MEGACOR BMBT-Vet FASTest FIV Single Test Instruction Manual

MEGACOR BMBT-Vet FASTest FIV Single Test

INFORMATION ON THE TEST-KIT

TEST-KIT COMPONENTS

Test-kit BMBT-Vet. Dog contains:

• 10 disposable incision lancets (W×D 3.0 × 1.0 mm)
• 10 fi lter papers
• 1 instructions for use

Test-kit BMBT-Vet. Cat contains:

• 10 disposable incision lancets (W×D 2.2 × 0.6 mm)
• 10 fi lter papers
• 1 instructions for use

STABILITY AND STORAGE

APPLICATION

LIABILITY
The entire risk due to the performance of this product is assumed by the purchaser. The manufacturer shall not be liable for indirect, special or consequential damages of any kind resulting from the use of this product.

INTRODUCTION

Hemostasis is a complex physiological reaction on bleedings of all kind. Hemostasis disorders can base on primary and secondary defects or causes. The buccal mucosal bleeding time (BMBT) is the time that passes by after placement of a defi ned incision by means of BMBT-Vet. Dog or Cat, respectively, until hemostasis occurs. It is an important coagulation marker for validation of the individual primary state of hemostasis. With help of BMBT, suspicion of disorders of the primary hemostasis can be confi rmed quickly and simply. Characteristics normally are surface bleedings, but also chirurgical reasons, especially in cats. They are caused by infectious and / or immunological reasoned thrombocytopenia, thrombocytopathies, vasculopathies and the genetically inherited von Willebrand disease (vWD) type 1–3. With help of BMBT-Vet. Dog or Cat, respectively, by a standardised depth and length of the cut, the veterinarian is enabled on-site to obtain constant and reproducible BMBT results and to give a specifi c statement about the status of hemostasis in the dog and cat.

PATIENT PREPARATION

• a. DOG
  • Optimally, bring the patient into lateral position.
  • Uncover the mucosal side turning up carefully the upper chaps. Fix the turned chaps carefully with a gauze bandage around the upper jaw (just enough to prevent the venous return partially). b. CAT
  • Optimally, bring the patient into lateral position. With uncooperative animals, a sedation is recommended. When choosing an anaesthetic, care should be taken to ensure that no drugs lowering the blood pressure are used, as these may affect the bleeding time.
  • Uncover the mucosal side turning up carefully the upper chaps. Fix the turned chaps carefully with a gauze bandage around the upper jaw (just enough to prevent the venous return partially).

TEST PREPARATION

• a. Remove the BMBT-Vet. Dog or Cat, respectively, from its sterile foil pouch immediately before use. Take care that the sterile blade slot surface will not be contaminated by touching (fi g.1).
• b. Remove the safety clip (fi g.2). This can be replaced in its position in case of test start delay for a short period of time.

TEST PROCEDURE

1. Search a healthy and secure incision place. Be aware not to damage bigger blood vessels (e. g. Arteria labialis).
2. Place the BMBT-Vet. Dog or Cat with the entire contact surface lightly on the mucosa surface. Push the trigger (fi g.3). Immediately remove the BMBT-Vet. Dog or Cat and start the stop watch simultaneously with start of bleeding (fi g.4).
3. Let the incision site bleed freely and undisturbed (fi g.5). Take care that no blood fl ows into the mouth or that the patient licks the site with its tongue.
4. Absorb the draining blood every 10 seconds with the fi lter paper without touching the bleeding site (fi g.6).
5. Measure the time until the bleeding stands.

READING OF THE TEST RESULT

• Stop the stop-watch as soon as the bleeding stands and read the BMBT (fi g.7).

PRECAUTIONS FOR USERS

• For single use only!
• The guidelines for working in medical laboratories must be observed. It is recommended to wear disposable gloves and other personal protective equipment (protective clothing, possibly a face mask). Wash and disinfect hands after completing the test.
• The BMBT-Vet. Dog or Cat must be seen as potentially infectious after use and disposed of accordingly, together with the used test-kit components.

TEST PRINCIPLE

The determination of the BMBT is a useful screening test in the veterinary clinic for the identification of thrombolytic and vascular bleeding disorders. The incision using the BMBT-Vet. Dog or Cat only leads to a superficial capillary bleeding. With the BMBT-Vet. Dog or Cat, with the help of an easy- to-handle, automatical triggered chirurgical blade (incision lancet), a defined cut depth of 1.0 mm (dog) or 0.6 mm (cat) and a cut width of 3.0 mm (dog) or 2.2 mm (cat) is set quickly (1/1000 s) and painless. After planar placing on the mucosa and manual release (steel spring), a precise cut in the mucosa of the patient is set. Due to automatic retraction of the steel blade after setting the incision, there is no possibility of injuries neither for the patient nor for the veterinarian. The duration of the bleeding depends on the formation rate and stability of the thrombus and therefore is mainly based on the number and function of available platelets. The plasmatic coagulation system is not activated, the coagulation factors are not needed to stop this kind of bleeding. Standardized incision depth and length enable the veterinarian to produce constant and repeatable measurements under practice conditions.

INFORMATION FOR THE INTERPRETATION

Physiological values:

• dog: < 4 minutes (reference range: 0.75–2.25 min)
• cat: < 1.5 minutes

Prolonged values:

• Von Willebrand disease
• Thrombocytopathy
  • inherited
  • acquired (e.g. aspirin)
• Thrombocytopenia (< 100,000 / μl)
  • immune-mediated
  • infection mediated (e. g. ehrlichiosis)
  • bone marrow diseases
• Vascular disease
• Uraemia

Standardised screening test for the detection of primary hemostasis disorders in the dog and cat

CONTACT

• Supplied Exclusively To The UK
• Veterinary Market By
• Vetlab Supplies Ltd
• Visit Our Website
• www.vetlabsupplies.co.uk.
• Telephone: 01798 874567
• email us: info@vetlabsupplies.co.uk.

Manufacturer:

• 6912 Hörbranz – AUSTRIA
• www.megacor.com.

References

• MEGACOR Diagnostik GmbH – Veterinary in vitro diagnostics
• Wholesale Vet Supplies & Products for Practices & Labs | Vet Supplies Online UK

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