Life Technologies PS0300 PowerEase 300W Electrophoresis Power Supply User Guide

Life Technologies PS0300 PowerEase 300W Electrophoresis Power Supply

Specifications

• Catalog Numbers: PS0300, PS0301, PS0302
• Publication Number: MAN0008988 Revision D.0
• Intended Use: Research Use Only. Not for use in diagnostic procedures.

Product Description
The product is a power supply unit designed to meet most electrophoresis needs in a single, easy-to-use unit. It features a small footprint and stacking capability to save bench space, making it ideal for DNA/RNA electrophoresis applications.

Product Contents
The product includes the following components:

• Type G Power Cord – Quantity: 1 each
• Type CEE 7/7 Power Cord – Quantity: 1 each
• GB 15934-2008 Power Cord – Quantity: 1 each (PS0300)
• Type I Power Cord – Quantity: 1 each (PS0300), 1 each (PS0301, PS0302)

Regulatory Information
Ensure to use the correct power cord approved for your country/region. For example, North American customers should use Type B power cord, Japanese customers should use JIS 8303 power cord, Chinese customers should use GB 15934-2008 power cord, and Australia/New Zealand customers should use Type I power cord.

Operation Instructions

1. Upon Receiving: Examine the unit for any damage incurred during transit. File any damage claims with the carrier and keep the supplied box for inspection. Note that in-transit damage is not covered under warranty.
2. Features: The power supply offers Constant Voltage, Power, or Current modes and is capable of running multiple electrophoresis units simultaneously.

Repair and Maintenance
For repair and maintenance of the unit, refer to the documentation and support provided with the product. Regular maintenance is recommended to ensure optimal performance.

Documentation and Support
For detailed documentation and support, refer to Appendix F of the user manual or contact customer support for assistance.

FAQ

• Q: Can I use this product for diagnostic procedures?
  A: No, this product is intended for research use only and should not be used for diagnostic procedures.

• Q: What should I do if I notice damage to the unit upon receiving it?
  A: Examine the unit carefully for any damage incurred during transit. File any damage claims with the carrier and keep the supplied box for inspection. In-transit damage is not covered under warranty.

PowerEase® 300W Power Supply
Catalog Numbers PS0300, PS0301, PS0302
Publication Number MAN0008988
Revision D.0

Research Use Only. Not for use in diagnostic procedures.

Important Licensing Information:
These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
DISCLAIMER: LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED BY LAW, IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF.
©2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.

Introduction

Product contents
Contents

DEMKO, FIMKO, SEMKO, OVE, CE, ESC, IMQ
GB 15934-2008 Power Cord| 1 each (PS0301, PS0302)| CCC
Type I Power Cord| 1 each (PS0302)| NSW 18392
Quick Reference Card| 1 each|

Important: Some of the power cords appear similar; users should utilize the cord approved for their country/region. North America customers should use the Type B power cord and Japanese customers should use the JIS 8303 power cord. Chinese customers should use the GB 15934-2008 power cord and Australia/New Zealand customers should use the Type I power cord.

Upon receiving   the instrument
Examine the unit carefully for any damage incurred during transit. Any damage claims must be filed with the carrier, keep the supplied box for inspection. The warranty does not cover in-transit damage.
To ensure safe, reliable operation, always operate the PowerEase® 300W Power Supply in accordance with the manufacturer’s instructions. Always wear protective gloves and safety glasses when working in a laboratory environment. Avoid exposing the power supply to liquids as these may damage the unit and void the warranty.

Product description

Introduction
The PowerEase® 300W Power Supply is a microprocessor-controlled power supply designed to meet most electrophoresis needs in a single easy to use unit. The power supply’s small foot print and stacking feature conserve valuable bench space. This instrument is ideal for DNA/RNA electrophoresis, SDS-PAGE, and native PAGE. With four sets of output jacks that can be used simultaneously, the PowerEase® 300W Power Supply is designed to run applications at maximal power and efficiency. The PowerEase® 300W Power Supply offers three modes, Constant Voltage, Constant Current and Constant Power Mode. This manual describes the setup and operation of PowerEase® 300W Power Supply including important information on safety and maintenance of the unit.

Features of the PowerEase® 300W Power Supply

• Constant Voltage, Power or Current
• Capable of running multiple electrophoresis units
• LCD display with clear menu prompts for easy use
• Programming capabilities for limiting voltage (V), current (mA), power (W)
• Four sets of output terminals
• Capability to specify run durations

Description of buttons and switches

Operational keys
PowerEase® 300W Power Supply key pad

are flashing.
Ú (Down Arrow)| Toggle the cursor position down and decrease values when settings are flashing.
Mode| Select Constant Mode or Program Mode The Mode key cycles between voltage, current, power, and time settings, allowing

values to be changed.

