opentrons Heater-Shaker Module GEN1 Instruction Manual

opentrons Heater-Shaker Module GEN1

Product and Manufacturer Description

PRODUCT DESCRIPTION

• The Opentrons Heater-Shaker Module GEN1 provides on-deck heating and orbital shaking. The module can be heated to 95 °C, and can shake samples from 200 to 3000 rpm.

MANUFACTURER DESCRIPTION

• Opentrons Labworks Inc
• 45-18 Ct Square W
• Long Island City, NY 11101

Safety Information and Regulatory Compliance

• Opentrons strongly recommends that you follow the safe use specifications listed in this section and throughout this manual.
• The shaking motion of the Heater-Shaker requires additional anchoring – more than simply clipping it into a deck slot – to ensure that it does not dislodge itself from the deck of your robot. See the Setup Steps section of this manual or the on- screen instructions in the Opentrons App for details on how to anchor the module.

SAFE USE SPECIFICATIONS

Input and Output Connections

• The Heater-Shaker has the following power input and output requirements, which are met by the included power supply brick.
• Warning: Do not replace the power supply cord unless at the direction of Opentrons Support.

Power Supply (AC):

• Voltage: 100-240 V
• Frequency: 50/60 Hz
• Current: 4.0 A
• Mains supply voltage fluctuation: #10%
  Overvoltage: Category II

Heater-Shaker (DC):

• Voltage: 36 V
• Current: 6.1 A
• These input and output requirements are also indicated on the external power adapter.

Environmental Conditions

• For optimal performance, Opentrons recommends using the
• Heater-Shaker in the following conditions:
• Environment: Indoor use only
• Temperature: 20-25 °C
• Relative humidity: 80% maximum, non-condensing
• Altitude: Up to 2,000 meters above sea level
• Pollution degree: 2
• The Heater-Shaker can be safely transported and stored at temperatures of -10 to 60 °C.

Software Requirements

• The Heater-Shaker requires version 6.1.0 or newer of the Opentrons
• App and OT-2 robot server.
• You can download the Opentrons App for Windows, macOS, or Ubuntu at https://opentrons.com/ot-appl.

LED STATUS LIGHT

• The Heater-Shaker has an LED status light that can display three colors: amber, red, and white. Reading these status indications is important for safe handling of the module (see Temperature Safety below).
• The possible module conditions indicated by the light are:

and/or

Module is hot to the touch (>49 °C)

Solid white| Module is on and idle

and/or

Module has been deactivated and it is not hot to the touch (<49 °C)

Pulsing white| Module is in identify mode (to distinguish between

multiple Heater-Shaker Modules)

Pulsing amber| Module is in an error state
Pulsing red and amber, alternating| Module is in an error state

and

Module is hot to the touch (>49 °C)

Pulsing red and white, alternating| Module is in identify mode

and

Module is heating

LED off| Module is powered off

Temperature Safety

• During normal operation, the top plate, labware adapters, and labware on top of the Heater-Shaker can reach temperatures of up to 95 °C. Touching these surfaces while they are hot poses the risk of burns.
• To reduce the risk of burns, always check the color of the status light before touching the module. If the status light is red, the module may be hot to the touch. Do not touch the module. Use the Opentrons App to deactivate the heater and allow the module to cool until the status light is solid white.
• If you must touch a module that is powered off, and you are unsure whether it is hot to the touch:
• If you can do so without touching any other part of the module, press the power button to activate the module and check the color of the status light.
• If you cannot safely press the power button, allow the module to cool for at least 10 minutes before touching it.

Powering Down

• When powering down the Heater-Shaker Module, ensure that it is idle (not shaking) and not hot to the touch by checking that its status light is solid white. If the status light is red, the module is hot; allow the module to cool until the status light is white before powering it down.
• Powering down the module while the status light is red prevents other users from determining whether the module is hot to the touch.
• Press the power button above the USB connector to turn off the module. You can unplug the power adapter once the module is powered down.

SAFETY WARNING LABELS

• Warning labels on the Heater-Shaker indicate sources of potential injury or harm.
• Hot   Surface: This symbol identifies instrument components that, if handled improperly, pose a risk of personal injury due to high temperature (>49 °C).

