VistaLab L065-0001-001 Ovation Macro Pipettes User Guide

L065-0001-001 Ovation Macro Pipettes

Product Description & Specifications

The Ovation BioNaturalTM electronic single channel and macro
volume pipettes are designed to handle repetitive and complex
liquid handling tasks that contribute to repetitive stress injuries
in the laboratory. They increase laboratory efficiency through
automated liquid handling routines, while reducing fatigue and the
effect of poor postures. The pipettes are adjustable volume, air
displacement, fully electronic motor-driven pipettes intended to
aspirate and dispense precise fluid volumes.

The Ovation BioNaturalTM pipettes come in various colors and
dispense increments:

*Specifications subject to change.

Product Usage Instructions

Pipetting:

1. Press the tip eject button to remove the tip ejector
   sleeve.

2. Select the liquid handling function by pressing the
   corresponding button on the keypad (aspirate, dispense, or
   blowout).

3. To acquire tips, insert the pipette into a container of tips
   and press down on the pipetting trigger. Release the trigger to
   pick up a tip.

4. To aspirate, immerse the tip into the liquid and press down on
   the pipetting trigger to the first stop. Hold for one second and
   release.

5. To dispense, touch the tip to the side of the receiving vessel
   and press down on the pipetting trigger to the second stop. Hold
   for one second and release.

6. To eject tips, press the tip eject button or ejector
   sleeve.

Calibration:

1. Press and hold the volume label and power buttons
   simultaneously to enter calibration mode.

2. For a new calibration factor, enter the value using the keypad
   and press enter. For a known calibration factor, enter the value
   and press and hold the aspirate button until the pipette
   beeps.

3. For calibration with serological or volumetric pipettes, follow
   the instructions in Appendix A of the operator’s guide.

Maintenance & Cleaning:

1. Clean the exterior of the pipette with a mild detergent and
   water or alcohol-based solution.

2. To disinfect nozzles, immerse in a 70% ethanol solution for two
   minutes and air dry.

3. To replace a nozzle or nozzle filter, follow the instructions
   in Appendix A of the operator’s guide.

For more information on safety compliance and warranty, refer to
Appendix A of the operator’s guide. Tips and accessories are listed
in Appendix B.

Operator’s Guide for Electronic Single Channel and Macro Volume Pipettes

L065-0001-001 Rev. F

0.2-10mL

TABLE OF CONTENTS

Introduction

Product Description & Specifications

1-1

Pipette Components

1-2

BioNatural

Introduction & Ergonomics

2-1

Pipetting

Ovation pipetting

2-3

Picking Up & Holding

2-3

Selecting Liquid Handling Functions

2-3

Acquiring Tips

2-3

Aspirating and Dispensing

2-4

Ejecting Tips

2-4

Using serological or volumetric pipettes (macro models)

2-5

Operating

Getting Started, Keypad & Display

3-1

Procedures

Function Indicators and Setting Indicators

3-2

Set-Up of Liquid Handling Functions

3-2

Set-Up of Pipette Settings

3-9

Calibration

Introduction

4-1

Determining a New Calibration Factor

4-1

Entering a Known Calibration Factor

4-2

Calibration with Serological or Volumetric pipets

4-2

Maintenance &

Cleaning Exterior

5-1

Troubleshooting

Disinfecting Nozzles

5-1

Replacing a Nozzle (Single Channel)

5-1

Replacing a Nozzle (Macro 5mL and 10mL)

5-2

Replacing a Nozzle Filter (Single Channel)

5-3

Replacing a Nozzle Filter (Macro 5mL)

5-4

Replacing a Nozzle Filter (Macro 10mL)

5-5

Replacing a Nozzle Insert (Macro 10mL)

5-5

Battery Information

5-6

Troubleshooting

5-7

Appendix A

Warranty, Safety Compliance & Contact Information

A-1

Hazards & Precautions

A-2

Operating Temperature & Environment Conditions

A-2

Power Supply and Battery Specifications

A-2

Chemical Compatibility

A-2

Appendix B

Tips & Accessories

B-1

Ovation BioNatural Pipette ­ Electronic Single Channel Operator’s Guide

ELECTRONIC SINGLE CHANNEL AND MACRO OVATION PIPETTES

1

Product Description

Ovation BioNaturalTM pipettes are the ideal ergonomic solution for handling repetitive and complex liquid handling tasks that contribute to repetitive stress injuries in today’s laboratory. Ovation pipettes increase laboratory efficiency through automated liquid handling routines, while reducing fatigue and the effect of poor postures.
This manual describes how to use and care for your Ovation electronic single channel or macro pipette. As you can see from its appearance, it is different from every pipette that you have used before, therefore please read the instructions carefully.
Upon initial receipt of the pipette, the battery must be charged for 90 minutes prior to use. (See instructions on page 5­5). Also, please activate the warranty on the VistaLab Technologies web site ­ www.vistalab.com. The required on-line form can be found in the “support” menu. Retain all packing materials in the event that the pipette is to be sent back to VistaLab Technologies for calibration verification or service.
Electronic single channel and macro Ovation BioNatural Pipettes are adjustable volume, air displacement, fully electronic motor-driven pipettes intended to aspirate and dispense precise fluid volumes.

Catalog#
1065-0020 1065-0020L
1065-0125 1065-0125L

Color Yellow Green

Volume Range 0.5 ­ 20µL
2 ­ 125µL

Dispensing Increments
0.05µL

Accuracy*
±1.0% at 20µL ±1.5% at 10µL ±3.0% at 2µL

0.5µL

±0.8% at 125µL ±0.8% at 62.5µL ±2.9% at 12.5µL

Precision*
0.30% at 20µL 0.50% at 10µL 1.80% at 2µL
0.15% at 125µL 0.24% at 62.5µL 1.00% at 12.5µL

1065-0250 1065-0250L

±0.8% at 250µL

0.15% at 250µL

5 ­ 250µL

1µL ±0.8% at 125µL

0.25% at 125µL

Blue

±2.5% at 25µL

1.00% at 25µL

1065-1250 1065-1250L

Purple

25 ­ 1250µL

±0.75% at 1250µL 5µL ±0.8% at 625µL
±2.8% at 125µL

0.15% at 1250µL 0.20% at 625µL 0.60% at 125µL

1065-05mL 1065-05mLL

±0.6% at 5000µL

100 ­ 5000µL 10µL ±0.6% at 2500µL

Grey

±2.4% at 500µL

0.16% at 5000µL 0.20% at 2500µL 0.60% at 500µL

1065-10mL 1065-10mLL

±0.6% at 10mL

0.2 ­ 10mL

10µL ±1.0% at 5mL

Red

±2.4% at 1mL

0.16% at 10mL 0.20% at 5mL 0.30% at 1mL

Note: Catalog numbers ending in “L” are left-handed models ­ the LCD screen is on the opposite side of the pipette.