Constant| Toggle between constant voltage, current, or power operation.
Start/Pause| Start, pause, and resume power supply operation without resetting the timer.
Stop| Stop power supply operation, reset the timer, turn off the beeper.

Methods

Getting started

Install the PowerEase® 300W Power Supply

1. Check the label located at the bottom of the power supply to ensure that the unit is compatible with locally provided voltage.
2. Place the PowerEase® 300W Power Supply on a level laboratory bench. Keep the area around the power supply clear to ensure proper ventilation of the unit.
3. For your safety: Position the unit properly such that the On-Off switch and the AC inlet located on the rear of the unit are easily accessible.
4. Ensure the AC power switch is in the Off position.
5. Attach the power cord to the AC inlet. Use only properly grounded AC outlets and power cords.
6. Connect the leads from the electrophoresis unit; insert the positive lead (+) into the positive (red) output jack, and the negative lead (–) into the negative (black) output jack.

Important guidelines

Introduction
The important guidelines for operating the PowerEase® 300W Power Supply are provided in this section. We recommend that you carefully review these guidelines before operating the instrument.
Important: For best results, do NOT use the PowerEase® 300W Power Supply at its maximum electrical load limits. Variations in buffer conditions can result in exceeding the power supply’s maximum voltage, current, or power output capacity and produce undesirable variations in performance.

Recommendation
The duration of electrophoresis can be defined in time (hours/minutes). When using this or any electrophoresis product, we recommend that you adhere to the protocols given in the electrophoresis product manuals, and durations, specified in time.
Important: For best results, follow these important guidelines when running multiple gels and electrophoresis units concurrently. For example:

• Avoid running samples of widely differing salt concentrations or sample buffers at the same time or on the same gel.
• Properly prepare your samples.

Note: Variations in conductivity due to differences in buffer salt concentrations can affect the run of all the samples run at the same time.
Note: For optimal performance, use Constant Voltage Mode when using the
PowerEase® 300W Power Supply with 1 – 2 protein gels or blots.

Operation modes

Introduction
The PowerEase® 300W Power Supply is designed to operate under different modes, Constant Mode or Program Mode, designed to meet most electrophoresis needs. Use the Constant Voltage, Constant Power or Constant Current Operation for applications that require only one specific voltage limit, current limit, or power limit continuously during the entire duration of electrophoresis.

Start-up
On power up use the arrow key to choose the operational Mode (Constant Mode or Program Mode), then press Mode to select.

On the Display Screen:

• After selecting Constant Mode, the unit defaults to the Constant Voltage mode and Volts (V) displays on the left side of the screen in large characters. The controlling value [Volts (V), Amps (A), or Watts (W)] is always displayed on the left-top side of the display screen in large characters.
• TIME is the first line on the right-top, and the other values (VOLT, AMP or WAT) are displayed in the second and third line on the right side of the display screen.

Constant operation protocol
Instructions for operating the PowerEase® 300W Power Supply in the Constant Mode are provided in this section. The Constant Voltage, Current, or Power Mode allows you to specify a voltage limit, current limit and power limit to be used during the entire duration of electrophoresis. Review the guidelines provided in this manual before starting electrophoresis using the PowerEase® 300W Power Supply.
A basic Constant Voltage, Current Mode, or Constant Power operating procedure of the PowerEase® 300W Power Supply is provided below. We recommend reading the guidelines provided in this manual for best results before starting an operation.
Note: The PowerEase® 300W is a high throughput power supply. For optimal performance, Life Technologies recommends using Constant Voltage when running 1–2 protein gels or blots.

1. Use the power switch on the rear side of the instrument to turn on the PowerEase® 300W Power Supply. On display screen Constant Mode and Program Mode will appear.
2. With the Constant Mode highlighted press the Mode key.
3. The Mode key toggles editing between VOLT, TIME (hours/minutes), AMP, and WAT
4. Press the Constant key to select the operation modes: Constant Voltage, Constant Current, or Constant Power from the display screen.
   • The screen displays the selected operation mode on the left side of the display.
5. Use the arrow keys to set the selected operation [voltage (VOLT), current (AMP), or Power (WAT)] to the appropriate values.
6. Use the Mode key to select TIME and use arrow key to set the time (hours/minutes) to specify the duration of the electrophoresis.
7. Press Start/Pause key to start electrophoresis. The Red LED illuminates when the electrophoresis is running.
8. Press the Start/Pause key again to temporarily interrupt power to ongoing electrophoresis without terminating the operation along with LED flashing.
9. Press Start/Pause key to restart electrophoresis.
10. Press Stop key to stop the electrophoresis.