STANDARDS COMPLIANCE

• The Heater-Shaker has been tested and found to be in compliance with all applicable requirements of the following safety and electromagnetic standards:

Safety

• IEC/EN 61010-1 Safety Requirements for Electrical Equipment for
• Measurement, Control and Laboratory Use
• IEC 61010-2-010 Requirement for Heating
• IEC 61010-2-051:2018 Particular requirements for laboratory equipment for mixing and stirring

Electromagnetic Compatibility

• FCC part 15 subpart B class A
• IEC/EN 61326-1 EMC Testing of Laboratory Equipment
• Hazardous Substances: RoHS compliant

FCC Warnings and Notes

• Warning: Changes or modifications to this unit not expressly approved by Opentrons Labworks Inc. could void the user’s authority to operate the equipment.
• Note: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC Rules.
• These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense.
• Note regarding FCC compliance: Although this instrument has been tested and found to comply with Part 15, Subpart B of the FCC Rules for a Class A digital device, please note that this compliance is voluntary, for the instrument qualifies as an “exempted device” under 47 CFR 15.103(c), in regard to the cited FCC regulations in effect at the time of manufacture.

CISPR 11 Class A

• Caution: This equipment is not intended for use in residential environments and may not provide adequate protection to radio reception in such environments.

Product Specifications

• MODEL NUMBER GEN1

INCLUDED PARTS

• (1) Power Supply
• (1) Power Cable
• (1) USB Cable
• (1) T10 Torx Screwdriver
• (1) T10 Torx L Key
• (1) M3x7 Thermal Adapter Screw (plus spares)

THERMAL ADAPTERS

• A compatible thermal adapter is required for adding labware to the
• Heater-Shaker Module. Adapters can be purchased directly from
• Opentrons at https://shop.opentrons.com.
• Currently available Thermal Adapters include:
• Universal Flat Adapter
• PCR Adapter
• Deep Well Adapter
• 96 Flat Bottom Adapter

PHYSICAL SPECIFICATIONS

• All specifications are for the module as shipped: with latches in the rest position and without labware or adapters.
• Dimensions: 152 mm L × 90 mm W x 82 mm H
• Weight: 1.34 kg
• Composition: CNC aluminum and polycarbonate plastic

POWER CONSUMPTION

• These are typical power consumption values measured at the wall outlet. Typical power consumption is when the module is maintaining temperatures and shake speeds in the normal range.
• The maximum power consumption is when the module is simultaneously heating at full power and shaking at maximum speed.
• Idle: 3 W

Typical consumption:

• Shaking: 4-11 W
• Heating: 10-30 W
• Heating and shaking: 10-40 W
• Maximum consumption: 125-130 W

SHAKING PROFILE

• Orbital diameter: 2.0 mm
• Orbital direction: Clockwise
• Speed range: 200-3000 pm (some labware may recommend a lower maximum rpm)
• Speed accuracy: ‡25 rpm

TEMPERATURE PROFILE

• Temperature range: 37-95 °C
• Temperature accuracy: $0.5 °C at 55 °C
• Temperature uniformity: $0.5 °C at 55 °C
• Ramp rate: 10 °C/min

LIQUID CLASSES AND VOLUMES

• For further information, consult the most recent application notes and white papers available on the Heater-Shaker product page on the Opentrons website.

SUPPORTED LABWARE

• NEST 96 Well, 0. 1 mL, Round Well, V Bottom, Full Skirt
• NEST 96 Well, 0.2mL, Round Well, Flat Bottom, Full Skirt
• NEST 96 Well, Deep Well, 2mL, Square Well, V Bottom, Full Skirt
• Thermo Scientific Armadillo PCR Plate, 96-Well, Clear, Clear Wells
• Corning 384 Well Plate 112 L Flat
• Custom flat-bottom labware can be used with the Universal Flat Adapter. If you need assistance creating custom labware definitions for the Heater-Shaker, submit a request.

Setup and Maintenance

IN-APP SETUP GUIDE

• For interactive setup instructions and to run a test shake with your Heater-Shaker Module, connect the module to your robot (see Connecting USB & Power below) and either:
• Go to the Pipettes and Modules section on the robot’s device detail page.
• Load a protocol that requires the module and go to Step 2: Module Setup in the Setup tab of the protocol run page.

