WILSON WOLF 80000E GatheRex Cell Harvest Pump User Manual

WILSON WOLF 80000E GatheRex Cell Harvest Pump

Product Information

Specifications

• Model: 80000E/80000Z
• ELECTRICAL REQUIREMENTS:
  • Input: 100-240VAC, ~1.3A, 50-60Hz, Single Phase
  • Fuse Type: 5ET 3.15-R, Cartridge, Time Delay, 3.15A, 250 V, 5mm x 20mm (0.2 x 0.79), 5ET Series
  • Detachable Power Cord: C13, 18AWG, 10A minimum, Grounded (3 Prong), UL 62, CSA-C22.2 No. 49
  • IEC / UL: 61010-1 edition 3.1 and 61326-1:2012
  • No adjustments for altitude are needed.
  • Flow Rate: Minimum 5 mL/sec fluid transfer rate from G-Rex M-CS

Product Description
The GatheRex is a positive-pressure air pump designed for moving cell culture media and cells out of a closed system G-Rex cell culture product. It is available in two models:

• Model 80000E: Compatible with small diameter tubing (0.160 OD; blood transfer bag style) for both media removal and cell recovery.
• Model 80000Z: Compatible with large diameter tubing (0.375 OD) for media removal and small diameter tubing (0.160 OD blood transfer bag style) for cell recovery.

Environmental Conditions
Temperature and humidity range for proper operation: 11-0469 rev B

Product Usage Instructions

Set Up

1. Set the GatheRex on a stable, level surface and plug in the power cord.
2. Ensure that the pinch clamps for the selected fluid pathways are open to the media and cell collection bags.
3. Place the closed system cell culture device to the left of the GatheRex.
4. Gather your desired culture media removal and cell recovery collection vessels:
   • Using a sterile tube welder, connect your collection vessels to the proper tubing of the cell culture device.
   • Alternatively, use the aseptic technique and the luer or quick connect fittings to dock your collection vessels to the proper tubing of the cell culture device.
5. Attach the provided Tubing Assembly to the GatheRex compressed air outlet port. Note that the GatheRex is the source of compressed air.
6. Swirl the remaining volume in the G-Rex device to resuspend the cells existing on the gas-permeable membrane. Tilt the device to allow the fluid to collect/pool where the internal harvest line touches the base/side of the vessel.

FAQ

• Q: Can I use the GatheRex with external compressed gas sources?
  A: No, you should not attach the GatheRex to any external compressed gas source.

• Q: Can I sterilize the GatheRex?
  A: No, you should not sterilize the GatheRex as it may cause damage or distortion to the equipment.

• Q: Are these instructions applicable to all cell culture protocols?
  A: No, these instructions should not be considered as a comprehensive reference for all cell culture protocols.

• Q: What should I do if the product or packaging is damaged?
  A: Do not use the product if there is damage to the product or packaging that may have occurred during shipping or storage.

• Q: Can I open the equipment cabinet?
  A: No, opening the equipment cabinet can be dangerous and may cause electrical shock.

PRODUCT DESCRIPTION

The GatheRex™ Cell Harvest Pump is a liquid handling device designed to work with closed-system cell culture devices. The GatheRex connects to closed system devices and uses a low-pressure forced air mechanism to remove media, and concentrate cells and minimize storage and/or downstream processing. An internal air pump pressurizes the system by pumping air through the sterile vent filter on the cell culture device. When pressurized, excess media, or a cell fraction, is forced out of the device through the tubing assembly and then into a collection receptacle.

• Model 80000E is designated by white ID tags on the tubing pinch clamps. This version is compatible with small-diameter tubing (0.160” OD; blood transfer bag style) for both media removal and cell recovery.
• Model 80000Z is designated by black ID tags on the tubing pinch clamps. This version is compatible with large-diameter tubing (0.375” OD) for media removal and small-diameter tubing (0.160” OD blood transfer bag style) for cell recovery.

