VIA-CELL Bioaerosol Sampling Cassette User Manual
- June 13, 2024
- VIA-CELL
Table of Contents
Bioaerosol Sampling Cassette
Bioaerosol Sampling Cassette
For the collection of viable and non-viable airborne
particles
Product Information
-
Product Name: Bioaerosol Sampling Cassette
-
Model Number: LA03023 Rev 8
-
Designed for the collection of both viable and non-viable
bioaerosols -
Uses a proprietary water soluble collection media
-
Maintains the viability of spores during transport to the
laboratory -
Allows analysis of particles using one or more analytical
methods
Applications
-
Culturable Sampling: Collects viable organisms for culturing on
agar media plates -
PCR Analysis: Ideal for PCR and other chemical types of
analysis -
Direct Microscopy: Allows pre-screening of samples using direct
microscopy methods
Principles of Operation
The Via-Cell sampler uses a unique air flow path to collect
particles.
Operational Performance
The graph above shows the high collection efficiency of the
Via-Cell sampler at a flow rate of 15LPM.
Recommended Field Sampling Procedures
The following procedures are recommended for efficient and safe
use of the Via-Cell sampler:
Required Sampling Equipment:
-
Via-Cell Sampling Cassettes
-
Sampling pump capable of pulling 15LPM
-
Tubing to attach cassette to pump, if required (1/4 ID or 1/2
ID) -
Pump calibration device
Handling the Via-Cell Sampler:
The Via-Cell Sampler should be handled carefully to avoid
contamination:
-
Store Via-Cell cassettes in climate controlled areas with a
room temperature between 50 – 80 degrees F -
Avoid careless handling or insertion of anything into the
cassette through the inlet or outlet port to prevent
contamination
Bioaerosol Sampling Cassette
For the collection of viable and non-viable airborne particles
Laboratory & User Manual
LA03023 Rev 8
INTRODUCTION
The Via-Cell sampler is a unique, multi-purpose particle collection device
designed for the collection of both viable and non-viable bioaerosols. Using a
proprietary water soluble collection media along with special environmental
controls, the Via-Cell sampler is capable of collecting particles, while
maintaining the viability of spores during transport to the laboratory. The
particles can then be analyzed using one or more analytical methods.
· Indoor Air Quality · Allergy Testing · Flood Restoration
APPLICATIONS
· Clean Room Monitoring · Hospital Infection Control
VIA-CELL ADVANTAGES
· Preloaded, sterile and ready for use.
· Disposable, no equipment to clean between samples.
· Eliminates cross contamination concerns.
· Includes tamperproof safety seal to prevent sample contamination after
collection.
· Each slide inside the sampler is marked with a unique serial number to
ensure accurate sample traceability.
· Shelf life of 1 year from date of manufacture is 4 times longer than most
sampling methods using culture plates.
· Allows multiple types of analysis to be performed from a single collected
sample.
· Samples do not require refrigeration, only room temperature storage and
transportation.
· Small, compact size. No heavy or bulky equipment is required.
· Can be used with virtually any kind of sampling pump capable of pulling a 15
lpm (vacuum) air flow, including battery powered pumps such as the Zefon Bio-
Pump® or Bio-Pump® Plus.
2
ANALYSIS OPTIONS
Culturable Sampling:
The Via-Cell sampler is the perfect tool for collecting viable organisms and culturing them on agar media plates.
PCR Analysis:
The Via-Cell utilizes a water soluble collection media which is ideal for PCR and other chemical types of analysis.
Direct Microscopy:
Samples collected using the Via-Cell sampler may be “pre screened” using direct microscopy methods which allows the analyst to decide if additional analysis techniques are needed.
Via-Cell sample
Make decision on analysis type
Culture the sample
Direct microscopy screening
Is further analysis necessary?
Yes
Culture the sample on agar media
Process using PCR or other chemical means
Process using standard direct
microscopy
3
PRINCIPLES OF OPERATION
The Via-Cell sampler operates on the well established principle of inertial
impaction. Particles in the air stream are accelerated as they approach the
tapered inlet opening and drawn through a small slit aimed directly at a glass
slide. This glass slide contains a water soluble sampling media which is
designed to hold particles intact and maintain viability for later analysis.
As the particles come through the slit, the air velocity forces the particles
to impact into the sampling media, while the air stream makes a sharp 90o turn
and proceeds around the slide and out of the cassette. The air flow path
through the Via-Cell sampling cassette is illustrated below in Figure 1.
Figure 1
AIR FLOW PATH OF THE VIA-CELL SAMPLER
4
OPERATIONAL PERFORMANCE
The Via-Cell sampler has been evaluated in commercial and university
laboratories to determine the collection efficiency and operational aspects of
the collector. With impactor-type samplers, the most common measure of
efficiency is defined by it’s “cut-point” curve. The cut-point (also referred
to as the “D-50” point) is defined by the particle size at which 50% of the
particles are collected and 50% pass through the sampler at a pre-determined
flow rate. The `cut point’ for the Via-Cell Sampler has been determined to be
1.56 microns at a flow rate of 15 LPM. This cut point is ideal for fungal
analysis because particles smaller than this are not of interest, and may even
create enough background debris to obscure adequate viewing of the sample and
compromising reliable analysis.
