HACH PHC281 Water Quality Laboratory Refillable pH Electrode User Manual

DOC022.53.80024
PHC281
05/2021, Edition 3
User Manual

Section 1 Product overview

The Intellectual PHC281 series pH probes are digital, combination electrodes that measure the pH of water samples. The probes are refillable and have a built-in temperature sensor. The probes have good performance in low ionic strength (LIS) samples (e.g., deionized, reverse osmosis, distilled) and other clean waters (specific conductance less than  100 µS/cm to ultrapure). The open reference junction lets the electrolyte flow at a high rate for optimum electrical connection with the sample. The open reference junction does not become clogged with suspended solids if used in dirty applications. A 59-mL bottle of 2.44 M KCl reference electrolyte filling solution is supplied with the probe. Refer to Figure 1.
Note: Do not use the probe for measurements in organic solvents.

Figure 1 Probe overview

Probe soaker bottle holder
3| Filling-hole plug| 7| Electrode filling and storage solution
4| Protective tape and filling hole| 8| Dispensing cap

Section 2 Specifications

Specifications are subject to change without notice.

built-in temperature sensor
pH range| 0 to 14 pH
pH accuracy| ±0.02 pH
Reference type| Ag/AgCl
Reference junction| Open
Slope| –59 mV/pH (90 to 110% at 25 °C (77 °F) per Nernstian theoretical value)
Isopotential point| 0 (±30) mV at 7.0 (±0.5) pH
Sodium (alkalinity) error| –0.6 pH at pH 12.6 in 1 M NaOH
Specifications| Details
---|---
Temperature accuracy| ±0.3 °C (±0.54 °F)
Temperature sensor type| 30 kΩ NTC thermistor
Operating temperature| 0 to 50 °C (32 to 122 °F)
Storage temperature| 5 to 40 °C (41 to 104 °F)
Minimum immersion depth| 15 mm (0.59 in.)
Body material (standard)| Zeonor™
Electrolyte filling solution| 2.44 M KCl
Storage solution| 2.44 M KCl1
Cable connection| M12 digital output and connector
Dimensions| Diameter: 12 mm (0.47 in.)
Length: 175 mm (6.9 in.) total; 103 mm (4.1 in.) below headCable length: PHC28101: 1 m (3.3 ft); PHC28103: 3 m (9.8 ft)
Weight (includes cable)| PHCxxx01: ~0.4 kg (0.9 lb); PHCxxx03: ~0.45 kg (1 lb)
Warranty| 1 year on the probe. This warranty covers manufacturing defects, but not improper use or wear.
Certifications| CE, FCC/ISED

Section 3 Safety information

3.1 Intended use
The Intellectual probes are intended for use by individuals who measure water quality parameters in the laboratory. The Intellectual probes do not treat or alter the water.
3.2 Use of hazard information

Indicates a potentially or imminently hazardous situation that, if not avoided, will result in death or serious injury.

Indicates a potentially or imminently hazardous situation that, if not avoided, could result in death or serious injury.

Indicates a potentially hazardous situation that may result in minor or moderate injury.

N O T I C E

Indicates a situation that, if not avoided, may cause damage to the instrument. Information that requires special emphasis.

1 Use of other storage solutions can cause permanent damage to the probe.

3.3 Precautionary labels
Read all labels and tags attached to the instrument. Personal injury or damage to the instrument could occur if not observed. A symbol on the instrument is referenced in the manual with a precautionary statement.

| Electrical equipment marked with this symbol may not be disposed of in European domestic or public disposal systems. Return old or end-of-life equipment to the manufacturer for disposal at no charge to the user.
---|---

3.4 Product hazards

****| Chemical exposure hazard. Obey laboratory safety procedures and wear all of the personal protective equipment appropriate to the chemicals that are handled. Refer to the current safety data sheets (MSDS/SDS) for safety protocols.

****| Chemical exposure hazard. Dispose of chemicals and wastes in accordance with local, regional, and national regulations.

****| Personal injury hazard. Glass components can break. Handle with care to prevent cuts.

Section 4 Preparation for use

N O T I C E

Make sure to remove the protective tape from the filling hole of new probes. A probe with a blocked filling hole will not operate correctly.

New probes are filled with electrolyte filling solution and have a soaker bottle that contains storage solution to keep the glass bulb and reference junction hydrated. Prepare the probe for calibration and measurement as follows.

