VWR CO2 Incubator Basic User Manual
- August 13, 2024
- VWR
Table of Contents
VWR CO2 Incubator Basic
Specifications
- Product Name: VWR CO2 Incubator Basic
- Model Number: 50150229
- Revision: D
- Manufacturer: VWR International, LLC
- Location: Radnor Corporate Center, Building One, Suite 200, 100
- Matsonford Road, Radnor, Pennsylvania 19087, United States
Product Information
The VWR CO2 Incubator Basic is designed to provide a controlled environment
for cell culture applications, ensuring optimal growth conditions for cells.
Chapter 6: Operation
To operate the device:
- Prepare the device as described in sections 6-39 of the manual.
- Start operation following the instructions in section 6-40.
Chapter 8: Shut-down
To shut down the device:
- Follow the steps outlined in section 8-51 of the manual.
- Turn off the device using the power switch as instructed.
Chapter 11: Parts and Accessories
Refer to the lists provided in sections 11-71 for information on available
parts and accessories for the CO2 incubator.
Chapter 12: Technical Data
For technical specifications and data related to the CO2 incubator, consult
chapter 12 of the manual.
Chapter 13: Disposal
Proper disposal guidelines for the device are detailed in chapter 13 of the
manual.
FAQs
Q: What should I do if I encounter problems not covered in the operating
instructions?
A: If you encounter issues not adequately addressed in the manual, contact VWR
immediately for assistance and ensure your safety.
2024 VWR International, LLC. All rights reserved. This Instruction Manual is copyright protected. Rights resulting thereof, particularly reprint, photomechanical or digital post-processing or reproduction, even in part, are only allowed with the written consent of VWR International, LLC. This regulation does not apply to reproductions for in-plant use. The contents of this document are subject to change without notice. Concerning translations into foreign languages, the English version of these operating instructions is binding.
Trademarks All trademarks mentioned in the operating instructions are the
exclusive property of the respective manufacturers.
VWR International, LLC Radnor Corporate Center, Building One, Suite 200 100
Matsonford Road Radnor, Pennsylvania 19087 United States
VWR International, LLC provides this document to its customers with a product
purchase to use in the product operation. This document is copyright protected
This document is copyright protected and any reproduction of the whole or any
part of this document is strictly prohibited, except with the written
authorization of VWR International, LLC. The content of this document are
subject to change without notice. All technical information in this document
is for reference purposes only. System configurations and specifications in
this document supersede all previous information received by the purchaser.
Important safety information
Instruction of the operating personnel
These operating instructions describe the CO2 incubator Model VWR Air Jacketed
CO2 Incubator Basic
The CO2 incubator has been manufactured in keeping with the latest
technological developments and has been tested before delivery for its correct
function. It may, however, present potential hazards if it is not used
according to the intended purpose or outside of operating parameters
Therefore, the following procedures must always be observed to prevent
accidents: The CO2 incubator must be operated only by trained and authorized
personnel. For any operation of this device, the operator must prepare clear
and concise written
instructions in the language of the operating and cleaning personnel based on
these operating instructions, applicable safety data sheets, plant hygiene
guidelines, and technical regulations, in particular:
Which decontamination measures are to be applied for the CO2 incubator and
accessories
Which protective measures are to be applied while specific agents are used
Which measures are to be taken in the case of an accident Repairs to the
device must be carried out only by trained and authorized expert personnel.
Applicability of the instructions
The contents of the operating instructions are subject to change without
further notice. Concerning translations into foreign languages, the English
version of these operating
instructions is binding. Keep these operating instructions close to the device
so that safety instructions and
important information are always accessible. Should you encounter problems
that are not detailed adequately in these operating
instructions, please contact VWR immediately for your own safety.
VWR
CO2 Incubator Basic | 3
Chapter 1 | Important safety information
Warranty
VWR warrants the operational safety and functions of the CO2 incubator only
under the condition that: The device is operated and serviced exclusively in
accordance with its intended use and as
described in these operating instructions The device is not modified Only
original spare parts and accessories that have been approved by VWR are used
Inspections and maintenance are performed at the specified intervals The
warranty is valid from the date of delivery of the device to the operator.
Explanation of Signs
Symbols and signal words used in the operating instructions
WARNING Indicates a hazardous situation which, if not avoided, could result in
death or serious injury!
CAUTION Indicates a hazardous situation which, if not avoided, could result in
minor or moderate injury!
NOTICE Indicates a situation which, if not avoided, could result in property
damage! NOTE Is used for applicational hints and useful information.
Important safety information | Chapter 1
Pictographs used in the operating instructions
Pictographs point to concrete facts of a safety message or a property damage
message.
Electric shock!
Suffocation hazard!
Hot surfaces!
Wear safety gloves!
Wear safety goggles!
Harmful liquids!
Electrical hazard!
Signs on the device
Safety signs, test marks and marks of conformity placed on the device. Observe
operating instructions! CSA test mark: Test certificate for USA/Canada,
indicates that the product has met the requirements of nationally recognized
product safety standards.
CO2 Incubator Basic | 5
Chapter 1 | Important safety information
Overview safety information
Overview of he most important safety information used in this operating
instructions.
Carbon dioxide (CO2): As CO2 is rated as a harmful gas, certain safety
instructions must be observed when the CO2 incubator is started up and when
the device is operated.
NOTE!
Personnel instruction:
Personnel operating devices with CO2 supply must be instructed about the
particularities in the handling of CO2 before starting their work: Correct
operation of pressurized gas containers and gas supply systems Obligation to
report damages and shortcomings in CO2 supply lines Measures to be taken in
case of accidents or failures These instructions must be repeated in
appropriate intervals and must comprise the particular
operating instructions of the gas supplier
6 | CO2 Incubator Basic
WARNING
Suffocation hazard:
CO2 released in large amounts into the room atmosphere may cause suffocation.
If CO2 is released, initiate safety measures immediately! Leave the room
immediately and do not allow others to enter the room! Inform security service
or fire department!
NOTICE!
Overpressure:
The operating pressure of the gas applied to the device must not exceed 1 bar.
If the gas is supplied at a higher pressure, the valves integral to the device
may not close correctly and the gas supply control may be impaired! Set the
gas supply to a range between 0.8 bar min. and 1.0 bar max. and make sure that
this
pressure setting cannot be changed!
Pressure compensation opening:
To ensure permanent pressure compensation, the pressure compensation opening
must not be connected to an exhaust air system. The pipe of the pressure
compensation opening must not be extended or redirected!
Access port: If the access port is not used, it must be capped during
operation!
VWR
Important safety information | Chapter 1
Gas quality: CO2 must be of the following qualities: Purity 99.5% min or
Medical grade CAUTION Electric shock:
Contact with current-carrying components may cause a lethal electric shock.
Before connecting the device to the power supply, check plug and connection
line for damage. Do not use damaged components when connecting the device to
the power supply!
CAUTION Electrical hazard:
The power supply in the lab should have a residual current circuit breaker
installed.
Health hazard: The surfaces of the work space may be contaminated. Contact
with contaminated cleaning liquids may cause infections. Disinfectants may
contain harmful substances. When cleaning and disinfecting, always observe the
safety instructions and hygiene regulations! Wear safety gloves Wear safety
glasses Wear mouth and respiratory system protection gear to protect the
mucous membranes Observe the safety instructions of the manufacturer of the
disinfectant and of the hygiene experts!
Contamination hazard: The device can be used for preparing and processing
infectious substances so that the device or device components may become
contaminated. In case of a shut-down, the device must be decontaminated!
Before device components are discarded, they must be decontaminated! The
device components must be cleaned thoroughly; after the cleaning, they must be
disinfected or sterilized, as required by the application. Discarded devices
or device components must be provided with an appropriate certificate
showing the decontamination measures performed!
Chapter 1 | Important safety information
Hygiene regulations:
Prior to any operation, the user must clean and disinfect the work space in
accordance with the hygiene regulations set forth by the operator to protect
the cultures.
NOTICE!
Incompatible cleaning agents:
Some device components are made of plastic. Solvents may dissolve plastics.
Powerful acids or bases may cause embrittlement of the plastics. For cleaning
the plastic components and surfaces, do not use hydrocarbon-containing
solvents, detergents with an alcohol content of more than 10% or powerful
acids and bases!
Chloride-containing disinfectants:
Chloride-containing disinfectants may corrode stainless steel. Use only
disinfectants that do not affect stainless steel! Do not use
sodiumhypochlorite solutions such as Purex and Clorox. These can cause
corrosion and pitting of stainless steel. Do not use steel wood pads such as
Brillo. These pads deposit carbon particles in the work
space.