Figure 2
Collection Efficiency, %
100 90 80 70 60 50 40 30 20 10 0 1
Zefon “1.5” µm Cutpoint Impactor Calibration
1.664µm at 97.8% 1.604µm at 77.1%
W = 0.077 cm S/W = 0.50 Re = 2,310 Dp = 1.56 µm
50
GSD = 1.05
1.553µm at 45.1% 1.508µm at 26.4%
1.398µm at 7.1%
10
Aerodynamic Particle Diameter, µm
The graph above displays the collection efficiency of the Via-Cell sampler
when used at a flow rate of 15LPM. Samplers that are designed properly will
exhibit upper and lower curve limits that are very sharp and in an ideal
situation, a sampler will reach 100% efficiency almost immediately after the
cut-point. The Via-Cell demonstrates a remarkably sharp curve that is superior
to many other devices on the market today.
5
RECOMMENDED FIELD SAMPLING PROCEDURES
The Via-Cell sampler is designed to efficiently collect bioaerosol particles
while also providing safe transport to the laboratory. The results achieved
when using the sampler requires proper use, including pump calibration and
handling techniques. Therefore familiarization with the Via-Cell Sampler
collection procedure is recommended prior to use.
Required Sampling Equipment
- Via-Cell Sampling Cassettes Sampling pump capable of pulling 15LPM Tubing to attach cassette to pump, if required (1/4″ ID or 1/2″ ID) * Pump calibration device
Handling the Via-Cell Sampler:
The Via-Cell Sampler is sterile and ready-to-use when received. It is very important not to contaminate the inner-surface of the cassette through careless handling, or insertion of anything into the cassette through the inlet or outlet port. While the collection may render the sampler not sterile due to the collected particles, the person doing the collection should not introduce spurious materials into the collection that could alter the outcome.
Handling Tips and Considerations
1. Always store Via-Cell cassettes in climate controlled areas with a room temperature between 50 – 80 degrees F.
2. The Via-Cell sampler does not require refrigeration during storage or during shipment to the analysis laboratory.
3. Prolonged exposure to high temperatures (e.g. as that in the trunk of a car or hot warehouse) may alter the performance of the product.
4. Use of the Via-Cell sampler in extreme environmental conditions (i.e. very hot or cold weather) may affect the collection performance.
5. Always ship Via-Cell samples to the laboratory so that the laboratory receives the samples within 24 hours after collection. The laboratory should process the samples within 24 hours of receipt.
6. Zefon recommends wearing gloves when handling the Via-Cell cassette.
6
EXPLODED VIEW OF VIA-CELL CASSETTE
Figure 3
Blue Inlet Cap Controls environmental conditions during storage and transport.
Remove immediately prior to sampling and replace immediately after sampling.
Main Body (clear plastic section) This section holds the collection media and
is the section used during sampling. Note: Plastic may have an amber tint.
This is considered normal and caused by the radiation used during
sterilization.
Cassette Outlet Connect pump tubing here.
Blue Outlet Plug Remove during sampling and replace immediately after
sampling.
WARNING!
Do not open the blue inlet cap assembly! The substances used inside may be
irritating to your skin.
7
SAMPLE PREPARATION – CALIBRATION
The Via-Cell sampler requires the sampling pump to be calibrated at a flow rate of 15 liters per minute to ensure proper collection. Ideally, calibration should be checked prior to and after each collection and as a minimum once per day. It is strongly recommended that a primary calibrator certified to a NIST standard be used to calibrate the sampling pump. Secondary calibration devices such as a rotameter may be used if it is calibrated against a primary standard.
Below are examples of typical devices that can be used for calibration:
Zefon Bio-Pump®
Primary Calibrators
Secondary Calibrators
TSI 4046 Primary Flow Calibrator
Zefon Via-Cell /Air-O-Cell CSI Flowmeter (#ZBP-305)
High Volume Pumps
(includes AC powered rotary vane, diaphragm and linear pumps)
Primary Calibrators
Secondary Calibrators
Gilian Gilibrator Electronic Bubble Meter
Rotameter flow meters
TSI 4046 Primary Flow Calibrator
Bios DryCal® Electronic Calibrator
Please note that some pumps, including the battery powered Zefon Bio-Pump®, will only work with specific calibration devices. Please reference the operation manual for your pump to determine if special calibration devices are needed.
8
Typical Bio-Pump® Calibration Setup
Via-Cell®/Air-O-Cell® CSI Flowmeter
Bio-Pump® Plus with TSI Calibrator
The Via-Cell flowmeter used with the Zefon Bio-Pump® has the in-line resistance of a sampler built into the calibration scale. When using the TSI 4046 Primary Flow Calibrator, actual samplers are used in-line during calibration. For complete calibration instructions, please refer to the instructions included with your pump or calibration device.