Note: If the parameters need to be changed during the run press Start/Pause, enter the changes, and then press Start/Pause once again to restart the operation.
Note: After stopping and restarting an operation, the timer resets to the selected time.

Basic programming protocol
Instructions for operating the PowerEase® 300W Power Supply in the Program Mode are provided in this section. The Program Mode allows user to vary levels in voltage (V), current
(mA), and power (W) during specified periods of time as discrete changes (STEP) for up to 10 steps, depending upon electrophoresis needs.
A basic Program Mode operating procedure of the PowerEase® 300W Power Supply is provided below. For best result, read the guidelines provided in this manual before starting an operation.

Create a program

1. Select Program Mode by scrolling down using the ∨ key.
2. When highlighted press Mode key.
3. Select program file number using the arrow keys.
4. When program file number is located press the Mode key.
5. Select the number of steps by using the arrow arrow keys.
6. To enter the parameters of the run press the Mode key.
7. Voltage will appear on the display to enter voltage press Mode key.
8. To change voltage, use the arrow keys.
9. Select AMP or WAT by pressing the Mode key until desired parameter is flashing.
10. Increase or decrease the value by using the arrow keys.
11. To set the time in hours press the Mode key.
12. Increase or decrease the value by using the arrow keys.
13. Select the Mode key again to select minutes.
14. Increase or decrease the value by using the arrow keys.
15. Repeat 6 thru 14 above to create successive steps.

Edit a program

1. When a program file is selected the parameters can be edited by the Mode key.
2. The selected parameter will flash. Use the arrow keys to either increase or decrease values
3. Press the Mode key to migrate to the next parameter and then to the next step.

View a program

1. Select the program file number using the arrow keys, when highlighted press the Mode key
2. Press the Mode key to migrate to the next parameter and then to the next step.

Limiting parameter settings

Introduction
The PowerEase® 300W Power Supply is capable of operating at limiting Voltage, limiting Current, or limiting Power. We recommend operating the PowerEase® 300W Power Supply at limiting voltage for most applications. See below for more details.

Voltage limiting
For most electrophoresis methods resistance increases throughout the run. Limiting the voltage provides the following advantages:

• Current and power decrease throughout the run, providing an improving margin of safety over time.
• The same voltage setting can be used regardless of the number or thickness of gels being electrophoresed.

Current limiting
Discontinuous buffer systems and, to a lesser extent, continuous systems increase resistance during the run. With the current limiting setting on the PowerEase® 300W Power Supply, the voltage will increase as resistance increases to satisfy Ohm’s law (V=IR). If no voltage limit is set and a local fault condition occurs, such as a poor connection, very high local resistance may cause the voltage to increase to the maximum capacity of the power supply. This may lead to local overheating and damage to the electrophoresis cell or create unsafe conditions. When operating under constant current conditions, set a voltage limit on the power supply at or slightly above the maximum expected voltage.

Power limiting
Power is a function of voltage and current P=IV. For a typical gel run, the current will decrease causing the voltage to increase when run at constant power. The power limiting function may be used when running sequencing gels to remove the ammonium persulfate from the wells and to heat the gel to an optimal temperature for DNA separations.

Appendix A

General safety
WARNING! Protection impairment if used in a manner not specified by the manufacturer.

Instrument safety

Avoid electrical  shock
The PowerEase® 300W Power Supply produces up to 3A or 250 voltage outputs which are electrically isolated from ground to reduce the risk of electrical shock to the user. Follow the guidelines below to ensure safe operation of the unit.
The PowerEase® 300W Power Supply has been designed for use with electrophoresis cells with shielded banana plugs thus minimizing any potential shock hazard to the user. Life Technologies recommends against the use of unshielded banana plugs.

To avoid electrical shock:

1. NEVER connect or disconnect wire leads from the power jacks when the red indicator light at the Start/Stop key is on or when “RUNNING” is displayed on the screen.
2. WAIT at least 5 seconds after stopping a run before handling output leads or connected apparatus.
3. ALWAYS make sure that hands, work area, and instruments are clean and dry before making any connections or operating the power supply.
4. ALWAYS connect the power supply to a properly grounded AC outlet.

