BIOER GE-384G GeneExplorer Thermal Cycler

GeneExplorer Thermal Cycler

The GeneExplorer Thermal Cycler is a laboratory instrument manufactured by Hangzhou Bioer Technology Co., Ltd. It is available  in different models: GE- 96G, GE-384G, GE-4I, GE-48D, GE-48DS, GE-48DG, and GE-96S.

Important Notes

Convention

Please read the following important information before using the GeneExplorer Thermal Cycler

• Modification to the information contained herein may occur without further notice.
• The document contains patent information under copyright protection. All rights reserved.
• The Operation Manual should be carefully read before using the instrument for the first time.

Attention:| Information of extreme importance is contained under the item of Attention, thus please carefully read it. Failure to practice as per the

instruction may lead to equipment damage or operation failure.

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Warning!| Information under the item of Warning requires special attention for

specific operation step or method. Failure to practice as per the requirements may lead to severe personal injury.

1 Safety
The following basic safety measures must be abided by through all stages including equipment operation, maintenance and repair. Failure to abide by these measures or warnings indicated in the manual may affect the protection provided by the instrument. Meanwhile, it may damage the safety standard for design and manufacturing, and expected application scope of the instrument. Hangzhou Bioer Technology Co., Ltd. takes no responsibility for any consequences arising from user’s failure for abiding by the following requirements.

Ensure the following safety precautions when using the GeneExplorer Thermal Cycler:

1. Instrument grounding: The instrument must be reliably grounded to prevent electric shock. It uses a three-core grounding plug that requires a grounding socket.

2. Keep away from energized circuit: Do not dismantle the instrument without proper authorization. Component replacement or adjustment should be done by specialized maintenance personnel.

3. Attention for power supply: Before connecting AC power supply, ensure the voltage consistency and the rated loading of the power socket.

4. Attention for power line: Handle the power line with care to avoid damage.

5. Plug in and plug off: Hold the plug when plugging in or unplugging the instrument. Ensure proper insertion and avoid forcibly pulling the power line.

6. Attention for instrument placing: Do not install the equipment on inflammable materials.

7. Attention for operation: Take precautions to avoid liquid dripping on the instrument. Wear personal protective equipment, such as protective gloves, when handling reagents.

8. Instrument grounding To prevent electric shock, the input power line of the instrument must be reliably grounded. The instrument adopts three-core grounding plug which includes a third (grounding) foot and can only used together with grounding socket. This is a safety device. If the plug cannot be inserted into socket, please ask an electrician to install correct socket, and don’t let the grounding socket lose its function of safety.

9. Keep away from energized circuit Operator should not arbitrarily dismantle the instrument. Component replacement or in-machine adjustment must be completed by specialized maintenance personnel with certificate. Don’t replace any component when the power line is connected.

10. Attention for power supply Before connecting AC power supply, ensure the consistence of the voltage of the power supply and the voltage required by the instrument (a deviation of 10% is allowed), and ensure the rated loading of the power socket is no less than the requirement of the instrument.

11. Attention for power line Generally, the instrument should use the power line supplied along with the instrument. In case of power line damage, it must be replaced instead of repair. For replacement, power line of same type and specification should be used. Don’t put anything on the power line when the instrument is being used. Don’t place the power line in the place where people walk through. If the power line will contact thermal surface when it is used, then protection measures should be taken to prevent insulation damage to the power line.

12. Plug in and plug off The plug must be held in hand for plugging in and plugging off. For plugging in, ensure that the plug is completely inserted into the socket, and don’t pull the power line forcibly for plugging off.

