BrandTech single channel pipette spare parts Owner’s Manual

Operating Manual
Conformity Declaration

Device name:  Transferpette®, Including pipette tips from BRAND
Device type: Transferpette®, Typ Variabel, Typ Fix, -8, -12 Transferpette®, Adjustable Volume, Fixed Volume, -8, -12
Art. No. : 703700, 703706, 703708, 703710, 703712, 703720, 703726, 703728, 703730, 703732, 704708, 704716, 704720, 704723, 704738, 704744, 704754, 704762, 704764, 704768, 704769, 704770, 704772, 704773, 704774, 704778, 704780, 704782, 704784, 705808, 705816, 705820, 705828, 705838, 705844, 705854, 705862, 705864, 705868, 705.869, 705870, 705872, 705873, 705874, 705878, 705880, 705882, 705884, 705900, 705905, 705908, 705910, 705912, 705920, 705926, 705928, 705930, 705932
Pipette nspftzen: Pipette Tips: all size; Art. No. : 702595, 702600, 702603, 702604, 702605, 702608, 732002, 732004, 732006, 732008, 732010, 732012, 732022, 732024, 732026, 732028, 732030, 732032, 732102, 732104, 732106, 732108, 732110, 732112, 732122, 732124, 732126, 732128, 732130, 732132, 732202, 732204, 732206, 732208, 732210, 732212, 732222, 732224, 732226, 732228, 73223), 732232, 732244, 732248, 732252, 732264, 732268, 732272, 732302, 732303, 732306, 732308, 732310, 732312,732322, 732324, 732326, 732328, 732330, 732332, 732344, 732348, 732352, 732364, 732368, 732372, 732502, 732503, 732506, 732508, 732510, 732512, 732514, 732602, 732604, 732606, 732603, 732610, 732612, 732614, 732622, 732624, 732626, 732628, 732630, 732632, 732634, 732702, 732704, 732706, 732708, 732710, 732712, 732714, 732722, 732724, 732726, 732728, 732730, 732732, 732734, 732802, 732804, 732805, 732808, 732810, 732812, 732814, 732822, 732824, 732826, 732828, 732834, 732832, 732834

single channel pipette spare parts

Registration No.:
EN ISO 8655 Teil 2 cinch!.  (Piston-operated volumetric apparatus -Piston pipettes according DIN EN ISO 8655 Part 2 incl, Pipette tips for In-Vitro applicatson)
Manufacturer: BRAND GMBH + CO KG
Address: Otto-Schott-Str. 25, 97877 Wertheim • Germany

The devices named below are developed, constructed and manufactured in accordance to the fundamental requirements of the EC directive 98/.79/EC (IVO). This declaration of conformity Is issued under the sole responsibility of the manufacturer.

Scope of supply

Transferpette® S, adjustable / fixed-volume, DE-M marking, supplied with quality certificate, shelf mount and silicone grease.

Terms of use

• Please carefully read the operating manual before using the instrument for the first time.
• The operating manual is part of the instrument and must be kept in an easily accessible place.
• Be sure to include the operating manual if possession of this instrument IS transferred to a third party.

2.1 Hazard levels
The following signal words identify possible hazards:

2.2 Icon

Safety regulations

3.1 General safety regulations
Please read carefully!
This instrument can be used in combination with hazardous materials, work processes and equipment. However, the operating manual cannot cover all of the safety issues that may occur in doing so. It is the user’s responsibility to ensure compliance with the safety and health regulations and to specify the corresponding restrictions before use.

1. Every user must read and observe this operating manual before using the instrument.

2. Follow the general hazard instructions and safety regulations, e.g. wear protective clothing, eye protection and protective gloves.
   When working with infectious or hazardous samples, the standard laboratory rules and precautions must be adhered to.

3. Follow the instructions given by the reagent manufacturer.

4. Use the instrument only for pipetting liquids within the defined limitations and restrictions of use.
   Comply with the operating exclusions; see p. 40.
   In case of doubt,  contact the manufacturer ordealer.

5. Always perform work in a manner that does not endanger users or other people. Avoid splattering. Use only suitable vessels.

6. Avoid touching the tip opening when working with aggressive media.

7. Never use force.

8. Use only original spare parts. Do not make any technical modifications.
   Do not disassemble the  instrument further than described in the user manual!

9. Always check that the instrument is in proper working condition before use. If instrument malfunctions are indicated (e.g. sluggish pistons, leaks), stop pipetting immediately and refer to the section “Troubleshooting”; see p. 57. Contact the manufacturer, if necessary.