*Specifications subject to change

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

1 – 1

Pipette Components
100-5000µL 0.2-10mL

Tip Eject Button Ejector Sleeve

Pipetting Trigger

Adjustable Hook

Nozzle Keypad

Body LCD Display

Recharging Port

Volume Label

Captive Screw

Serial Number

Description Pipetting Trigger Tip Eject Button Adjustable Hook Ejector Sleeve Nozzle Body Keypad LCD Battery Volume Label Recharging Port Power Supply Captive Screw Serial Number

Function Initiates pipetting action Ejects disposable pipette tips with minimal thumb force Accommodates left and right handed users Stored energy from tip installation releases tips Tapered, chemically resistant coupling that secure pipette tips Larger grip surface for comfort, eliminating tight hand grip Selects and sets up liquid handling functions, settings and calibration Indicates liquid handling functions, status, settings, and remaining battery power Lithium ion rechargeable, located in base of pipette (not shown) Identifies volume range Power supply connection for battery recharging AC adapter for recharging battery (not shown) When loosened, allows access to inside of pipette for battery replacement Provides positive identification for the pipette

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

1 – 2

Introduction Posture

BIONATURAL PIPETTING 2
Studies have shown that pipetting is the #1 cause of musculoskeletal disorders in the laboratory ­ it is, by nature, a repetitive process that puts strain on the body. While we can’t change how repetitive pipetting is, we can and did change how comfortable you are while doing it.
The Ovation BioNatural Pipette is the only pipette designed to keep your hand in the neutral position recommended by ergonomics experts. We call this BioNaturalTM pipetting ­ it allows a lower hand location to ease stress in the shoulder, and a relaxed wrist angle eliminates uncomfortable extension and radial deviation movements in the arm. Force, velocity and exertion from repetition or duration have also been neutralized because of the Ovation pipette’s unique working position and reduced forces required during operation.
Some practice may be required to change years of posture and habits developed using standard axial pipettes; the physical benefits of BioNatural pipetting are worth the practice! When using the Ovation pipette, arm/hand elevation should remain low to the work surface, wrist rotation should not exceed 90º, and hand posture should remain relaxed with the wrist and back of hand slightly flexed.
Arm elevation remains low, minimizing stress to elbow, shoulder and neck

A loose, relaxed grip increases available strength in the hand, improving endurance and productivity during pipetting

Wrist remains in a neutral range of motion throughout all pipetting operations

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

2 – 1

Keep a “comfort zone” for pipetting

“Comfort Zone”

Maximum Reach

14″ 24″

14″ 24″

Ergonomics experts recommend establishing a “comfort zone” of movement for the task of pipetting. Position your body within 9″ of the counter surface, and keep the items needed within easy reach. Ideally this should be 14″ or less, and should not exceed 24″ of occasional reaching. Wrist rotation should not exceed 90° from the work surface, and arm/hand elevations should remain low to the counter surface.

1. Relaxed shoulders and upper arms
2. Low thumb forces 3. Relaxed hand grip
4. Neutral forearm and wrist rotation
5. Low arm and elbow height
Ovation comfort-zone benefits: 1. Minimized tension and fatigue 2. Minimized exertion and contact stress 3. Low contact pressure 4. Lowest pressure in carpal tunnel and to median nerve 5. Minimized tension to shoulder and elbow

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

2 – 2

Picking Up the Pipette
Selecting Liquid Handling Functions and Changing Settings
Acquiring Tips

The Ovation pipette has been designed to allow the body of the pipette to fill your palm. Rotate the adjustable hook to rest comfortably on your forefinger. The texture of the unit’s back helps reinforce correct hand positioning.
The unique ergonomic design and adjustable hook is compatible for both right and left-handed use.
Keep hand posture relaxed ­ there’s no need for a “firm grip” when using an Ovation pipette.
The Ovation pipette’s keypad is easy to use and provides access to all liquid handling functions and settings. Simply press the buttons on the keypad, monitoring the selection of functions or changes to settings on the LCD display.
Complete information and examples of all liquid handling functions and settings are included in “Operating Procedures” – Chapter 3 of this guide.
While maintaining a flat wrist-hand posture, insert the Ovation pipette nozzles into a tip. Press down until you hear or feel a “click”. This “click” indicates that the tip is properly seated and ready for use.

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

2 – 3

Aspirating and Dispensing

Smooth gliding movements of the upper extremities with proper posture and minimal stress is the key to ergonomically correct pipetting. Maintain a low forearm elevation and neutral wrist posture throughout the pipetting cycle. With Electronic Single Channel models, Immerse pipette tip to an appropriate depth (1mm
for 0.5­20µL, 2 to 3mm for 2µL­125µL & 5250µL, and 2 to 4mm for 25­1250µL). Press & release the pipetting trigger to aspirate sample. Press pipetting trigger again to dispense or mix sample.

Ejecting Tips
Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

Aspirating and dispensing large sample volumes traditionally create ergonomic challenges because of an axial pipette’s design, elongated macro tips, or the overall length of typical serological pipets.
The Ovation macro pipette allows the operator to minimize wrist rotation and arm elevation.
Immerse pipette tip into sample, then press and release the pipetting trigger to aspirate. Wait one second after beep tone, then remove tip and place it into dispensing container at same angle used during aspiration. Press pipetting trigger again to dispense or mix sample.
Note: To interrupt aspirating cycle and/or prevent accidental overfilling of a pipet (as when set volume exceeds capacity of serological pipet), quickly press and release pipetting trigger. PURGE will be on display. Pressing pipetting trigger again then expels any liquid or air bubbles in the tip or pipet.
Ovation pipettes store energy captured during the acquisition of a tip, and use it to discard tip when the tip eject button is pressed. Point pipette tip into a suitable waste container and press the tip eject button to discard the tip.
To minimize forearm elevation and “reaching”, position the waste container below worksurface level or use shallow containers. Raising the arm to discard tips into a tall receptical on the counter may negate some of the benefits of Ovation’s low-profile design.
2 – 4

Using serological or volumetric pipets
(macro models only)
Note: Due to the additional length of the air column or variations in markings on serological or volumetric pipets, it may be necessary to adjust the Ovation’s calibration factor. To optimize performance, review the calibration instructions in chapter 4.

When extra length is needed to reach the bottom of tall vessels, glass or plastic serological or volumetric pipets can be used with Ovation macro volume models.
The nozzle on the 0.2-10mL model accommodates both tips and pipets. Insert the end of a pipet into the nozzle until it stops. To remove the pipet, manually pull it out of the nozzle.
The 100-5000mL model requires installation of a Sero-Adapter prior to use with serological pipets. The adapter easily attaches to the pipette in place of its standard nozzle (See instructions below). Insert the end of a pipet into the Sero-Adapter until it stops. To remove the pipet, manually pull it out of the Sero-Adapter. Re-install the standard nozzle to use pipette tips.

Installing a Sero-Adapter (100-5000µL model only)

1. Place a macro pipette tip onto the nozzle and push on ejector sleeve until click is heard. Grip tip firmly near the nozzle, and turn it in a counterclockwise direction to unscrew and remove nozzle from pipette. Store nozzle in a safe place.

