GOLD STANDARD DIAGNOSTICS 544052 1 mL Streptavidin Magnetic Beads Owner’s Manual

: September 25, 2024
: GOLD STANDARD DIAGNOSTICS

Table of Contents

GOLD STANDARD DIAGNOSTICS 544052 1 mL Streptavidin Magnetic Beads
Specifications
FAQs
General Description
Storage Buffer
Storage and Stability
Warning and Precautions
Characteristics
WARRANTY
CONTACT INFORMATION
References
Read User Manual Online (PDF format)
Download This Manual (PDF format)

GOLD STANDARD DIAGNOSTICS 544052 1 mL Streptavidin Magnetic Beads

Specifications

Product No.: 544052 (1 mL), 544050 (2 mL), 544051 (5 mL)
Storage: Phosphate buffered saline with detergent and
preservative

FAQs

Q: What is the warranty coverage for the product?

A: Gold Standard Diagnostics offers a limited warranty against defects and workmanship when used according to instructions until the product’s printed expiration date.

Q: How can I contact Gold Standard Diagnostics for ordering or technical support?

A: You can contact Gold Standard Diagnostics at 795 Horsham Road Horsham, PA 19044, or visit their website at www.abraxiskits.com. For ordering, email info.abraxis@us.goldstandarddiagnostics.com, and for technical support, email support.abraxis@us.goldstandarddiagnostics.com. You can also reach them by phone at 215-357-3911 or by fax at 215-357-5232.

General Description

The Gold Standard Diagnostics’ superparamagnetic nanoparticles are coupled with a biomolecule, such as Streptavidin, and are utilized in the magnetic separation and isolation of biotin-labeled components. The magnetic beads have a large surface area with high capture efficiencies.

Storage Buffer

The reagent is stored in phosphate-buffered saline with detergent and preservative.

Storage and Stability

The Streptavidin Magnetic Beads should be stored in the refrigerator (2-8°C). The reagent must be allowed to reach room temperature (20-25°C) before use and may be used until the last day of the month as indicated by the expiration date on the vial. Do not freeze, dry, or centrifuge the magnetic beads as they may result in loss of binding activity and aggregation.

Test Principle

Streptavidin magnetic beads are incubated with the biotin-labeled solution and then separated by magnets. After the unbound particulates are washed from the beads, the bound biotin is eluted from the beads using the elution buffer. The beads are then magnetically separated from the eluted solution, which is removed manually

Warning and Precautions

Do not freeze the reagent.
Prior to use, ensure that the product has not expired by verifying that the date of use is prior to the expiration date on the label.
Ensure that reagent bottle caps are tight after each use to prevent drying of reagents.
Mistakes in handling the test can also cause errors. Possible sources for such errors can be inadequate storage conditions of the test kit (or reagents), incorrect pipetting sequence or inaccurate volumes of the reagents, too long or too short incubation times, and/or short magnetic separation times.
The reagent contains 0.05% sodium azide as a preservative. Sodium azide may react with lead or copper plumbing to produce metal azides which might cause an explosion. To prevent azide accumulation in plumbing, flush with copious amounts of water immediately after disposal.

Characteristics

Bead mean diameter: ~0.5 µm
Bead concentration: 5 mg/mL
Binding capacity: ≥60 µg Biotin-IgG /mg of beads

A. Materials Provided

Streptavidin magnetic beads, 5 mg/mL

B. Additional Materials (not provided with the kit)

Binding/Wash Buffer: TBS – 0.05% Tween 20 detergent
Elution Buffer: 0.1 M Glycine pH 2.0, 5 mL
Neutralization Buffer: 1M Tris pH 8.0, 1 mL
Micro-pipettes with disposable plastic tips (10-200 and 200-1000 µL)
1.5 mL or 2.0 mL Eppendorf or microcentrifuge vials
Timer
Rotator
Distilled or deionized water
Vortex mixer
Solo or Multi-6 Microcentrifuge Separator (PN 472270; PN 472260)

Procedures

Add 100 µL (0.5 mg) of beads to 1 mL of binding buffer in each tube to wash the beads.
Magnetically separate using a magnetic separator for 2 minutes or when the supernatant is clear.
Remove the supernatant and wash once more by adding 1 mL of binding buffer.
Repeat step 2 and remove the supernatant.
Resuspend beads by adding 450 µL of binding buffer.
Add 50 µL of serum or cell culture supernatant to the particles.
- Note : Sample volume can be modified according to user preference. If the sample supernatant volume is < 50 µL, dilute it to a final volume of 500 µL with Binding/Wash Buffer.
Gently mix using vortex or rotator for 30 minutes.
Magnetically separate using a magnetic separator for 2 minutes or when the supernatant is clear.
Remove and discard supernatant.
Add 500 µL Binding/Wash buffer to wash the beads and remove unbound proteins.
Repeat steps 8 and 9 once more. Remove supernatant.
Add 100 µL of elution buffer to beads and mix well.
Incubate at room temperature for 10 minutes with occasional gentle mixing or vortex.
Separate for 2 minutes and remove the eluent to a new tube containing 15 µL of neutralization buffer.

AbraMag® Magnetic Beads are superparamagnetic, non-aggregating iron oxide particles (or ‘microspheres’) for sample prep, or for capturing/purifying targets such as proteins, antibodies, DNA/RNA, exosomes, and E. coli. AbraMag’s design enables faster binding kinetics, with high sensitivity & selectivity, in both manual and automated biomedical and research applications

Superior yield, purity, quality, and value over the leading competitors.

Multiple Advantages – Over conventional methods (columns, centrifugation).
Superior Performance – We’ve designed them to match or outperform the competition.
Superior Capacity and Yield – High binding capacity for rapid and efficient target purification.
Superior Purity – Stable, pre-blocked particles provide clean purification even from complex samples.
Customizable – Custom beads and coupling services are available

WARRANTY

General Limited Warranty : Gold Standard Diagnostics warrants the products manufactured by the Company, against defects and workmanship when used in accordance with the applicable instructions for a period not to extend beyond the product’s printed expiration date. Gold Standard Diagnostics makes no other warranty, expressed or implied. There is no warranty of merchantability or fitness for a particular purpose.