13. Attention for instrument placing The instrument should be placed in area of low humidity, less dust and far away from water source (such as close to water pool or water pipe), and the place should be well ventilated and free from corrosive gas or high magnetic field interruption. Don’t place the instrument in moisty or dusty area. Also, don’t place the instrument in area making it difficult to operate the disconnecting device.
    Openings on the instrument are designed for ventilation. To avoid overheating, don’t bloeck or cover the ventilating openings. For single machine operation, the ventilating openings on the instrument should be kept at least 20cm away from nearest objects. For simultaneous multiple machine operation, the distance between instruments should be no less than 10cm. additionally, don’t use the instrument on floppy surface; otherwise the ventilating openings at instrument bottom may be blocked.
    Over-temperature will affect the instrument performance or lead to faulty. Don’t use the instrument under direct sunlight and keep the instrument away from heater, furnace and other heat source.
    When the instrument is not in use for an extended period, unplug the power supply and cover the instrument with soft cloth or plastic paper to prevent dust entering in the machine.

14. Attention for operation During experiment operation, avoid liquid dripping on the instrument. When dealing with reagent, wear personal protective equipment such as protective gloves.

15. Re-transport If the instrument needs to be transported again, the detection hole position and the instrument should be thoroughly cleaned and sterilized with ultraviolet light before transportation.

16. Equipment Safety The instrument was designed, produced, and tested in accordance with EN 61010-1 (IEC 61010-1) “Safety requirements for electrical equipment for measurement, control, and laboratory use — Part 1: General requirements”. It has left the factory in a perfectly safe condition. The instrument meets the requirements of the Regulation (EU) 2017/746 of the European Parliament and of the Council of 5 April 2017 on in vitro diagnostic medical devices.

Attention: The instrument is Type-I equipment in line with GB4793.1 standard with protection level of IP20. The instrument is designed for indoor use.

• In any of the following cases, immediately unplug the power supply from the socket, and contact the supplier or ask qualified maintenance personnel for treatment:
• Liquid enters into the instrument.
• The instrument is exposed to rain or wet by water.
• The instrument fails to work normally, especially there is any abnormal sound or flavor.
• The instrument drops down or the shell is damaged.
• The instrument function has evident change.
• For any doubt concerning the compatibility between detergent and equipment part or materials contained in the equipment, please consult the manufacturer or its agent.
  Warning : The equipment should not be installed on the surface of inflammable materials!

Note: In case of the following situations, immediately unplug the power supply and seek assistance from the supplier or qualified maintenance personnel:

• Liquid enters into the instrument.
• The instrument is exposed to rain or water.
• The instrument fails to work normally.

modification to the information contained herein.

The document contains patent information under copyright protection. All rights reserved. Without prior written approval from Hangzhou Bioer Technology Co., Ltd., any part of the document shall not be copied, re-produced or translated into other language.

Before using the instrument for the first time, please carefully read the Operation Manual ！

Instrument label

A) Warning Sign

• Warning identification

GeneExplorer Thermal Cycler Instruction for Use

Warning mark

Warning! The instrument is pasted with a label of “HOT SURFACE!”. Any part of body is strictly prohibited from touching the metal part (module and hot lid) around the label during instrument operation or within a certain period after the program ends.

Other symbols on the packaging

Instrument calibration

Before the instrument is delivered, the device is fully calibrated according to the Bioer ‘s factory inspection standard, all the actions are under the requirements of ISO 13485 Medical devices – Quality management systems Requirements for regulatory purposes. If the instrument is installed in the required working environment, there is no need to calibrate or correct the temperature after installation.

Instrument maintenance

Periodically use clean, soft cloth dipped with ethanol to clean the block wells on module to ensure sufficient contact between test tubes and taper holes and to ensure favorable heat conduction. If there is any dirt on the instrument surface, use soft cloth dipped with cleansing cream to clean.

Warning! The power must be cut off when cleaning the instrument surface. Corrosive detergent is strictly prohibited from being used to clean the instrument surface.
The instrument module includes precise optics, dust, foreign matter and residue should be avoided.

Disposal Potentially infectious material and all parts that may come in contact with potentially infectious material must be disposed in accordance with the relevant legal provisions. All parts which have been replaced must be disposed in accordance with the relevant legal provisions.

• Disposal of the instrument must be carried out in accordance with the relevant legal provisions.
• Disposal of the packaging material must be carried out in accordance with the relevant legal provisions.
• After-sales service See the Warranty Card for specific content and scope of warranty.

