GILSON Pipetman G Pipette User Guide

Quick Guide

Introduction

PIPETMAN® G is a fully adjustable air-displacement, pipette that is used with disposable tips. To meet pipette user’s needs, PIPETMAN G relies on the legendary accuracy, precision, and reliability of PIPETMAN, with pipetting and purge forces dramatically decreased for hours of comfortable pipetting.
Eight single channel models cover a volume range from 0.2 µL to 10 mL.
Eight multichannel models cover a volume range from 1 µL to 300 µL.

NOTICE PIPETMAN G is identified by a visual blue ring.

Parts Checklist
Single models

• PIPETMAN G
• PIPETMAN G Quick Guide
• Safety bag
• Dual-position adapter (for P2G and P10G models only, refer to Fitting the Tips on page 6)
• Certificate of Conformity (including barcode sticker)

Multichannel models

• PIPETMAN G
• PIPETMAN G Quick Guide
• Safety bag
• Ejector spacers for D10 tips (for Px10G models only, refer to Ejector Spacers for PIPETMAN G 10 µL Multichannel Models on page 7)
• Certificate of Conformity (including barcode sticker)

Good Laboratory Practice (GLP) Compliance
The serial number is engraved on the body of the pipette. It provides unique identification of your pipette and the date of manufacture. For more details, refer to PIPETMAN G User’s Guide LT801122.
Ex: AA10369
The barcode on the box and the Certificate of Conformity provide traceability of your pipette.

Description

Figure 2
PIPETMAN® G multichannel and single models

Specifications

PIPETMAN G is a high quality pipette that offers excellent accuracy and precision. The data provided in the Maximum Permissible Errors table on page 4 were obtained using PIPETMAN® DIAMOND Tips.
These values are guaranteed only when genuine PIPETMAN DIAMOND Tips are used.

NOTICE
With a precise pipetting technique the P2G model may be used to aspirate volumes as low as 0.1 µL and the P10G model as low as 0.5 µL.

Maximum Permissible Errors
The data provided in the following table conform to the ISO 8655-2 standard.

MAXIMUM PERMISSIBLE ERRORS

VOLUME (µL)

