Thermo Fisher Scientific DM00113 Remove non Autoclavable Filters Instruction Manual

Thermo Fisher Scientific DM00113 Remove non Autoclavable Filters

For research use only. Not for use in diagnostic procedures. Information in this document is subject to change without notice.

Revision history DM00113

replacing them with new autoclavable filters.

Important Licensing Information
These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
Manufacturer: Life Technologies Corporation | 345 E. Mountain | Fort Collins, CO 80524.
Trademarks
All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. © 2023 Thermo Fisher Scientific Inc. All rights reserved.

About this guide

This user guide is for laboratory personnel who need to remove non- autoclavable filters that have CPVC fittings on the inlet and outlet ports and install new autoclavable filters with luer lock fittings in the Bigfoot Cell Sorter

Standard items included in the kit

• Two 500 cm2, 0.2 μm filters with luer lock fittings, ETO Sterilized, (PL00383) for use on the Decon and Clean lines
• Two 500 cm2, 0.05 μm filters with luer lock fittings, ETO Sterilized, (PL00382) for use on the DI and Sheath lines

Additional materials needed

• 3 mm Allen Key
• 70% EtOH
• Flangeless nut tightening wrench
• Disposable container for used filters
• Paper towels
• Permanent marker
• Nitrile gloves (at least four pair)

Safety

Consult the Bigfoot™ Spectral Cell Sorter User Guide for comprehensive safety information. To avoid contact with possible biological contamination and bulk fluids, use personal protective equipment (PPE) including but not limited to:

• Closed toe shoes and/or shoe booties
• Lab coat
• Gloves (nitrile)
• Eye protection (Safety glasses with side shields

Chemical and biosafety Information

When biohazardous samples and waste are present, adhere to the following guidelines and comply with any local guidelines specific to your laboratory and location.

A responsible body at the customer site must ensure that appropriate decontamination is carried out if hazardous material is spilled onto or into the equipment. They must ensure that no decontamination or cleaning agents are used that could cause a hazard as a result of a reaction with parts of the equipment or with material contained in it. The manufacturer or its agent must be consulted if there is any doubt about the compatibility of decontamination or cleaning agents with parts of the equipment or with material contained in it.

General Precautions

• Always wear laboratory gloves, coats and safety glasses with side shields or goggles.
• Keep your hands away from your mouth, nose and eyes.
• Completely protect any cuts or abrasions before working with potentially infectious materials.
• Wash your hands thoroughly with soap and water after working with any potentially infectious material before leaving the laboratory.
• Remove wristwatches and jewelry before working at the bench.
• Store all infectious or potentially infectious material in unbreakable leak-proof containers.
• Before leaving the laboratory, remove protective clothing.
• Do not use a gloved hand to write, answer the telephone, turn on a light switch, or touch anything that other people may touch without gloves.
• Change gloves frequently. Remove gloves immediately when they are visibly contaminated.
• Do not expose materials that cannot be properly decontaminated to potentially infectious material.
• Upon completion of the operation involving bio-hazardous material, decontaminate the work area with an appropriate disinfectant.

Disposal of Biohazardous Material
The Bigfoot includes waste containers that may contain hazardous biological materials, depending on the sample(s) used. Dispose of the following potentially contaminated materials in accordance with laboratory, local, regional and national regulations:

• Content in waste containers
• Reagents
• Used reaction vessels or other consumables that may be contaminated.

Chemical Hazards
The Bigfoot system includes waste containers and cleaner bottles that may potentially contain hazardous chemical materials, depending on the sample(s) used. Handle and dispose of the materials in accordance with laboratory, local, regional, and national regulations. We recommend the following fluids for cleaning and decontamination.

• Use Attune™ Shutdown Solution (Thermo Fisher Scientific A24975) in the Decon bottle.
• Use an ammonia-free glass cleaner in the Cleaner bottle.
• It is optional to use 10% bleach (0.5-1% sodium hypochlorite) in waste bottles during regular operation.

