GridION GRD-MK1 Sequencing Device User Guide

GridION GRD-MK1 Sequencing Device

Pre-installation

This Quick start guide contains everything you need to set up your GridION™ and to check that the device is ready for use.

GridION user manual
community.nanoporetech.com/to/gridion

Safety and regulatory information
community.nanoporetech.com/to/safety

For detailed information and troubleshooting, view the user manual.
*The GridION Mk1 ships with 5 x power cables (1 US, 1 UK, 1 EU, 1 CN, 1 AUS) for international use.

What’s in the box

Set up your device

1. Unpack your GridION device*.
2. Attach the cables and peripherals as shown opposite.
3. Connect the power supply.
4. Press the power button.

Rear input/output

Only use the ports and connections coloured in blue for installation.

• Place device on a well-supported, strong, clean bench. Allow 30 cm clearance rear and sides, and do not cover ventilation grilles. See user manual for detailed installation advice.
  † If using a HDMI-only monitor, use the included DisplayPort-to-HDMI adapter.

Log in to MinKNOW™

1. Log in to your GridION Password: grid.

2. Open MinKNOW
   Click the wheel icon on the desktop to load MinKNOW, the device operating software.

3. Log in to MinKNOW
   Use your Oxford Nanopore account details.

Note : follow the pop-up tutorials in MinKNOW to familiarise yourself with the software.

Update software

For the latest sequencing features, update MinKNOW:

Power off the device (step 4).

Power off

Follow the workflow below to correctly power off your device:

When powering on the device again, wait 10 seconds before pressing the power button.
Repeat step 2 (Log in to MinKNOW) and then proceed to step 5 (Perform a hardware check).

Oxford Nanopore Technologies
phone +44 (0)845 034 7900
email support@nanoporetech.com
@nanopore

www.nanoporetech.com
Oxford Nanopore Technologies, the Wheel icon, MinKNOW, and GridION are registered trademarks of Oxford Nanopore Technologies plc in various countries. All other brands and names contained are the property of their respective owners. © 2024 Oxford Nanopore Technologies plc. All rights reserved. Oxford Nanopore Technologies products are not intended for use for health assessment or to diagnose, treat, mitigate, cure, or prevent any disease or condition.
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Perform a hardware check

A hardware check is required before performing your first GridION sequencing run. To run a hardware check, follow the on-screen instructions in MinKNOW, then follow the instructions below. You will require your five GridION Configuration Test Cells (CTCs).

Hardware check overview:

1. Insert the CTCs into the device as shown and close the lid of the device.
2. In the MinKNOW software, the flow cell status indicators (the five boxes) will change colour from grey to white.
3. Press Select all available. This will change the colour of the flow cell status indicators (the five boxes) on the MinKNOW hardware check panel to dark blue.
4. Press Start in the bottom right.
5. Check the flow cell positions show a to pass the hardware check.
6. Remove the CTCs from the flow cell positions after you complete the hardware check.

Note : If your hardware check fails, see Support in the Additional information section.

Discover the Nanopore Community

community.nanoporetech.com

Ensure the success of your nanopore sequencing project and stay up-to-date with the latest technology and protocol updates.

Tip: Learn how to analyse your nanopore data at: nanoporetech.com/analyse

Additional Information

• Warranty
  A license and warranty can be purchased for your device here: store.nanoporetech.com/device-warranty.html
  Flow cell warranty: community.nanoporetech.com/to/warranty

• Recycle used flow cells
  Oxford Nanopore is committed to environmental sustainability.
  You can help by sending your flow cells for recycling.
  Find out how: community.nanoporetech.com/support/returns

• Place your next order
  Buy more consumables at the Oxford Nanopore Store: store.nanoporetech.com

• Documentation
  Documentation for your device is available on the Nanopore Community: community.nanoporetech.com/docs

• Support
  For all of your customer and technical support needs, visit: community.nanoporetech.com/support

Technical specification

1 x HDMI/DisplayPort to monitor 1 x USB for keyboard

| 1 x USB for mouse 1 x Power socket
Software installed| Ubuntu, GridION OS, MinKNOW
Compute specification| 7 TB SSD storage, 64 GB RAM, minimum 8 core Intel CPU, 1 x Nvidia GV100
Environmental conditions| Functional range of electronics is within environmental temperatures of +5°C to +40°C. Users should allow 30 cm clearance to the rear and sides of the device.

Designed to sequence in environmental temperatures of +18°C to +25°C. Intended for indoor use.

Can be used up to altitudes of 2,000 m.

Use within 30%–75% relative non-condensing humidity limits. The device has a Pollution Degree 2.

WARNING: Rear of instrument heats up during operation.

References

• community.nanoporetech.com/docs
• nanoporetech-customers - Sign In
• Community
• Community
• Oxford Nanopore technologies data analysis solutions
• store.nanoporetech.com
• store.nanoporetech.com/device-warranty.html
• Welcome to Oxford Nanopore Technologies

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