| GILSON| ISO 8655
SYSTEMATIC ERROR (µL)| RANDOM ERROR (µL)| SYSTEMATIC ERROR (µL)| RANDOM ERROR (µL

PIPETMAN G SINGLE CHANNEL

P2G (P/N F144054M) WITH D10 OR DL10 TIPS

0.2| ± 0.024| ≤0.012| ± 0.08| ≤0.04
0.5| ± 0.025| ≤0.012| ± 0.08| ≤ 0.04
1| ± 0.025| ≤0.012| ± 0.08| ≤0.04
2| ± 0.030| ≤0.014| ± 0.08| ≤0.04
P10G (P/N F144055M) WITH D10 OR DL10 TIPS
1| ± 0.025| ≤0.012| ± 0.12| ≤ 0.08
5| ± 0.075| ≤ 0.030| ± 0.12| ≤ 0.08
10| ± 0.100| ≤0.040| ± 0.12| ≤ 0.08
P20G (P/N F144056M) W ITH D200 TIPS
2| ± 0.10| ≤0.030| ± 0.2| ≤ 0.1
10| ± 0.10| ≤0.050| ± 0.2| ≤ 0.1
20| ± 0.20| ≤0.060| ± 0.2| ≤0.1
P100G (P/N F144057M) WITH D200 TIPS
10| ± 0.35| ≤ 0.10| ± 0.8| ≤ 0.3
50| ± 0.40| ≤0.12| ± 0.8| ≤ 0.3
100| ± 0.80| ≤0.15| ± 0.8| ≤ 0.3
P200G (P/N F144058M) WITH D200 TIPS
20| ± 0.50| ≤ 0.20| ± 1.6| ≤0.6
100| ± 0.80| ≤0.25| ± 1.6| ≤ 0.6
200| ± 1.60| ≤0.30| ± 1.6| ≤0.6
P1000G (P/N F144059M) WITH D1000 TIPS
100| ± 3.0| ≤ 0.6| ± 8.0| ≤ 3.0
500| ± 4.0| ≤1.0| ± 8.0| ≤3.0
1000| ± 8.0| ≤ 1.5| ± 8.0| ≤3.0
P5000G (P/N F144066) WITH D5000 TIPS
500| ± 12| ≤3| ± 40| ≤15.0
2500| ± 15| ≤5| ± 40| ≤15.0
5000| ± 30| ≤8| ± 40| ≤15.0
P10mLG (P/N F144067) WITH D10ML TIPS
1000| ± 30| ≤6| ± 60| ≤30.0
5000| ± 40| ≤10| ± 60| ≤30.0
10000| ± 60| ≤16| ± 60| ≤30.0
PIPETMAN G MULTICHANNEL
P8X10G (P/N F144068) P12X10G (P/N F144069) WITH D10 AND DL10 TIPS
1| ± 0.08| ≤0.05| ± 0.24| ≤0.16
5| ± 0.20| ≤0.10| ± 0.24| ≤0.16
10| ± 0.20| ≤0.10| ± 0.24| ≤0.16
P8X20G (P/N F144070) P12X20G (P/N F144071) W ITH DL10 TIPS
2| ± 0.10| ≤0.08| ± 0.4| ≤0.20
10| ± 0.20| ≤ 0.10| ± 0.4| ≤0.20
20| ± 0.40| ≤0.20| ± 0.4| ≤0.20
P8X200G (P/N F144072) P12X200G (P/N F144073) WITH D200 TIPS
20| ± 0.50| ≤0.25| ± 3.2| ≤1.2
100| ± 1.00| ≤0.40| ± 3.2| ≤1.2
200| ± 2.00| ≤0.50| ± 3.2| ≤1.2
P8X300G (P/N F144074) P12X300G (P/N F144075) WITH D300 TIPS
30| ± 1.00| ≤0.35| ± 8.0| ≤3.0
150| ± 1.50| ≤0.60| ± 8.0| ≤3.0
300| ± 3.00| ≤1.00| ± 8.0| ≤3.0

• P5000G and P10mLG do not have tip ejectors.

Setting the Volume
The volume of liquid to be aspirated is set using the volumeter. The dials are colored either black or red to indicate the position of the decimal point, depending on the model (refer to Figure 3).

To achieve maximum accuracywhen setting the volume, proceed as follows:

• When decreasing the volume setting, slowly reach the required setting, making sure to not overshoot the mark.
• When increasing the volume setting, pass the required value by 1/3 of a turn and then slowly decrease to reach the volume, making sure to not overshoot the mark.

Fitting the Tips
To fit a new PIPETMAN DIAMOND Tip, push the tip holder into the tip using a slight twisting motion to ensure a firm, airtight seal.
PIPETMAN G is equipped with a stainless steel tip ejector and can also be used with a plastic tip ejector, available as an accessory. Refer to PIPETMAN G User’s Guide LT801122, ACCESSORIES.

Fitting the Tips on P2G and P10G Models

NOTE
For the P2G and P10G models, a dual-position adapter (plastic) is required to fit DL10 tips (long tips) or D10 tips (short tips). The metallic rod of the tip ejector is shaped so the adapter may be clipped to it in either position.
P2G and P10G models are delivered with the adapter in place, ready to use DL10 tips. If D10 tips are used, the adapter must be repositioned in the shorter slot as follows:

1. Pull the adapter down from the metallic rod.
2. Turn the adapter 180°.
3. Refit the adapter so the end of the metallic rod engages the shorter slot of the adapter.

Figure 5
Dual-position adapter for P2G and P10G with stainless steel tip ejector

Ejector Spacer for PIPETMAN G 10 µL Multichannel Models

According to the tip used, D10 or DL10, you may have to exchange the ejector spacer. The broadly spaced one is for D10, and the narrowly spaced one is for DL10.

1. Remove the tip ejector, keeping both ejector locks depressed. Pull the tip ejector down.
2. Gently press the tabs from the ejector spacer and remove it from the tip ejector.
3. Insert the alternative ejector spacer and click it to the tip ejector.
4. To refit the tip ejector, gently re-insert the tip ejector vertically into the rails of the ejector support.

PIPETMAN DIAMOND Tips are best fitted with the ROCKY RACK technique, invented by Gilson, available only in our TIPACK and TOWERPACK.

PIPETMAN DIAMOND Tip Compatibility for Multichannel Models

• Using the broad ejector spacer D10, you can adapt a D10 tip (see above).

