nanoporetech PRO-INT002 PromethION 2 Integrated User Manual

nanoporetech PRO-INT002 PromethION 2 Integrated

USER MANUAL

Pre-installation

This Quick Start Guide contains everything you need to set up your PromethION™ 2 Integrated and to check that the device is ready for use.

Pre-installation

Before using the device, familiarise yourself with the following:

![This Quick Start Guide contains everything you

need to set up your PromethION™ 2 Integrated

and to check that the device is ready for use.

Pre-installation

Before using the device, familiarise yourself with the following:](https://manuals.plus/wp-content/uploads/2024/04/nanoporetech-PRO- INT002-PromethION-2-Integrated_1.png)

PromethION 2 Integrated user manual

community.nanoporetech.com/to/p2i

Safety and regulatory information

community.nanoporetech.com/to/safety

For detailed information and troubleshooting, view the user manual.

What’s in the box

• The PromethION 2 Integrated ships with 5 x power cables (1 US, 1 UK, 1 EU, 1 CN, 1 AUS) for international use.

1. Set up your device

1. Unpack your PromethION 2 Integrated device*
2. Attach the cables and peripherals in the order shown opposite
3. Press the power switch

Rear input/output

• The device must be placed away from direct sources of heat or sunlight. Place device on a well-supported, strong, clean bench. Allow 30 cm clearance rear and sides, and do not cover ventilation grilles. See user manual for detailed installation advice.

• Connect only one monitor

2. Log in to your PromethION 2 Integrated

On initial setup, we recommend using an external monitor. For subsequent uses, you can use the touchscreen and/or an external monitor.

Monitor:

1. Log in to your PromethION

• Username: prom
• Password*: prom

2. Open MinKNOW™

• Click the wheel icon on the desktop to load MinKNOW.

3. Log into MinKNOW.

• Use your Oxford Nanopore account details.

• For added security, we recommend changing your password after device setup using the instructions in the user manual.

3. Update MinKNOW

For the latest sequencing features, update MinKNOW:

You must now shut down your PromethION 2 Integrated using the instructions in step 4. Once complete, press the power switch and repeat step 2.

4. Power off

Ensure the device is not performing any tasks and remove any CTCs  from the flow cell positions if present.

In MinKNOW, click or press (if using the touchscreen) the top-right power icon, then Shutdown in the pop-up window.

After repeating step 2, you will be able to complete your software update.

Then, proceed to step 5.

5. Perform hardware check

A hardware check is required before performing your first PromethION 2 Integrated sequencing run. To run a hardware check, follow the on-screen instructions in MinKNOW, then follow the instructions below. You will require your two PromethION Configuration Test Cells (CTCs).

Hardware check overview:

1. Insert the CTCs into the device as shown here and close the lid of the device.
2. In the MinKNOW software, the flow cell status indicators (the two boxes) will change colour from grey to white.
3. Press Select all available. This will change the colour of the flow cell status indicators (the two boxes) on the MinKNOW Hardware check panel to dark blue.
4. Press Start in the bottom right.
5. Check the flow cell positions show to pass the hardware check.
6. Remove the CTCs from the flow cell positions after you complete the hardware check.

Note: If your hardware check fails, see Support in the Additional information section.

6. Discover the Nanopore Community

community.nanoporetech.com

Ensure the success of your nanopore sequencing project and stay up-to-date with the latest technology and protocols.

Tip: Learn how to analyse your nanopore data:
nanoporetech.com/analyse

Additional information

Warranty

A license and warranty can be purchased for your device here:
store.nanoporetech.com/device-warranty.html
Flow cell warranty: community.nanoporetech.com/to/warranty

Recycle used flow cells
Oxford Nanopore is committed to environmental sustainability.
You can help by sending your flow cells for recycling.
Find out how: community.nanoporetech.com/support/returns

Place your next order
Buy more consumables at the Oxford Nanopore Store:
store.nanoporetech.com

Documentation
Documentation for your device is available on the Nanopore Community: community.nanoporetech.com/docs

Support
For all of your customer and technical support needs, visit: community.nanoporetech.com/support

Technical specification

PromethION 2 Integrated

4 x USB 3.0 type-A ports 1 x HDMI port
1 x DisplayPort
Software installed| Ubuntu
MinKNOW, EPI2ME
Compute specification| 5.5” touchscreen 15 TB SSD storage 64 GB memory| 1 x Intel Core i7 (12-core)
1 x NVIDIA Ampere series GPU
Environmental conditions| Functional range of electronics is within environmental temperatures of +5°C to +40°C. Users should allow 30 cm clearance to the rear and sides of the device.
Designed to sequence in environmental temperatures of +18°C to +25°C. Use within 30%–75% relative non-condensing humidity limits.
Intended for indoor use.
Can be used up to altitudes of 2,000 m. The device has a Pollution Degree 2.

Specifications:

• Product: PromethION 2 Integrated

• Manufacturer: Oxford Nanopore Technologies

• Includes: Power cables (US, UK, EU, CN, AUS), Test Cells
  (CTCs)

• Compatibility: DisplayPort/HDMI monitor, Ethernet cable, USB keyboard, USB mouse

Oxford Nanopore Technologies
phone +44 (0)845 034 7900
email support@nanoporetech.com
What’s in the box @nanopore

www.nanoporetech.com

Oxford Nanopore Technologies, the Wheel icon, MinKNOW and PromethION are registered trademarks of Oxford Nanopore Technologies plc in various countries. All other brands and names contained are the property of their respective owners. © 2024 Oxford Nanopore Technologies plc.
All rights reserved. Oxford Nanopore Technologies products are not intended for use for health assessment or to diagnose, treat, mitigate, cure, or prevent any disease or condition.
ONT-08-01121-00-1 | BR_1221(EN)_V1_01Jan2024

FAQ:

Q: What should I do if my device encounters an error during setup?

A: If you encounter errors during setup, refer to the user manual for troubleshooting steps. You can also contact Oxford Nanopore Technologies for support.

References

• Oxford Nanopore Support | Oxford Nanopore Technologies
• Oxford Nanopore Support | Oxford Nanopore Technologies
• Community
• nanoporetech-customers - Sign In
• Community - Flow Cell Check - Introduction to the flow cell check
• Oxford Nanopore technologies data analysis solutions
• store.nanoporetech.com
• store.nanoporetech.com/device-warranty.html
• Welcome to Oxford Nanopore Technologies

Read User Manual Online (PDF format)

Read User Manual Online (PDF format)  >>

Download This Manual (PDF format)

Download this manual  >>