SETUP STEPS

Preparing the Module

1. Orient the module so its ports face away from you.
2. Make sure the anchors are not extended and are level with the module’s base. Turn the screws above each anchor counterclockwise to retract the anchors. The screws should not come out of the module.
   • Attaching to Deck
3. Choose which slot you will place the Heater-Shaker in. Slots 1, 3, 4, 6, 7, and 10 are supported.
4. Place the module in the slot such that the power and USB ports are facing outward, i.e. to the left in slot 1, 4, 7, or 10; to the right in slot 3 or 6.
5. Hold the module flat against the deck and turn the screws clockwise to extend the anchors.
6. Check that the module is firmly attached by gently pulling up on it and rocking it from side to side.
   • Attaching a Thermal Adapter
7. Use the included T10 Tor Screwdriver and Thermal Adapter Screw to attach your chosen adapter to the module.
   • IMPORTANT: Using a different screwdriver can strip the screws. Using different screws can damage the module.
8. Check the alignment of the thermal adapter.
   • If properly attached, it will sit evenly on the module.
9. Check that the adapter is firmly attached by rocking it back and forth.
   • Connecting USB & Power
10. The module requires two connections:
11. Connect the power cable to the power supply and the module. Route the cable through the gap at the bottom of the robot’s side window.
12. Connect the USB cable to the module and an open USB port at the back of the robot. Use the cable holders on the side of the robot to keep the USB cable out of the way.
13. Power on the robot and module, and launch the Opentrons App.
    • If you successfully connected your module, it will appear in the Pipettes and Modules section on your robot’s device detail page. From there you can control the module’s labware latch or run a test shake.

SOFTWARE CONTROL

• The Heater-Shaker Module can be controlled in JSON (Protocol Designer) and Python protocols. Running these protocols requires version 6.1.0 or newer of the Opentrons App and OT-2 robot server.
• The Opentrons App displays the current status of the Heater- Shaker and can also control the module outside of protocols.
• To control a Heater-Shaker, go to the Devices tab and select the robot that it is connected to. Robots with a connected and powered on Heater-Shaker will have a * icon under the Modules section of the device card.
• On the device detail page, a module card shows the current status of the Heater-Shaker, including:
• Whether the heater is heating, cooling, or holding at target.
• warning banner will appear if the module is hot to the touch (>49 °C).
• The target (if set) and current temperature
• Whether the shaker is speeding up, slowing down, or holding at target
• The target (if set) and current shake speed
• Whether the labware latch is open or closed
• Click the three-dot menu to reveal the available controls for the Heater-Shaker. You can control the heater, shaker, and labware latch independently. You can also view information about the module, including its serial number and firmware version, or launch the in-app attachment guide.

MAINTENANCE

Device Lifetime

• The Heater-Shaker has been tested for 3 years of full-time operation (more than 1000 total hours) without maintenance.

Service and Repair

• Users should not attempt to service or repair the module themselves.
• If you have concerns about your module’s performance or require service, please contact Opentrons Support.

Cleaning

• Before cleaning the Heater-Shaker, unplug the power and USB cables and remove it from the deck.
• All outer surfaces of the Heater-Shaker are compatible with:
• 10% bleach solution
• 70% ethanol
• 99% isopropyl alcohol
• Spray one of these cleaners onto a paper towel and use it to wipe the exterior surfaces of the module. Avoid introducing these substances into the USB port or power port. Never douse or spray the module directly with cleaner.
• The module can also withstand ultraviolet (UV) light, limited to standard sterilization procedures. Do not expose the module to constant or high-intensity UV light.

Support OPENTRONS HELP CENTER

• Answers to many common questions are available on the Opentrons
• Help Center at http://support.opentrons.com/.

CONTACT OPENTRONS

• If you have any other questions, contact [email protected].
• Please have the serial numbers of your module and robot available when contacting support.

WARRANTY

• All hardware purchased from Opentrons is covered under a 1-year standard warranty.
• Opentrons warrants to the end-user of the products that they will be free of manufacturing defects due to part quality issues or poor workmanship and also warrants that the products will materially conform to Opentrons’ published specifications.

References

• Opentrons Help Center
• Opentrons Protocol Designer BETA
• Hardware Modules — Opentrons Python API V2 Documentation
• Heater-Shaker Module | Opentrons OT-2 Modular Hardware | Opentrons
• Opentrons App - Opentrons
• Opentrons - Automated Pipetting Robots for Life Science Labs
• Opentrons Help Center

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