2. Carefully screw on adapter (clockwise). Ensure adapter is square to threads for proper installation.

Nozzle

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

2 – 5

OPERATING PROCEDURES 3

Getting Started

The general steps to set up and operate an electronic single channel or macro model Ovation pipette are: 1. Select a liquid handling function 2. Review and/or change settings as required 3. Acquire tip 4. Run the liquid handling function according to its protocol 5. Eject disposable tip into a proper waste container.
Familiarize yourself with the use of the keypad, display prompts, set-up and use of the pipette’s liquid handling functions in this chapter before use.

Pipette Keypad

Button UP DOWN

Description Advances display to next liquid handling function in the run mode. When in set-up, the button advances display to the next setting within the liquid handling function that can be changed.
When changing the setting(s) within a liquid handling function, pressing the or button adjusts the settings. When pressed together and held, they are used to access pipette settings.

Pipette Display
example

Description
Displays volume and values during set-up, e.g. # of mix cycles, # of multiple dispenses

Symbol indicates next portion of aspirate (up) or dispense (down) cycle to be performed

Displayed when cal factor has been changed from factory setting of 1.000

Icon indicates remaining battery capacity

Displays liquid handling function, setting or prompt that is active

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

3 – 1

Function Indicator p1 p2 p3
PDB
(no function shown)
multi
sdilu
mix rever
(not available on Macro models)
Setting Indicator FILL

Function Pipette

Description
Routine aspirating and dispensing at stored (user-defined) volumes (also known as TC or “to contain” pipetting). Sample volume is dispensed with blow-out to expel all liquid.
(P3 not available on macro models))

Pipette with Delayed Blowout

Routine aspirating and dispensing at a stored (user-defined) volume (“to contain” pipetting) with a delayed blowout. This is especially useful when using viscous liquids or serological pipets. (Available on macro models only)

Pipette

Aspirating and dispensing in pipette function at a dynamic user-defined volume.

Multiple Dispense

Repetitive dispensing function of equal volumes. The pipette calculates and aspirates the total volume required, then dispenses in multiple aliquots. An automatic repetitive dispensing utility, PACE, can be activated by holding the pipette trigger while dispensing.

Serial Dilute

A sequence of dilutions. Sample is aspirated, dispensed into diluent and mixed. Diluted sample is automatically aspirated for addition to the next diluent.

Mix

Repetitive cycles of aspirating and dispensing.

Reverse Pipette

A sample transfer function based on delivered dispense volume (also known as TD or “to deliver” pipetting). Sample volume is aspirated with slight overage and the desired volume is dispensed, leaving some liquid in the tip that is then purged.

Description
Volume to be dispensed (when using p1, p2, p3 or PDB, this is also the volume that will be aspirated).

Speed

Speed at which the pipette aspirates, dispenses and mixes within a liquid handling function. 5 settings are available. (1 is the slowest and 5 is the fastest)

Dis

Shows the number of dispenses remaining when using the

MULTI function.

Disp

The volume of individual dispense(s) when setting up the MULTI function.

Mix

The volume of the dilution to be mixed when using the

SDILU function.

Vol

The volume to be mixed when using the MIX function.

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

3 – 2

Setting Indicator Count Purge Zero Pace Tone
LCD Sleep HOME

Description
The number of dispenses when using the MULTI function, or the number of mix cycles (1-9) when using SDILU or MIX functions.
Last step in some Liquid Handling Functions, or is displayed when a function has been interrupted.
Indicates that the pipette pistons are at the blow-out or lowest position at the end of a MIX function.
Pace is an adjustable timed interval delay between automatic repetitive dispenses when using the MULTI function (1 is slowest setting, 4 is fastest).
Ovation pipettes use “beep” tones to indicate various actions, or can be disabled.
· A single beep tone indicates the end of a pipetting step.
· A double beep tone indicates the completion of the (last) dispense cycle when using the MULTI function and REVER .
· A triple beep tone indicates the end of a liquid handling function.
· An alert tone indicates the pipetting trigger was pressed before set-up was completed, or an illegal action or programming error has occurred.
The contrast on the liquid crystal display can be adjusted as needed.
After 10 minutes of inactivity, the pipette automatically enters SLEEP to conserve battery power. Press any button or the pipetting trigger to resume operation.
When the pipette is first activated from SLEEP, the HOME prompt is displayed while the plungers are automatically reset.

Set-up of Liquid Handling Functions

Introduction

The Ovation pipette’s keypad is easy to use and provides access to all liquid handling functions and settings. Simply press the buttons on the keypad to step through the various functionss and make changes to settings. When a setting indicator is flashing, this indicates that the setting has been changed from a previously “saved” setting.
During setup, settings such as speed, count, and mix may appear within a liquid handling function. While their set-up is similar in all cases, they must be established independently within each liquid handling function.

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

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7

7

Pipette
10 0.05µL
P1
10 0.0µL
FILL
50 0.0µL
Fill
.2
Speed
4
SPEED
50 0.05µL
P1
50 0.06µL
P1

Liquid Handling Functions
Ovation pipettes provide user-defined pre-sets for routine pipetting (TC or “to contain”) at exact volumes with automatic blow-out (P1, P2 & P3 on electronic single channel models, P1 & P2 on macro models). An additional pipetting function (PDB) with a delayed blow-out is available on macro models which may be desirable when using viscous liquids or a serological pipet. Volume and speed settings are stored for easy recall and use.
To set-up and run a P1, P2, P3 or PDB liquid handling function, follow these steps:
Example: Pipette 500µL of sample
1. Press and release until P1, P2, P3 or PDB appears on the display.
2. Press or and the FILL appears on the display with the current volume setting. Press and hold or to rapidly scroll to the desired setting, or press and release or to step the volume measurement to the desired setting.
3. Press and release to lock-in the new volume setting and advance to the current Speed setting.
4. Press and release or until the desired speed is displayed. (1 is slowest setting, 5 is fastest).
5. Press and release to lock-in the Speed setting and exit setup.
6. Aspirate sample by pressing and releasing the pipetting trigger.
7. Press the pipetting trigger to dispense sample. When using P1, P2 or P3, blow-out is performed automatically to expel all liquid.
! To prevent accidental re-aspiration of sample after blow-out, the pipetting trigger can be held down during dispensing. Move tip away from the dispensing area before releasing the pipetting trigger.
If using the PDB function (macro models only), press the pipetting trigger again to initiate blow-out. This operator-controlled delayed blow-out may be desired when using viscous liquids or a serological pipette.

2 0 0.05µL

Setting a dynamic volume
To aspirate and dispense any volume, it is not necessary to store the new volume setting. Simply press and release until P1, P2, P3 or PDB appears on the display, then press or until the desired volume is displayed. Press and or to change speed if desired. Press and release the pipetting trigger to begin pipetting. Note: P1, P2 or P3 will not appear on the display. This volume will remain active until another liquid handling function or volume is selected; pre-set volumes for P1, P2 , P3 or PDB remain unchanged.