Alcoholic disinfectants:
Disinfectants with an alcohol content of more than 10% may form, in
combination with air, easily combustible and explosive gas mixtures. When
using such disinfectants, avoid open flames or exposure to excessive heat
during the entire disinfection process! Use such disinfectants only in
adequately ventilated rooms. After the disinfectant has been allowed to react,
wipe the cleaned device components
thoroughly dry! Observe safety regulations to avoid fire and/or explosion
hazard caused by alcohol-containing
disinfectants!
Water supply:
The water tray of the work space can hold up to 3.0 l of processed water. When
the running operation, always keep a sufficient quantity of processed water of
the following quality available: Demineralized and distilled or autoclaved for
sterilization
or Completely deionized and distilled or autoclaved for sterilization When the
water level falls below the lower limit, refill water!
8 | CO2 Incubator Basic
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Important safety information | Chapter 1
Intended purpose of the device
Intended use
The CO2 incubator is a laboratory device for preparing and cultivating cell
and tissue cultures. The device allows the simulation of the special
physiological ambient conditions for these cultures due to the exact control
of: Temperature CO2 content An increased relative humidity
The device has been designed for installation and operation in the following
fields of application: Laboratories for cytobiological and biotechnological
experiments of safety levels L1, L2,
and L3 Medical-microbiological laboratories in accordance with DIN 58 956
Laboratories in the central area of clinics and hospitals
The CO2 required for the incubator is supplied to the device from a separate
gas supply system, either from gas cylinders or from a central pressurized gas
container. The layout of the gas supply system must ensure that the operating
pressure of the gas supply lines can be set to a range between 0.8 bar (min.)
to 1 bar (max.) and that the pressure cannot be changed. Depending on the
capability of the gas supply system, several devices may be connected to one
gas cylinder. The CO2 incubator is suited for continuous operation.
Unintended use
Do not use cell or tissue cultures in the device that are not in accordance
with the regulations of safety levels L1, L2, and L3.
Do not use tissues, substances or liquids that: Are easily ignitable or
explosive Release vapors that form combustible or explosive mixtures when
exposed to air Release poisons
Chapter 1 | Important safety information
Standards and directives
The device is in accordance with the following standards and guidelines:CAN/
CSA C22.2 No. 61010-4-04 UL Std. No. 61010 (2nd Edition) Safety
specifications: Indoor Use Only Altitude Up to 2.000 meters Temperature +5
°C to 40 °C Humidity Maximum RH 80% for temperatures up to 31 °C, decreasing
linearly to 50% RH at
40 °C Mains Supply Fluctuations Mains supply voltage fluctuations not to
exceed ±10% of the
nominal voltage Installation Category II1 Pollution Degree 22 Class of
Equipment I
1Installation category (overvoltage category) defines the level of transient
overvoltage which the instrument is designed to withstand safely. It depends
on the nature of the electricity supply and is overvoltage protection means.
For example, in Cat II which is the category used for instruments in
installations supplied from a supply comparable to public mains such a
hospital and research laboratories and most industrial laboratories, the
expected transient over voltage is 2500 V for a 230 V supply and 1500 V for a
120 V supply.
2Pollution degree describes the amount of conductive pollution present in the
operating environment. Pollution degree 2 assumes that normally only non-
conductive pollution such a dust occurs with the exception of occasional
conductivity caused by condensation.
VWR
2
Delivery
Packaging
The CO2 incubator Helix is delivered in a stable packaging box. All packaging
materials can be separated and are reusable: Packaging carton: Recycled paper
Foam elements: Styrofoam (CFC-free) Pallet: Untreated wood Packaging film:
Polyethylene Packaging ribbons: Polypropylene
Components standard equipment
Delivery
Shelf Shelf support post Shelf support rail Insert for pressure compensation
opening Plug for pipe channel Power supply cable Connector, potential-free
contact Spare caps, set CO2 connecting hose set Immersion water pump Open-end
wrench, 24 mm Allen wrench 2 mm for blower wheel Allen wrench 3 mm for blower
wheel cover Operating instructions Pump for water draining
CO2 incubator with single glass door and with continuous shelves (standard version) 3 4 6 1 1 1 1 1 1 1 1 1 1 1 1
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CO2 Incubator Basic | 11
Chapter 2 | Delivery
Acceptance inspection
After the device has been delivered, check the delivery immediately for:
Completeness Possible damages
If damages are detected or if components are missing, please contact the
carrier and vendor immediately.
12 | CO2 Incubator Basic
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3
Installation of the device
Ambient conditions
The device must only be operated in locations that meet the particular ambient
conditions listed below.
Requirements:
Draft-free and dry location.
The minimal distance to adjacent surfaces must be observed on all sides (see
Section “Space requirements”).
The operating room must be equipped with appropriate room ventilation.
Solid, level, fire-proof surface.
Vibration-proof substructure (floor stand, lab table) capable of bearing the
weight of the device and accessories (particularly if several devices are
stacked).
To ensure a constant incubation temperature of 37 °C, the ambient temperature
must be within a range of +18 °C to +33 °C.
Relative humidity up to 80% (max.).
Avoid direct exposure to sunlight.
Devices that produce excessive heat should not be used near the location of
the CO2 incubator.
Room ventilation
When CO2 is supplied, the work space of the incubator is slightly pressurized.
The pressure is released through the pressure compensation opening into the
operating room. As the pressure compensation and any opening of the glass
door/gas tight screen during the operation of the device will release very
small quantities of CO2 into the operating room, the room ventilation must be
capable of carrying the released gas safely off into the open. In addition,
heat dissipating from the device during permanent operation may cause a change
in the room climate.
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CO2 Incubator Basic | 13
Chapter 3 | Installation of the device
Therefore, the incubator must only be installed in rooms with sufficient
ventilation. Do not install the device in room recesses without ventilation.
The room ventilation should be a technical ventilation that is in accordance
with the
requirements of DGUV Information 213-850 (Guidelines for laboratories) or some
other suitable ventilation system with appropriate capacity.
Space requirements
14 | CO2 Incubator Basic
Figure 1 Device dimensions
When installing the device, make sure that the installation and supply
connections are freely accessible. The control box at the rear panel of the
device may serve as a spacer to adjacent objects. The side distances given are
minimal distances. To protect the CO2 incubator against contamination, use a
floor stand even if the device is installed near the floor. The height of the
floor stand should not be less than 200 mm. Several floor stands and carriers
are available as options from VWR (Part no. see Chapter 11 “Parts and
accessories”).
VWR
Installation of the device | Chapter 3
NOTE! Accessibility of the devices: To ensure the accessibility for care and
maintenance works, keep larger side and rear distances!
Transport
Figure 2 Lift point
For transport do not lift the device using the doors or components attached to
the device (e.g. control box on rear panel) as lift points. NOTE! Lift points:
Lift the device only using the lift points shown in Figure 2.
Electrical Installation
CAUTION Electrical hazard:
The power supply in the lab should have a residual current circuit breaker
installed.
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CO2 Incubator Basic | 15
Chapter 3 | Installation of the device
Stacking
16 | CO2 Incubator Basic
Figure 3 Stacking
Two Helix devices can be stacked on top of each other. The stacking elements
[1] and [2] secure the devices on top of each other. If the devices are placed
onto mobile racks, ensure that the rollers [3] are secured by an arresting
device during operation. For reasons of stability, the rollers should be
oriented to the front. NOTE! Transporting stacked devices: The stacking
elements are not connecting elements. Therefore, the transport of stacked
devices on sloped surfaces is not allowed!
Modifications
NOTE! Modifications: Retrofits and modifications must only be performed by the
Technical Service of VWR!