3.2 Purpose
This is an air displacement pipette for pipetting aqueous solutions of medium density and low to medium viscosity.
3.3 Limitations of use
This instrument is intended for pipetting samples, within the following limitations:

• Operating temperature of instrument and reagent should be between +15 °C and +40°C (59°F to 104°F) (other temperatures upon request)
• Vapor pressure up to 500 mbar
• Viscosity: 260 mPa s

3.4 Operating limitations
Viscous and wetting liquids may compromise volumetric accuracy. Volumetric accuracy may also  be affected when pipetting liquids whose temperature deviates from the
ambient temperature by  more than ± 1 °C/ ± 1.8 °F.

3.5 Operating exclusions
The user is responsible for checking the compatibility of the instrument with the intended application. The instrument cannot be used:

• for liquids that corrode polypropylene
• for liquids that corrode polycarbonate (viewing window)
• for liquids that corrode FKM and polyether ether ketone (PEEK)
• for liquids that corrode polyvinylidene fluoride
• for liquids that corrode polyphenylsulphide (PPS) (on adjustable 50 µl instrument)
• for liquids with very high steam pressure

Functions and controls

1 Pipetting key
3 Easy Calibration function
5 Pipetting shaft
7 Hand grip
9 Volume-change protection| 2 Volume-setting wheel
4 Finger rest
6 Tip cone
8 Volume display
10 Tip ejection key
---|---

Label window

The pipette can be individually labeled on the finger rest:
a. Remove the label window on the finger rest.
b. Mark the labeling film.
c. Reinsert the labeling film with window.

Pipetting

1. Inserting tips
   NOTICE
   Ø 2 ml, 5 ml and 10 ml instruments should only be used with a built-in PE filter; see p. 53.
   Ø Perfect analysis results can only be achieved by using quality tips.
   We recommend BRAND pipette tips. For additional information, refer to the accuracy table p. 48.
   Ø Pipette tips are disposable products! a. Use the correct tips, in ac- cordance with the volume range or color code! Make sure that the tips are firmly in place and leak tight.

2. Setting the volume a. Slide the volume-change protection upward (UNLOCK).
   b. Turn the volume-setting wheel to select the desired volume. In doing so, turn the adjustment wheel steadily, avoiding abrupt turning motions.
   c. Slide the volume-change protection downward (LOCK). The volume-setting wheel becomes noticeably more difficult to turn, but movement is not completely blocked.

3. Aspirating a sample
   NOTICE
   The ISO 8655 standard requires that pipette tips are pre-wettedonce before the actual pipetting procedure. a. Press the pipetting key until first resistance is felt.
   b. Hold the instrument vertically and immerse the tip in the liquid.
   c. Allow the pipetting button to steadily move back to its original position.
   Leave the tip immersed in the liquid for a few seconds, so that the set volume is aspirated completely. This is especially important when pipetting viscous media and when using pipettes with large volumes. Volume range| Immersion depth [mm]| Wait time [s]
   ---|---|---
   0.1 µl – 1 µl| 2-Jan| 1

   1 µl – 100 µl| 3-Feb| 1
   100 µl – 1,000 µl| 4-Feb| 1
   1,000 µl| 6-Mar| 3

NOTICE
Do not lay the instrument down when the tip is filled; this can cause the medium to flow into the instrument and contaminate it! The instrument should always be stored
in the provided shelf  mount or table stand and kept in an upright position, without any tip inserted.

4. Dispensing a sample a. Place the pipette tip against the vessel wall. Hold the pipette at an angle of 30-45° to the vessel wall.
   b. Press the pipetting button at a uniform speed until the first resistance is felt and hold it. To improve accuracy, comply with the corresponding  waittime for serums, highly-viscous or low-density media. c. Completely empty the tip by over-stroking: press the pipetting button until the second resistance is felt.
   d. While doing this, wipe the pipette tip against the vessel wall.
   e. Remove the pipette tip from the vessel wall and allow the pipetting button to move back to its original position.

5. Ejecting a tip
   NOTICE
   The instrument should always be stored in the provided shelf mount or table stand and kept in an upright position, without any tip inserted. a. Hold the pipette shaft over a suitable disposal bin and press the tip ejection button all the way down.
   

