iNSAtech WTS-B101484 Industrial Legionella Field Test Kit Instruction Manual
- June 3, 2024
- iNSAtech
Table of Contents
iNSAtech WTS-B101484 Industrial Legionella Field Test Kit
Industrial Legionella Field Test™ Kit
This kit is designed to test for Legionella in risk areas identified by CDC* such as:
- Pipe fittings in domestic and industrial hot and cold water systems.
- Cooling towers.
- Showers.
- Water tanks.
Overview
This test is used to detect the presence of Legionella pneumophila serogroup 1 bacteria in water samples from a wide range of sources. The test operates via a Lateral Flow Immunochromatographic Assay (LFICA).
Each kit contains the following:
- 5 x individual foil wrapped tests.
- 5 x hollow fibre filters.
- 5 x syringes containing recovery buffer.
- 1 x filtrate collection bottle.
- 1 x pipework adaptor with 1/2 inch and 3/4 inch female threaded connectors.
Note that this can also connect to a shower head thread.
The product is intended for use as part of an overall water treatment,
management and risk reduction approach and, as all testing methods including
lab culture testing, should NOT be used as the sole method for assessing risks
associated with Legionella bacteria.
This test is intended for the analysis of water samples only. It is NOT
intended for the diagnostic testing, in a clinical or medical situation, of
Legionnaires’ Disease in humans.
Limit of detection
Laboratory analysis has demonstrated that tests are positive for clean water samples containing 100 CFU/Litre Legionella pneumophila serogroup 1. The limit of detection (LOD) of the test is equivalent to 100 CFU/L when a 250 ml sample is filtered. If smaller volumes are processed the detection limit will be altered accordingly.
Suspended solid content in water samples affects filtration and test performance, including analytical sensitivity. Actual results will vary. Water samples with high levels of suspended solids may block filtration entirely. L. pneumophila serogroup 1 bacteria recovery from water samples can range from <10 to 100%, depending on water sample composition. This is similar to filtration concentration techniques used in other microbiological analysis methods.
Test operating limits
The test has been evaluated for operation on samples between 10–45°C
(50–113°F). The test has been validated for samples that filter in less than
10 minutes. Samples requiring greater than 10 minutes to filter may give
erroneous results. Samples requiring long periods to filter may be indicative
of poor system maintenance.
A wide range of non-oxidizing biocides and biodispersants have been checked
for cross reaction and interference with the test.
The test should not be used on systems treated with biguanide or tetrakis
hydroxymethyl phosphonium sulfate (THPS) based biocides.
Specificity
The test has been shown to be non-reactive with the following bacteria (at
1×108 organisms per sample):
- Acinetobactercalcoaceticus
- Aeromonas hydrophilasubsp. Hydrophila
- Bacillus subtilis
- Burkholderia cepacia
- Citrobacter freundii
- Citrobacter koseri
- Enterobacter cloacae
- Escherichia coli
- Klebsiella oxytoca
- Pseudomonas
- aeruginosa
- Pseudomonas fluorescens
- Pseudomonas putida
- Pseudomonas stutzeri
- Ralstonia pickettii
- Raoultella terrigena
- Streptococcuspyogenes
- Yersinia ruckeri
| Organism | ≥cfu/mL |
|---|---|
| L.p Sg-2,3,8,11,13,14 | 1.00E+08 |
| L.p. Sg-4,5,6,7,9,10,15 | 1.00E+07 |
| L.p. Sg-12 | 8.00E+06 |
| S.aureus | 2.00E+08 |
The Hydrosense Legionella pneumophila Sg-1 test has been shown to produce weak positive results with other Legionella pneumophila serogroups and S.aureus at the cfu/mL stated in the above table.
Storage
The test is intended for storage at room temperature 18–22°C (64.4–71.6°F). Do
not freeze. When stored correctly, the test will continue to operate within
design specification, until the specified expiration date.
Do not use the test or the recovery buffer syringe after the date specified on
the packaging of the test. Do not use any test where the foil packaging is
perforated.
Disposal
The test, filter, syringe and caps cannot be reused or recycled. The packaging
materials and this instruction leaflet can be recycled.
Disclaimer
Albagaia makes no warranties or representations regarding performance of the
products, or that the products are merchantable or fit for a particular
purpose. Albagaia expressly disclaims all other warranties and
representations, express or implied, or which arise by operation of law or
otherwise.
HYDROSENSE® is a trademark of Albagaia Limited, used world-wide and registered
in various territories.