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

3 – 4

Multiple Dispense

Multiple Dispense is a repetitive pipetting function for dispensing equal volumes. Once a dispense volume and the number of dispenses are established, the Ovation pipette automatically calculates the total fill volume required.
To set-up and run a Multiple Dispense liquid handling function, follow these steps:

7

7

8505µL
m u l ti
25 µL
DISP
50 µL
DISP
.5
COUNT
10
COUNT
.2
Speed
5
SPEED
5005µL
Fill

Example: Dispense a 50µL aliquot, 10 times
1. Press and release until MULTI appears on the display.
2. Press or and DISP appears on the display with the current volume setting for individual dispenses. Press or until the desired volume is displayed.
3. Press and release to lock-in the new volume setting and advance to the current COUNT setting. Note: The count setting will either be the last number of dispenses used or the maximum number of full dispenses that can be performed at the new volume setting.
4. Press and release or until the desired number of dispenses is displayed.
5. Press and release to lock-in the new COUNT setting and advance to the current speed setting.
6. Press and release or until the desired speed is displayed (1 is slowest speed, 5 is fastest).
7. Press and release to lock-in the Speed setting and exit setup.
8. Aspirate sample by pressing and releasing the pipetting trigger. The total volume required to complete all dispenses and FILL is displayed while the volume is being aspirated.

77

506µL
DIS 10
506µL
DIS 9

9. Press and release the pipetting trigger to dispense each aliquot. The display will indicate the volume being dispensed, and the number of dispenses remaining.
To activate Pace , press and hold the pipetting trigger when dispensing, and aliquots will be dispensed automatically at a timed interval until the pipetting trigger is released or the last dispense is performed. See the Pace instructions to set-up or change the interval setting (page 3-9).

PURGE

10. After the final volume is dispensed, purge appears on the display. Press and release the pipetting trigger to discard any remaining liquid in the tip.
Note: purge can be used at any time to end any liquid handling function by pressing . To cancel purge, press again and resume dispensing.

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

3 – 5

7

7

Serial Dilute
505µL
SDILU
50 µL
FILL
10 05µL
FILL
75 µL
MIX
125 µL
MIX

The SDILU liquid handling function performs sequential dilutions. An initial sample volume is aspirated, dispensed into diluent and then mixed. The diluted sample is then aspirated for addition to the next diluent. To set-up and run a Serial Dilute liquid handling function, follow these steps: Example: Prepare a 1:2, 1:4, ,1:8 . . . serial dilution using a sample volume of
100µL. Mix 125µL of each dilution three times. 1. Use the MULTI liquid handling function to dispense 100µL of diluent into each
sample well.
2. Press and release until SDILU appears on the display.
3. Press or and FILL appears on the display with the current volume setting for aspiration. Press or until the desired setting is displayed.
4. Press and release to lock-in the new volume setting and advance to the current MIX volume.
5. Press or until the desired mix volume is displayed.
6. Press and release to lock-in the new mix volume and advance to the current COUNT setting.

.5
COUNT

7. Press or until the desired number of mix cycles (1-9) is displayed.

7

3
COUNT
.2
Speed
4
SPEED

8. Press and release to lock-in the new COUNT setting and advance to the current speed setting.
9. Press and release or until the desired speed is displayed (1 is slowest, 5 is fastest).
10. Press and release to lock-in the Speed setting and exit setup.

7

10 05µL
SDILu

11. Aspirate 100µL of sample by pressing and releasing the pipetting trigger.
12. Place (filled) tip into the first diluent-filled row, and press and release the pipetting trigger to dispense sample into the diluent, followed by three mix cycles. After mixing, 100µL of the diluted sample is automatically aspirated. Move tip to next row and press pipetting trigger to dispense and mix the next dilution. Continue until all dilutions are prepared.

13. To empty the tip of liquid after the final dilution is made, press and purge appears on the display (or to cancel PURGE, press again). Press and release the pipetting trigger to dispense liquid into an appropriate container.

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

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7

7

7

7

Mix
50.05µL
MIX
50.0 µL
VOL
10 0.0µL
VOL
.3
COUNT
5
COUNT
2
Speed
4
SPEED
100.05µL
MIX 5
ZERO
100.05µL
MIX 5

Mix is a liquid handling function with repetitive cycles of aspirating and dispensing of the same sample. The Mix function can be used independently of other functions and is included within the SDILU function. To set-up and run the Mix liquid handling function, follow these steps: Example: Mix 100µL of solution 5 times
1. Press and release until MIX appears on the display.
2. Press or and VOL appears on the display with the current volume setting for mixing. Press or to scroll to the desired setting.
3. Press and release to lock-in the new volume setting and advance to the current Count setting.
4. Press and release or until the desired number of mix cycles is displayed (1-9).
5. Press and release to lock-in the new COUNT setting and advance to the current SPEED setting.
6. Press and release or until the desired speed is displayed (1 is slowest, 5 is fastest). 7. Press and release to lock-in the Speed setting and exit setup.
8. Mix 100µL of sample by pressing and releasing the pipetting trigger.
9. When zero appears on the display, remove tips from solution and press the pipetting trigger. The pipette is now ready to perform the next Mix function.

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

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7

7

7

Reverse Pipette
(Not available on Macro models)
10 0.05µL
REVER
10 0.0µL
DISP
50.0 µL
DISP
.2
Speed
4
SPEED
50.05µL
REVER
PURGE
505µL
Rever

Reverse Pipette is a sample transfer function based on an exact dispense volume (TD or “to deliver” pipetting). Sample volume is apirated with a slight overage and the exact amount is dispensed, leaving some liquid in the tip that is purged with the next press of the pipetting trigger. This method of pipetting is commonly used for highly volatile or viscous samples, and/or when working with small microvolumes.
To set-up and run a Reverse Pipette liquid handling function, follow these steps: Example: Pipette 50µL of sample
1. Press and release until REVER appears on the display.
2. Press or and DISP appears on the display with current volume setting. Press or tuntil the desired setting is displayed.
3. Press and release to lock-in the new volume setting and advance to the current Speed setting.
4. Press and release or until the desired speed is displayed (1 is slowest, 5 is fastest).
5. Press and release to lock-in the Speed setting and exit setup.
6. Aspirate sample by pressing and releasing the pipetting trigger.
7. Press and release the pipetting trigger to dispense sample.
8. The PURGE prompt is displayed after sample is dispensed. Press and release the pipetting trigger to purge liquid remaining in the tip into a suitable container. The pipette is now ready to perform the next Reverse Pipette function.

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

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7

7

Tone
on
tone
off
Tone
LCD
on
tone
IIII
LCD +-
IIII
LCD +-

Set-up of Pipette Settings

Ovation pipettes use “beep” tones to indicate various actions. A single beep tone indicates the end of a pipetting step. A double beep tone indicates the completion of the (last) dispense cycle when using the multiple dispense or reverse pipette function. A triple beep tone indicates the end of a liquid handling function. An alert tone indicates the pipetting trigger was pressed before set-up was completed or an illegal action or programming error has occurred.