Checking the volume

We recommend testing the instrument every 3 to 12 months, depending on the levelof use.
However, the testing cycle  can be adapted to meet individual requirements.
Gravimetric volume  testingof the  pipette is carried out according  to the following steps and complies  with DIN EN ISO 8655, Part

1. Setting the nominal volume Set the maximum specified instrument volume.
2. Conditioning the pipette Condition the pipette before testing by aspirating and dispensing the test liquid (H2O distilled) with a pipette tip five times.
3. Performing the test

NOTICE
In accordance with DIN EN ISO 8655-2, a tip change is recommended after each individual measurement.
An exception to this  rule can be made,  according to DAkkS guideline DKD-R8-1.
a. Aspirate the test liquid and pipette into the weighing vessel.
b. Weigh the pipetted amount with an analysis scale. (Please refer to the user manual of the scale manufacturer.)
c. Calculate the pipetted volume. In doing so, take into account the temperature of the test liquid.
d. At least 10 pipetting series and weighings in 3 volume ranges (100%, 50%, 10%) are recommended.

Calculation (for nominal volume)

*) = Calculation for accuracy (A%) coefficient of variation (CV%): A% and CV% are calculated using the formulas of statistical quality control.

Accuracy table

Transferpette®S, adjustable

Volume
range
[µl]| Partial
volume
[µl]| A  ± %| CV  %≤| Sub
steps
[µl]| Recommended tip
type
[µl]
---|---|---|---|---|---
0.1 – 1| 1
0.5
0.1| 2
4
20| 1.2
2.4
12| 0.001| 0.1 – 20
0.1 – 2.5| 2.5
1.25
0.25| 1.4
2.5
12| 0.7
1.5
6| 0.002| 0.5 – 20
0.5 – 10| 10
5
1| 1
1.6
7| 0.5
1
4| 0.01| 0.5 – 20
20-Feb| 20
10
2| 0.8
1.2
5| 0.4
0.7
2| 0.02| 2 – 200
May-50| 50
25
5| 0.8
1.2
4| 0.3
0.5
2| 0.05| 2 – 200
10 – 100| 100
50
10| 0.6
0.8
3| 0.2
0.4
1| 0.1| 2 – 200
20 – 200| 200
100
20| 0.6
0.8
3| 0.2
0.3
0.6| 0.2| 2 – 200
100 – 1,000| 1,000
500
100| 0.6
0.8
3| 0.2
0.3
0.6| 1| 50 – 1,000
500 – 5,000| 5,000
2,500
500| 0.6
0.8
3| 0.2
0.3
0.6| 5| 500 – 5,000
1000 – 10,000| 10,000
5,000
1,000| 0.6
0.8
3| 0.2
0.3
0.6| 10| 1,000 – 10,000

*A = Accuracy, CV = Coefficient of Variation

Transferpette®S, fixed-volume

Volumerange [µl]| ≤ ± %| CV  % ≤| Recommended tip type [µl]
---|---|---|---
    10| 1| 0.5| 0.5 – 20
    20| 0.8| 0.4| 2 – 200
    25| 0.8| 0.4| 2 – 200
    50| 0.8| 0.4| 2 – 200
  100| 0.6| 0.2| 2 – 200
  200| 0.6| 0.2| 2 – 200
  500| 0.6| 0.2| 50 – 1,000
1,000| 0.6| 0.2| 50 – 1,000
2,000| 0.8| 0.3| 500 – 5,000

*A = Accuracy, CV = Coefficient of Variation

20 °C
Ex

Final test values based on the nominal volume (= max. volume) printed on the instrument and the specified partial volumes at the same temper-ature (20 °C/68 °F) of the instrument, surroundings and distilled water, in accordance with DIN EN ISO 8655.

Adjustment – Easy Calibration

The instrument is permanently calibrated for aqueous solutions. If it is determined that the pipette is operating inaccurately or to adjust the instrument to work with solutions
of varying density and viscosity or  with specially-shaped pipette tips, it can  be calibrated using theEasy Calibration Technique.

a. Perform a volume check and determine the actual value; see p. 46.
b. Remove label window (1) and labeling film (2): Gently move the clamp and lift it off.
c. Using a paper clip or an unused pipette tip, remove the protective film (3) (the protective film can be discarded). d. Slide the red adjustment slider back completely, lift the volume-setting wheel (decoupling) and release the adjustment slider.

e. Set the adjustment value:
Transferpette® S, adjustable: with the volumesetting wheel  inthe UNLOCK position, set to  the previouslydetermined actuavalue.
Transferpette® S, fixed-volume: set the volume  by rotating in the +/- direction.
A volume check is recommended after every adjustment. f. Slide the adjustment slider completely back again, push the volume-setting wheel downward and release the adjustment slider. Re-attach the labeling film and reassemble the
label window.