Attention:

• After opening the packaging box, immediately check the objects contained therein based on the packing list. In case of any object damage or missing, please immediately contact the supplier.
• After acceptance inspection, fill out the Acceptance Inspection Form and send the copy (or fax) back to the company for filing and warranty service.
• After the packaging box is opened, please properly keep the packaging box and packing materials which will be used during maintenance. Hangzhou Bioer Technology Co., Ltd. will take no responsibility for instrument damage caused by improper packaging during transport to the maintenance department.

Introduction

The chapter covers introduction to PCR technology and examples of its applications, introduction to PCR Thermal Cycler and features of GeneExplorer Thermal Cycler.

Introduction to PCR technology
Polymerase Chain Reaction (PCR in short) is a nucleic acid amplification technology of in-vitro simulation of natural DNA reproduction process. With two DNA chains to be amplified as a template and mediated by a pair of artificially synthetic ODN primers, and through DNA polymerase of high temperature resistance, it can rapidly and specifically amplify specific DNA segment. Due to its simplicity, rapidity, peculiarity and sensitivity, ever since invention of PCR by Mullis in 1985 and discovery of DNA polymerase of high temperature resistance by Erlich in 1988, over a few years, the technology has been widely applied in various fields of bioscience. Especially in cytology, virology, oncology, genetic epidemiology, forensic pathology and flora and fauna immunology, it has accomplished outstanding achievements and become a milestone of contemporary molecular biology development.

Examples of PCR’s applications

Application of Thermal Cycler: The product is applicable to polymerase chain reaction in fields including immunology, human genome engineering, forensic pathology, oncology, organization  and population biology, paleontology, zoology and botany.

Intended use

• The GeneExplorer Thermal Cycler is an automated instrument used for qualitative amplify the related nucleic acids in human sample using the polymerase chain reaction process. The instrument is for in vitro diagnostic only.
• The GeneExplorer Thermal Cycler is intended for used in medical and biological laboratories by professional user trained in molecular biological techniques and the operation of GeneExplorer Thermal Cycler.

Features of GeneExplorer Thermal Cycler

GeneExplorer Thermal Cycler is a PCR of multiple functions and applications. It features as below:

• Providing up to eight replaceable modules based on requirements of various PCR experiments.
• Adopting advanced American ITI thermoelectric refrigeration technology and thermoelectric refrigeration element manufactured under Japanese quality management mode which presents stable and reliable performance;
• Maximum ramp rate up to 4C/s and can be adjusted based on experiment requirements.
• Multiple temperature control modes and higher reagent compatibility.
• Host machine of GeneExplorer Thermal Cycler can automatically recognize module and run corresponding program.
• Colorful large-screen display, humanized operation interface, simple and clear operation.
• Power-off protection function, and after power supply recovery, it can continue to complete the program unfinished.
• Hot lid can adjust height and pressure based on actual using condition to adapt to different reaction tubes and effectively prevent reagent evaporation and pollution.
• SB standard port can be connected to general computers, convenient for software upgrade.

Use of GeneExplorer Thermal Cycler

GeneExplorer Thermal Cycler, taking a leading position among similar products, has combined various advanced technologies and can meet requirements of various PCR experiments. Different modules have the suitable consumables, there are as follows.

• GE-96G / GE-96S:
  • (1) 1×96×0.2ml
  • 0.2ml standard centrifuge tube (2) 12×8×0.02ml
  • PCR 8-Strip Tube
  • (3) 96×1×0.2ml (96 wells PCR Microplate)
• GE-48D ： GE-48DG: GE-48DS:
  • (1) 48×0.2ml+48×0.2ml (48 wells PCR Microplate)
• GE-384G ： 384×0.02ml（384 wells PCR Microplate）
• GE-4I ： 4 pcs.of glass slide

Standard 0.2ml centrifuge tube

PCR Microplate

Glass slide

Service life of Thermal Cycler

Service life of Thermal Cycler is 5 years from the date of instrument installation.

Characteristics

The chapter mainly introduces the operation, transport and storage conditions of the instrument, and its basic parameters, performance and functions.