1. Remove the protective tape from the filling hole. Refer to Figure 2.

2. Rinse the reference junction and glass bulb with deionized water. Blot dry with a lint-free cloth.

3. If the inner filling solution is low, add more filling solution. Refer to Fill the probe on page 12.

4. Make sure that the meter has the correct date and time settings. The service-life time stamp in the probe comes from the date and time settings in the meter.
   Note: Some meters automatically open the date and time settings when the meter starts for the first time, or after battery replacement.

5.  Connect the probe to the meter.

6. Open the filling hole before use. Keep the level of the electrolyte filling solution above the level of the measurement liquid during use. Refer to Figure 3.
   Note: For faster stabilization, soak the probe for 3 or more minutes in the sample before use.

Figure 2 Remove the protective tape

Figure 3 Probe position during use

Section 5 Calibration

The procedure that follows is applicable to meters that can connect to Intellical pH probes. Refer to the applicable meter documentation for meter operation and probe-specific settings.

5.1 Calibration notes

Read the notes that follow before calibration:

• Use prepared pH buffer solutions or mix pH buffer powder pillows with deionized water for Discard the prepared buffer solutions after each calibration.
• Use two or three buffer solutions for best results. Two buffer solutions are sufficient if the expected sample pH is between the pH of the two buffer solutions. The sequence in which the pH buffer solutions are used is not important. Use buffer solutions that are 2 or more pH units apart.
• For a one-point calibration, use a pH buffer near the expected sample pH.
• Use the default calibration options or change the options in the probe settings menu.
• Use the single display mode for calibration when more than one probe is connected to the meter (if applicable).
• Calibrate the probes and verify the calibration regularly for best results. Use the meter to set calibration reminders.
• The calibration data is stored in the probe. When a calibrated probe is connected to a different meter with the same calibration options, a new calibration is not necessary.
• Air bubbles below the sensor when in the solution can cause a slow response or error in the Make sure to remove air bubbles during calibration.
• The pH buffer solutions have known pH values at different temperatures. The meter uses the mV and temperature readings of the probe in the pH buffer solutions to calculate a calibration slope. During measurements, the meter adjusts the slope for the sample temperature to determine the pH value of the sample.

5.2 Calibration procedure

| | |
---|---|---|---
1. Go to the calibrate menu. Select the probe, if applicable. The display shows the pH buffer solutions to use for calibration.| 2. Prepare or pour the pH buffer solutions into different beakers.| 3. Open the filling hole.| 4. Rinse the probe with deionized water. Dry the probe with a lint-free cloth.
| | |
---|---|---|---
5. Put the probe in the first pH buffer solution.  Make sure that the sensor and reference junction is fully in the solution. Do  not put the probe on the bottom or sides of
the beaker.| 6. Shake the probe from side to side to refresh the reference junction and remove
air bubbles.| 7. Stir slowly, then read the pH value of the buffer solution. The display shows the temperature corrected pH value when the reading is stable.| 8. Continue with steps 4 through 7 for the remaining buffers or select Done.

9. Save the calibration.

Section 6 Sample measurement

The procedure that follows is applicable to meters that can connect to Intellical pH probes. Refer to
the applicable meter documentation for meter operation and probe-specific settings.

6.1 Sample measurement notes

Read the notes that follow before sample measurements.

• Rinse the probe with deionized water and dry with a lint-free cloth between measurements to prevent contamination.
• If complete traceability is necessary, enter a sample ID and operator ID before measurement. Refer to the meter manual for instructions.
• The meter automatically saves the measurement data when the user manually reads each data point and when the meter is set to read at regular intervals. The user must manually save each data point when the meter is set to read continuously.
• Air bubbles below the sensor can cause a slow response or error in the measurement. Make sure to remove air bubbles before and during measurements.

6.2 Sample measurement procedure

| |
---|---|---
1. Collect the sample.| 2. Open the filling hole.| 3. Rinse the probe with deionized water. Dry the probe with a lint-free cloth.
| |
---|---|---
4. Put the probe in the sample with the sensor and reference junction fully in the sample. Do not put the probe on the bottom or sides of the beaker.| 5. Shake the probe from side to side to refresh the reference junction and remove air bubbles.| 6. Stir gently, then read the pH value of the sample. The display shows the temperature-compensated  H value when the reading is a table.

Section 7 Verify the calibration

Measure the pH value of a fresh pH buffer solution to make sure the result is accurate. The meter compares the selected pH buffer value to the measured pH value and accepts or rejects the measurement. The user can change the pH buffer solution and acceptance criteria for verification in the probe-specific settings.

Note: Password protection may prevent access to the acceptance criteria.

