BRAND QuikSip BT-Aspirator Bottle-Top Extraction Devices User Manual
- October 27, 2023
- BRAND
Table of Contents
BRAND QuikSip BT-Aspirator Bottle-Top Extraction Devices
Read carefully before use!
Safety Instructions
This aspirating instrument is not designed for universal use. This instrument may be involved with hazardous materials, operations, and equipment. This Manual does not purport to address all of the safety problems associated with its use. It is the responsibility of whoever uses this instrument to consult and establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Please follow the safety instructions of the cell-culture™ Aspiration Con-troller.
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Every user must be acquainted with this Operating Manual as well as with the cell-culture™ Operating Manual before operation.
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Follow general instructions for hazard prevention and safety instructions; e. g., wear protective clothing, eye protection, and gloves. When working with infectious or other hazardous samples, all appropriate regulations and precautions must be followed.
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Observe the reagent manufacturer’s information and instructions.
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Use the instrument only for aspirating liquids, with strict regard to the defined operating range.
If in doubt, contact the manufacturer or supplier. -
Always use the instrument in such a way that neither the user nor any other person is endangered. Avoid splashes. Use only suitable vessels.
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Never carry the mounted instrument by the pump assembly or the valve block. Breakage or loosening of the plastic cylinder may lead to personal injury from potentially hazardous liquids.
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Never use force on the instrument.
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Only use original spare parts. Do not attempt to make any technical alterations. Do not dismantle the instrument any further than is described in the operating manual!
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Before use check the instrument for visible damages. If there is a sign of a potential malfunction (e.g., piston difficult to move, sticking valves or leakage), immediately stop aspira-tion. Consult the ‘Troubleshooting’ section of this manual (see page 18), and contact the manufacturer if needed.
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Aspirated liquids must be disposed of in accordance with governmental regulations.
Purpose
This instrument is designed for aspirating aqueous solutions and inorganic and
organic substances such as biological buffers, liquids for cell culture, etc.
Limitations of use
- vapor pressure up to 500 mbar
- density up to 2,2 g/cm³
- use between +15 °C and +40 °C
- viscosity 75 mm²/s (dynamic viscosity [mPas] = kinematic viscosity [mm²/s] x density [g/cm³]).
Operating limitations
- Valve block (H), filling tube (J), and valves (G, J) are autoclavable at 121 °C.
- The dispensing cartridge (E) and pump assembly (B) are not autoclavable.
- The cell-culture™ Aspiration Con-troller (K) is not autoclavable. The included tube and the adapters are autoclavable.
Operating Exclusions
The user has to ensure in each case the compatibility of the instrument with
the intended application. Never use this instrument with liquids that attack
PP, PE, or EPDM. If used in combination with the cell-culture™ Aspiration
Controller never use with liquids, which additionally attack silicone and PVC.
Please see our catalog for a listing of chemical compatibility with components
of this instrument. Please ask for further information.
Liquids that should not be aspirated include, but are not limited to:
- non-polar solvents like hydrocarbons and halogenated hydrocarbons
- concentrated or oxidizing acids.
Components
- A Piston lock
- B Pump assembly, PC
- C Piston, PE
- D Dispensing cylinder, PP
- E Dispensing cartridge, PP/PE F* Seals, EPDM
- G Discharge valve, PP/EPDM H* Valve block, PP
- I Locking nut, PP
- J Filling tube, PP, with filling valve, PP/EPDM K* cell-culture™
- L adapter for cell-culture™, PP
Parts in contact with the media
Preparing instruments for use
Warning!
Wear protective clothing, goggles, and gloves. Follow all Safety Instructions
and observe the Operating Exclusions.
Note:
Follow all instructions for proper assembly.
To control the vacuum, connect the cell-culture™ Aspiration Controller with the QuikSip™ BT-Aspirator using the included silicone tube. The link is affected by the included adapter.
Generating vacuum
Attention!
Use a bottle stand when using smaller or other potentially unstable bottles to
help protect against tipping. Observe all Safety Instructions.
Aspiration
Attention!
Follow all Safety Instructions and observe the Operating Exclusions.
