WILSONWOLF 80000E GatheRex Cell Harvest Pump User Manual
- June 17, 2024
- WILSONWOLF
Table of Contents
WILSONWOLF 80000E GatheRex Cell Harvest Pump
Product Information
Specifications
- Model: 80000E / 80000Z
- Electrical Requirements:
- Input: 100-240VAC, ~1.3A, 50-60Hz, Single Phase
- Fuse Type: 5ET 3.15-R, Cartridge, Time Delay, 3.15A, 250 V, 5mm x 20mm (0.2 x 0.79), 5ET Series
- Detachable Power Cord: C13, 18AWG, 10A minimum, Grounded (3 Prong), UL 62, CSA-C22.2 No. 49
- IEC / UL: 61010-1 edition 3.1 and 61326-1:2012
- No adjustments for altitude are needed.
- GatheRex Model:
- 80000E – White ID tags on the tubing pinch clamps. Compatible with small diameter tubing (0.160 OD; blood transfer bag style) for media removal and cell recovery.
- 80000Z – Black ID tags on the tubing pinch clamps. Compatible with large diameter tubing (0.375 OD) for media removal and small diameter tubing (0.160 OD blood transfer bag style) for cell recovery.
Product Usage Instructions
Set-Up
- Set the GatheRex on a stable, level surface and plug in the power cord.
- Ensure the pinch clamps for the selected fluid pathways are open to the media and cell collection bags.
- Place the closed system cell culture device to the left of the GatheRex.
- Gather your desired culture media removal and cell recovery collection vessels.
- Using a sterile tube welder, connect your collection vessels to the proper tubing of the cell culture device.
- Alternatively, use the aseptic technique and the luer or quick-connect fittings to dock your collection vessels to the proper tubing of the cell culture device.
- Attach the provided Tubing Assembly to the GatheRex compressed air outlet port. Note that the GatheRex is the source of compressed air.
Resuspending Cells
- Swirl the remaining volume in the G-Rex device to resuspend the cells existing on the gas-permeable membrane.
- Tilt the device to allow the fluid to collect/pool where the internal harvest line touches the base/side of the vessel.
FAQ
-
Q: Can I sterilize the product?
A: No, sterilizing the product may cause damage or distortion to the equipment. -
Q: Can I attach the GatheRex to an external compressed gas source?
A: No, do not attach the GatheRex to any external compressed gas source. -
Q: Can I open the equipment cabinet?
A: No, opening the equipment cabinet poses a danger of electrical shock. -
Q: What are the compatible tubing diameters for each model?
A: Model 80000E is compatible with a 0.160-inch tubing diameter, while Model 80000Z is compatible with a 0.375-inch tubing diameter for media removal and a 0.160-inch tubing diameter for cell recovery.
PRODUCT DESCRIPTION
The GatheRex™ Cell Harvest Pump is a liquid handling device designed to work
with closed-system cell culture devices. The GatheRex connects to closed
system devices and uses a low-pressure forced air mechanism to remove media,
concentrate cells and minimize storage and/or downstream processing. An
internal air pump pressurizes the system by pumping air through the sterile
vent filter on the cell culture device. When pressurized, excess media, or a
cell fraction, is forced out of the device through the tubing assembly and
then into a collection receptacle. Model 80000E is designated by white ID tags
on the tubing pinch clamps. This version is compatible with small-diameter
tubing (0.160” OD; blood transfer bag style) for both media removal and cell
recovery. Model 80000Z is designated by black ID tags on the tubing pinch
clamps. This version is compatible with large-diameter tubing (0.375” OD) for
media removal and small-diameter tubing (0.160” OD blood transfer bag style)
for cell recovery.
PRECAUTIONS
- DO NOT sterilize this product. Equipment may be damaged or distorted.
- DO NOT immerse or overly wet the product during cleaning.
- DO NOT attach the GatheRex to any external compressed gas source.
- DO NOT position the equipment too closely to the side wall or equipment on its left or right. Allow space to left and right of GatheRex.
CONTENTS
- GatheRex Device
- Tubing Assembly – for Low-Pressure Outlet Port
- Power Cord
ELECTRICAL REQUIREMENTS
- Input: 100-240VAC, ~1.3A, 50-60Hz, Single Phase
- Fuse Type: 5ET 3.15-R, Cartridge, Time Delay, 3.15A, 250 V, 5mm x 20mm (0.2” x 0.79”), 5ET Series
- Detachable Power Cord: C13, 18AWG, 10A minimum, Grounded (3 Prong), UL 62, CSA-C22.2 No. 49
- IEC / UL: 61010-1 edition 3.1 and 61326-1:2012
LOW-PRESSURE OUTPUT
- Gauge Pressure: 120 – 180 mmHg (2.32 – 3.48 psi) No adjustments for altitude are needed.
- Flow Rate: Minimum 5 mL/sec fluid transfer rate from G-Rex M-CS
ENVIRONMENTAL CONDITIONS
- Temperature: 15°C to 35°C (59-95°F)
- Humidity: ≤ 95% Relative Humidity
- Altitude: < 2,000 meters (6,561 feet)
SET-UP
- Set the GatheRex on a stable, level surface and plug-in power cord.
- Place the closed system cell culture device to the left of the GatheRex.
- Gather your desired culture media removal and cell recovery collection vessels.
- a. Using a sterile tube welder, connect your collection vessels to the proper tubing of the cell culture device.
- b. Alternatively, use the aseptic technique and the luer or quick-connect fittings to dock your collection vessels to the proper tubing of the cell culture device.
- Attach the provided Tubing Assembly to the GatheRex compressed air outlet port. Note that the GatheRex is the source of compressed air.
