Cole-Parmer SI-200 Series Stuart Refrigerated Shaking Incubator Instruction Manual

: June 13, 2024
: Cole-Parmer

Table of Contents

Cole-Parmer SI-200 Series Stuart Refrigerated Shaking Incubator
PRODUCT OVERVIEW
Instructions for use
- **Introduction
General Description
Operation
Technical Specification
References
Read User Manual Online (PDF format)
Download This Manual (PDF format)

Cole-Parmer SI-200 Series Stuart Refrigerated Shaking Incubator

Cole-Parmer-SI-200-Series-Stuart-Refrigerated-Shaking-Incubator-
product

Cole-Parmer®
SI-200 Series Incubators

Cole-Parmer-SI-200-Series-Stuart-Refrigerated-Shaking-Incubator-
fig-1

PRODUCT OVERVIEW

Cole-Parmer-SI-200-Series-Stuart-Refrigerated-Shaking-Incubator-
fig-2

Cole-Parmer-SI-200-Series-Stuart-Refrigerated-Shaking-Incubator-
fig-3

Microtitre Plate Shaker Incubator SIM-200D-M

Instructions for use

**Introduction

**

Thank you for purchasing this piece of Cole-Parmer equipment. To get the best performance from the equipment and for your own safety, please read these instructions carefully before use. Before discarding the packaging check that all parts are present and correct.

This equipment is designed to operate under the following conditions:

For indoor use only
Use in a well ventilated area
Ambient temperature range +10ºC to +40ºC
Altitude to 2000m
Relative humidity not exceeding 80%
Mains supply fluctuation not exceeding 10%
Over-voltage category II IEC60364-4-443
Pollution degree 2

If the equipment is not used in the manner described in this manual and with accessories other than those recommended by Antylia Scientific, the protection provided may be impaired.

Electrical Installation

THIS EQUIPMENT MUST BE EARTHED

Before connection please ensure that the line supply corresponds to that shown on the rating plate.

Model: SIM-200D-M
Supply requirements: 230V, 50 Hz
Power: 250W

The model is supplied with two mains leads fitted with IEC plugs for connection to the instrument. One has a U.K. 3 pin plug and the other has a 2 pin “Shuko” plug for connection to the mains. Choose the lead appropriate for your electrical installation and discard the other. Should neither lead be suitable, take the lead with the U.K. plug and replace the plug with a suitable alternative. This involves cutting off the moulded plug, preparing the cable and connecting to the re-wireable plug in accordance with its instructions.

THIS OPERATION SHOULD ONLY BE UNDERTAKEN BY A QUALIFIED ELECTRICIAN

NOTE : Refer to the equipment’s rating plate to ensure that the plug and fusing are suitable for the voltage and wattage stated

The wires in the mains cable are coloured as follows:

BROWN : LIVE
BLUE : NEUTRAL
GREEN / YELLOW : EARTH

The instrument is fitted with an IEC socket at the rear of the instrument for connection of the mains lead. The appropriate mains lead should be connected BEFORE connection to the mains supply.

Should the mains lead need replacement, a cable of 1mm2 of harmonised code H05W-F connected to an IEC320 plug should be used.

IF IN DOUBT CONSULT A QUALIFIED ELECTRICIAN

Safety Advice Before Use

Do not attempt to lift or carry the unit alone.
Never move or carry the unit while it is in use or connected to the mains supply
Do not use in a hazardous atmosphere or with hazardous materials.
Ensure that the load is balanced.•Do not attempt to stop movement by hand.
In the case of mains interruption, the shaker function will not restart but the heating function will continue on restoration of the power.
In the case of mechanical interruption (e.g. motor stall), the unit will continue operating on removal of interruption.

Some parts of the incubator may become hot duringuse. These are clearly marked with warning labels and include the heated base and steel parts of the water reservoirs. Avoid touching these parts when the incubator is running.

General Description

The Cole-Parmer SIM-200D-M microtitre plate shaker incubator is ideal for mixing and aerating samples in microtitre plates or micro centrifuge tubes under accurate temperature controlled conditions. High speed combined with a tiny orbit creates the vibrational shaking action required for very small sample volumes.

The retractable platform allows for easy access when loading or unloading samples. This has a non-slip mat to hold microtitre plates in place and micro centrifuge tubes of various sizes can be held in purpose built accessory racks.

The incubator chamber is fitted with two water reservoirs which allow the humidity of the chamber to be increased, thus reducing the possibility of sample loss due to evaporation over longer incubation periods.

Operation

Place the unit on a firm, level, non-slip surface ensuring that there is sufficient free space on all sides without coming into contact with anything during use.
Connect to the electricity supply.

Setting the temperature

Using the top control panel, switch the temperature control unit ON by pressing the control knob.
Hold down the “press to set” button and turn the control knob until the correct temperature is displayed.
Release the “press to set” button. The incubator will then begin to heat to the set temperature. The actual temperature inside the chamber will be displayed on the screen.

Note : The minimum set temperature is 10ºC and the maximum is 60ºC, however the unit will only control to a minimum of 7ºC above the ambient temperature e.g. the minimum temperature at 10 ºC ambient is 17 ºC.

Increasing the chamber humidity
For longer incubations, it may be advantageous to increase the humidity in the chamber to reduce the amount of sample evaporation.