Avoid damage to  the instrument

1. For proper ventilation, leave at least 10 cm of space behind the instrument, and at least 5 cm of space on each side.
2. Do not operate the power supply in high humidity environments (> 95%), or where condensation may occur.
3. To avoid condensation after operating the power supply in a cold room, place the unit in a sealed plastic bag prior to removing the power supply from the cold room. After removing the power supply from the cold room, allow at least 2 hours for the unit to equilibrate to room temperature before removing the plastic bag and operating the unit.

Explanation of symbols and warnings

Symbols and  warnings

Product regulatory compliance

Federal  Communications Commission Advisory
This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at their expense. Changes or modifications not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.

Environmental design compliance
This equipment is compliant with Directive 2011/65/EU – European Union “RoHS Directive” – Restriction of hazardous substances in electrical and electronic equipment.

Appendix B

Troubleshooting

Observation| Possible cause| Recommended action|
---|---|---|---
The LCD screen| AC power cord is not connected| Check AC power cord connections at both ends.|
remains blank and the fan does not run when| | Use the correct cord.|
the power is turned on|
The fuse has blown| Replace the fuse|
Operation stops with alarm: The screen displays “ NO LOAD ”| Electrophoresis leads are not connected to the power supply or to the electrophoresis unit(s), or there is a broken circuit in the electrophoresis cell| Check the connections to the power supply and on your electrophoresis cell to make sure the connection is intact; check condition of wires in electrophoresis unit. Close the circuit by reconnecting the cables. Press Start/Pause to restart the run.|
High resistance due to tape left on a pre-cast gel, incorrect buffer concentration, or incorrect buffer volumes in the electrophoresis cell| Correct the condition by making sure the tape is removed from the pre-cast gel, buffers are prepared correctly, and the recommended volume of buffer is added to the electrophoresis unit.|
Operation stops with alarm: Display shows “ OVER VOLTAGE ”| Circuit is interrupted|

• Verify that the running buffer is correct.
• Verify the all cables are attached correctly
• Turn the Power switch off and on again; restart application.
• If you cannot restart the instrument, turn off the power, disconnect the power cord from the outlet, and contact Technical Service.

|
Operation stops with alarm: Display shows “ LEAKAGE ”| Ground leak detected during run| Check the electrophoresis system for improper grounding. Restart the power supply by turning the Power switch off and on.|
Operation stops with alarm:

Display shows “ OVER TEMP ”

| Power supply is overheating| Turn off power supply. Check for sufficient airflow around the power supply fan. After cooling down, restart the power supply by turning the Power switch to the on position.|
| |  If you cannot restart the instrument, turn off the power, disconnect the power cord from the outlet, and contact Technical Service.|

Common errors

• The electrophoresis system is not connected to the power leads, check the power leads
• The electrophoresis system has a short, the platinum wire is broken or the banana connectors are damaged
• Buffer concentration too low
• Buffer volume too low
• Short in power cord
• Current has dropped below acceptable rating (10 mA)

Short circuit|

• Load exceeds 3A
• Blown fuse in the power supply
• Incorrect input voltage (check input voltage switch near power inlet)

Change in load|

• Electrophoresis systems were added or removed during a run
• Buffer leaking in a connected system
• Excessive temperature increase
• Excessive buffer evaporation
• Loose connection in a connected system
• Current set too low

Change in constant mode|

• Voltage changes to current Current set too low. Ceiling hit and constant mode changed from voltage to current. Increase current to 3A.
• Current changes to voltage Voltage set to low. Ceiling hit and constant mode changed from current to voltage. Increase voltage to 250V

The PowerEase® 300W Power Supply has automatic cross over, set voltage or current, and preset power. During the electrophoresis process only one parameter is limiting at a time. The limiting parameter, together with the conductivity in the electrophoresis system, and the values for the other parameters determine the maximum output.

Appendix C

Operational electrical parameters

Ohm’s Law and Joule’s Law
Electrophoresis is the migration of a charged particle under the influence of an electrical field. The power supply output parameters voltage, current, and power are related by the following two equations:
Voltage (V) = Current (I) x Resistance (R); (V=IR)
Power (P) = Current (I) x Voltage (V); (P=IV)

Resistance
Resistance of the assembled electrophoresis cell is dependent on the conductivity of the gel buffer, the thickness of the gel, and the number of gels being run. Although the resistance is determined by the gel system, the resistance can vary over the course of an electrophoretic separation. For instance, in the Tris-Glycine buffer system, the fast moving, highly conductive chloride ions in the gel are gradually replaced by the slower moving, less conductive glycine ions from the running buffer as the gel runs. As a result, the resistance of the gel increases as the chloride/glycine front moves down the gel.