- Fasten the other end of the Tubing Assembly to the outlet of the sterile air vent filter of the cell culture device by way of a luer connection.
- Position the Media Removal tubing into the GatheRex red clamp and the Cell Recovery tubing into the blue clamp.
- a. Open the covers of the pinch valve/sensor.
- b. Seat the tubing along the channel as shown.
- c. Close the covers.
- d. Run the tubing through the holding pins to keep them in position.
- i. Note: Large-diameter C-Flex tubing will not fit into the holding pins.
- e. Place the collection vessels to the right of the GatheRex.
- Turn on the GatheRex by flipping the switch with the power cord plug-in.
EXAMPLE OF USE – Media Reduction and Cell Harvesting from a closed system G-Rex device.
- Ensure the pinch clamps for the selected fluid pathways are open to the media and cell collection bags.
- Press the RED Right Arrow (à) to begin Media Removal (Reduction) through the red striped tubing or the large diameter CFlex tubing. The headspace of a closed system G-Rex device will be pressurized moving waste media to the collection bag. This will continue until the cell culture media level reaches the end of the internal media reduction line (~1 cm from the bottom). Once media removal from the G-Rex device has been achieved, media flow will stop automatically via a sensor located within the red tubing clamp.
- Swirl the remaining volume in the G-Rex device to resuspend the cells existing on the gas-permeable membrane. Tilt the device to allow the fluid to collect/pool where the internal harvest line touches the base/side of the vessel.
- With G-Rex still tilted, press the BLUE Right Arrow (à) to begin Cell Recovery (Harvest). The G-Rex will be pressurized moving the cell population and all remaining fluid into a Cell Harvest Bag. Media flow will stop automatically via a sensor located within the blue tubing clamp.
- Optional Steps to harvest any potential remaining cells in the GRex cell culture device by way of a rinse procedure:
- a. Partially refill the G-Rex with Media from the Red Removal Circuit by pressing the RED Left Arrow (ß) button. This releases the clamp for the red-striped removal conduit, allowing fluid to be gravity-fed or forced back into the G-Rex by squeezing the waste media bag manually or with a pressure sleeve.
- b. When the desired volume of media has been moved back into the G-Rex, press the RED STOP (X) button to re-clamp the red-striped removal conduit.
- c. Swirl the fluid within the G-Rex to capture and resuspend any remaining cells.
- d. With the device tilted towards the internal harvest line, press the BLUE Right Arrow (à) button for cell harvest. Media flow will stop automatically via a sensor located within the blue tubing clamp.
- Clamp the reduction line and the harvest line to seal the waste media collection bag and cell collection bag.
- a. Alternatively, simply seal the collection bags with a sterile tube sealer.
- Press the release clamps on the GatheRex Pump and remove the tubing.
- Seal the collection bags with a sterile tube sealer.
- Discard the G-Rex vessel and the waste culture media.
- See examples of setup with G-Rex at end of this document.
EXAMPLE OF SETUP – GatheRex 80000E and G-Rex100M-CS:
EXAMPLE OF SETUP – GatheRex 80000Z and G-Rex500M-CS:
MAINTENANCE AND SERVICE
Maintenance – There are no maintenance items on the GatheRex other than periodically wiping down of the exterior of the device with 70/30 IPA. Use care not to over-saturate or immerse device. If the power cord becomes damaged contact Wilson Wolf for a replacement. All repairs and services must be performed by Wilson Wolf.
Inspection – Pressure output should be verified before each use using an external calibrated pressure gauge (see air pressure verification details below).
Air Pressure (verification) – Note that the internal pressure regulator cannot be adjusted (i.e., output pressure can only be verified). To measure pressure output, connect a calibrated pressure gauge to the compressed air outlet port and tubing. An appropriately sized fluid-filled tube must also be placed into the pinch clamp/sensor to allow the pump to run for more than 6 seconds once initiated using the à key.
Fuse Replacement – Unplug cord from wall outlet. Remove Cord from Power
Module on back of GatheRex. Pry open top of access door of Power Module with
flat screwdriver. Use flat screwdriver to pry side of Fuse Carrier for
removal. Inspect and replace fuse (see page 1 for fuse type). Re-insert the
Fuse Module into GatheRex with the appropriate voltage positioned at the top
(i.e., 115V or 230V). Close the Power Module door and verify the desired
voltage is shown through window in Power Module door. Re-install Power Cord
and plug into a wall outlet.
TROUBLESHOOTING
- If Flashing Red Power LED – If the system is unable to build pressure (i.e., system leak) the GatheRex will run for approximately 33 seconds and then stop the air pump. The red power LED will flash red. To reset, turn device off and then back on again. Fix system leak before restarting cycle.
- If the GatheRex Air Pump will not run longer than 6 seconds – Verify that tubing is fully seated in pinch clamp and that fluid fills tubing within 6 seconds. If air is present in tubing the pump will stop.
- If Green Power LED will not light – Confirm outlet has power. Verify that power cable is fully seated in outlet and back of device. Confirm GatheRex power switch is turned ON. Inspect and/or replace Fuse per Maintenance and Service instructions.
MANUFACTURED BY
Wilson Wolf Manufacturing Corporation
St. Paul, Minnesota 55112 USA
Made in USA
TECHNICAL SUPPORT / ORDERING
- Tel (+1) 651.628.9259
- Email [email protected]
- Web www.wilsonwolf.com
Patent: www.wilsonwolf.com/patents
G-Rex® is a registered trademark of Wilson Wolf Corporation
GatheRex™ is a trademark of Wilson Wolf Corporation
© 2020 Wilson Wolf Corporation. All Rights Reserved.