VWR
4
Description of the device
Safety devices
The device is equipped with the following safety devices: A door switch
interrupts the CO2 supply and the work space heating when the glass door is
opened An independent thermal protection protects the samples from harmful
overheating in the
event of a failure A pressure compensation opening ensures pressure
compensation in the device work space Audible and visual alarms indicate
failures during operation
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CO2 Incubator Basic | 17
Chapter 4 | Description of the device
Views of device
Figure 4 General views of device
Front view: [1] Stacking elements [2] Plug caps [3] Glass door [4] Measuring cell with blower wheel and sensors [5] Door switch [6] Pressure compensation opening with insert [7] Measuring opening
18 | CO2 Incubator Basic
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[8] Outer door [9] Door handle [10] Outer door seal, replaceable [11] Stand, height-adjustable [12] Nameplate [13] Power switch [14] Support rail [15] Shelf [16] Latch, glass door [17] Support hook for shelf [18] Access port with plug [19] Glass door seal, replaceable Rear view [20] Stacking elements [21] Access port, Ø 42 mm [22] Pressure compensation opening [23] Switchbox with supply interfaces [24] CO2 gas container
Description of the device | Chapter 4
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CO2 Incubator Basic | 19
Chapter 4 | Description of the device
20 | CO2 Incubator Basic
Work space atmosphere
In the work space of the incubator, the particular physiological ambient
conditions for the preparation and cultivation of cell and tissue cultures are
simulated. The work space atmosphere is determined by the following factors:
Temperature Relative humidity CO2 concentration Temperature:
To ensure undisturbed operation, the temperature in the operating room must be
at least 18 °C and the incubation temperature must be at least 3 °C higher
than the room temperature. The heating system controls the incubation
temperature from this temperature threshold up to 55 °C. The principle of air
jacket heating and the additional, separate heating of the outer door minimize
the risk of condensate formation on the side walls, at the ceiling of the work
space, and at the glass door.
Relative humidity:
The water tray of the work space can hold 4.5 liters of processed water. The
heating of the work space causes evaporation of the water, thereby ensuring a
constant humidity within the work space. Under normal operating conditions and
at the usual incubation temperature of 37 °C, a constant relative humidity of
approx. 95% is achieved in the work space. If heated containers are removed
and put back into the work space, the elevated humidity and the cooling may
cause condensate to form on the outer sides of the container.
For humidification, processed water of the following quality is required:
Demineralized and either distilled or autoclaved for sterilization
or Completely deionized and either distilled or autoclaved for sterilization
CO2 supply: To ensure correct growth conditions for the cell and tissue
cultures, the work space is supplied with CO2. The pH of the bicarbonate-
buffered culture media largely depends on the CO2 content of the work space
atmosphere. The CO2 content of the work space atmosphere can be controlled
within a range of 0 – 20%.
The CO2 must be of the following quality: Purity 99.5% min
or Medical grade
VWR
Sensor system
Description of the device | Chapter 4
Figure 5 Temperature sensor
The blower wheel and two sensor modules are integrated in the baseplate [1] of
the measuring cell:
Sensor [2] for the measurement of the work space temperature and of the
thermal protection
CO2 sensor [3] for the measurement of the CO2 content in the work space
atmosphere
The sensor for the measurement of the work space temperature as well as the
CO2 sensor form part of the control system of the device. Their measured
values are compared to the set nominal values. Based upon this data, the
control system controls heating and CO2 supply. The blower intermixes the
supplied gases and ensure uniform temperature distribution within the work
space.
The thermal protection has been preprogrammed at the factory and cannot be
changed. It protects the stored cultures from overheating. If the temperature
is exceeded by more than 1 °C, the thermal protection responds and the work
space temperature is automatically reduced to the set nominal value so that
the incubation process can be continued even in case of a failure. Any
response of the thermal protection will simultaneously trigger an audible and
visual alarm.
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CO2 Incubator Basic | 21
Chapter 4 | Description of the device
Door switch
Figure 6 Door switch
A door switch [1] is installed in the upper edge of the work space opening. If
the door switch is activated by opening the glass door, the gas supply and the
heating of the work space are interrupted and the display text will flash. If
the door remains open for more than 30 seconds, a short acoustic alarm sounds.
If the door remains open for more than 10 minutes, the acoustic alarm sounds
continuously. The outer door can only be closed after the glass door has been
latched properly.
22 | CO2 Incubator Basic
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Description of the device | Chapter 4
Switchbox with supply interface
Figure 7 Supply interfaces
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CO2 Incubator Basic | 23
Chapter 4 | Description of the device
All supply connections are installed in the switchbox at the rear of the device.
Gas connection:
The gas supply line between the device and the gas supply system is connected
using the supplied connecting hoses. CO2 is supplied to the device through a
separate connecting sleeve [1]. The process gas must be supplied to the device
at a fixed pressure that has been preset within a range of 0.8 – 1.0 bar, this
must remain unchanged. Before the gas is fed into the work space, it flows
through a sterile filter with a separation rate of 99.97% for a particle size
of 0.3 m (HEPA filter quality).
Label:
The label [2] contains information about gas supply, an alarm contact terminal
legend, and notes about the electrical fusing of the device.
RS 232 interface:
Via the RS 232 interface [3], the incubator can be connected to the serial
interface of a PC. This connection allows the computer-aided acquisition and
documentation of the major operating parameters (temperature, CO2
concentration, failure codes, etc.).
Alarm contact:
The device can be connected to an on-site, external alarm system (e.g.
telephone system, building monitoring system, visual or acoustic alarm
system). For this purpose, a potential-free alarm contact [4] is preinstalled
in the device.
NOTE!
Alarm contact:
The alarm contact receives only messages caused by work space atmosphere
conditions (temperature or gas)!
Power supply connection:
The power supply connection [5] of the device is established via a cable with
a connector for non heating appliances. The holder for the two device fuses is
integral to the power supply socket.
24 | CO2 Incubator Basic
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Work space components
Description of the device | Chapter 4
Figure 8 Water space layout / Water reservoir
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CO2 Incubator Basic | 25
Chapter 4 | Description of the device
The working surface of the incubator has been designed to minimize the surface area. This helps to reduce contamination and promotes easy of cleaning.
Internal chamber:
All components of the work space are made of stainless steel and have a
burnished, absolutely smooth and easy-to-clean surface. Any embossings have a
large radius.
The components of the shelf system can be removed easily so that only the
easily treatable, surface-reduced internal chamber [1] remains in the work
space for cleaning and manual disinfection works.
Water reservoir:
The water reservoir [2] is integral to the internal chamber floor and inclines
toward the rear. The embossing in the water tray is used to indicate minimum
level [3] and the maximum level [4].
Heating system:
An air jacket heating is used for heating the work space. The arrangement of
the heating elements ensures that condensate formation above the water
reservoir is prevented as fast as possible. The outer door of the device is
also heated. The heat radiated onto the interior glass door prevents
condensate formation. The work space of the device always remains visible,
despite high humidity.
Shelf system:
The support rails [1] of the shelf system are perforated every 42 mm so that
the support hooks [8] can be inserted variably for any culture container size
required. The shelves [2] have an integrated tilt protection and withdrawal
stop. For details of the shelf system, see Chapter 5 “Start-up”.
26 | CO2 Incubator Basic
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Rear panel openings:
Description of the device | Chapter 4
Figure 9 Rear panel openings
A seal-able access port [1] allows cables, hoses or additional sensors to be routed into the work space of the device. A pressure compensation opening with insert [2] at the rear panel of the device allows a compensation between the pressures in the work space and in the operating room.
NOTE!
Operating conditions:
When accessories are to be operated in the work space of the CO2 incubator, the ambient condition requirements must be observed (see table below). The energy introduced into the work space affects the beginning of the temperature control range. When additional heating sources are introduced into the work space, condensation (e.g. at the glass door) may occur.
Introduced energy
0 W 5 W 10 W 15 W 20 W *RT = Ambient temperature
Control range of the temperature General
RT + 3 °C RT + 6.5 °C RT + 9.5 °C RT + 13 °C RT + 16 °C
Example: RT* = 21 °C 24 °C 27.5 °C 30.5 °C 34 °C 37 °C
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CO2 Incubator Basic | 27
Chapter 4 | Description of the device
90 °C decontamination routine
The 90 °C decontamination routine is used to decontaminate the complete work
space including all installed components and sensors. During this routine, a
moist and wet atmosphere with highly decontaminating effect is created for 9
hours at a temperature of 90° C. The effectiveness of the decontamination
routine has been tested and certified by independent institutes. Information
about these tests is available at request from VWR. The entire program run of
the decontamination routine will take approx. 25 hours. After the run has been
completed, the device must be reactivated using the autostart routine.
NOTE!
Thermal protection:
If the thermal protection for the device responds, the 90 °C decontamination
routine can only be started after the cause of the failure has been repaired
or reset.
28 | CO2 Incubator Basic
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5
Start-up
Preparing the work space
The incubator is not delivered in a sterile state. Before the initial start-
up, the device must be decontaminated. Before the decontamination is
performed, the following work space components must be cleaned: Support rails
Support hooks Shelves Work space surfaces Work space seals Glass door NOTE!
Decontamination: For details about the cleaning and disinfection of the
device, see Chapter 9 “Cleaning and disinfection”.
Installing the shelf system
Tools are not required for the installation of the shelf system. The support
rails are secured using spring pressure. After the support hooks have been
inserted into the rail, the shelves are pushed onto the support hooks.
