greiner 655846 96-Well Bioassay Kit Instruction Manual

greiner 655846 96-Well Bioassay Kit Instruction Manual

96-WELL BIOASSAY KIT
Item No.: 655846

Generating and handling of dot and ring 3D cell culture structures by using magnetic forces.

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INTRODUCTION:

Thank you for purchasing this Greiner Bio-One product.
The 96-Well BioAssay Kit uses NanoShuttle-PL, a nanoparticle assembly consisting of gold, iron oxide, and poly-L-lysine to magnetise cells. In this kit, cells in a 6 and 96 well cell-repellent microplate are magnetically levitated and bioprinted to assemble/aggregate cells into 3D cultures shaped as dots or rings.

BASIC APPLICATION OF THIS TECHNOLOGY:

• Create 3D cell cultures in a fast and easy workflow
• Analyze 3D cell cultures using common biological research techniques, such as biochemical assays e.g. in drug or toxicity screenings
• Easy handling, without the risk of sample loss
•  Bioprinting of spheroids or 3D ring structures
• Wound healing assay
• Cell migration

The magnets in the kit are strong, can damage electronics, and causes injury if not handled properly. Read the safety precautions carefully to ensure the correct handling of the magnets.

SAFETY PRECAUTIONS:

To guarantee problem free and safe operation of the magnetic 3D cell culture kit please read these safety precautions before using.

• The magnet plates contain strong neodymium magnets that must be handled with extreme care.
• When storing magnets in proximity to other magnets or materials that are attracted to magnets, take precautions so that objects do not slam together. Neodymium magnets are brittle and can shatter or crack, sometimes producing dangerous fragments moving at high speeds. Fingers can also be severely pinched between magnets or between magnets and certain metals. Large magnets can be difficult to separate from other magnets or certain metals if they come into contact.
• DO NOT remove the magnets from the protective covers.
• Keep the magnetic drives spatially separated and DO NOT put the drives together at any time. Due to the magnetic force, placing them in close proximity can cause them to “crash” together, resulting in damage to the drive magnets and / or structure.
• Persons with pacemakers or similar medical devices should not come near Neodymium magnets.
• Neodymium magnets can damage magnetic media such as credit cards, magnetic ID cards, televisions, computer memory, and computer monitors. Keep magnets at least 30 cm (12 in.) from these devices away.
• Neodymium magnets should not be burned or machined. They will lose their magnetic properties if heated above 80 °C (175 °F). DO NOT AUTOCLAVE the magnetic drives.
• Neodymium magnets are not toys. The magnetic drives should only be used for their intended purpose of levitation or bioprinting cell culture. Children should not be allowed to play with them.
• Store the NanoShuttle-PL vials at 4 °C to 40 °C until first use. After first opening of the vial, the storage recommendation is 4 °C to 8 °C.
  DO NOT place the NanoShuttle-PL at temperatures below 0° C (at 1 atm) or below water freezing temperature.

INTENDED USE:

For magnetisation of cells to use in 3D cell culture. For research use only. Cell culture disposable to be used by trained personnel in a laboratory surrounding.

MATERIALS:

OVERVIEW:

INSTRUCTIONS:

INSTRUCTION TO TREAT CELLS WITH NANOSHUTTLE-PL AND HANDLING 3D CELL CULTURES

Note: Protocol optimization may be required for different cell types and/ or specific experimental aims.
Do not soak drives in ethanol. Lightly spray with ethanol and wipe to sterilise and DO NOT AUTOCLAVE the magnetic drives (see also safety precautions).

WARNING
Do not use both magnets at once, as the holding drive will tend to bring the cells to the bottom of the plate, with little to no effect from the levitation drive.

HANDLING BEYOND THE STANDARD PROTOCOL:

TROUBLESHOOTING:

CELL TYPES AND PUBLICATION:

Cell types that have been successfully cultured and publications using the technology are listed here.

A video showing the handling of the m3D BioAssay products and the experimental workflow is available here or by scanning the QR code.

WARRANTY:

The Greiner Bio-One magnet plates are warranted to be free of defects in material and workmanship for a period of 2 years from the date of purchase. The warranty is valid only if the product is used in its intended purpose and within the guidelines specified in this instruction manual. In the case that service or technical support is required, please contact your local subsidiary or Greiner Bio-One distributor.

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GREINER BIO-ONE GMBH
FRICKENHAUSEN, GERMANY
PHONE +49 7022 948-0
FAX +49 7022 948-514
E-MAIL info@de.gbo.com

GREINER BIO-ONE IS A GLOBAL PLAYER.
FIND THE CONTACT DETAILS OF YOUR
LOCAL PARTNER ON OUR WEBSITE.

Devices of Greiner Bio-One are to be used by properly qualified persons only in accordance with the relevant Instructions for Use (IFU), where applicable.
For more information contact your local Greiner Bio-One sales representative or visit our website (www.gbo.com).

All information is provided without guarantee despite careful processing. Any liability, warranty or guarantee of Greiner Bio-One GmbH is excluded. All rights, errors and changes are reserved. If not stated otherwise, Greiner Bio- One GmbH has all copyrights and/or other (user-)rights in this document, in particular to signs such as the mentioned (word-picture-)brands and logos. Any use, duplication or any other use of the rights of Greiner Bio-One GmbH is expressly prohibited.

Media owner: Greiner Bio-One GmbH / Represented by Managing Director Bernd Klingel.
The company is registered in the Commercial Register at the first instance court in Stuttgart, HRB 224604 / VAT Number: DE812585719.

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References

• Greiner Bio-One | Making a difference
• Greiner Bio-One | Making a difference
• 3D cell culture

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