Normal operation conditions

• Ambient temperature: 10C ~ 30C RH: ≤70%
• Power supply: 220V～ 50Hz Altitude: ＜2km

Attention: Before using the instrument, please confirm if the working conditions can meet the above requirements. Pay special attention to reliable grounding of the power line.

Transport and storage condition

• Ambient temperature: -20C ~ +55C
• RH: ≤80%

Basic parameters

The parameters of the modules are as below:

Sample size

| ****

96×0.2ml

| ****

384×0. 02ml

| 48×0.2ml+4

8×0.2ml

| 44×gla ss slide| 48×0.2 ml+4

8×0.2ml

| 48×0.2ml+4

8×0.2ml

| ****

96×0.2ml

gradient temperature range| ****

30℃~99℃

| ****

/

| ****

30℃~99℃

| ****

/

gradient temperature difference

range

| ****

1℃~30℃

| ****

/

| ****

1℃~30℃

| ****

/

Ladder Temperature range| ****

/

| ****

0.1℃~6℃

Energy

consumption

| 600W| 1200W
Fuse| 250V 12A    5 20
LCD display| Color touch screen (8 inch, 260,000 colors)
Temperature range| 4.0-105°C (min. resolution: 0.1°C)
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File      saving amount| ≥2000 standard programs
Operation mode| Continuous operation
Dimensions| 420mm×269mm×254mm(L×W×H)
Net weight| 13kg
Attention:| When the set value of hot lid temperature is lower than the set value of module temperature, the hot lid temperature displayed on the screen will

be higher than the set value of hot lid temperature.

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Software functions

1. file edit (including file number, temperature and time of each section, setting of loop times, view, revise and delete file, automatic file connection, gradient temperature setting; maximum number of editable sections is 10, maximum number of steps is 100, maximum number of loop is 99, available for secondary nesting, gradient temperature range is 1℃~30℃; Ladder Temperature range 0.1℃~6℃，
2. file storage.
3. file modification (including adjustment of ramp rate, automatic temperature & time modification during cycling, time display and calibration).
4. setting of temperature, time and loop times during file running, and display of instant values (including voice prompt, estimation of overall and remaining time of file running, fault protection and warning);
5. stop file running (including pause file running, stop file running and automatic recovery after outage).

Attention:| The above software functions are only for reference. Please understand

that no further notice will be given for any modification to the software functions.

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Software version

Software version: V1.0

Preparations

The chapter mainly introduces the instrument structure, operation of touch screen, and preparations before turning on the instrument. If the instrument is used for the first time, please be familiar with the contents in the chapter before turning on the machine.

Structural schematic diagram
The structural parts of the instrument include heating module, hot lid, power switch module (including power switch, power socket, and fuse holder), and micro-computer controller and touch screen. All the modules of the instruments are the same except the heating modules, Diagram for GE-96G, GE-96S, GE-384G

 |  |  |
---|---|---|---
Attention: 1、 Re

 module

| quirements for fuse

‚ hot lid

| :  250V  12A     Æ5´20

ƒLCD screen

| . For fuse replacement,

„    pressure    balancing

… compression papdrod| u†ctpwohwicehr sioscikneltine wi| th‡theporewqeurirsewmitecnhts sh| ould be used. Contact the

supplier or manufacturer if you need fuse.

2、 compression pad for 96 wells PCR plate and 384 wells PCR.

3、 pressure balancing plate for 0.2ml single tubes and 8-strip tubes

Check before turning on the instrument

Before plug in and energize the instrument, please confirm the following:

1. Confirm the power supply meets the voltage required by the instrument; (see Chapter
   Two for power supply requirements)

2. Confirm that the power supply plug has been reliably inserted into the power socket;

3. The power line is reliably grounded.

Turn on the instrument
Turn on the power switch (switch to the position of “–”), and then the instrument will carry out self-test which will take about 20s, please wait patiently. If the self-test has discovered no problems, the main interface will show on the screen, and you can edit, view, revise or delete PCR amplification files.