Prepare the instrument

1. Shutdown the instrument in SQ software.
2. Put on disposable gloves.
3. Use a 3 mm Allen wrench to remove the filter access panel on the left side upper cover.
4. Put paper towels under the filters to prevent fluid from dripping into the bulk fluids compartment below.

Remove and discard used filters
Remove the used filters by following the steps below.

1. Open the Velcro straps that secure the filter you need to remove.
2.  Position a plastic bag, or something similar, around the filter to collect fluid and for easy disposal.
3. Unfasten the black fittings from the top and bottom of the filter. Note: It may be helpful to use the standard black flangeless nut wrench to unfasten the fittings.
4. Discard the used filter and gloves following the lab’s biosafety protocols.

Install new autoclavable filters

Install the new autoclavable filters by following the steps below.

• DI and Sheath – 0.05 µm filters
  Package PL00382 includes two 0.05 µm filters and two sets of luer fittings and are used for DI and SHEATH fluidic lines.

• Decon and Cleaner – 0.2 µm filters
  Package PL00383 includes two 0.2 µm filters and two sets of luer fittings and are used for DECON and CLEANER fluidic lines.

1. Put on a new pair of gloves before handling the replacement filter.

2. Spray the plastic packaging of the luer fittings and the filter with 70% EtOH.

3. Open the bag of luer fittings and spray with 70% EtOH.

4. Spray the black fitting of the exposed outlet fluid line with 70% EtOH.

5. Attach the male luer fitting to the outlet fluid line and ensure that it is properly tightened.

6. Spray the black fitting of the exposed inlet fluid line with 70% EtOH.

7. Attach the female luer fitting to the inlet fluid line and ensure that it is properly tightened. Female luer fitting

8. Open the pre-sterilized filter package and locate small mesh bag of vent/drain cap

9. On a clean surface, carefully open the small mesh bag of vent/drain caps.

10. Spray vent/drain caps with 70% EtOH, install and tighten onto the filter as shown

11. Use a permanent marker to write the today’s date on the new filter body.

12. Pay attention to the blue flow arrow on the filter. The arrow must point down from the inlet to outlet.

13. Spray the male luer fitting from the fluidic outlet with 70% EtOH and attach it securely with your fingers to the filter outlet.

14. Spray the female luer fitting from the fluidic inlet with 70% EtOH and attach it securely with your fingers to the filter inlet.
    1. Fluid inlet linem 2. Filter inlet (female) Filter outlet (male) 4. Fluid outlet line

15. Secure the Velcro strap to keep the filter in place with the blue arrow facing down.

16. Repeat the steps to remove and replace the remaining filters.

Verify filter installation

1. Verify that the 0.20 µm filters are in the Decon and Clean positions.

2.  Verify that the 0.05 µm filters in the DI and Sheath positions.

3. Make sure the filter arrows point down from inlet to outlet.

4. Make sure all Velcro straps are secure.

5. Make sure all fittings and vent/drain caps are tightened securely.

6. Velcro properly secured

7. DI and sheath 0.05 µm filters

8. Decon and clean 0.20 µm filters

9. Fluid outlet line

10. Fluid inlet line

    • Make sure arrows point down

11. Perform instrument Startup in SQ Software.

12. During startup, both the DI and Sheath filter should auto purge air. When complete, there should be a small bubble of air at the top of the filter.

13. Make sure there are no leaks from the DI and Sheath filter connections.

14. Run Quick Shutdown with Decon and Cleaner and verify the Decon and Cleaner filters auto prime during appropriate shutdown steps. It may take more than a minute for fluid to appear in filters and start priming. If there are issues, contact Support. Note: It is okay if the Cleaner and Decon filters do not fill as full as the other filters.

15. When the instrument is ready, run the following steps with a 70 µm tip to verify that there are no leaks from the new fittings and filters. Note: Although any tip can be used, we recommend the 70 µm tip to verify function at high pressure.

16. Shut down the instrument.

17. Start up the instrument.

18. Shut down the instrument.

19. Replace the filter side panel and secure it with 3 mm Allen screw.

20. Start up the instrument and perform QC, Streams setup and Drop Delay. When all processes are successful, the installation procedure is complete.

Technical support – Email cellsorting@thermofisher.com

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