Voltage
The velocity with which an ion moves in an electric field will vary in proportion to the field strength (volts per unit distance). The higher the voltage the faster an ion will move.

Current
Current is a function of the number of ions passing a given cross-section of the circuit at a given time. For a given gel/buffer system, at a given temperature, current will vary in proportion to the field strength (voltage) and/or cross-sectional area (number and/or thickness of the gels). Ions in solution and at a given voltage will move faster as the temperature increases, increasing current.

Power
The power in Watts, or the rate of heat generated by the system, is directly proportional to voltage and current (P=IV).

Appendix D

Product specifications

230 VAC, 50/60 Hz

Fuses| One 4A/250V, one extra fuse is provided
Output power in watts| 300 watts
Output voltage range| 5~250 V
Output current range| 10 mA~3 A
Timer| ~99.99 per step
Terminal pairs| 4 (4 positive voltage and 4 negative voltage)
Operating Modes|
·        Constant Voltage| 1V step
·        Constant Current|
·        Constant Power| 10 mA step
| 1 watt step
Crossover| Automatic
Display type| Backlit LCD Graphic type
Display size| 53.64 x 15.64 mm (W x H)
Pause function| Yes
Safety features| No Load Detection Load Change Detection Overload Detection Ground Leak Detection Auto Restart
Programmable| Yes

• Store file no.: 20
• Program: up to 10 steps

Stackable| Yes
Housing material| Flame retardant ABS
Housing size| 200 x 290 x 70 mm (W x D x H)
Operating temperature| 0°C–40°C
Environmental condition| ≤95% RH (non-condensing), 75 KPa–106 Kpa, altitude not to exceed 2000 meters
Weight| 2.6 kg
Certifications| CE, cETLus, c-Tick
Warranty| 1 year

Appendix E

Repair and maintenance

Introduction
The PowerEase® 300W Power Supply requires no periodic maintenance program with the exception of an occasional dry wipe-down of the instrument.

Encountering  Problems
Check the troubleshooting section, see Appendix B
Contact Technical Support, see Appendix F
If the unit must be shipped back for repair, contact support for a Return Authorization Number and shipping instructions; see Appendix F

Replace the fuse
One extra fuse is supplies with the PowerEase® 300W Power Supply. For additional fuses, contact support; see Appendix F

To replace the fuse:

1. Turn off the main power switch at the rear of the PowerEase® 300W Power Supply and detach the power cord from the rear of the PowerEase® 300W Power Supply.

2. Open the fuse compartment located inside the power inlet by inserting a small flat blade screwdriver into the slot on top of the fuse compartment. Turn the screwdriver to gently pry open the fuse compartment.
   Note : The fuse compartment will not open with the power cord in place.

3. Pull the fuse holder out of the compartment and inspect the fuse. If the fuse is burned or there is a break in the fuse element, replace the fuse with an identical type of fuse (4A/250V) as provided in the fuse holder (see figure below).

4. Place the fuse holder back into the compartment.

5. Snap the cover closed.

Appendix F

Documentation and support

Services and support
For the latest services and support information for all locations, go to At the website, you can:

• Access worldwide telephone and fax numbers to contact Technical Support and Sales facilities
• Search through frequently asked questions (FAQs)
• Submit a question directly to Technical Support (techsupport@lifetech.com)
• Search for user documents, SDSs, vector maps and sequences, application notes, formulations, handbooks, certificates of analysis, citations, and other product support documents
• Obtain information about customer training
• Download software updates and patches

Safety Data  Sheets (SDS)
Safety Data Sheets (SDSs) are available at www.lifetechnologies.com/support

Certificate of Analysis
The Certificate of Analysis provides detailed quality control and product qualification information for each product. Certificates of Analysis are available on our website. Go to
www.lifetechnologies.com/support and search for the Certificate of Analysis by product lot number, which is printed on the box.

Limited product  warranty
Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies’ General Terms and Conditions of Sale found on Life Technologies’ website at www.lifetechnologies.com/termsandconditions. If you have any questions, please contact Life Technologies at www.lifetechnologies.com/support.

PowerEase® 300W Power Supply User Guide

References

• Help and Support | Thermo Fisher Scientific - US

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