Beep tones can be disabled if desired as follows:

1. Simultaneously press and hold the

buttons until TONE appears on the display.

2. Press or to toggle the “beep” setting ON or OFF. Press to step to LCD or press the pipetting trigger to return to routine operation.

The contrast on the pipette’s liquid crystal display can be adjusted as needed. Note: disconnecting battery returns LCD to a default intensity.

1. Simultaneously press and hold the

buttons until TONE appears on the display.

2. Press and release until LCD appears on the display.

3. Press or to increase or decrease the contrast to desired level. Press to step to PACE or press the pipetting trigger to return to routine operation.

Pace
on
Tone
2
PACE

The Pace setting represents the pause time between automatic repetitive dispenses while using the Multiple Dispense function.

To change PACE setting, follow the steps below:

1. Simultaneously press and hold the

buttons until TONE appears on the display.

2. Press and release until PACE and a numeric value is displayed (1, 2, 3 or 4). Press the or button until the desired pause interval is displayed (1 is slowest, 4 is fastest). The lower the number, the shorter the delay in milliseconds between automatic dispenses.

3. Press and release to step to CAL or press the pipetting trigger to return to routine operation.

Note: To use PACE when in the Multiple Dispense liquid handling function, press and hold the pipetting trigger when dispensing; aliquots will be dispensed automatically at the specified interval setting. To interrupt PACE, release the pipetting trigger.

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

3 – 9

Introduction
Determining a New Calibration Factor

CALIBRATION 4

Each Ovation pipette is factory calibrated to manufacturing specifications and a Certificate of Calibrtation traceable to NIST is enclosed in the unit’s original packaging. The pipette is calibrated at 21.5°C ( ± 2°) and relative humidity of 45%-75% using distilled water. It is recommended that calibration be verified every six months or on an as-needed basis, whichever is applicable.
The Ovation pipette can be easily in-lab calibrated ±10% for optimum performance at your operating conditions. In addition, the calibration factor can be pre-determined and set at the appropriate value for a liquid to be dispensed. Use Ovation’s calibration software to quickly and easily determine an appropriate calibration factor. Returning the calibration factor to 1.000 will re-set the Ovation pipette to its original factory calibration.
For optimum performance over the entire pipetting range, verify and calibrate the pipette using the maximum volume setting. For a specific liquid at one volume, verify and calibrate at that volume.
The Ovation macro models can be used with a wide variety of serological pipets. In order to achieve optimum accuracy for a particular brand pipet, it may be necessary to adjust the Ovation’s calibration.

1. Perform a verification at the desired volume.

2. Simultaneously press and hold the

buttons (about 3 seconds) until TONE

appears on the display. Press and release until CAL appears.

Note: To interrupt the calibration sequence at any time, depress and release the pipetting trigger. No changes will be saved.

3. Press or and Targ (target) and the pipette’s maximum volume is displayed.
4. Press or to change the volume, but only if calibrating at other than the maximum.
5. Press and MEAS (measured) and the maximum volume is displayed.
6. Press or to enter the actual volume dispensed during verification. (See Step 1)
7. Press and fact (factor) and a new calibration factor is displayed. Record this factor.
8. Press again and SET (set) appears on the display. After one second, the pipette accepts and stores the new calibration factor. A “C” may now appear on the display.
9. Re-verify volume delivery with this new calibration factor.

• For additional information on calibration verification, go to our documentation library under Support at www.vistalab.com

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

4 – 1

Entering a known Calibration Factor

Ovation allows the user to enter a pre-determined calibration factor for a specific liquid or volume, or return to the factory setting of 1.000.
1. Repeat Step 2 under “Determining a New Calibration Factor. Press until CAL appears on the LCD.
Note: To interrupt the calibration sequence, press and release the plunger. No changes will be saved.
2. Press or , followed by (2 times) to advance to FACTR on the display. Record this factor.
3. Press or to enter the known calibration factor.
4. Press and SET (set) then appears on the display while the pipette homes the plungers. The pipette has accepted and stored the new calibration factor.
5. Verify volume delivery at this new factor, or begin to use the pipette.

Calibration when using serological or volumetric pipets
(macro models only)

Due to the additional length of the air column or variations in serological or volumetric pipets, it may be desired to optimize performance by changing the internal calibration factor of the Ovation pipette. The factor may vary for different brands of serological or volumetric pipet used.
Note: For 100-5000µL models, install the Sero-adapter before beginning the calibration procedure. (See instructions on Page 2­5)

Record the current calibration factor as follows:

1. Simultaneously press and hold

(about 3 seconds) until TONE appears on

the display. Press and release until FACTR appears.

2. Record the number displayed. This is the calibration factor when using Ovation pipette tips. Record this value.

To set the calibration factor for use with serological pipets:

1. In the P1, P2 or PDB liquid handling function, set the macro Ovation to the maximum volume setting (i.e. 5000µl or 10.00mL).

2. Aspirate water or the liquid to be used with the serological pipet. Using the graduations on the pipet and the meniscus of the liquid, determine the volume aspirated. Dispense the liquid. Repeat aspiration and volume determination at least 3 more times. Average the results.

3. Simultaneously press and hold

(about 3 seconds) until TONE appears on

the display. Press and release until CAL appears.

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

4 – 2

Calibration when using serological or volumetric pipets (continued)
(macro models only)

4. Press once, and TARG will appear. Volume on display should be set to the maximum volume of the pipette from Step 1. Use and to adjust volume, if needed.
5. Press and release and MEAS will appear. Use or to adjust displayed value to the average volume dispensed in step 2 (ex. 4950µl).
6. Press again. The new calibration factor is displayed. Record this value which is the calibration factor to be used with this liquid with this variety of serological pipets.
7. Press and the Ovation Macro Pipette will automatically store this factor. A “C” may now appear on the display if the Cal factor is other than the factory setting of 1.000.
8. Verify accuracy of volume delivery by aspirating liquid and confirming that volume in serological pipet matches the volume displayed on the LCD.
9. In the event that a slight adjustment to calibration is needed, or to return to original factor, when FACTR is displayed, use or to make the change.
10. Record final factor and enter factor into pipette whenever serological pipets are used.

Ovation BioNatural Pipette ­ Electronic Single Channel Operator’s Guide

4 – 3

MAINTENANCE & TROUBLESHOOTING 5

Cleaning Exterior

The Ovation pipette requires minimal maintenance. Always store it in its “standing” position or on the Ovation Pipette Stand (Catalog No. 9058-4003) when not in use.
Clean outer surfaces as needed with a soft cloth dampened with warm water. To decontaminate outer surfaces, wipe with a 70% aqueous solution of ethanol or isopropranol.
WARNING! Before cleaning or decontaminating the pipette, make sure the power supply is disconnected.