Once the pump assembly has been pushed down, aspiration is accomplished by depressing the button on the cell-culture™. The aspiration rate is proportional to the pressure on the button. Releasing the button ceases aspiration.
Replacing the dispensing cartridge
Attention!
The dispensing cartridge is a wearing part. Replace the cartridge when the
piston is difficult to move or is not airtight. To prevent chemical injury,
the dispensing cartridge has to be first cleaned and then emptied prior to
replacement.
Note:
Follow the diagrams on pages 8/9 for replacing the dispensing cartridge.
- Depress the pump assembly.
- Disengage the piston lock to release the dispensing cartridge.
- Remove the pump assembly.
- Remove and discard the dispensing cartridge.
- The reverse process with a new cartridge.
Cleaning
The instrument must be cleaned in the following situations to assure correct operation:
- immediately if the piston becomes difficult to move
- prior to long-term storage
- before replacing the valves
- before replacing the dispensing unit
Depending upon the frequency of use, the dispensing cartridge should be replaced regularly.
Warning!
Follow all Safety Instructions.
For cleaning the cell-culture Aspiration Controller please follow the instructions in the corresponding cell-culture manual.
Normal cleaning procedure
Warning!
Carry the mounted instrument only as shown in the figure.
- mild laboratory detergent, e.g., Mucasol®
- suitable solvent, e.g., distilled water
Cleaning the valves
Normal cleaning procedure.
Troubleshooting
| Problem | Possible cause | Corrective action |
|---|---|---|
| Will not aspirate | – Dispensing cartridge not locked |
– Dispensing cartridge leaks
– Filling valve leaks or is sticking
– Connection between filling tube and tube of the cell-culture™ leaks
– Tube of cell-culture™ blocked or sticking
| – Lock dispensing cartridge.
– Mount new dispensing cartridge.
– Clean the filling valve or replace the valve and seal.
– Stick the adapter firmly onto the filling tube and pull the tube firmly onto the adapter.
– Clean tube or replace.
Piston and dispensing cartridge difficult to move| – Crystal deposition
– Improper liquid aspirated
| – Replace the dispensing cartridge.
– Check, if the liquid is proper to aspirate.
Liquid behind the piston of the dispensing cartridge| – Dispensing
cartridge leaks| – Replace the dispensing cartridge.
Aspirated liquid can’t be delivered into the bottle| –
Discharge valve sticking or damaged| – Clean valve or replace.
Technical Data
The liquid volume, theoretically to be aspirated with one stroke, is 25 ml. The tube length, the viscos- ity of the aspirated liquid and other losses however could result in reduc- tion of the aspirated volume.
Adapter (PP), pack of 1
Outer-thread for bottle thread Cat. No.
- 32, 33 22 7043 22
- 32, 33 25, 24 7043 25
- 32, 33 28, S* 28 7043 28
- 32, 33 45 7043 45
- 45 32, 33 7043 96
- 45 S* 40 7043 43
- 45 38 7043 97
- S 40 S* 60 (PE) 7043 48
buttress thread
Return for Repair
Attention:
Transporting hazardous materials without a permit is a violation of federal
law.
- Clean and decontaminate the instrument carefully.
- It is essential always to include an exact description of the type of malfunction and the media used.
- Shipment is at the risk and the cost of the sender.
Outside the U.S. and Canada:
- Complete the “Declaration on Absence of Health Hazards” and send the instrument to the manufacturer or supplier. Ask your supplier or manufacturer for the form.
Return for Repair
– continued –
In the U.S. and Canada:
- Contact BrandTech Scientific, Inc. and obtain authorization for the return before sending your instrument for service.
- Return only cleaned and decontaminated instruments, with the Return Authorization Number prominently displayed on the outside of the package to the address provided with the Return Authorization Number.
Warranty
We shall not be liable for the consequences of improper handling, use, servicing, operation, or unauthorized repairs of the instrument or the consequences of normal wear and tear especially of wearing parts such as pistons, seals, valves, and the breakage of glass as well as the failure to follow the instructions of the operating manual. We are not liable for damage resulting from any actions not described in the operating manual or if non- original spare parts or components have been used.
Subject to technical modification without notice. Errors excepted.
Disposal
For the disposal of instruments and tips, please observe the relevant national
disposal regulations.