Open the door and slide out, or partially slide out the water reservoirs situated in the top of the chamber.
Fill each reservoir to the ‘max fill’ mark with distilled or deionised This should be sufficient to humidify the chamber to 80-90% humidity for 72h at 37ºC.
Carefully replace the reservoirs in the holders and push back to stop.

Note : When using the water reservoirs for a long period of incubation, it is possible that small quantities of water may be found on the bottom of the chamber. This should not affect the safety or performance of the product but regular drying with a cloth is recommended.

Loading the samples

Lift the release knob on the front of the platform and draw the platform out of the incubator towards
Place the microtitre plates or tube racks on the platform and push down gently onto the non-slip mat to help with

Note: Up to 4 standard or deep-well microtitre plates or two micro tube racks can be accommodated.

Gently push the platform back into the incubator until it locks in place and close the Failure to lock the platform in place before operating the unit can result in damage to the unit.

Setting the speed

Using the keypad at the base of the instrument, switch the unit ON by pressing the control

Note : When using the water reservoirs for a long period of incubation, it is possible that small quantities of water may be found on the bottom of the chamber.

This should not affect the safety or performance of the product but regular drying with a cloth is recommended.

Mode button Start/stop button

Press the mode button until a red dot shows in the display below “rpm”. The display will show the last set
Adjust the speed by turning the control knob. The minimum speed is 250rpm and the maximum speed is
Once the correct speed is displayed, press the start/stop button to begin motion.
The speed can be adjusted at any time without halting the unit by turning the control knob.
To stop the unit at any time, press the start/stop button.

Note : If the door is opened while the unit is in motion a warning alert will sound and the motion will stop. The word “door” will also be momentarily displayed on the screen. The heater will remain ON. Close the door to continue.

Setting the timer

Press the mode button until a red dot shows in the display below “time”. The display will show three horizontal lines or the last set
For continuous operation, set the display to the three horizontal
Select the required countdown time by turning the control knob. The time will be displayed first in minutes and seconds, next in hours and minutes, then in days and hours up to a maximum of 9
If the platform is already in motion, countdown will start immediately. Otherwise, press the start/stop button to begin countdown; motion will also start at the set
During operation, motion and countdown can be paused at any time by pressing the start/ stop Press the start/stop button again to continue.
The timer can be adjusted at any time without halting the unit by turning the control
When the timer reaches zero, the unit will automatically stop motion and an alert will

Note : If the door is opened while the unit is in motion a warning alert will sound and the motion and countdown will stop. The word “door” will also be momentarily displayed on the screen. The heater will remain ON. Close the door to continue and the countdown will resume from the point at which it was stopped.

USB connectivity
The SIM-200D-M has capability to connect to a PC via Tube holder for 1.5ml tubes Tube holder for 0.5ml tubes SSM5/1 SSM5/2

USB for long term tracking of the incubator chamber temperature

First, download the latest version of the SIM-200D-M software from coleparmer.com. Once downloaded, follow the installation instructions.
Remove the waterproof cap from the USB port on the side of the SIM-200D-M and connect the included
Connect the other end of the cable to a free USB port on a
Follow the software instructions for new hardware installation.
Click ‘Run Software’ to complete the program start-

Maintenance & Servicing

WARNING: Ensure the unit is disconnected from the mains electricity supply before attempting maintenance or servicing. Periodically clean the instrument using a damp cloth and mild detergent solution. Do not use harsh or abrasive cleaning agents. Any repairs or replacement of parts MUST be undertaken by suitably qualified personnel. For a comprehensive list of parts required by service engineers conducting internal repairs, please contact the Service Department, quoting both the model number and serial number:

E – mail : cpservice@antylia.com
Tel : +44 (0)1785 810475

Only spare parts supplied or specified by Antylia Scientific or its agents should be used. Fitting of non- approved parts may affect the performance of the safety features designed into the instrument. If in any doubt, please contact the Service Department of Antylia Scientific Ltd. or the point of sale.

For any other technical enquiries, please contact the Technical Support Department:

E – mail : cptechsupport@antylia.com
Tel : +44 (0)1785 810433.

Spares and Accessories

Tube holder for 1.5ml tubes: SSM5/1
Tube holder for 0.5ml tubes: SSM5/2
Tube holder for 0.2ml tubes: SSM5/3

Technical Specification

SIM-200D-M

Temperature range : Ambient +7ºC to 60ºC 0.1ºC
Temperature display resolution : 0.5ºC
Temperature fluctuation Speed range : ±0.5ºC at 37ºC
Orbit diameter : 250 to 1250rpm
Maximum load : 1.5mm
Speed control : 1kg
increments : Digital set in 10rpm
Timer : min/s, h/min, day/h
Electrical supply : 230V, 50Hz, 250W
Fuses : 2 x Anti-surge T3.15A
Dimensions (w x d x h) : 361 x 405 x 430mm
Net weight : 22.5kg

This product meets the applicable CE Directives and UKCA Legislation for radio frequency interference and may be expected not to interfere with, or be affected by, other equipment with similar qualiﬁcations. We cannot be sure that other equipment used in its vicinity will meet these standards and so we cannot guarantee that interference will not occur in practise. Where there is a possibility that injury, damage or loss might occur if equipment malfunctions due to radio frequency interference, or for general advise before use, contact the manufacturer.

Declaration of Conformity is available to view online at www.coleparmer.com

EU Representative address
Antylia Scientific GmbH Futtererstraße 16 97877 Wertheim Deutschland