7.1 Verification procedure

| | |
---|---|---|---
1. Go to the verification menu. The display shows the pH buffer solution to use for verification.
Note:   menu name for HQd meters: Run check standard.| 2. Prepare or pour the pH buffer solution into a beaker.| 3. Open the filling hole.| 4. Rinse the probe with deionized water.
Dry the probe with a lint-free cloth.
| |
---|---|---
5. Put the probe in the pH buffer solution with the sensor and reference junction fully in the solution. Do not put the probe on the bottom or sides of the beaker.| 6. Shake the probe from side to side to refresh the reference junction and remove
air bubbles.| 7. Stir gently, then read the pH value of the buffer solution. The meter accepts
or rejects the result.

Section 8 Maintenance

Regular maintenance is necessary for the best accuracy, stabilization time, and life of the probe.
Keep the probe in the recommended storage solution between measurements.

8.1 Clean the probe

N O T I C E

Probes with an open reference junction can become permanently damaged if the reference junction is soaked for a long time in a cleaning solution. Make sure to soak the probe below the reference junction only.

Clean the probe regularly to remove contamination and to keep the reference junction open.

Symptoms of contamination:

• Incorrect or irregular readings
• Slow stabilization times
• Calibration errors
• Sample material stays on the probe

1. Rinse the probe with deionized water. Use warm (35–45 °C (95–113 °F)) deionized water to remove the storage solution that dries on the probe. Dry the probe body with a lint-free cloth.
2. Soak the probe below the reference junction in the applicable cleaning solution for the specified time. Do not let the reference junction soak in the cleaning solution or the probe can become permanently damaged. Refer to Figure 4, Table 1, and Consumables on page 15.
3. Rinse or soak the probe for 1 minute in deionized water. Dry the probe body with a lint-free cloth.
4. Soak the probe in pH 4 buffer for 20 minutes.
5. Rinse the probe with deionized water. Dry the probe body with a lint-free cloth.

Figure 4 Soaking depth for open junction probes

Table 1 Cleaning solution

Contamination| Cleaning solution| Active component| Soak time
---|---|---|---
General contamination| Electrode cleaning solution for regular maintenance| KATHONTM CG, DECONEX®11| 12-16 hours
Minerals| Electrode cleaning solution for minerals/inorganic contamination| Phosphoric acid (-10%)| 10-15 minutes
Fats, grease, and oils| Electrode cleaning solution for fats, oils, and grease contamination| KATHON TM CG, TRITON® X| 2 hours maximum
Proteins| Electrode cleaning solution for proteins/organic contamination| Pepsin in HCI| 3 hours maximum
Wastewater and organic compounds| Electrode cleaning solution, extra strong| Sodium hypochlorite| 5-10 minutes

8.2 Fill the probe

Add electrolyte filling solution to the probe regularly to make sure that the electrolyte flows from the probe to the sample. Refer to Figure 5. Make sure to use the correct filling solution (2.44 M KCI).

Note: If the dispensing tip becomes clogged, remove the dispensing tip and soak the tip in warm water. Then, fully dry and assemble the tip again.

Figure 5 Fill the probe

8.3 Replace the filling solution

If the filling solution becomes contaminated, replace the filling solution.

1. Tilt the probe and open the filling hole.
2. Use a plastic transfer pipet to remove the contaminated solution from the filling hole. Discard the solution.
3. Rinse the inner probe three times with deionized water.
4. Rinse the inner probe three times with a new filling solution.
5. Fill the probe with a new filling solution. Refer to Fill the probe on page 12.

8.4 Soak procedure for dry probes

If the glass bulb becomes dry, complete the steps that follow to hydrate the probe.

1.  Soak the probe tip in pH 4 and pH 7 buffer solutions for 5 minutes in each solution.
2. Rinse the probe with deionized water. Blot dry with a lint-free cloth.
3.  Calibrate the probe.

8.5 Storage

N O T I C E

Probes can become permanently damaged if kept in a storage solution that is not specified by the manufacturer. Use only the specified storage solution (2.44 M KCl).

Do not store the probe in deionized water or in samples of low ionic strength. For short-term storage, the probe can stay in the sample for a maximum of 2 hours if the sample pH is not high. Close the filling hole and put the soaker bottle that contains the storage solution on the probe when not in use. Refer to Figure 6. Keep the probe in a vertical position with the sensor and reference junction below the liquid level in the soaker bottle. Add storage solution to the soaker bottle if necessary.