NOTICE
The change to factory settings is indicated by the red adjustment slider now visible in the label window.

Cleaning and disinfection

9.1 Autoclaving
The Transferpette® S and S -8/-12 are completely autoclavable at 121 °C (250 °F), 2 bar and a holding time of at least 15 minutes, in accordance with DIN EN 285.

NOTICE
The effectiveness of autoclaving must be verified by the user. Maximum safety is achieved through vacuum sterilization.
We recommend the use of sterilization bags.

NOTICE
Prior to autoclaving, the volume-setting wheel must be set on an available numbered value (e.g., 11.25 or 11.26, but not between), with the volume-change protection set to fully unlocked (UNLOCK).
If the pipette is autoclaved frequently, the piston and seal should be greased with the supplied silicone grease in order to ensure proper movement. After autoclaving, tighten the connection between the hand grip and the pipette shaft if necessary.
a. Eject the pipette tip.
b. Autoclave the complete pipette without any further disassembling.
c. Allow the Transferpette® S or S -8/-12 to completely cool and dry.

9.2 UV sterilization
The instrument is resistant to normal exposure to a UV sterilization lamp. The effects of the UV exposure may cause some color change.
Filter Transferpette® S 2 ml, 5 ml + 10 ml
A hydrophobic PE filter is used to prevent liquid from entering the pipette.
Change the filter if it becomes wet or contaminated.
a. Use a flat object, such as a screwdriver.
b. Remove the filter carefully, without damaging the tip cone.
Remove the filter before autoclaving!
The instrument can also be operated without a filter.

Maintenance

10.1 Disassembling/cleaning (Transferpette® S up to 1,000 µl)
a. Check the pipette tip cone for damage.
b. Inspect the piston and seal for contamination.
c. Check the pipette for leaks.
We recommend using BRAND’s leak detector, the BRAND PLT unit.
As an alternative to this, aspirate a sample and hold the pipette vertically for approx. 10 s. If a drop  orms on the pipette tip, refer to p. 57.

Cleaning
a. Detach the pipette shaft (S) from the hand grip by unscrewing it.
b. Unscrew the upper part of the ejector unit (A) from the pipette shaft.
c. Pull out the shaft (B, C and D) from the lower part of the ejector unit (E).
d. Unscrew the piston unit (B).![BrandTech single channel pipette spare parts

• Cleaning](https://manuals.plus/wp-content/uploads/2022/11/BrandTech-single- channel-pipette-spare-parts-Cleaning.jpg)

NOTICE
Do not disassemble the piston unit (B) any further!
e. Remove the spring (C) (not possible on Transferpette® S 1 µl, 2.5 µl and 10 µl!).
f. Clean the parts shown with a soap solution or isopropanol, and then rinse with  distilledwater.
g. Dry the parts (max. 120 °C/248 °F).
h. Grease piston and seal with a very thin layer of supplied silicone grease.
Reassemble the cooled parts in reverse order. Only handtighten the piston unit and theupper part of the ejector unit (A, B).

10.2 Disassembling/cleaning (Transferpette® S 2 ml – 10 ml)
a. Check the pipette tip cone for damage.
b. Inspect the piston and seal for contamination.
c. Check the pipette for leaks.
We recommend using BRAND’s leak detector, the BRAND PLT unit. As an alternative to this, aspirate a sample and hold the pipette vertically for approx.
10 s. If a drop forms  on the pipette  tip, refer to p. 57.

Cleaning

a. Remove the entire shaft (S) from the hand grip by rotat-ing at the upper end of the ejector (F) and remove the filter (K) from the bottom part of the shaft (H).
b. Separate the bottom part of the ejector (F’) by unscrewing it from  the upper part of the ejector (F).
c. Unscrew and dismantle the piston unit (G) with the ejector spring (I) and the  bottom part of the shaft (H).
d. Remove the O-ring-seal from the piston unit and clean it.
NOTICE
Do not disassemble the piston unit (G) any further!
e. Clean the piston unit (G) and the bottom part of the shaft (H) with a soap solution or isopropanol, and  then rinse with distilled water.
f. Dry the parts (max. 120 °C/248 °F) and allow them to cool.
g. Carefully lubricate the inside and outside of the O-ring (O) and mount it on the piston.
Reassemble the individual com- ponents in reverse order.