Test procedure
For optimum results the test should be performed at room temperature. The foil wrapping should NOT be opened until immediately prior to running the test. If the foil is opened and the test is NOT performed within 60 minutes discard the test.
Step 1. Fit the pipework adaptor
Attach a pipework adaptor to an appropriate connector from which to obtain a
representative sample. Hydrosense recommends that a separate pipework adaptor
(Hydrosense part 100171) be fitted and retained at each sample point to avoid
cross contamination. The sample line pressure should not exceed 6 bar (87
psi).
Step 2. Take a sample
Remove the filter from the packaging and tighten
the cap at the end. Attach the filter to the pipework adaptor; push the open
end of the filter into the plastic luer fitting and twist the locking ring to
secure. Hold the filtrate collection bottle under the clear outlet port of the
filter. Open the system valve to allow the filtration to start, filter at
least 250 ml of water into the bottle and close off the valve.
If the sample takes longer than 10 minutes to filter then stop the filtration
process and measure the amount of liquid in the collection bottle before
proceeding to step 3. The detection limit will be altered.
Avoid generating aerosols when collecting or handling samples.
Step 3. Recover the bacteria
Disconnect the filter from the pipework adaptor by twisting the lock ring and
pulling the filter from the fitting. Hold the filter vertically with the cap
at the top and the open end pointing towards the floor. Remove the cap and
screw it onto the open (opposite) end of the filter. Now take the small red
capped syringe of recovery buffer, remove the red cap and attach the syringe
to the now open end of the hollow fibre filter with a twist and turn movement.
Rotate the filter and the syringe so the syringe is at the bottom.
- Pull the syringe plunger back to the 0.5 ml mark, then push the syringe all the way to the 0 ml mark.
- Repeat step (a) a further 2 times (total of 3).
- Draw the syringe back to the 0.5 ml mark to collect the sample then slowly push the syringe plunger in to the 0.1ml mark. Avoid creating air bubbles in the collected 0.1ml sample. If necessary, push and pull the syringe plunger again to remove any air bubbles. Disconnect the syringe from the hollow fibre filter.
- The syringe now contains 0.1 ml of a concentrated sample which is ready for testing. Incorrect use of the syringe can cause flooding of
the test (too much sample added) or failure to run (insufficient sample added). Please ensure that correct amount (0.1ml) of sample has been collected.
Step 4. Add sample to test strip
Remove the test strip from its foil wrapping, and place it on a flat surface.
Before use, the test should have two pale blue lines across the result window.
Place the recovery buffer syringe over the small sample window at one end of
the test strip. Depress the plunger to dispense the 0.1 ml of recovery buffer,
containing any bacteria, onto the test strip.
RECORD THE TIME. Allow the test to develop at room temperature for 25 minutes.
Leave the test strip sitting on a flat surface during development.
Step 5. Interpreting the results
After 25 minutes, examine the test strip in good lighting. The free Hydrosense
smartphone app can be used to read the test accurately and record test
results. If the test is not read within 30 minutes of adding the water sample,
it should be discarded and a new test should be run.
The test should show one of the following results in the large result window
on the test strip:
-
Two RED lines across the result window. The red line closest to the sample window may be very faint (pale pink). Any distinct line, no matter how faint should be considered to be a POSITIVE result.
OR -
One RED line across the result window at the end furthest from the sample window. This is a NEGATIVE result.
Positive Results
A positive test result indicates that Legionella pneumophila serogroup 1 was
present in the sample above the detection limit. If a positive result is
observed, consult your risk management plan or seek advice from a water
management specialist immediately.
Negative Results
A negative result indicates that Legionella pneumophila serogroup 1 was not
detected and the concentration was below the detection limit of the test.
Invalid Tests
In the unlikely event that a test does not show any red lines, or if it only
shows a line at the end closest to the sample window, or if the line furthest
from the sample window is very faint, then the test result is invalid. Repeat
the test.
Performance Factors
The test does not differentiate between viable and non-viable organisms. The
test will detect dangerous viable but non-culturable bacteria, which cannot be
detected by traditional laboratory techniques. A positive result does not
necessarily mean that viable bacteria are present.
A negative result does not mean that the system is completely free from risks
associated with Legionella bacteria.
The test detects Legionella pneumophila serogroup 1.
You can visit www.hydrosense-legionella.com, contact your supplier or email
hydrosense@albagaia.com to troubleshoot the
test.
References
Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>