Operation Instructions

In this chapter, the methods of editing, viewing, revising, and deleting PCR amplification files are introduced in detail, as well as the method of running PCR amplification files and setting of instrument system parameters.

Warning!| After the instrument is turned on, if there is any abnormal sound or display, or fault    alarm    and   prompt    present    during instrument    self-test,    please

immediately turn off the power and contact the supplier.

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Attention:| If the sample volume of your experiment is less than the number of cone holes of the module, please evenly distribute the test tubes to ensure stable press of the hot lid on the test tube tops, which will also contribute to uniform module loading and ensure uniformity of temperature change in each test

tube.

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Turn on the instrument

Turn on the power switch and then the instrument will carry out self-test, please wait patiently. If the self-test has discovered no problems, the main interface will show on the screen. Module Type is displayed at bottom left of the main interface. At the middle of the main interface, it displays four functions of software which are New Experiment, Open Experiment, Recently Opened and Settings.

Picture for GE-96G

Picture for GE-48DS

Picture of Model GE-384G/GE-4I/GE-96S would show the module type with 384G, plate and 96V respectively.

• Press the icon “New Experiment” to enter into the interface of experiment parameter setting.
• Press the icon “Open Experiment” to enter into the interface of file list.
• Press the icon “Recently Opened” to view experiment.
• Press the icon “Settings” to enter Personal Center and System Settings.
• During operation, instrument operation status is displayed on the main interface, and click to enter the interface of operation monitoring.
• When operation is stopped, click to enter the interface of experiment parameter setting.

PCR program editing
Click “New File” on the main interface or click “New Experiment” under “Open Experiment” or “Recently Opened” to enter into the interface of program setting.

Picture for “Open Experiment” or “Recently Opened” selection

Picture for “New Experiment” selection

The setting of program section
Select one section of the program and the section will light up; click “Section”, then there will prompt the checkbox of “Insert” and “Add”. Click “Insert”, a new section will be added before the section selected and lighting up. Click “Add”, a section will be added to the end of the program.
Click “Delete” to delete the section selected.

Section temperature and constant temperature time
On the interface of PCR program setting, click the small icon on upper right of the screen, and it will prompt the temperature and time setting box for setting temperature and constant temperature time of the program section.

• Run mode: Standard Mode and High-speed Mode.
• Standard Mode running characteristics simulate BIOER TOUCH series.

Function of loop
On the interface of PCR program setting, click “Loop”, and it will prompt interface 1-1, select the starting and ending sections of the loop, click “Determine” and it will prompt the setting box 1-2 for loop times, input the times and click “OK”, the setting of loop section will be finished.

Attention: In one program, multiple loop sections can be set, but these sections cannot be crossed, but only paralleled or included.

Interface 1-1

Interface 1-2

Extended parameter
On the interface of PCR program setting, click “Extended parameter” and it will prompt a box for setting extended temperature, extended time, speed, and gradient and other operations. Extended temperature: the volume of temperature increase or decrease for each loop. “-/+” changes the positive and negative of data.

• Extended time: time increase for each loop.
• Speed: ramp rate
• Gradient : gradients temperature refers to the temperature difference between the most left and most right ranks of the module. In “Temperature”, the temperature of the most left rank is set. GE-48DG, GE-96G, GE-384G own this function.
• Ladder Temp: It refers to the temperature difference between two adjacent modules. The range is 0.1℃~6℃. “Temperature” is set to the temperature of the leftmost module. GE-48DS, GE-96S own this function.

Save experiment
On the interface of PCR program setting, click “Save”, and input username and file name to save the file. Files saved can be found under “Open Experiment”.

Run program
Before running experiment, set up hot lid temperature, reaction volume, temperature control mode and control mode on the interface of PCR program setting and then click “Run” to start the experiment. You can also run experiment by directly selecting the file under “Open Experiment” and “Recently Opened”.

Click it will display the temperature of each module.

Temperature control mode

• Control Mode” represents the default temperature control mode after the system is turn on,and it includes two modes of “Block” and “Tube”.
• Block” mode only considers the temperature control of module during temperature ramp. As the temperature ramp of reagent is lagging that of module, the actual temperature change of reagent is inconsistent with our settings that the actual time of reagent reaching set temperature is shorter than the set time (see the figure below for the relation between module temperature and reagent temperature).