GatheRex Model| ID Tag Color| Media Removal – Tubing Diameter/Slot Width| Cell Recovery – Tubing Diameter/Slot Width
---|---|---|---
80000E| White| 0.160 inch| 0.160 inch
80000Z| Black| 0.375 inch| 0.160 inch

WARNINGS

1. The GatheRex is NOT a peristaltic pump. It is a positive pressure air pump regulated to reach and maintain a set pressure within a closed system G-Rex cell culture product in order to move cell culture media and cells out.
2. DO NOT consider these Instructions to be a comprehensive reference for the application of use in all cell culture protocols.
3. DO NOT use if there is damage to the product or packaging that may have occurred during shipping or storage.
4. DO NOT open the equipment cabinet. The danger of electrical shock.

PRECAUTIONS

1. DO NOT sterilize this product. Equipment may be damaged or distorted.
2. DO NOT immerse or overly wet the product during cleaning.
3. DO NOT attach the GatheRex to any external compressed gas source.
4. DO NOT position the equipment too closely to the side wall or equipment on its left or right. Allow space to the left and right of GatheRex.

CONTENTS

1. GatheRex Device
2. Tubing Assembly – for Low-Pressure Outlet Port
3. Power Cord

ELECTRICAL REQUIREMENTS

• Input: 100-240VAC, ~1.3A, 50-60Hz, Single Phase
• Fuse Type: 5ET 3.15-R, Cartridge, Time Delay, 3.15A, 250 V, 5mm x 20mm (0.2” x 0.79”), 5ET Series
• Detachable Power Cord: C13, 18AWG, 10A minimum, Grounded (3 Prong), UL 62, CSA-C22.2 No. 49
• IEC/UL: 61010-1 edition 3.1 and 61326-1:2012

LOW-PRESSURE OUTPUT

1. Gauge Pressure: 120 – 180 mmHg (2.32 – 3.48 psi) No adjustments for altitude are needed.
2. Flow Rate: Minimum 5 mL/sec fluid transfer rate from G-Rex M-CS

ENVIRONMENTAL CONDITIONS

1. Temperature: 15°C to 35°C (59-95°F)
2. Humidity: ≤ 95% Relative Humidity
3. Altitude: < 2,000 meters (6,561 feet)

SET UP

1. Set the GatheRex on a stable, level surface and plug-in power cord.
2. Place the closed system cell culture device to the left of the GatheRex.
3. Gather your desired culture media removal and cell recovery collection vessels.
   • Using a sterile tube welder, connect your collection vessels to the proper tubing of the cell culture device.
   • Alternatively, use the aseptic technique and the luer or quick-connect fittings to dock your collection vessels to the proper tubing of the cell culture device.
4. Attach the provided Tubing Assembly to the GatheRex compressed air outlet port. Note that the GatheRex is the source of compressed air.
5. Fasten the other end of the Tubing Assembly to the outlet of the sterile air vent filter of the cell culture device by way of a luer connection.
6. Position the Media Removal tubing into the GatheRex red clamp and the Cell Recovery tubing into the blue clamp.
   • Open the covers of the pinch valve/sensor.
   • Seat the tubing along the channel as shown.
   • Close the covers.
   • Run the tubing through the holding pins to keep them in position.
   • Note: Large-diameter C-Flex tubing will not fit into the holding pins.
   • Place the collection vessels to the right of the GatheRex.
7. Turn on the GatheRex by flipping the switch with the power cord plug-in.

EXAMPLE OF USE – Media Reduction and Cell Harvesting from a closed system G-Rex device