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CO2 Incubator Basic | 29
Chapter 5 | Start-up
Support rail installation/removal:
30 | CO2 Incubator Basic
Figure 10 Support rail installation/removal
The support rails are held at the sides by embossing [2] and [5] and secured
by the embossing [1] and [6]. The support rails marked with ( ) are inserted
at the rear panel of the device with the locksprings [3] facing upward.
VWR
Start-up | Chapter 5
1. Position support rail [4] onto the lower embossing [6] and tilt toward the
work space side wall so that the rail is positioned over the two embossings
[5] and [2].
2. Clamp lockspring [3] behind upper embossing [1]. 3. To remove the support
rails, pull lockspring tab down and out of the embossing to remove rail.
Installing the shelf supports:
Figure 11 Shelf support/sheet insert installation
1. Insert the shelf supports [3] into the perforations [1] of the support
rail with the bar facing down.
2. Make sure that the two vertical elements [2] of the shelf support are
flush with the support rail.
Installing the shelves:
1. Push the shelf [4] onto the shelf supports with the tilt protection [5]
facing the rear panel of the device. The tilt protection [5] is also used as a
guide for the shelf.
2. Slightly raise shelf so that the withdrawal stop [6] can be routed over
the shelf supports.
3. Make sure that the shelf supports are positioned in the two tilt
protectors in a way that it can move freely.
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CO2 Incubator Basic | 31
Chapter 5 | Start-up
Levelling the device:
1. Position a bubble level on the center shelf.
2. Rotate the adjustable device stands using the supplied 24 mm wrench until
the shelf is positioned horizontally in all directions. Perform the adjustment
of the device stands from left to right and from rear to front.
Gas connection
NOTICE!
Gas quality:
CO2 must be of the following qualities: Purity 99.5% min
or Medical grade
CAUTION
Overpressure:
The operating pressure of the gas applied to the device must not exceed 1 bar.
If the gas is supplied at a higher pressure, the valves integral to the device
may not close correctly and the gas supply control may be impaired! Set the
gas supply to a range between 0.8 bar min. and 1.0 bar max. and make sure that
this pressure setting cannot be changed!
NOTE!
Pressure compensation opening:
To ensure permanent pressure compensation, the pressure compensation opening
must not be connected to an exhaust air system. The pipe of the pressure
compensation opening must not be extended or redirected!
Access port:
If the access port is not used, it must be capped during operation!
32 | CO2 Incubator Basic
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CO2 connection:
Start-up | Chapter 5
Figure 12 Gas pressure hose installation
The gas supply from the gas supply system to the device is achieved using the
supplied flexible gas pressure hoses:
1. Connect gas pressure hose [1] to the sleeve of the gas supply system.
2. Remove protective cap [3] from sterile filter.
3. Slide hose clamp [2] onto gas pressure hose and connect hose to the sleeve
[4] of the sterile filter [5].
4. Secure gas pressure hose to the sleeve of the sterile filter using the
hose clamp.
Power supply connection
WARNING
Electric shock:
Contact with current-carrying components may cause a lethal electric shock.
Before connecting the device to the power supply, check plug and connection
line for damage. Do not use damaged components when connecting the device to
the power supply!
The device must be connected only to a correctly installed ground fault
circuit interrupter and grounded power supply source: Fusing: T 16 A Circuit
breaker: G 16
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CO2 Incubator Basic | 33
Chapter 5 | Start-up
Connection to the power supply system:
Figure 13 Power supply connection
1. Before connecting the device to the power supply, check to see if the
voltage of the power supply corresponds with the specifications on the
nameplate at the front of the device. If the ratings given for voltage (V) and
current (A) are not correct, the device must not be connected to the power
supply.
2. Connect the connector for non-heating appliances [2] to the socket [1] at
the control box of the device.
3. Connect the grounding plug [3] of the power supply cable to a correctly
grounded and fused socket.
4. Make sure the power supply cable is not subjected to tensile or
compressive force.
34 | CO2 Incubator Basic
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Start-up | Chapter 5
RS 232 interface connection
The RS 232 interface has been designed for a cable connection with 9-pin
connectors and a contact assignment of 1:1. Connection of the device:
Figure 14 RS 232 interface
1. Turn PC off.
2. Connect the connector [2] of the serial interface cable [3] (not comprised
in the scope of delivery) to the socket [1] at the supply interface at the
rear of the device.
3. Connect the remaining other connector [4] to an unassigned slot COM 1/COM
2 etc. at the PC.
4. Turn PC on.
Transfer protocol: The interface must be configured as follows: 9600 baud, 8
data bits, 1 stop bit, no parity.
Command sequences: Data communication is achieved with a defined structure of
command sequences (frames).
Frame structure: <STX | command | data | BCC | ETX>
Command: Bit 0 – 3 = data field length in byte Bit 4 – 7 = command
Check sum: BCC = 1 – complement (command XOR data XOR … XOR data N XOR FFH)
Command list – Reading control loop data 0110 0001 (61H) Data: 0001 0000 (10H)
for temperature during incubation operation 0001 0001 (11H) for CO2
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CO2 Incubator Basic | 35
Chapter 5 | Start-up
Device response for temperature and CO2 Data: Nominal value x 10, (2 bytes,
integer) Nominal value, (4 bytes, floating point number) Internal use, (5
bytes for CO2, otherwise 7 Bytes)
Command list – Requesting failure codes Command: 1001 0000 (90H)
Data: none
Response – Reading failure codes
The microprocessor returns a total of 10 bytes (5 integer values). Each
integer value represents a current failure code in the assigned control loop
(incubation temperature, CO2 content, general). The failure code General
failure belongs to a superior failure that is shown simultaneously in all
displays (e.g. failure code 99). The failure codes for incubation temperature
and decontamination temperature are shown in the temperature display, the
codes for CO2 are shown in the CO2 display. Value “—” shows that there is no
current failure.
Faulty response from control unit:
If a returned response is incomplete or faulty, the CPU responds with an NAK
(15H, only 1 byte, without frame). Otherwise, the command code (with
pertaining length information) is regarded as a response and the data that may
be required is transmitted.
Particularities during data communication:
For the data communication between PC and microcontroller, the following
particularities must be observed: The microprocessor stores an int or unsigned
int value with the sequence
Example: Temperature data request and response
36 | CO2 Incubator Basic
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Start-up | Chapter 5
Connecting the alarm contact
NOTICE!
Expert work:
VWR warrants the operational safety and the operativeness of the device only if installation and repairs are performed properly. The connection of the device to an external alarm system must only be carried out by adequately trained and authorized expert electrical/telecommunication personnel!
Function:
When failures occur in the temperature or gas control circuits, an alarm message is issued to the connected alarm/monitoring system. The potential-free contacts (1 changeover contact) have been laid out for the following circuits:
Circuit Circuits with system voltage SELV circuits (cf. VDE 0100, Part 410)
SELV-E circuits (cf. VDE 0100, Part 410)
Voltage max. 250 V ~ 25 V ~ 60 V = 50 V ~ 120 V =
External fusing max. 6 A max. 2 A max. 1 A max. 1 A max. 0.5 A
Alarm relay
Operating state No failure, power off No failure, power on Failure X: Contact closed / O: Contact open
Contact 4 – 1 X O X
Contact 4 – 3 O X O
NOTE!
Switching structure:
For all failures reported by the device (sensor circuit open, deviation from
the nominal value and door open for more than 10 minutes), the alarm relay
changes state!
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CO2 Incubator Basic | 37
Chapter 5 | Start-up
Connection example:
Figure 15 Example of a connection scheme
The connector [5] for the connecting cable is comprised in the scope of
delivery. The values for the operating voltage of the external circuits and of
the fusing of the alarm system are given in the table above.
1. Connect the individual conductors [1] to [4] of the connecting cable as
shown in the wiring diagram.
2. Connect the connector [5] of the alarm system connecting cable to the
interface [6] at the control box at the rear panel of the device.
38 | CO2 Incubator Basic
VWR
6
Operation
Preparing the device
The device must only be released for operation after all major measures for the start-up have been taken (see Chapter 5 “Start-up”).
Prior to starting operation, the following device components must be checked for their correct function:
Gas hoses must be seated tight on the connecting filter and must be secured using a hose clamp.
The access port must be capped.
The pressure compensation opening must be permeable, its insert must be
installed in the work space.
The glass door seal must not be damaged. The glass door measurement opening must be capped. The shelf system components must be installed safely.
NOTE!
Hygiene regulations:
Prior to any operation, the user must clean and disinfect the work space in accordance with the hygiene regulations set forth by the operator to protect the cultures.