“Tube” mode considers the lagging reagent temperature ramp and provides an overshoot process after reaching set temperature to enable reagent to reach the set temperature more quickly. If setting time equal to that of “Block” mode, the actual time for reagent reaching set temperature is obviously extended (see the figure below for the relation between module temperature and reagent temperature).

File management
Click Open Experiment on the main interface to enter the interface of file management.
Click the dropdown list on the upper left to select disk for operation. For example, select USB flash disk to operate on USB flash disk contents/GeneExplorer.

• Press “New Experiment” to enter the interface of experiment parameter setting.
• Press “Run” to enter the interface of run.
• Press “Export” to export file or user.
• Press “Function” to delete and rename file or user.

Press “Function” to delete or rename user. Select user to export to USB flash disk. Click “Manage File”

Press “Function” to delete or rename file. Select file to export to USB flash disk.

System settings
Click “System Settings” on the main interface to enter the interface of setting which includes two major functions which are System Settings and Personal Center.

Personal Center

• In Personal Center, you can log on with registered account, or log out. 5.2 System Settings
• System Settings include the following 10 settings:
  • Network setting
  • Http remote access
  • Hot lid related
  • Time and date: set instrument time, and it will display on the lower right corner of the main interface
  • Instrument part use time
  • Dairy
  • About instruments
  • Tm calculation
  • Buzzer setting
  • Exit: exit system software

Software upgrade

UI program upgrade

Copy file to USB flash disk \version\update
Enter into System Settings->about instruments, select Firmware upgrade/

For setting menu, please pay attention to the capitalization of letters.

Driver upgrade

• File Copy to USB flash disk /GeneExplorer location
• Enter into System Settings->about instruments, select driver upgrade/

Fault Analysis and Troubleshooting

The chapter mainly introduces possible faults of the instrument, cause analysis and troubleshooting.

Fault analysis and troubleshooting

1

| ****

After the power switch is turned on, the display screen doesn’t work.

| Disconnecting power supply| Check and connect the power supply
Burned fuse| Replace fuse (250V 12A Ф5×20)
Damaged switch| Replace switch
Others| Contact      supplier       or manufacturer

2

| During file running, it shows “Err code: 0x**0000***,            and prompts “Please Contact the Manufacturer”| Module

contact

| sensor| damage| or| loose| ****

Contact      supplier       or manufacturer

Radiator sensor damage or loose contact
Hot lid sensor damage or loose contact

3

| ****

Evident change of module temperature rise rate or imprecise temperature control

| ****

Blocked ventilation holes

| Remove     the     blocking

objects     on     ventilation holes

Loose connecting line

| Open the shell and check the connecting line; tighten the screw of the terminal block if it is

loose.

Damaged TEC| Contact      supplier       or manufacturer

4

| Evident slowing module temperature fall rate or failure to fall below ambient temperature| Ambient temperature or humidity is

too     high,     and     exceeds     using condition of the instrument

| Please     properly     adjust

ambient temperature and humidity

Damaged TEC| Contact      supplier       or manufacturer
Damaged or un-working fan
5| Temperature changing| not| Damaged temperature sensor| Contact supplier       or manufacturer
Damaged TEC

6

| ****

The module fails to heat

| On      system      parameter      setting

interface, hot lid status is set as “OFF”.

| Set the hot lid status as

specific           temperature value

Temperature control time on file editing   interface   is   set as  “—- ”,

leading to automatic turning off of hot lid

| ****

Set temperature control time in digital form

Loose connector| Contact      supplier       or
 |  | Damaged heating component in hot lid| manufacturer
---|---|---|---
Damaged temperature sensor in hot lid
7| Abnormal         characters displayed on screen| Loose contact of PCB| Contact       supplier       or manufacturer
Damaged PCB

8

| ****

Reagent evaporation in reaction tube

| ****

Hot lid temperature is not set, hot lid is set as “OFF”.

| When setting parameters for running file,  select ON for hot lid control and set proper temperature
Uneven    distribution    of    reaction tubes| Adjust      reaction      tube distribution     to     ensure

symmetricity.