Disinfecting Nozzles
Replacing a Nozzle on Single Channel Models

The replaceable nozzle contains an internal aerosol/liquid barrier filter to prevent liquid from being aspirated into the pipette. Additionally, the nozzle filter offers protection to internal parts from routine exposure to hazardous liquids and vapors. If this filter becomes wet, the pipette will not aspirate fluid until a new nozzle is installed or the nozzle filter is replaced.
Wipe the exterior surface of nozzles with disinfectant or a 10% bleach solution. Care should be taken not to get the barrier filter inside the nozzle wet, as the channel will not aspirate and dispense properly until corrective action is taken.
If the pipette is not aspirating and dispensing properly, it could be caused by (a) poor fitting alternate source tip, (b) nozzle is loose and needs to be tightened by turning it clockwise, or (c) a nozzle or nozzle filter needs to be replaced as liquid has been aspirated into it.

Extension Tube

Ejector Sleeve

Nozzle

NOTE: Wear gloves when doing this procedure.

1. Remove ejector sleeve by pulling it away from the pipette body to expose the nozzle assembly.

2. Place tubing provided on nozzle, and unscrew it in a counterclockwise direction. If it is suspected that the air tube has been contaminated, gently wipe the end of the air tube with a slightly dampened, lint-free tissue, then dry it off.

Note: Do not block or dislodge the metal piece that extends from the air tube of all except the Ovation 1000µL.

3. Place tubing on a new nozzle, and screw the nozzle onto the extension tube in a clockwise direction. Firmly tighten and remove tubing.

4. Slide the ejector sleeve over the nozzle assembly.

5. Push the sleeve firmly into place while using a slight rocking or twisting motion.

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

5 – 1

Replacing a Nozzle on 100-5000µL Macro Models

If the pipette is not aspirating and dispensing properly, it could be caused by (a) poor fitting alternate source tip, (b) nozzle is loose and needs to be tightened by turning it clockwise, or (c) a nozzle or nozzle filter needs to be replaced as liquid has been aspirated into it.

Ejector Sleeve

Extension Tube

Nozzle
NOTE: Wear gloves when doing this procedure.
1. Place a macro pipette tip onto the nozzle and push on ejector sleeve until click is heard. Grip tip firmly near the nozzle, and turn it in a counterclockwise direction to unscrew and remove nozzle from pipette.
2. Slide off the ejector sleeve. Place a gloved finger lightly against the end of the air tube, and press and release plunger to expel any liquid that may be present in the end of the tube. Gently wipe the end of the air tube with a slightly dampened, lint-free tissue, then dry it off.
3. Replace the ejector sleeve. Note: The ejector sleeve is slightly narrower on the end that is closer to the pipette body.
4. Screw a new nozzle onto the extension tube. Tighten by hand, or use a macro pipette tip to ensure the nozzle is firmly attached to the extension tube.

Replacing a Nozzle on 0.2-10mL Macro Models

Nozzle
1. Place a macro pipette tip onto the nozzle and push on ejector sleeve until click is heard. Grip tip firmly near the nozzle, and turn it in a counterclockwise direction to unscrew and remove nozzle from pipette.
2. Screw new nozzle into the ejector sleeve. Tighten by hand, or use a macro pipette tip to ensure the nozzle is firmly attached to the pipette.

Ovation BioNatural Pipette ­ Electronic Single Channel Operator’s Guide

5 – 2

Replacing a Nozzle Filter NOTE: Wear gloves when doing this procedure. on Single Channel Models
1. Remove the ejector sleeve.

2. Remove nozzle by placing the rubber tubing provided onto the nozzle, and unscrew it in a counterclockwise direction.

3. Remove the rubber plug at the tip end of the nozzle by inserting a straightened paperclip into the small hole in the plug. Use the inserted end of the paperclip to pry the plug out of the end of the nozzle.
4. Insert the paperclip into the threaded end of the nozzle and push out the old filter.

5. Pick up a new nozzle filter with tweezers and place it into the tip end (non-threaded) of the nozzle. The end of the filter with the visible ridge should be inserted first.

enlarged

6. Use the filter insertion tool to push the filter into position. Firmly press down until the filter is pushed to the bottom of the nozzle.

7. Tap the nozzle on the counter to remove any loose cellulose material.
8. Re-insert the rubber nozzle plug into the front end of the nozzle. The end of the plug without side ridges is inserted first. Press in until plug is even with surface edge of the nozzle. If filter is not completely seated, the plug will extend beyond the end of the nozzle. Remove plug and firmly seat filter with insertion tool.
9. Place rubber tubing onto the nozzle, and screw the nozzle onto the pipette in a clockwise direction. Firmly tighten and remove tubing from nozzle. Re- install ejector sleeve.

Ovation BioNatural Pipette ­ Electronic Single Channel Operator’s Guide

5 – 3

Replacing a Nozzle Filter on 100-5000µL Macro Models

NOTE: Wear gloves when doing this procedure.
1. Place a macro pipette tip onto the nozzle and push on ejector sleeve until click is heard. Grip tip firmly near the nozzle, and turn it in a counterclockwise direction to unscrew and remove nozzle from pipette.

2. Slide off the ejector sleeve and set aside.

Nozzle Ejector Sleeve

3. Remove the rubber plug at the tip end of the nozzle by inserting a straightened paperclip into the hole in the plug. Use the inserted end of the paperclip to pry the plug out of the end of the nozzle.
4. Insert the paperclip into the threaded end of the nozzle and push out the old filter.

5. Pick up a new nozzle filter and place it into the tip end (non-threaded) of the nozzle. The rounded end of the filter should be inserted first. Use finger to push the filter into position. Firmly press until the filter is pushed as far as it will go.

Note: The ejector sleeve is slightly narrower on the end that is closest to the pipette body.

6. Re-insert the rubber nozzle plug into the front end of the nozzle. Press in until plug is nearly even with surface edge of the nozzle. If filter is not completely seated, the plug will extend beyond the end of the nozzle. Press nozzle plug on countertop if necessary.
7. Place a gloved finger lightly against the end of the air tube, and press and release plunger to expel any liquid that may be present in the end of the tube. Gently wipe the end of the air tube with a slightly dampened, lint-free tissue, then dry it off. Replace the ejector sleeve.

8. Screw the nozzle onto the extension tube. Tighten by hand, or use a macro pipette tip to ensure the nozzle is firmly attached to the extension tube.

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

5 – 4

Replacing a Nozzle Filter on 0.2­10mL Macro Models

1. Place a macro pipette tip onto the nozzle and push on ejector sleeve until click is heard. Grip tip firmly near the nozzle, and turn it in a counterclockwise direction to unscrew and remove nozzle from pipette.

2. If nozzle filter needs replacement, with a gloved finger or pipette tip, lift out filter from center of ejector sleeve. Place a new nozzle filter into the ejector sleeve, and use finger to push it into position.

3. Screw nozzle (or new nozzle if replacing) into the ejector sleeve. Tighten by hand, or use a macro pipette tip to ensure the nozzle is firmly attached to the pipette.

Replacing a Nozzle Insert on 0.2­10mL Macro Models

The nozzle insert may need to be replaced if serological pipets leak or do not stay in.
1. Place a macro pipette tip onto the nozzle and push on ejector sleeve until click is heard. Grip tip firmly near the nozzle, and turn it in a counterclockwise direction to unscrew and remove nozzle from pipette.