Water supply:
The water tray of the work space can hold up to 3.0 l of processed water. When the running operation, always keep a sufficient quantity of processed water of the following quality available:
Demineralized and distilled or autoclaved for sterilization or Completely deionized and distilled or autoclaved for sterilization
When the water level falls below the lower limit, refill water!
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CO2 Incubator Basic | 39
Chapter 6 | Operation
Starting operation
Starting and loading the device: 1. Fill the water tray with up to max. 3.0 l
of processed water. Do not exceed the upper level mark. 2. Make sure that the
CO2 supply system valve is open. 3. Turn the device on using the power switch.
4. Set nominal values for temperature and CO2 content on the operating panel.
5. Ventilate work space by leaving both device doors open until acoustic alarm
sounds. 6. Start device using auto-start routine. 7. Close device doors. 8.
The temperature control adjusts the temperature to the set nominal value, the
humidity rises. 9. When the temperature and relative humidity are constant,
automatic adjustment of the CO2
measuring system is performed. 10. The “auto-start” indicator goes off. 11.
The CO2 control supplies the set amount of CO2. 12. The device is ready for
operation. 13. Load work space with cultures. NOTE! Duration of the auto-start
routine: When the device is cold and when the ambient temperature is low, the
auto-start routine may take up to 10 hours! Loading: To ensure sufficient air
circulation and even heating of the samples, the loading surface within the
work space should be used up to max. 70%. Large objects in the work space that
dissipate heat may impair heat distribution!
40 | CO2 Incubator Basic
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7
Handling and control
Power switch
Figure 16 Power switch
Depending on which side the door hinges are installed, the power switch is
integral to the front cover of one of the front device stands. [1] Power
Switch [2] Front cover To turn the device on: Press the power switch [1]; the
switch illumination comes on. To turn the device off: Press the power switch;
the switch illumination goes off.
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CO2 Incubator Basic41
Chapter 7 | Handling and control
Operating panel
The operating panel is divided into three functional areas: 2 displays that
show numeric values for temperature and CO2 content 8 keys for selecting
functions and for entering data 6 LEDs that show functions or operating states
42 | CO2 Incubator Basic
3
18
10
11
9
12
7
16
8
5
14
6
13
15
Figure 17 Operating panel
[3] Temperature display [5] Key for setting temperature nominal value [6] Key
for increasing value [7] Key for reading failure codes / stopping acoustic
alarm [8] Key for activating auto-start [9] LED for indicating door (open
door) [10] LED for indicating active auto-start [11] LED for indicating active
over-temperature protection [12] LED for indicating active optional 90 °C
decontamination routine [13] Key for starting optional decontamination routine
[14] Key for starting cal function [15] Key for reducing value [16] Key for
setting CO2 nominal value [18] CO2 display
VWR
Handling and control | Chapter 7
Control self-test
After the device has been turned on, the control goes through a test routine.
- Turn the device on
Press power switch. All indicators on the operating panel come on, all displays show the numeric value 8 to indicate that the test routine is being run
The temperature display shows a three-digit number for the corresponding assembly/parameter set: P 1: Operating and display board P 2: Measuring cell P 3: Main board P n: Parameter number The CO2 display shows the software version/device version.
2. Test routine completed The temperature display shows the current temperature value, the CO2 display shows the current CO2 value.
NOTE! Factory pre-settings: Upon delivery of the device, the following nominal values have been set: Temperature: 37 °C CO2 content: 0.0%
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CO2 Incubator Basic | 43
Chapter 7 | Handling and control
Setting the nominal temperature value
1. Indicate the nominal value:
Press the
key.
The temperature display shows the current nominal value.
2. Enter the nominal value:
The nominal value can be increased or reduced in increments; if you keep the key depressed, the UP/DOWN function switches to a rapid increase/reduction; after approx. 3 seconds, another increase/reduction occurs.
To increase the nominal value:
Press the
keys.
To reduce the nominal value:
Press the
keys.
3. Accept and store the nominal value:
Release both keys.
The temperature display shows the current actual value measured in the work space.
Setting the CO2 nominal value
1. Indicate the nominal value:
Press the
key.
The CO2 display shows the current nominal value. 2. Enter the nominal value:
The nominal value can be increased or reduced in increments; if you keep the key depressed, the UP/DOWN function switches to a rapid increase/reduction; after approx. 3 seconds, another increase/reduction occurs.
Press the
keys.
To reduce the nominal value:
Press the
keys.
3. Accept and store the nominal value:
Release both keys.
The CO2 display shows the current nominal value measured in the work space.
44 | CO2 Incubator Basic
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Handling and control | Chapter 7
Activating the auto-start routine
The auto-start function is an automated routine for the start and the
subsequent adjustment of the CO2 measuring system. After the start, the device
control adjusts the temperature to the set nominal value while humidity is
generated. When temperature and relative humidity have reached constant
values, the CO2 measuring system is automatically adjusted to these values,
and the work space is supplied with the preset quantity of CO2.
NOTE!
Application of the routine:
To ensure that the specified accuracy of the CO2 measuring system is maintained, the device should always be started using the auto-start routine after the nominal temperature setting has been changed by more than 1 °C or after extended interruptions of the operation of the device. The auto-start routine should be run at least every three months on the occasion of cleaning and maintenance works.
Running the routine usually takes 5 to 7 hours. At low room temperatures and when the device is cold, it may take up to 10 hours until the auto-start routine has been completed. If the glass door is opened or if the power supply of the device is interrupted while the routine is running, the routine is interrupted and rerun after the glass door has been closed and after the power supply has been reestablished.
At the start of the auto-start routine, the work space atmosphere must consist only of ambient air. The floorpan must be filled with a sufficient quantity of water!
1. Open both doors until the acoustic alarm sounds after 30 seconds:
All current actual values flash on the displays, the “door” LED illuminates, after 30 seconds the acoustic alarm sounds.
2. Enter nominal values:
See Sections “Setting the nominal temperature value” and “Setting the CO2 nominal value”.
3. Activate the auto-start routine:
Keep the key
depressed for 5 seconds.
The auto-start LED flashes.
4. Close all device doors:
The temperature display shows the actual value, the CO2 display shows “run”, the “door” LED goes off.
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CO2 Incubator Basic | 45
Chapter 7 | Handling and control
5. Cancel the auto-start routine:
Keep the
depressed for 5 seconds.
The displays returns to normal operation (incubation operation).
NOTE!
Canceling the routine:
The auto-start routine can be canceled any time!
Failure code:
The canceling of the routine is indicated by a corresponding failure code. For a list and for a description of the codes, please refer to Section “Failure code list”.
Reading failure codes
The device is equipped with a failure diagnostic system. This system
recognizes failures during the operation and allows the allocation of failure
causes by numeric codes. Failure recognition is displayed by an acoustic and a
visual alarm at the operating panel. The diagnostic system stores the last 10
failures in the sequence of their occurrence. The failure table can be
requested and read.
If the cause of a failure cannot be repaired, please have the fault code and
the serial number of the device available when contacting Technical Service.
NOTE!
Response delay:
To prevent short term changes of the operating conditions from resulting in
repeated failure messages during the operation of the incubator, the
diagnostic system has a response delay:
After changes to nominal values: max. 152 min
After the glass door has been opened: max. 45 min
Other failure causes: max. 1 min!
Delay time reset:
If the set nominal value is reached during the specified period, the delay
time is reset to 1 min!
46 | CO2 Incubator Basic
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Handling and control | Chapter 7
Failure cause:
When the temperature nominal value and/or the CO2 nominal value is reduced, a failure message (code 101/201) may appear due to the slow reaction time of the atmosphere within the work space. Therefore, the device doors should be opened for some time if the nominal values are reduced
1. The audible alarm sounds:
2. Silence the audible alarm: Press any key. The audible alarm is silenced.
3. Read codes:
Keep the
key depressed.
If no failure is detected, each display shows three hyphens.
If the system detects a failure, the display that is assigned to the corresponding control circuit shows a failure code. Example: If the temperature display shows failure code 101, a failure in the temperature control circuit was detected.
NOTE!
Failure codes:
For a list and a description of the failure codes, please refer to failure code list in this section!
4. Scroll through the failure code table:
To read stored failure codes from the failure code table.
Keep the
key depressed.
To scroll, keep the
or
key depressed.
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CO2 Incubator Basic | 47
Chapter 7 | Handling and control
The temperature display shows the last 10 failure codes that had been registered. The CO2 display shows the number of the individual failure code within the table.
5. Exit the failure code table:
Release the
key.
The temperature and CO2 displays show the current actual values.