Untightened PCR tube caps

| Close      reaction      tubes

tightly and then put into the instrument

Explanation for Err code: 0x0000* is as below:

0X| Left sensor| Right sensor| Reserve d| Reserve d| Reserve d| Reserve d| Single               module hot

lid/double-modu le hot lid A

| Double-modul e hot lid B| Radiato r
---|---|---|---|---|---|---|---|---|---

0 normal 1 open circuit alarm 2 short circuit alarm/over-temperature radiator alarm

Attention:| 1.   During the warranty period, user is strictly prohibited from opening the shell of the instrument for inspection. In case of any fault requiring opening the shell for inspection, user should timely contact supplier or manufacturer.

2.   As temperature control features (including ramp rate, stability and volatility) differ for various brands and instruments, also due to the uncertainty of bio-experiments which can be easily affected by external environment, PCR program of successful running on one instrument may not achieve the equal effect on another. Therefore, when you are changing an instrument, you may need to adjust the

PCR running program for an ideal effect.

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Appendix 1  Wiring Diagram (the diagram is only for reference, and please understand  that no further notice will be given for any modification)

Appendix 2  Value and Meaning of Set Parameters

2

| ****

Time

| ****

Temperature control time (mm:ss)

| –:–

(press “+/-“ key before “:” to set)

| Infinite temperature control time and turn off hot lid in the meantime
00:00~59:59|

3

| ****

Ramp(C/S)

| ****

Temperature heating and cooling rate (C/s)

| GE-48DG,GE-48DS, GE-96S: 0.1-6.0

GE-96G,GE-48D:0.1-5. 0

GE-384G,GE-4I:0.1-4. 0

|

.#| Max. ramp rate

4

| ****

+Temp

| Temperature increase     for     each cycle (C)| ****

0.0~9.9

|
5| +Time| Time     increase    for each cycle (m:ss)| 0:00~9:59|

6

| Cycle1| Number of the cycle| 1~5| ****

Cycle1:×00 From 00 to 00

×00| Times of the cycle| 1~99
From 00| Starting    section    of the cycle| X(any existing section number)
to 00| Ending section of the cycle| ≤X+15

7

| ****

Grad.

| Gradient temperature (C)| ****

0 ~30

| For GE-48D, GE-4I, do

not          have          the parameters.

8| Date| Date (YY-MM-DD)| 00(~99)-01(~12)-01(~3

| YY-MM-DD
9| Time| Time (HH:MM:SS)| 00:00:00~23:59:59| HH:MM:SS
10| Default File| System default file name| Max. 11 characters|
11| Default User| System default user name| Max. 11 characters|

12

| ****

Control Mode

| ****

Temperature control mode

| ****

Block

| In this mode (enter into constant temperature status after reaching set temperature), the time for reagent at set temperature is less than

the set time (see Fig. 1).

 |  |  | ****

Tube

| In this mode (enter into constant temperature status when overshoot for about 3℃ after reaching                    set

temperature), reagent temperature can get close      to      the     set

temperature           more quickly (see Fig. 2).

---|---|---|---|---
13| Sample Volume| Sample volume (ul)| 10~200|
14| Holid| Hot lid off| Off|
Hot lid on (℃)| 30~110|
15| Key Sound| Key sound (yes/no)| Yes/No| Buzz
16| Run          End Sound| Run      end      sound (yes/no)| Yes/No| Intermittent buzz

Appendix 3 Product EMC Statement
Class-I Type-A Equipment
If the instrument is used under dry environment, especially under dry environment where artificial materials (artificial fabrics, carpet, etc.) present, electrostatic discharge may be generated which may lead to false conclusion.
The instrument is strictly prohibited from using near strong radiation source; otherwise, normal equipment operation may be interrupted.
EMC should be in line with the requirements of GB/T 18268.1-2010 and GB/T 18268.26-2010. 1. Requirements for immunity:

