Total Canine Care FM15.30140.04_OC Giardia Test for Dogs Instructions

Total Canine Care FM15.30140.04_OC Giardia Test for Dogs

Product Information

The Total Canine CareTM Giardia lamblia Rapid Test Cassette (feces) is a lateral flow immunochromatographic assay for the qualitative detection of Giardia lamblia antigen in animals’ feces. It can detect both trophozoite and cysts stages of giardia in the feces. The kit is manufactured by Hanghou All Test Biotech Co., Ltd and distributed by MR Diagnostic Services LLC.

Product Usage Instructions

1. Step 1: Collect sufficient quantity of feces (1-2 mL or 1-2 grams) in a clean container.
2. Step 2: Hold the dropper vertically, aspirate fecal specimens, and transfer 2 drops of the liquid specimen (approximately 80 uL) into the specimen collection tube containing the extraction buffer. Tighten the cap onto the specimen collection tube and shake vigorously to mix the specimen and the extraction buffer. Allow the collection tube to react for 2 minutes.
3. Step 3: Bring the pouch to room temperature before opening it. Remove the test cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the test is performed immediately after opening the foil pouch.
4. Step 4: Hold the specimen collection tube upright and unscrew the tip of the specimen collection tube. Invert the specimen collection tube and transfer 2 full drops of the extracted specimen (approximately 80 uL) to each specimen well (S) of the test cassette. Start the timer and avoid trapping air bubbles in the specimen well (S).
5. Step 5: Read the results at 10 minutes after dispensing the specimen. Do not read results after 20 minutes.

Reminder: This test is an acute-phase screening test, and all results should be combined with clinical information obtained by veterinarians. For further information or support, you can contact Hanghou All Test Biotech Co., Ltd or MR Diagnostic Services LLC.

Contents

• 10X Dual-Test Cassette in Foil Pouches (w/ Desiccant & Disposable Dropper)
• 10X Test Assay Buffer/Dropper Tubes
• 1X Biohazard Collection Bag
• 1X User Guide

Complementary Items

• Reusable Feces Collection Tube (w/ touch-free spoon)
• Travel Tube Disinfectant Alcohol Wipes
• Nitrile Gloves

Intended Use

Total Canine Care™ Giardia lamblia Rapid Test Cassette (feces) is a lateral flow immunochromatographic assay for the qualitative detection of Giardia lamblia antigen (two subtypes: trophozoite and cysts in animals feces.
In the life history of giardia, there are two different stages of development: trophozoite and cysts. Generally speaking, cysts exists in formed feces and trophozoite exists in watery feces with diarrhea. This kit can detect giardia in both trophozoite and cystst stages. Therefore, it is always recommended to use both testing wells to ensure thorough review and comprehensive screening.

Test Principle

Giardia lamblia Rapid Test Cassette is a lateral flow immunechromatographic assay based on sandwich format. The test cassette has two testing windows. During testing, sample is applied into the sample wells on the cassette. Giardia antigens, if present in the specimen, react with anti-Giardia antibodies coated colloidal gold particles in the test strip. The mixture then migrates upward on the membrane chromatographically by capillary action and reacts with anti-Giardia antibodies on the membrane in the test line region. If the specimen contains Giardia antigen, a colored line will appear in the test line region indicating a positive result. If the specimen does not contain Giardia antigens, a colored line will not appear in the test line region indicating a negative result. To serve as a procedural control, a colored line will always appear in the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.

Warning Symbols

Index of Warning Symbols

Precautions

• For animal use only. Not for use in humans. Keep out of reach of children.
• This kit is designed for in vitro veterinary diagnosis use only.
• Follow warning symbol recommendations.
• Handle all specimens as if they contain infections agents. observe established precautions against microbiological hazards through testing and follow the stand procedures for proper disposal of specimens.
• Wear disposable gloves and eye protection when specimens are being tested.
• DO NOT use after expiration date.
• DO NOT mix components from different lots and different products.
• DO NOT operate near heavy vibrations or on steep inclines.
• DO NOT operate in dusty conditions.
• DO NOT touch the sample deposit area of the test cassette.

Recommended Materials

• Timer
  • Proper testing requires: 2, 10, and 20 minute intervals.

Limitation of Test

• This is an acute-phase screening test. All results should be combined with clinical information obtained by veterinarians.