Troubleshooting

The instrument does not aspirate or aspirates too little; the dispensed volume is too low| Seal contaminated| Clean seal
Seal or cone is damaged| Replace seal or shaft
Piston is contaminated or damaged| Clean or replace piston
Aspiration is very slow| Shaft is clogged| Clean shaft
Filter contaminated on 2 ml, 5 ml or 10 ml instruments| Change filter
Dispensed volume too large| Pipetting button pressed too far (to the over- stroke point) before aspirating| Ensure proper handling.
Piston sluggish| Piston is contaminated or not greased| Clean piston and apply grease

Product markings

set out in the EC Directives and has been subjected to the specified testing procedures.
| The instrument is marked in accordance with the German Weights and Measures Act and  the Weights and Measures Ordinance.
Character sequence DE-M (DE  for Germany), framed by a rectangle, as  well as the two last digits of the year the marking
was added (here: 2018).
| Autoclavable up to the temperature shown

Ordering Information

13.1 Order info/accessories
Transferpette ®S, fixed-volume

Transferpette® S, adjustable

Benchtop rack for 6 Transferpette®  S or 6
Transferpette® S -8/-12| Wall mount for 1 Transferpette®  S or 1
Transferpette® S -8/-12| Shelf mount for 1 Transferpette S or 1
Transferpette ® S -8/-12
---|---|---
| |
Order No. 7048 07| Order No. 7048 12| Order No. 7048 11

13.2 Spare parts
13.2.1 Transferpette® S up to 1,000 µl
The appearance and dimensions of the spare parts correspond to the respective nominal volume. (Fig. spare parts for Transferpette® S 20-200 µl)

A Ejector (upper part)
C Seal with spring
E Ejector (lower part)| B Piston unit
D Shaft with ejector spring
---|---

Transferpette® S, fixed-volume

Transferpette® S, adjustable

• Seal permanently installed in shaft – not removable!

13.2.2 Transferpette® S, 2 ml, 5 ml and 10 ml
The appearance and dimensions of the spare parts correspond to the respective nominal volume. (Fig. replacement parts for Transferpette® S 5 ml).

F Ejector (upper part)
G Piston unit
I Ejector spring| F’ Ejector (lower part)
H Shaft (lower part)
O O-ring
---|---

Transferpette® S, fixed-volume and adjustable

13.3 Additional accessories

14.1 Sending for repairs
NOTICE
Transporting hazardous materials without approval is prohibited by law.

Clean the instrument thoroughly and decontaminate!

• When returning products, please enclose a general description of the type of malfunction and the media used. The instrument cannot be repaired if information about the media used is not pro- vided.
• Only send the instrument without a battery installed.
• The instrument is returned at the risk and expense of the sender.

Outside USA and Canada
Fill out the “Declaration on the Absence of Health Hazards” and sent it together with the instrument to the manufacturer or dealer.
Within USA and Canada
Send only cleaned and decontaminated instruments to the address, which you received together with the return number. Attach the return number in a clearly visible place on the package.

Calibration service

The ISO 9001 and GLP guidelines require regular inspection of your volume measuring instruments. We recommend performing a volume check every 3 to 12 months. The cycle is dependent on the individual requirements of the instrument. Checks should be performed more frequently, in case  of highfrequency of use or the use of aggressive me-dia.
BRAND also offers the possibility to have your instruments calibrated by our factory calibration service or by the BRAND DAkkS laboratory. Simply send us the instrument to be calibrated, accompanied by details about which type of calibration you would like. The instrument will be returned to you after a few days together with a test report (factory calibration) or a DAkkS calibration certificate.
More information can  be obtained from your dealer  or directly from BRAND.

Warranty

We shall not be liable for the consequences of improper handling, use, servicing, operating or unauthorized repairs of the device or for the consequences of normal wear and tear, especially of wearing parts such as pistons, seals, valves and the breakage of glass. The same applies for failure to follow the instructions of the operating manual. We are not li-able for damage resulting from disassembly beyond that described in the operating manual or if non-original spare parts or components have been installed.

Disposal

For the disposal of the intrument and pipette tips, please follow the respective national disposal regulations.

Read User Manual Online (PDF format)

Read User Manual Online (PDF format)  >>

Download This Manual (PDF format)

Download this manual  >>