Bioptic Qsep100 Advance Capillary Gel Electrophoresis Analyzer Instruction Manual

Bioptic Qsep100 Advance Capillary Gel Electrophoresis Analyzer

Getting start with Qsep100

Install Qsep100

1. Unpack Mailbox and tighten the air tube with connector
   

2. Plug the power cord into Qairbox
   (* Please check the Qairbox label on the power cord)
   

3. Plug the power cord and USB cord into Qsep100
   (Please make sure the label on the power cord is labeled Qsep100)
   

4. Connect the other side of USB cord to the operation computer.
   

5. Plug the other side of the air tube into Qsep100 and turn on the Mailbox
   

6. Remove the protecting foam before turning on Qsep100

Your Qsep100 is READY TO USE

*DO NOT switch on the instrument immediately after powering off Wait at least 5 seconds

Operation Quick Start

1. Turn on the power.
   

2. Double click the icon Q-Analyzer for Qsep100.

3. Q-Analyzer for Qsep100 software user interface:
   

4. Click “Connect” to link the instrument.
   
   Packing list of Cartridge Kit
   Cartridges Alignment Marker Mineral Oil Dilution Buffer Separation Buffer Buffer Tray Droppers 0.2 ml tubes.
   

5. Buffer and Alignment Marker preparation:

6. Add 1X Separation Buffer into “S” well and add diH2O into the others.

• Buffer height should reach the groove of wells.
  

  2. Alignment Marker preparation:
     Add ≥ 20 μl Alignment Marker (20 bp & 1000 bp, C109100) into the 0.2 ml tube, and then add 10 μl “Mineral Oil” on top of it.

  3. Click “Change Buffer”.
     

  4. Place Alignment Marker at MA-1 position and the buffer tray on the tray holder.
     For other Alignment Marker sizes, please place them in corresponding positions.

• Make sure the buffer tray has been placed correctly on the holder.

• Centrifuge the Alignment Marker to the bottom of the tube and make sure no air bubble appears.
  

  8. Hold the tray holder and use the thumb to press the Alignment Marker tube tightly down into the well.
     

  9. Click “Change Sample” and place the samples (≥ 20 μl).

• Centrifuge the samples to the bottom of the tubes and make sure no air bubble appears.
  

  10. Unpack Cartridge:
      Please follow the steps of “Unpacking Guide” in the cartridge box.

  11. Open the cartridge door and insert the cartridge with guiding groove facing the front.
      

  12. Close the cartridge door.

• Press the door and make sure to hear the squeeze sound .
  

  13. Click “Latch”.
      
      Cartridge information will be presented on the main window after the cartridge is latched.
      
      New Cartridge Calibration:
      New cartridge needs to be calibrated before use.
      Please proceed with the following steps.

  14. Click “OK”.
      

  15. Click “HV Check”

• The storage and transportation condition may influence the gel matrix and cause unstable current.
  Check current and see if it is stable or not. After HV Check, if the current is still unstable, please repeat this step 2-3 times.
  

  3. Click “Calibrate”

• Make sure the Alignment Marker has been placed in correct position.
  
  During Calibration
  Software will recognize peaks from Alignment Marker signal. DO NOT use Size Marker or DNA sample to perform “Calibrate”.
  
  • For troubleshooting, please refer to cartridge unpacking guide for more details.

  14. Click on the blank column and designate ① the sample locations, ② test method, sample duration, runs, ③ result name and ④ Para by following steps 14-1, 14-2, 14-3 and 14-4.
      

  15. Click “Sample Position” and mark the position of sample on the plate and then press “OK”.
      

  16. Click “Method” to select the analysis method.
      

• If you want to analyze the samples with Alignment Marker, please choose the appropriate Alignment Marker and then place it on the corresponding position. (Check ✓ the box) 3. Enter the result name.
  

  4. Click the icon “Para” and set the parameters. (Baseline Factor, Peak Threshold, Calculate, etc.)
     

  5. Click “Run” to start the analysis.
     
     Brief introduction of the Signal Chart:
     
     Please refer to operation manual for more details.

Introduction of the Control Panel

Customers Support

Contact Information:
Company Name: BiOptic Inc.
Office Address: (23141) 5F., No.6, Ln. 130, Minquan Rd., Xindian Dis., New Taipei City, Taiwan
Factory Address: (23141) 4F., No.108-3,Minquan Rd., Xindian Dist., New Taipei City, Taiwan
Tel: +886-2-2218-8726, Fax: +886-2-2218-8727, E-mail: service@bioptic.com.tw

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