Directions for Use

Step 1

Collect sufficient quantity of feces (1-2 mL or 1-2 grams) in a clean dry specimen collection container to obtain enough pathogens. Best results will be obtained if the assay is performed within 5 hours after collection. Specimen collection may be stored for 3 hours after collection. Specimen collection maybe stored for 3 days at 2-8°C if not tested within 5 hours. For long term storages, specimens should be kept below -20°C.

Step 1a: SOLID Specimens

Unscrew the green cap of the buffer tube, then selectively poke the white collection applicator into the fecal specimen in at least 3 different sites to collect approximately 50 mg of feces (equivalent to ¼ of a pea). Do not scoop the fecal specimen with the applicator due to possible dirt contamination.

Step 1b: LIQUID Specimens

• Hold the dropper vertically, aspirate fecal specimens, and then transfer 2 drops of the liquid specimen (approximately 80 uL) into the specimen collection tube container the extraction buffer.

Step 2: Tighten the cap onto the specimen collection tube, then shake the specimen collection tube vigorously to mix the specimen and the extraction buffer. Allow the collection tube to react for 2 minutes.

Step 3: Bring the pouch to room temperature before opening it. Remove the test cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the test is performed immediately after opening the foil pouch.

Step 4

Hold the specimen collection tube upright and unscrew the tip of the specimen collection tube. Invert the specimen collection tube and transfer 2 full drops of the extracted specimen (approximately 80 uL) to each specimen well (S) of the test cassette, then start the timer. Avoid trapping air bubbles in the specimen well (S).

Step 5

Read the results at 10 minutes after dispensing the specimen. Do not read results after 20 minutes. (Optional) If the specimen does not migrate (due to the presence of particles, centrifuge the diluted sample contained in the extraction buffer vial. Collect 160 uL of supernatant, dispense into each specimen well (S) of a new test cassette and proceed with all Steps 1-5 again. Dispose of used test cassettes and buffers in provided biohazard bags. Bag. Please consult local phone or internet directories for the correct information and disposal guidelines.

Interpretation of Results

• Hold Read the results at 10 minutes after dispensing the specimen.
• Do not read results after 20 minutes.

INVALID Test

POSITIVE Test

NEGATIVE Test

REMINDER: This is an acute-phase screening test. All results should be combined with clinical information obtained by veterinarians.

Step 4a: Re-Order by Scanning the QR Code

Step 4b: Re-Order by Downloading the App

Step 4c: OPTIONAL Re-Order by Visiting our Website

• WWW.CANINEP4.COM

Warranty Disclaimer

For animals only. Not for use in humans. Keep out of reach of children. Intended for veterinary use only. Do not freeze. Store at room temperature. Keep out of direct or artificial sunlight. User (or Purchaser) is responsible to observe all applicable local, state, or federal law and bear all risks therefor. User shall dispose of all sharps in authorized waste containers, as identified by User’ s local trash removal services or health departments. Please consult local phone or internet directories for the correct information and disposal guidelines. Do not dispose sharps in standard trash. MRDS, as Distributor, warrants the kit for its intended veterinary product use and will replace any defect product purchased by User upon MRDS’ receipt of timely written notice by User. This shall be Users exclusive remedy and User shall not be entitled to additional or further compensation or cost.

MRDS is not responsible for pre-existing conditions (even if manifest during use of the products), abnormal response to product use not caused by defect product including but not limited to, aggression, anxiety, and destructive and/or compulsive behavior. MRDS is not responsible if death or euthanasia occurs in your pet because of illness, injury or because of behavior or emotional disorder, including stress induced ovulation losses. Total Canine Care™ is a trademark of MR Diagnostic Services LLC. Any use or misrepresentation without the prior written permission is strictly prohibited.

Copyright © 2023 by MR Diagnostic Services LLC

All rights reserved. No part of this publication may be reproduced, distributed, or transmitted in any form by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of MRDS. The information in this document is correct at the time of printing. However, MRDS reserves the right to make any necessary changes at any time without notice as our policy is one of continuous improvement.

Contact

Manufactured By

• Hanghou All Test Biotech Co., Ltd 550 Yinhai Street, Hangzhou Economic & Technologic Development Area Hangzhou, 310019, P.R China

Distributed By

• MR Diagnostic Services LLC
• Address: 5900 77th Street Kenosha, WI 53142
• Email: support@K9P4go.com
• Website: www.caninep4.com

FM15.30140.04

References

• Progesterone Test for Dogs at Home , Test kits, and Supplies

Read User Manual Online (PDF format)

Read User Manual Online (PDF format)  >>

Download This Manual (PDF format)

Download this manual  >>