Table 1 Requirements for equipment immunity

Port| Test item| EMC       basic standard| Test value| Performance criterion
---|---|---|---|---

Shell

| Electrostatic discharge (ESD)|

GB/T 17626.2

| Air discharge:2kV,4kV,8kV; Contact discharge:2kV,4kV|

B

Radiation

magnetic field

| GB/T 17626.3| 3V/m,80MHz-2.0GHz,80%

AM

| A
Rated PFMF a| GB/T 17626.8| 3A/m,50Hz| A

AC

|

Voltage sag d

|

GB/T 17626.11

| 1 cycle 0%;| B
5 cycle 40%| C
25 cycle 70%| C
Voltage interruption d| GB/T 17626.11| 5%, duration:250 cycle| C
Pulse group| GB/T 17626.4| 1kV(5/50ns.5kHz)| B
Surge| GB/T 17626.5| Line to ground:2kV/ Line to ground:1kV| B
Radio

frequency transmission

|

GB/T 17626.6

| 3V,150kHz-80MHz,80%A M|

A

DCc

| Pulse group| GB/T 17626.4| 1kV(5/50ns.5kHz)| Not applicable
Surge| GB/T 17626.5| Line to ground:2kV/ Line to ground:1kV| Not applicable
Radio

frequency transmission

|

GB/T 17626.6

| 3V,150kHz-80MHz,80%A M| Not applicable

I/O signal b

| Pulse group| GB/T 17626.4| 0.5kV(5/50ns.5kHz)| Not applicable
Surge| GB/T 17626.5| /| Not applicable
Radio| GB/T 17626.6| 3V,150kHz-80MHz,80%A| Not
 | frequency transmission|  | M| applicable
---|---|---|---|---

I/O signal to main power supply

| Pulse group| GB/T 17626.4| 1kV(5/50ns.5kHz)| Not applicable
Surge| GB/T 17626.5| /| Not applicable
Radio frequency

transmission

|

GB/T 17626.6

| 3V,150kHz-80MHz,80%A M| Not applicable
A     The test is applicable only to equipment potentially sensitive to magnetism. CRT display interruption value is allowed to be higher than 1A/m.

B         Only applicable to cables longer than 3m.

C           Not applicable to input port expected to connect to battery or chargeable battery (before recharging, it should be removed or disconnected from the equipment). For equipment with DC power input port (using AC-DC power adapter), test should be conducted on AC input port of AC-DC power adapter specified by the manufacturer. If there is no specific requirement, typical AC-DC power adapter should be adopted. The test is applicable to DC power input port designed for extended long distance connection.

D “5/6cyle” refers to “5 cycles under 50Hz test” and “6 cycles under 60Hz test”.

|

Requirements for radiation:

Table 2 Requirements for equipment radiation

The equipment is designed and tested based on Type-A equipment in GB4824. In family environment, the equipment may cause radio interference, and thus protective measures should be taken.
Electromagnetic environment evaluation is suggested before the equipment use.

Appendix 4 List of Accessories

No.| Name| Model                  &

Specification

| Unit| QTY| Remark
---|---|---|---|---|---
1| Power line (with shield

magnetic ring clamp)

| 250V10A| String| 1| Accessory
2| Fuse| 250V12A, Φ5×20| Piece| 2| Accessory
3| WiFi Module|  | Piece| 1| Accessory
4| Ethernet Patch Cable|  | Piece| 1| Accessory
5| Compression Pad|  | Piece| 2| Accessory
6| Pressure         Balancing

Plate

|  | Piece| 1| Accessory

Hangzhou Bioer Technology Co., Ltd.

• Add: 1192 BinAn Rd, Binjiang District, 310053 Hangzhou,
• PEOPLE’S REPUBLIC OF CHINA
• Website: www.bioer.com.cn
• Tel:+86-571-85800535，87774558
• Fax:+86-571-85800537，87774559
• E-mail: instrument@bioer.com.cn

References

• 杭州博日科技股份有限公司
• 杭州博日科技股份有限公司

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