Figure 6 Probe storage

Section 9 Troubleshooting

Keep the probe clean and in the recommended storage solution when not in use for the best accuracy, stabilization time, and life of the probe.

Problem

| Possible cause| Solution
---|---|---
Decreased probe performance causes slow stabilization and prevents accurate calibrations or measurements.| The glass sensor is dirty.| Clean and condition the probe. Refer to Clean the probe on page 11.
The filling solution has contamination.| Replace the filling solution. Refer to Replace the filling solution on page 13.
The probe is not conditioned to the sample sufficiently.| Condition the probe. Refer to Preparation for use on page 5.
The glass sensor has become dry.| Soak the probe tip in buffer solutions. Refer to the Soak procedure for dry probes on page 13.
The calibration slope of the probe has changed.| Increase the accepted slope limit settings if possible, or contact technical support.
Sample properties cause slow stabilization or inaccurate measurements.| The sample absorbs carbon dioxide (CO2) from the air, which causes the pH value to slowly decrease in low ionic strength (LIS) or high purity samples.| Use the LIS chamber for LIS/high purity samples to prevent CO2 absorption.
The sample temperature is low, or there is a large temperature difference between samples.| Increase the sample temperature or adjust the temperature of different samples to be the same (within 2 °C (3.6 °F)).
The procedure problem causes slow stabilization and prevents accurate calibrations or measurements.| The filling hole is closed.| Open the filling hole during use.
Air bubbles are around or below the probe tip.| Carefully tap or shake the probe to remove air bubbles.
The stirring speed is too slow or too fast.| Try a different stir speed.
An incorrect buffer solution was used or the buffer solution has contamination.| Use the specified buffer solutions of good quality.
The protective tape was not removed from the filling hole.| Remove the tape from the filling hole. Refer to Preparation for use on page 5.

Section 10 Consumables

Note: Product and Article numbers may vary for some selling regions. Contact the appropriate distributor or refer to the company website for contact information.

2975149
Electrode cleaning solution for proteins/organic
contamination| 250 mL| C20C370
Electrode cleaning solution for fats, oils, and grease
contamination| 500 mL| 2964449
Electrode cleaning solution, extra strong| 250 mL| S16M002

10.1 Recommended standards

pH 4.01 and pH 7.00 buffer solution kit, Singlet one-use
packets, 20 mL each| 2 x 10/pkg| 2769920
pH 7.00 and 10.01 buffer solution kit, Singlet one-use packets, 20 mL each| 2 x 10/pkg| 2769820
pH color-coded buffer solution kit (NIST), 500 mL, includes:| 1| 2947600
   pH 4.01 ± 0.02 pH buffer (NIST)| 500 mL| 2283449
   pH 7.00 ± 0.02 pH buffer (NIST)| 500 mL| 2283549
   pH 10.01 ± 0.02 pH buffer (NIST)| 500 mL| 2283649
Powder pillows:
   pH 4.01 ± 0.02 pH buffer powder pillow (NIST)| 50/pkg| 2226966
   pH 7.00 ± 0.02 pH buffer powder pillow (NIST)| 50/pkg| 2227066
   pH 10.01 ± 0.02 pH buffer powder pillow (NIST)| 50/pkg| 2227166
Radiometer Analytical (IUPAC Series certified pH standards):
   pH 1.679 ± 0.010 at 25 °C (77 °F)| 500 mL| S11M001
   pH 4.005 ± 0.010 at 25 °C (77 °F)| 500 mL| S11M002
   pH 6.865 ± 0.010 at 25 °C (77 °F)| 500 mL| S11M003
   pH 7.000 ± 0.010 at 25 °C (77 °F)| 500 mL| S11M004
   pH 9.180 ± 0.010 at 25 °C (77 °F)| 500 mL| S11M006
   pH 10.012 ± 0.010 at 25 °C (77 °F)| 500 mL| S11M007
   pH 12.45 ± 0.05 at 25 °C (77 °F)| 500 mL| S11M008
   pH buffer 1.09, technical| 500 mL| S11M009
   pH buffer 4.65, technical| 500 mL| S11M010
   pH buffer 9.23, technical| 500 mL| S11M011

²Larger quantities are available for some items.
³ Larger quantities are available.

10.2 Accessories

HACH COMPANY World Headquarters
P.O. Box 389, Loveland, CO 80539-0389 U.S.A.
Tel. 970-669-3050
800-227-4224 (the U.S.A. only)
Fax 970-669-2932
orders@hach.com
www.hach.com

© Hach Company/Hach Lange GmbH, 2010, 2013, 2021. All rights reserved.

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