2. Using a pipette tip, push nozzle insert out of the large end of the nozzle.

3. Place end of insert with larger hole into nozzle and push in all the way until it makes contact with end of nozzle.
4. Screw nozzle (or new nozzle if replacing) into the ejector sleeve. Tighten by hand, or use a macro pipette tip to ensure the nozzle is firmly attached to the pipette.
Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

Nozzle
Nozzle small hole
5 – 5

General Battery Information
Note: To extend battery life, the pipette will automatically enter “sleep” mode after 10 minutes of inactivity. Press any key or the pipetting trigger to resume operation.

The Ovation pipette’s battery must be recharged for approximately 90 minutes when the pipette is first received, or if the battery is fully discharged. To ensure the pipette has sufficient power for extended use, connect it to the power supply whenever it isn’t being used or when the battery icon on the LCD is flashing (indicating a low charge). Insert the power plug into recharging port and connect power supply to AC outlet. It may be left connected to the power supply indefinitely.
When a battery is fully discharged or is replaced, settings for all liquid handling functions and the current calibration factor remain stored in the pipette’s internal memory.
When fully charged, the pipette can perform approximately 600 cycles at full stroke at the highest speed setting. The number of cycles may vary, depending on pipette model, pipetting volume, and speed setting.
To Recharge the Battery:
1. Insert the power supply pipette plug into the recharging port and connect the power supply to an AC outlet.

Replacing the Battery
Note: To extend battery life, the pipette will automatically enter “sleep” mode after 10 minutes of inactivity. Press any key or the pipetting trigger to resume operation.

The lithium ion battery is a user replaceable part. When the battery requires more frequent charging, is not recharging or holding a charge, it should be replaced. Ordering information is available at www.vistalab.com.
Use of any other battery can cause damage to the pipette and void its warranty.
To Replace the Battery: 1. Loosen the captive screw on the base of the pipette and gently lower the base
away from the body, being careful not to stretch, loosen or disconnect the cable from the circuit board.

Note: If no battery is installed for an extended time and/or the volume display is blank, when the unit is recharged or the new battery is installed, the volume display will show an “r” value. To return the LCD to its routine display, press once. The LCD will display all character sets and then return to the last liquid handling function.
Note: When the battery is removed, the LCD contrast returns to the factory setting. To adjust the contrast, refer to page 3­9.

! Do not separate the front and back sections of the pipette body at any time. Doing so will void the pipette warranty.
2. Note the orientation of the battery. Lift the battery and gently disconnect it.
3. Reconnect a new battery and place the battery in the base of the unit.
4. Carefully fit the base onto the body of the pipette and tighten the captive screw. Be certain that cables and wires do not get pinched. Do not overtighten the screw.
5. Recharge the new battery before using the pipette or plug into power supply.

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

5 – 6

Troubleshooting

If the Ovation pipette fails to function as expected, review the following.
Symptom: Pipette display is blank
Probable cause: Battery is drained, connector not tight, crimped or broken connector wire, faulty AC power supply or AC power source, or LCD contrast may need to be adjusted.
Recommended action: If battery was recently replaced, check connector and wires for crimps or broken wire. Plug into an AC power supply and AC source. If Ovation display functions normally, then problem is a broken battery wire or the replacement battery is inoperative. Send to VistaLab for repair. See LCD contrast instructions on page 3-9 and adjust if possible.
Symptom: Pipette makes “alert” sound
Probable cause: Pipetting trigger was pressed before set-up of a liquid handling function was completed or an illegal action was performed (e.g. trying to enter set-up before a liquid handling function was completed).
Recommended action: Continue setting up function or exit setup. Continue pipette operation until liquid handling function is completed.
Symptom: Aspirating and dispensing is slow
Probable cause: The pipette speed is set to a low setting
Recommended action: Go to setup of the Liquid Handling Function and increase speed setting
Symptom: Battery icon is flashing
Probable cause: The remaining battery charge is low
Recommended action: Plug AC power supply into Ovation and to an AC source. Begin to use and allow to recharge at a convenient time.
Symptom: The pipette does not aspirate or dispense properly
Probable cause: Aerosol/liquid barrier filter in nozzle(s) is plugged due to aspiration of fluid into nozzle, a nozzle is loose or the battery is low.
Recommended action: Replace the nozzle for any channel(s) not aspirating. Check the battery status, and if necessary, recharge or operate with the pipette connected to the AC power supply.

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

5 – 7

Troubleshooting
(continued)
1
error
2
error
3
error
4
error
20
error

Symptom: Tips are not releasing from the nozzle Probable cause: Non-Ovation tips are being used Recommended action: Use Ovation tips.
Symptom: Pipette is leaking during pipetting Probable cause: Non-Ovation tips are being used, tip is not on securely, or inner piston or nozzle seal is worn Recommended action: Use Ovation tips. Tighten and/or replace the pipette nozzle. If it continues to leak, inner piston seal may need to be replaced. Send to VistaLab for maintenance
Symptom: ERROR 1 or ERROR 2 on display Probable cause: Motor stalled during aspirate (Error 1) or dispense (Error 2) cycle Recommended action: Press any keypad button and the pipette will automatically home its pistons. If error message continues, contact VistaLab Technical Service for assistance.
Symptom: ERROR 3 on display Probable cause: Sensor did not detect “home” position Recommended action: Press any keypad button and the pipette will automatically home the plunger. If error message continues, disconnect the battery for 3 seconds and retry. (Note: The LCD contrast returns to the factory setting when the battery is removed. See page 3-9 to change). If error message continues, contact VistaLab Technical Service.
Symptom: ERROR 4 on display Probable cause: Based on values entered for target and measured volumes, calculated CAL factor would exceed limits of .900 ­1.100. Recommended action: Verify entered values and change as needed.
Symptom: ERROR 20 on display Probable cause: Data corruption or check-sum error Recommended action: Contact VistaLab Technical Service for assistance.

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

5 – 8

Warranty

APPENDIX A
VistaLab Technologies, Inc. warrants the Ovation BioNatural Pipette against defects in materials and workmanship for one year from the date of purchase. To register your pipette and activate the warranty, register on the VistaLab Technologies web site at www.vistalab.com. This warranty is void if failure or damage is the result of improper handling, unauthorized modification, or use of ancillary products not supported by VistaLab Technologies. This warranty is exclusive; no other warranty is expressed or implied. Should the pipette need to be returned for calibration verification or service, go to support area of www.vistalab.com and follow the instructions for sending the pipette to VistaLab Technologies. Repack the pipette in its original packaging. Customer is responsible for shipping and insurance charges. If original packaging is unavailable, contact VistaLab Technologies for alternative packaging instructions.
Note: Damage to the pipette as a result of improper packaging is the responsibility of the customer.

