158-8400

For a listing of trademarks, visit www.corning.com/clstrademarks. All other trademarks are the property of their respective owners.
© 2022 Corning Incorporated. All rights reserved. 11/22 CLSLN-AN-1033DOC REV1

References

• Legal Notices | Corning.com
• Labnet |

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