1. Ensure the pinch clamps for the selected fluid pathways are open to the media and cell collection bags.
2. Press the RED Right Arrow (à) to begin Media Removal (Reduction) through the red striped tubing or the large diameter CFlex tubing. The headspace of a closed system G-Rex device will be pressurized moving waste media to the collection bag. This will continue until the cell culture media level reaches the end of the internal media reduction line (~1 cm from the bottom). Once media removal from the G-Rex device has been achieved, media flow will stop automatically via a sensor located within the red tubing clamp.
3. Swirl the remaining volume in the G-Rex device to resuspend the cells existing on the gas-permeable membrane. Tilt the device to allow the fluid to collect/pool where the internal harvest line touches the base/side of the vessel.
4. With G-Rex still tilted, press the BLUE Right Arrow ( – >) to begin Cell Recovery (Harvest). The G-Rex will be pressurized moving the cell population and all remaining fluid into a Cell Harvest Bag. Media flow will stop automatically via a sensor located within the blue tubing clamp.
5. Optional Steps to harvest any potential remaining cells in the GRex cell culture device by way of a rinse procedure:
   • Partially refill the G-Rex with Media from the Red Removal Circuit by pressing the RED Left Arrow (ß) button. This releases the clamp for the red-striped removal conduit, allowing fluid to be gravity-fed or forced back into the G-Rex by squeezing the waste media bag manually or with a pressure sleeve.
   • When the desired volume of media has been moved back into the G-Rex, press the RED STOP (X) button to re-clamp the red-striped removal conduit.
   • Swirl the fluid within the G-Rex to capture and resuspend any remaining cells.
   • With the device tilted towards the internal harvest line, press the BLUE Right Arrow ( – >) button for cell harvest. Media flow will stop automatically via a sensor located within the blue tubing clamp.
6. Clamp the reduction line and the harvest line to seal the waste media collection bag and cell collection bag.
   • Alternatively, simply seal the collection bags with a sterile tube sealer.
7. Press the release clamps on the GatheRex Pump and remove the tubing.
8. Seal the collection bags with a sterile tube sealer.
9. Discard the G-Rex vessel and the waste culture media.
10. See examples of setup with G-Rex at the end of this document.

MAINTENANCE AND SERVICE

• Maintenance – There are no maintenance items on the GatheRex other than periodically wiping down the exterior of the device with 70/30 IPA.
  • Use care not to over-saturate or immerse the device.
  • If the power cord becomes damaged contact Wilson Wolf for a replacement.
  • All repairs and services must be performed by Wilson Wolf.
• Inspection – Pressure output should be verified before each use using an external calibrated pressure gauge (see air pressure verification details below).
• Air Pressure (verification) – Note that the internal pressure regulator cannot be adjusted (i.e., output pressure can only be verified). To measure pressure output, connect a calibrated pressure gauge to the compressed air outlet port and tubing. An appropriately sized fluid-filled tube must also be placed into the pinch clamp/sensor to allow the pump to run for more than 6 seconds once initiated using the – > key.
• Fuse Replacement – Unplug the cord from the wall outlet. Remove the Cord from the Power Module on the back of GatheRex. Pry open the top of the access door of the Power Module with a flat screwdriver. Use a flat screwdriver to pry the side of the Fuse Carrier for removal. Inspect and replace fuse (see page 1 for fuse type). Re-insert the Fuse Module into GatheRex with the appropriate voltage positioned at the top (i.e., 115V or 230V). Close the Power Module door and verify the desired voltage is shown through a window in the Power Module door. Re-install the Power Cord and plug it into a wall outlet.

TROUBLESHOOTING

• If Flashing Red Power LED – If the system is unable to build pressure (i.e., system leak) the GatheRex will run for approximately 33 seconds and then stop the air pump. The red power LED will flash red. To reset, turn the device off and then back on again. Fix the system leak before restarting the cycle.
• If the GatheRex Air Pump will not run longer than 6 seconds – Verify that the tubing is fully seated in the pinch clamp and that fluid fills the tubing within 6 seconds. If air is present in tubing the pump will stop.
• If Green Power LED will not light – Confirm outlet has power. Verify that the power cable is fully seated in the outlet and back of the device. Confirm GatheRex power switch is turned ON. Inspect and/or replace the Fuse per Maintenance and Service instructions.

EXAMPLE OF SETUP – GatheRex 80000E and G-Rex100M-CS:

EXAMPLE OF SETUP – GatheRex 80000Z and G-Rex500M-CS:

MANUFACTURED BY
Wilson Wolf Manufacturing Corporation St. Paul, Minnesota 55112 USA Made in USA.

TECHNICAL SUPPORT/ORDERING

• Tel (+1) 651.628.9259
• Email info@wilsonwolf.com.
• Web www.wilsonwolf.com.

Patent: www.wilsonwolf.com/patents

G-Rex® is a registered trademark of Wilson Wolf Corporation GatheRex™ is a trademark of Wilson Wolf Corporation © 2020 Wilson Wolf Corporation. All Rights Reserved.

References

• Wilson Wolf Manufacturing
• patents | Wilson Wolf Manufacturing
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