Safety Compliance

Ovation pipettes have been tested and approved for safety labels:

EN 61010-1:1992 Safety Requirements CSA C22.2, No. 1010.1-92

EN 61326 EMC Requirements

UL 3111-1

Contact Information

To place an order or to send in your pipette for factory maintenance and/or calibration verification, see the support area of www.vistalab.com. Pipettes should be sent to:
VistaLab Technologies, Inc. Attn: Pipette Repair Department 2 Geneva Road Brewster, NY 10509 USA

For additional assistance with warranty repairs or other technical assistance, contact us at: 1-914-244-6226 (Worldwide) or 888-652-6520 (North America only), or send an email to techservice@vistalab.com

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

A – 1

Hazards and Precautions
! ! ! !

Proper use of Ovation pipettes as specified in this manual will ensure safe operation. However, please be aware of the following situations that can damage the device or cause serious personal injury.
WARNING! Connect power supply to a compatible power source. Refer to the voltage configurations shown below. Use of an incompatible power source can cause shock and fire hazard.
WARNING! Unplug power supply before cleaning the exterior. The power supply should be positioned for easy access so in case of emergency it can be quickly disconnected from the pipette. Fluid contact with internal components can cause shock hazard.
WARNING! If the pipette is used in a manner not specified, the protections provided may be impaired.
WARNING! The pipette, battery and power supply should be processed in accordance with applicable local regulatory directives concerning the waste management of electronic equipment when they are no longer of use. Contact your dealer for more information.
CAUTION! Use only the power supply and batteries provided by VistaLab Technologies, Inc. Use of other power supplies can damage the pipette and invalidate the warranty.
CAUTION! Do not immerse pipette in liquid. Fluid contact with internal components can damage the electronics and display.
CAUTION! Pipette is not intended for autoclaving. Autoclaving will damage the electronics and display.
CAUTION! Use only cleaning solutions specified in this manual to clean the exterior of the pipette. Use of other solvents can damage the exterior surface and keypad.

Operating Temperature & Environment Conditions
Indoor use / Pollution Degree 2 Altitude up to 2000m Temperature Range: 15°­35°C Relative Humidity Range, non-condensing: 10%­85% Atmospheric Pressure: 70­106kPa
Power Supply and Battery Specifications
Use only the power supply and batteries provided by VistaLab Technologies, Inc. Use of other power supplies can damage the pipette and invalidate the warranty.

Mains voltage fluctuation ±10% Direct Plug in class II power supply Installation class 2 for direct plug in power supply Ingress protection normal (IPX0) Battery: lithium ion

Symbols are visible on equipment: Symbol Description
Alternating current
Direct current
Attention – consult documents

Power Supply: Catalog #: 9060-9005 ­ Universal Power Supply, 100-240VAC, 50-60Hz. (Equivalent to 9060-9001, -9002, -9003 & -9004)

Pipette Body Chemical Compatibility Water, diluted ethanol or isopropanol, diluted bleach. For more information about chemical compatibility with internal seals or pipette tips, see the documentation library in the support area of our web site ­ www.vistalab.com.

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

A – 2

Tips and Accessories for the Electronic Single Channel and Macro Models

4060-1002 Catalog number

APPENDIX B

Notes: 1. Not for use with 5­250µL
Electronic Single Channel 2. Not for use with 25-1250µL
Electronic Single Channel 3. Filtered tips contain a
unique hydrophobic filter which acts as a barrier to aqueous liquids and aerosols, protecting the pipette and sample from trace amounts of carryover. 4. All 5mL tips are graduated, marked at 0.1mL increments
For the most complete information about specifications, tips, accessories and operating instructions, see our web site ­ www.vistalab.com

ACCESSORIES

F I LT E R E D

STERILE

NON-STERILE

Ovation ­ electronic single channel 0.5-20µL and macro volume models:

2-125µL 5-250µL

TIP SIZE: MICRO

SMALL

VistaRak 192 tips/rack, 5 racks
VistaStak, 192 tips/layer, 5 layers (small size) or 3 layers (micro and large size)
Stacked Rack, 200 tips/layer, 5 layers (small size) or 3 layers (large size)
Stacked Rack, Trace Metal Certified, 200 tips/layer 5 layers (small size) or 3 layers (large size)
Box 100 tips/box (5mL) or 35 tips/box (10mL)
VistaClear Box 35 tips/box
VistaBulkTM, 1000 tips/bag, or 250 tips/bag (5mL) or 100 tips/bag (10mL)
ProtectainerTM Bulk Pack, 1000 tips (small) or 750 tips (large)
Econo-PakTM Bulk Pack, 1000 tips
VistaRak, Sterile, Pyrogen-free, RNase/DNase certified, 192 tips/rack, 5 racks
VistaClear Box ­ Sterile, 60 tips/box (5mL) or 35 tips/box (10mL)
VistaTipTM Individually Wrapped Sterile, 200 tips
VistaTip Individually Wrapped, Sterile, Pyrogen-free, 200 tips

4060-1002 4060-9024
4058-1000 4060-1032

4060-2004 4060-9025
9025 9022
4058-2000 40251 42251
4060-2132
20251 2027 1

VistaRak, Filtered, Sterile, Pyrogen-free, RNase/DNase certified, 192 tips/rack, 5 racks
2µL tip for Ovation 0.2-20µL, Ovation 1µL and Ovation 2µL only
VistaClear Box ­ Filtered, Sterile, Pyrogen-free, RNase/DNase certified, 60 tips/box (5mL) or 35 tips/box (10mL) VistaTip Individually Wrapped, Filtered, Sterile, Pyrogen-free, RNase/DNase certified, 50 tips Single Channel Nozzles, 10/box Single Channel Nozzle Filter Kit, 25/bag Single Channel Type A Nozzles, 5/box Single Channel Nozzle Extender Single Channel Ejector Sleeve Single Channel Cap Opener Macro Nozzles, 1/box Macro Nozzle Filter Kit, 10/box or 5/box Macro Ejector Sleeve Macro Nozzle Insert, 1/box Macro Sero-Adapter for serological pipets Power Supply (all Countries) Power Supply w/4 pipette connections Lithium Ion Battery Pipette Stand

4060-1332 4060-1333 4060-2332

9057-1010 9057-2010

n/a

9057-2009

n/a

9057-1012

9057-1011

9057-4004

25-850µL 25-1250µL
LARGE
4060-3004 4060-9026
9026 9023
4058-3000 40262 42262
4060-3132
20262 2028 2 4060-3332
9057-3010 9057-3009 9057-1016
n/a 9057-3011
9060-9005 9060-9006 9060-4001 9057-4003

100-5000µL
5mL
9048 4058-5000
4058-5102
4058-5133 4058-5332
9060-5010 9060-5009 9060-5011
n/a 9060-5012

0.2-10mL
10mL
9050 4058-6100 4058-6000
4058-6102
4058-6133 4058-6332
9060-6010 9060-6009 9060-6011 9060-6008
n/a

Ovation BioNatural Pipette ­ Electronic Single Channel and Macro Operator’s Guide

B – 1

References

• Adjustable Ergonomic Ovation Pipettes – Accuracy & Precision – Think Differently

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