Pre-Wetting the Tips
Pre-wetting the tips before pipetting helps prepare the tips for the best pipetting performance. Ideally, the pre-wet includes both immersing the tip in the liquid and performing one pipetting step. Pre-wetting the tips helps ensure that volumes that you pipette will achieve accuracy and precision within specifications.

Aspirating

1. Press the push button to the first stop (this corresponds to the set volume of liquid).
2. Hold the pipette vertically and immerse the tip in the liquid (refer to PIPETMAN G User’s Guide LT801122).
3. Release the push button slowly and smoothly (to the top position) to aspirate the set volume of liquid.
4. Wait one second, and then withdraw the pipette tip from the liquid. You may wipe any droplets away from the outside of the tip using a medical wipe; however if you do so, take care to avoid touching the tip’s orifice.

Dispensing

1. Place the end of the tip against the inside wall of the recipient vessel (at an angle of 10° to 40°).
2. Press the push button slowly and smoothly to the first stop.
3. Wait for at least one second, then press the push button to the second stop to expel any residual liquid from the tip. Keep the push button pressed fully down and (while removing the pipette) draw the tip along the inside surface of the vessel.
4. Release the push button smoothly. Eject the tip by pressing firmly on the tip ejector button.

NOTICE
It is recommended to regularly verify the volume displayed on the dial while pipetting.

Maintenance

Routine maintenance will help keep your pipette in good condition, ensuring a continued high level of performance. Maintenance is limited to cleaning or autoclaving the parts specified in the PIPETMAN G User’s Guide LT801122, or to replacing the push button, connecting nut, tip ejector, tip holder, seal, and O-ring.

NOTICE
PIPETMAN P2G and P10G should not be disassembled. You may only replace the push button, tip ejector, and its dual-position adapter. With these pipettes, if the tip holder is damaged, the piston may also be damaged.
After replacing any parts, you should verify the performance of your pipette following the verification procedure available on the Gilson website (www.gilson.com). If the pipette needs to be readjusted, please contact your local authorized Gilson Service Center.

NOTICE
Decreased pipetting forces are due to the design of the piston assembly, which includes a very high-quality lubricant (part number 5440011070). The use of any other lubricant voids the warranty of this pipette.

Warranty

Gilson warrants this pipette against defects in material under normal use and service for a period of three years for both single channel and multichannel models from the date of purchase.
This warranty shall not apply to pipettes which are subject to abnormal use and/or improper or inadequate maintenance (contrary to the recommendations given in the user’s guide), including, but not limited to pipettes that have been subjected to physical damage, improper handling, or spillage or exposure to any corrosive environment. This warranty shall also be void in the event pipettes are altered or modified by any party other than Gilson or its designates. Gilson’s sole liability under this warranty shall be limited to, at Gilson’s sole option, repair or replacement of any defective components of pipettes or refund of the purchase price paid for such pipettes. Routine cleaning, control and recalibration are not covered under the warranty. The replacement of wearing parts such as seals, O-rings, broken pistons assembly, and broken tip holders are
not covered under the warranty.

NOTICE
Yearly routine maintenance is highly recommended to keep your pipette in good condition, ensuring a continued high level of performance.

THE FOREGOING WARRANTY IS EXCLUSIVE AND GILSON HEREBY DISCLAIMS ALL OTHER WARRANTIES, WHETHER EXPRESS OR IMPLIED, INCLUDING ANY WARRANTIES OF MERCHANTABILITY AND ANY WARRANTIES OF FITNESS FOR A PARTICULAR PURPOSE, UNDER NO CIRCUMSTANCES SHALL GILSON BE LIABLE FOR ANY CONSEQUENTIAL, PUNITIVE, INDIRECT OR INCIDENTAL DAMAGES ARISING OUT OF  ANY BREACH OF ANY EXPRESS OR IMPLIED WARRANTY.

Web Resources
Visit Gilson’s website at www.gilson.com for:

• Service contact information
• PIPETMAN G User’s Guide LT801122 pdf
• Information about accessories, spare parts, and related products
• Additional product information and special offers

NOTES_____

gilson.com/contactus
LT801624/D | ©2021 Gilson, Inc. All rights reserved.

References

• Gilson | Home | Liquid Handling, Purification and Extraction Solutions

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