6. Erase the failure code table:
Keep the
depressed for 5 seconds.
The temperature and CO2 displays flash to indicate that the failure table has been erased.
48 | CO2 Incubator Basic
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Handling and control | Chapter 7
Failure code list
Code Description No values displayed
42
NV RAM read failure
43
NV RAM read failure
44
NV RAM faulty
54
Manipulated variable
failure
55
I2C bus failure
66
Deviation of temperature
sensors from one another
77
CO2 cal range exceeded
88
Failure upon auto-start
99
Device doors open
100
Temperature below
nominal value
101
Temperature above
nominal value
104
Temperature sensor
faulty
200
CO2 below nominal value
201
CO2 above nominal value
204
CO2 measuring cell
faulty
500
Temperature 90°C rou-
tine below nominal value
501
Temperature 90°C rou-
tine above nominal value
502
Failure in 90°C routine
Cause
Communication between display and CPU- main board faulty
NV RAM faulty, default values loaded
NV RAM faulty, mirrored values loaded
CO2 measuring cell values not overwritten, device works with default values
Calculation error, device performed RESET
Data transfer faulty, measured value quality dropped below 50%
Temperature signal plausibility doubtful
Max. adjustment value exceeded
Total time elapsed or max. adjustment value exceeded
Doors open for more than 10 minutes, door switch
Actual value < nominal value 1 °C
Actual value > nominal value +1 °C
Sensor circuit open/shorted
Act. val. < nom. val. 1% No CO2 Prepressure low Supply line blocked
Act. val. > nom. val. +1% Prepressure high
Sensor circuit open/shorted
Actual value < 85°C
Actual value > 85°C
Power failure during heating or holding phase
Repair Contact Service
Contact Service
Contact Service
Contact Service
Contact Service
Eliminate source of interference, e.g. cellular phone
Contact Service
Contact Service
Repeat auto-start
Close device doors, test door switch for correct function Contact Service
Do not exceed ambient temperature limit Contact Service
Check gas supply: Connect new gas cylinder Raise prepressure to 1 bar Check
supply line to device Check gas supply: Reduce prepressure to 1 bar Contact
Service
Repeat decontamination routine, contact service as required Contact service
Silence alarm by pressing key 90°C (2 times 5 sec.), then restart routine
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CO2 Incubator Basic | 49
Chapter 7 | Handling and control
Resetting the thermal protection
1. The Thermal Protection Active LED illuminates:
2. Turn the device off: Press the power switch. All indicators come off.
3. Turn the device back on: Press the power switch.
NOTICE!
Thermal protection:
When the cause of the failure (e.g. excessive temperature in the operating
room) has been repaired, the device is set to normal incubation operation
after it has been turned on again. If the cause of the failure cannot be
repaired with simple measures (e.g. by ventilating the room or by reducing the
temperature in the operating room), the thermal protection will respond again
immediately; in this case, contact Technical Service!
Shutting the device down
WARNING Contamination hazard: If the work space surfaces are contaminated,
germs my be transferred to the environment of the device. In case of a shut-
down, the device must be decontaminated! 1. Remove culture containers and all
accessories from the work space. 2. Pump water out off the water reservoir. 3.
Clean and decontaminate the work space and wipe device dry.
Turn device off using the power switch
4. Unplug power connector and protect it against accidental reconnection. 5.
Close the CO2 supply system shut-off valves. 6. Disconnect gas pressure hoses
from sleeve at the rear of the device. 7. Until the device is shut down, the
work space must be continuously ventilated: Leave the glass
door and the outer door open and secure them in this state.
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CO2 Incubator Basic | 51
Chapter 8 | Shut-down
52 | CO2 Incubator Basic
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9
Cleaning and disinfection
Decontamination procedures
The operator must prepare hygiene regulations for the decontamination of the
device in accordance with the application of the device.
Wipe/spray disinfection: is used as the standardized manual disinfection
procedure for the device and for all accessories.
Wipe/Spray disinfection
The wipe/spray disinfection is carried out in three stages: Predisinfection Cleaning Final disinfection
Recommended cleaning and disinfection agents:
NOTICE!
Incompatible cleaning agents:
Some device components are made of plastic. Solvents may dissolve plastics. Powerful acids or bases may cause embrittlement of the plastics. For cleaning the plastic components and surfaces, do not use hydrocarbon-containing solvents, detergents with an alcohol content of more than 10% or powerful acids and bases!
Chloride-containing disinfectants:
Chloride-containing disinfectants may corrode stainless steel. Use only disinfectants that do not affect stainless steel!
Do not use sodiumhypochlorite solutions such as Purex and Clorox. These can cause corrosion and pitting of stainless steel.
Do not use steel wood pads such as Brillo. These pads deposit carbon particles in the work space.
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CO2 Incubator Basic | 53
Chapter 9 | Cleaning and disinfection
54 | CO2 Incubator Basic
Alcoholic disinfectants:
Disinfectants with an alcohol content of more than 10% may form, in
combination with air, easily combustible and explosive gas mixtures. When
using such disinfectants, avoid open flames or exposure to excessive heat
during the entire disinfection process! Use such disinfectants only in
adequately ventilated rooms. After the disinfectant has been allowed to react,
wipe the cleaned device components
thoroughly dry! Observe safety regulations to avoid fire and/or explosion
hazard caused by alcohol-containing
disinfectants!
Recommended disinfectant:
A surface disinfectant recommended by VWR can be ordered under following part
numbers: Spray bottle, 250 ml: Part No.: 50052425 Refill bottle, 500 ml: Part
No.: 50051939
NOTE!
Description disinfectant:
Details for efficiency and approvals are available on request!
Preparing the manual wipe/spray disinfection:
WARNING!
Electric shock:
Contact with current-carrying components may cause a lethal electric shock.
Prior to cleaning and disinfection work, disconnect the device from the power
supply! Turn the device off using the power switch. Unplug power connector and
protect it against accidental reconnection. Check to see if the device is de-
energized!
CAUTION!
Health hazard:
The surfaces of the work space may be contaminated. Contact with contaminated
cleaning liquids may cause infections. Disinfectants may contain harmful
substances. When cleaning and disinfecting, always observe the safety
instructions and hygiene regulations! Wear safety gloves. Wear safety glasses.
Wear mouth and respiratory system protection gear to protect the mucous
membranes. Observe the safety instructions of the manufacturer of the
disinfectant and of the hygiene
experts!
VWR
Cleaning and disinfection | Chapter 9
Pumping water out of the water tray: The standard equipment of the device
comprises a mechanical suction pump for removing the water from the water
tray. The water drain works through gravity.
1. Place the water pump [1] on the lowest shelf [5].
2. Feed the inlet hose [4] through a hole [7] of the lowest shelf to the
water reservoir [8].
3. Prepare a bucket [2].
4. Suck the water, therefore pump the water pump using the hand grip [6]
about four times until water flows in the outlet hose.
5. Let the water run into the bucket [2] through gravity.
6. Wipe remaining water off from the floor plate of the water reservoir using
a cloth.
Predisinfection:
1. Remove all samples from the work space and store them in a safe place.
2. Spray disinfectant onto the surfaces of the work space and of the
accessories or wipe the surfaces clean using disinfectant.
3. Allow disinfectant to react as specified by the manufacturer.
NOTE!
CO2 sensor: Do not spray disinfectant onto the CO2 sensor in the baseplate of
the measuring cell! Removing accessories and shelf system:
1. Remove all shelves, then remove the entire shelf system from the work
space. For removal and installation of the shelf system, please refer to
Chapter 5, “Start-up”.
2. If required, remove the blower wheel and its cover from the baseplate of
the measuring cell. The wheel and the cover can be autoclaved.
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CO2 Incubator Basic | 55
Chapter 9 | Cleaning and disinfection
Removing blower wheel and cover:
56 | CO2 Incubator Basic
Figure 18 Removing blower wheel and cover
1. Remove the two retaining screws [3] of the cover using the supplied Allen
wrench (3 mm) and remove the cover.
2. The blower wheel [1] is secured to the axle by a set screw [2]. Remove set
screw using the Allen wrench (2 mm) and pull blower wheel off.
NOTE! Functional check: After the installation, check to see if the blower
wheel is securely attached to the axle and if it can rotate freely, then
secure cover using the screws! Cleaning the work space and accessories: 1.
Thoroughly remove dirt residues and deposits using a solution of tepid water
and dishwashing
agent. 2. Wipe surfaces clean using a clean cloth and plenty of clean water.
3. Remove cleaning liquid from water tray and wipe all surfaces of the work
space thoroughly dry. 4. Wipe accessories thoroughly dry. Final disinfection:
- Install shelf system and accessories. 2. Again, spray disinfectant onto the
surfaces of the work space and of the accessories or wipe the
surfaces clean using disinfectant. 3. Allow disinfectant to react as specified by manufacturer.
VWR
Cleaning and disinfection | Chapter 9
90 °C decontamination routine
The entire program run of the decontamination routine takes approx. 25 hours.
CAUTION Hot surface: The surfaces of the exterior door handle, work space,
particularly the glass door armatures and the interior sheet of the outer
door, are heated during the decontamination routine. During the routine run or
immediately after completion of the run, always wear safety gloves when
touching these surfaces; observe the warning indicator at the operating panel!
- After the cleaning, reinstall the shelf system components into the work
space. 2. Fill the water tray with 350 ml of processed water. 3. Turn the
device on using the power switch. 4. Activate decontamination routine. 5.
After the decontamination routine has been completed, remove the remaining
water using a
sterile cloth. 6. Turn the device off or restart the device operation using auto-start routine. NOTE! Duration of the auto-start routine: When the device is cold and when the ambient temperature is low, the auto-start routine may take up to 10 hours.
90 °C decontamination routine procedure
Figure 19 90 °C decontamination routine procedure
The routine is divided into four phases. Each individual phase or several
phases can be canceled (i.e. skipped). If the operating step “Cancel
decontamination routine” is executed, the routine moves to the next program
phase. To cancel the routine completely, the operating step must be executed
repeatedly until the remaining run time display shows the value 0. When the
glass door is opened, this operating step cancels the routine completely.
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CO2 Incubator Basic | 57
Chapter 9 | Cleaning and disinfection
The remaining run time of the decontamination routine designates the period
between the start or the current routine time state and the cooling down to
the preset temperature nominal value (± 2° C).
Heating phase: Remaining run time approx. 25 hours. A The work space is heated
to a temperature of 90° C while an elevated relative humidity is created. The
current decontamination temperature is shown at the temperature display.
Decontamination phase: Remaining run time approx. 23 hours. After the
decontamination atmosphere has been created, the decontamination phase with a
run time of 9 hours is started. If the door is opened during this time, the
decontamination routine is restarted automatically as soon as the door has
been closed.
Cool-down phase: Remaining run time approx. 14 hours. The device cools down
until the originally set temperature nominal value is reached.
Postheating phase: Remaining run time approx. 3 hours. During the postheating
phase, condensate within the device is eliminated as far as possible;
remaining condensate accumulates at the bottom of the work space.
Activating the 90 °C decontamination routine
Before running the decontamination routine, fill the water tray with 300 ml of water.
1. Turn the device on:
Press power switch.
All indicators on the control panel illuminate. The software version is shown
at the temperature display and at the CO2 display.
2. Ventilate work space:
Open both doors until the acoustic alarm sounds after 30 seconds. The current actual values flash at the displays, the door LED illuminates, the acoustic alarm sounds after 30 seconds.
3. Start the routine:
Keep the
key depressed for 5 seconds.
The 90 °C LED flashes.
Close the device doors:
The actual value is shown at the temperature display. The remaining run time
is shown at the CO2 display. The door LED goes off.
58 | CO2 Incubator Basic
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Cleaning and disinfection | Chapter 9
4. Complete the routine:
Keep the
key depressed for 5 seconds.
The display returns to the normal operating state (incubation operation).
Cancelling the 90 °C decontamination routine
1. Cancel a routine phase:
Keep the
key depressed for 5 seconds.
The routine advances to the next phase.
2. Cancel the routine completely:
To completely cancel the routine, each phase must be skipped individually by
pressing the key until a remaining run time of 0 hours is displayed.
When the last phase (postheating phase) has been skipped, the routine is
completely cancelled.
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CO2 Incubator Basic | 59
Chapter 9 | Cleaning and disinfection
60 | CO2 Incubator Basic
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10
Maintenance
Inspections and checks
To ensure the operativeness and the operational safety of the device, the
functions and device components listed below must be checked at different
intervals.
Daily check: Gas supply of the CO2 supply system Annual inspection: Tightness
of the glass door seal Permeability of the pressure compensation opening with
insert Functional check of the operating panel and of the device control
Electrical safety check in accordance with the relevant national regulations
(e.g. VBG 4)
NOTE!
Functional check:
If safety devices were removed or disabled for inspections, the device must
not be operated before the safety devices have been reinstalled and checked
for their correct function!
Service intervals
During normal operation, the following service routine must be performed:
Weekly service: Refill the work space water tray with fresh processed water
3-month service: Run auto-start routine Perform temperature and CO2
calibration
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Chapter 10 | Maintenance
Annual service:
Replace sterile filter
NOTE!
Service contract:
VWR offer a device-specific service contract that comprises all test and
service works required!
Preparing the temperature calibration
To determine the exact measured value of the integrated temperature sensor, a
temperature comparison measurement has to be performed every three months.
If a major temperature deviation is found during this check, a temperature
calibration is required. During this process, the temperature control of the
device is set to the value measured during the temperature comparison
measurement. Use a calibrated measuring instrument with an accuracy of ± 0.1
°C for this test. To minimize temporary temperature fluctuations during the
measurement, the measuring instrument is placed into the work space in an
isothermal container (e.g. a bowl filled with glycerol). The center of the
work space is the reference location for the comparison measurement.
NOTE!
Isothermal container:
Do not use a container filled with water as an isothermal container as the
evaporation of water will result in a lower temperature reading!
62 | CO2 Incubator Basic
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Comparison measurement procedure:
Maintenance | Chapter 10
Figure 20 Temperature calibration
1. Turn device on using power switch.
2. Set temperature nominal value and allow device to be heated. This may take
up to several hours.
3. Place measuring instrument [3] onto the A center area of the work space.
Alternatively, a temperature sensor may be positioned in this location. Route
the connecting cable either through the measurement opening [2] in the glass
door or through the access port [1] at the rear panel of the device.
4. Close doors.
5. Wait until the temperature value displayed on the measuring instrument has
stabilized.
6. Calibrate temperature control as described in Section “Temperature
calibration procedure”.
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CO2 Incubator Basic | 63
Chapter 10 | Maintenance
64 | CO2 Incubator Basic
Measurement example: Temperature nominal value: 37 °C Reference temperature:
36.4 °C
NOTE! Excessive work space temperature: Excessive work space temperature after
the calibration can be reduced by leaving the doors open for approx. 30
seconds!
Temperature calibration procedure
1. Activate calibration:
Keep the
key depressed for 5 seconds.
All operating panel indicators flash.
2. Indicate the nominal value:
Press the
key.
The preset value of 37 °C is displayed.
3. Enter the measured value (destination value):
Press the
keys.
or
Press the
keys.
Destination value e.g. 36.4 °C
4. Accept the destination value:
Press the
key.
The temperature display momentarily shows “CAL”,
Then the corrected actual value (measured destination value 36.4 °C) is displayed.
VWR
Maintenance | Chapter 10
5. Cancel the calibration process: Press any key. The temperature display and
the CO2 display show the actual values.
Preparing the CO2 calibration
To determine the exact measured value of the device integral CO2 sensor, a CO2
comparison measurement may be performed every three months. If a major
deviation is found during this check, a CO2 calibration is required. During
this process, the CO2 control of the device is set to the value measured
during the comparison measurement. Use a calibrated measuring instrument with
an accuracy of ± 0.3% CO2 for this test. Suitable instrument: Portable IR
readout instrument. (Part no. see Chapter 11 “Parts and accessories”). The
measuring sample is withdrawn through the seal-able measurement opening of the
glass door or through the access port at the rear panel of the device. The
comparison measurement must be performed when the device is completely stable.
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CO2 Incubator Basic | 65
Chapter 10 | Maintenance
Comparison measurement procedure:
66 | CO2 Incubator Basic
Figure 21 CO2 calibration
1. Turn device on using power switch. 2. Set CO2 nominal value and allow
device to heat up completely and to create humidity. This
process may take several hours. 3. Route the measuring instrument either
through the measurement opening [2] in the glass
door or through the access port [1] at the rear panel of the device. Wait
until the CO2 value displayed by the instrument has stabilized. 4. Remove
measuring probe, plug measurement opening and close doors. 5. Calibrate CO2
control as described in Section “CO2 calibration procedure”. Measurement
example: CO2 nominal value: 5% Measured value: 5.6%
VWR
Maintenance | Chapter 10
NOTE! Excessive CO2 content: Excessive CO2 content after the calibration can be reduced by leaving the device doors open for approx. 30 seconds!
CO2 calibration procedure
1. Activate the calibration:
Keep the
key depressed for 5 seconds.
All operating panel indicators flash.
2. Indicate the nominal value:
Press the
key.
The set nominal value of 5% is displayed.
3. Enter the measured value (destination value):
Press the
keys.
or
Press the
keys.
Destination value e.g. 5.6%.
4. Accept the destination value:
Press the
key.
The CO2 display momentarily shows “CAL”,
then the corrected actual value (measured destination value 5.6%) is displayed.
5. Cancel the calibration process: Press any key. The temperature display and the CO2 display show the actual values.
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CO2 Incubator Basic | 67
Chapter 10 | Maintenance
Replacing the particle filters
The particle filters have plastic threads and are screwed by hand into the
threaded hole on the control box.
Procedure for gas supply particle filter:
Figure 22 Particle filter replacement
1. Make sure that the gas supply is shut off. 2. Loosen hose clamp [4]. 3.
Remove gas hose [5] from sterile filter sleeve [2]. 4. Remove retainer [1]. 5.
Unscrew the particle filter [2] from the threaded hole [3]. 6. When installing
the new particle filter, make sure that the plastic thread is not canted.
Screw
filter in carefully all the way to the stop. 7. Install retainer [1]. 8.
Connect gas hose to particle filter sleeve and secure it using hose clamp.
Check to see if the
gas hose is securely seated on the sleeve.
68 | CO2 Incubator Basic
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Replacing the device fuses
Maintenance | Chapter 10
Figure 23 Device fuse replacement
The two identical device fuses [4] are installed in the fuse compartment [1]
next to the power plug receptacle of the device: Time delay fuses, 6.3 A (5×20
mm)
1. The fuse holder is secured to the fuse compartment [1] using two locking
tabs [2].
2. To remove the fuse holder, squeeze the two locking tabs and pull holder
[3] out of fuse compartment.
3. Remove faulty fuse from holder and install new fuse.
4. Slide fuse holder into fuse compartment and press holder on until locking
tabs are fully engaged.
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CO2 Incubator Basic | 69
Chapter 10 | Maintenance
Replacing the door seal
Figure 24 Door seal replacement
The door seal (magnetic seal) of the outer door is located in the retaining
slot. No tools are required to replace the seal.
1. Pull magnetic seal [3] out of the guide slot [1].
2. Position new seal at a corner [2] and press seal retaining rail [4] into
slot.
3. Make sure that the retaining rail taper is positioned correctly in the
slot [1] and that the seal is flush with the door frame.
70 | CO2 Incubator Basic
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11
Parts and accessories
Lists of parts and accessories
Technical Support: Phone +1
888-793-2300 9am-5pm EST
When contacting the Technical Support for parts and accessories, please have
the following information available: Model Number Serial Number Date Purchased
NOTE!
Repairs:
Use only original parts that have been tested and approved by VWR. The use of
other parts presents potential hazards and will make the warranty void!
Additional parts:
For additional parts please contact VWR Service Organization.
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CO2 Incubator Basic | 71
Chapter 11 | Parts and accessories
72 | CO2 Incubator Basic
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12
Technical data
TECHNICAL SPECIFICATIONS
DESCRIPTION
UNITS:
MECHANICAL
External dimensions
mm
Width x Depth x Height
in
Interior dimensions
mm
Width x Depth x Height
in
l Chamber volume
cu.ft
Shelves
mm
Width x Depth
in
Standard quantity
Piece
Maximum quantity
Piece
Maximum surface load
Piece
Maximum device overall load
kg
ib
Weight, without accessories
kg
ib
THERMAL
Ambient temperature range
°C
Temperature control range
°C
Temperature deviation at 37°C, time
°C
Temperature deviation, spatial (DIN 12880, Part 2) at 37 °C
°C
Duration of the auto-start routine, to 37 °C ambient temperature h 20 °C
Temperature recovery time, at 37 °C, door open 30 seconds
min
(to 98% of initial value)
Heat transfer to environment: at 37 °C during 90 °C decontamination
kWh/h kWh/h
VALUE:
637 x 867 x 766 25.1 x 34.1 x 30.2 470 x 607 x 530 18.5 x 23.9 x 20.9 approx.
151 approx. 5.3 423 x 455 16.7 x 17.9 3 10 10 / insertion shelf 30 66.1 70
(stainless steel) 154.3 (stainless steel)
+18…33 RT + 3 … 55 ± 0.1 ± 0.6 5 … 10
< 10
0.06 0.26
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CO2 Incubator Basic | 73
Chapter 12 | Technical data
74 | CO2 Incubator Basic
DESCRIPTION HUMIDITY Water quality
TECHNICAL SPECIFICATIONS UNITS:
Liquid quantity: Incubation operation 90°C decontamination operation Constant humidity at 37 °C Humidity recovery time, at 93% rH, door open 30 s (to 95% of initial value) CO2 GAS SUPLLY SYSTEM Gas purity Prepressure Measuring and control range Control deviation, temporal Recovery time, at 5%, door open 30 seconds (to 98% of initial value) CO2 MEASURING CELL Accuracy (absolute) ELECTRICAL SYSTEM Rated voltage Rated frequency Interference suppression (DIN VDE 0875) Type of protection (DIN 40 050) Protection class Overvoltage category (IEC 1010, EN 61010) Pollution severity (IEC 1010, EN 61010) Rated current On-site fusing: Fuse Circuit breaker Rated input EMC class OTHERS Sound pressure level (DIN 45 635, Part 1) Relative humidity of environment Location elevation
l ml % rH min min % bar % vol. % vol. min
% CO2 V Hz
A
kW
dB(A) % rH m NN
VALUE:
demineralized / distilled or autoclaved or completely deionized / distilled or
autoclaved.
max. 3.0 / min 1.2 300 approx. 93 approx. 20 (stainless steel)
min. 99.5 or medical quality min. 0.8 – max. 1 0… 20 ± 0.1 < 8
± 0.3
1/N/PE 120 V, AC 50/60 Interference level N IP 20 l ll 2 5.3 (120 V, AC)
T 16 A G 16 0.64 (120 VAC) B
< 50 max. 80 max. 2000
VWR
Average gas consumption:
Technical data | Chapter 12
Figure 25 CO2 consumption
VWR
CO2 Incubator Basic | 75
Chapter 12 | Technical data
76 | CO2 Incubator Basic
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13
Disposal
CAUTION
Contamination hazard:
The device can be used for preparing and processing infectious substances so
that the device or device components may become contaminated. Before device
components are discarded, they must be decontaminated!
The device components must be cleaned thoroughly; after the cleaning, they
must be disinfected or sterilized, as required by the application.
Discarded devices or device components must be provided with an appropriate
certificate showing the decontamination measures performed!
All device components can be discarded properly after they have been decontaminated properly.
NOTE!
Recycling service:
VWR offer, for a small fee, an environmentally compatible recycling service for discarded devices!
List of used materials:
Component Thermal insulation components Printed circuit boards
Plastic components, general Exterior housing Device rear panel Outer door Door
inner panel Operating panel housing and protective foil Magnetic door seal
Heating
Material Polystyrene foam EPS/PPS-Compound Enclosed electrical components coated with different plastics, equipped on epoxy resin-bound boards. Note material labeling Galvanized steel sheet, painted Galvanized steel sheet Galvanized steel sheet, painted Galvanized steel sheet, painted Polyethylene Magnetic core sheathed with EMPP Silicone-sheathed resistance-type wires
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CO2 Incubator Basic | 77
Chapter 13 | Disposal
Component Interior containers, installed components and shelves Plug for pipe channel Pressure compensation opening insert Glass screen Glass door seal, measurement opening Sensor block Blower wheel Measuring cell baseplate seal Cables Packaging
Material Stainless steel 1.4301
Silicone POM with brass sinter filter Soda-silicate glass Tempered silicone
Stainless steel 1.4301 Stainless steel 1.4305 Tempered silicone Plastic-
sheathed copper flexible Corrugated board, polyethylene film, and styrofoam
78 | CO2 Incubator Basic
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14
Device log
Record following information here: Nameplate information Work carried out
Maintenance work Repairs
VWR
CO2 Incubator Basic | 79
Chapter 14 | Device log
Device type: Serial number: Location:
Work carried out
Part number: Service number: Operator’s note:
Notes
Date
Signature
80 | CO2 Incubator Basic
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15
Certificate of decontamination
VWR
CO2 Incubator Basic | 81
Chapter 15 | Certificate of decontamination
82 | CO2 Incubator Basic
VWR
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