Typical High Volume Pump Calibration Setup
Direct Connection to Electronic Calibrator
In-Line Connection to Electronic Calibrator
The Via-Cell sampling cassette does not produce significantly measurable backpressure and does not impede the performance of most high volume rotary vane, diaphragm or linear pumps. Therefore the calibration device may optionally be connected directly to the pump (without a Via-Cell sampling cassette in line) to calibrate the pump flow rate. When choosing to calibrate with a sampler in-line, it is recommended that one sampler is retained as a permanent calibration cassette and used for this purpose each time.
9
COLLECTING A SAMPLE
1. Calibrate the sampling pump to a flow rate of 15 lpm. 2. Clean hands or
wear clean, disposable, powder free gloves. 3. Tear open the foil package
using the tear strip on top. Use care when opening
as this package is re-sealable and is required to be used after sampling for
transport to the laboratory. 4. Remove the Via-Cell from the package. 5.
Remove the blue inlet cap and record the Via-Cell serial number, along with
any other pertinent information on the front of the foil package. The serial
number can be viewed through the top of the cassette once the blue inlet cap
is removed. It is printed directly on the collection slide as shown in Figure
4. After recording the serial number, replace the blue inlet cap until ready
to sample. 6. Remove the blue outlet plug from the cassette and place the plug
into the foil pouch for safe keeping.
Figure 4
10
ABOUT THE FOIL PACKAGING
It is important to retain the resealable foil package for transportation of
the cassette to the laboratory. This package along with the blue inlet and
outlet caps combine to control the environmental conditions inside the
cassette after sample collection. This is critical in maintaining the
viability of the particles if culture analysis is required. The included
safety seal label is used to ensure your collected sample remains
uncontaminated until it reaches the laboratory.
CAUTION
The interior of the Via-Cell sampler is sterile. Care needs to be taken when
handling the product with the plugs removed so that the sampling port and
outlet port do not become contaminated with materials that may alter the
collection or analysis of the sample (e.g. introduce materials not normally
collected in the air stream).
7. Connect the Via-Cell sampler to the pump and position the cassette in the desired location. The Via-Cell sampler is capable of operating in any vertical or horizontal orientation and in confined spaces such as ducts, plenums or wall cavities.
8. Remove the large blue inlet cap from the cassette and place into the foil package for safekeeping.
9. Turn on the pump and run for the desired amount of time. Reference the chart on page 12 for assistance in determining the correct sampling time.
10. When the sampling period is completed replace the blue plug in the outlet and the blue cap over the inlet. Handle these items by the outer finger grips. The plugs will prevent contamination of the interior of the cassette during transport, and ensure proper environmental conditions of the collected sample.
11. Place the Via-Cell sampler into the
special foil bag and zip it closed. Apply
the red safety seal label over the top of the
foil bag opening. This will ensure the integrity
of your sample until it reaches the laboratory.
Note the sample time and other important
sampling information. Send the cassette to
the analysis laboratory to arrive within
24 hours after the sample was collected.
Refrigeration of the collected sample is not
L0304210 Rev 2
required during transport.
11
SAMPLING TIME RECOMMENDATIONS
TheVia-Cell sampler collection period is dependent upon the density of the
particulate in the environment. When sampling in a dusty environment, the
particles can overlay each other on the slide causing difficulties in direct
microscopy analysis. In addition particles will not adhere to the adhesive
media if the adhesive is already covered by particles.
The following chart suggests typical sampling times to attain a particle
deposition on the slide in which the edges of the trace are sharply defined
and the particles dispersed properly to enable good microscopic evaluation.
Longer/shorter collection times may be required. Each situation must be
evaluated according to specific conditions and the Via-Cell sampler sampling
time must be adjusted to meet those conditions.
RECOMMENDED SAMPLING TIMES
Environmental Dust Conditions · Clean office environment or outdoors (no
visible dust)
· Indoor environment, high activity
· Indoor environment, evidence of drywall renovation or industrial dust
Sampling Time 10 minutes 5 minutes 1 minute
CONTROL SAMPLE RECOMMENDATIONS
Using one or more of the following control sample recommendations is
considered a good sampling practice that will help provide some basic
guidelines to interpreting results:
1. Collection of an outdoor sample may help to determine the amplification of
indoor particles.
2. Collection of a sample in a `non-suspected’ area may assist with the
comparison of results. This can also sometimes be used in place of an outdoor
sample when conditions outside are not favorable for sampling, such as on a
rainy day.
3. Field blanks are a recommended sampling procedure.
12
LABORATORY ANALYSIS STRATEGIES
The Via-Cell sampler is unique in that the particulate collected may be
analyzed using one or more analytical methods such as culturing, microscopy,
PCR (polymerase chain reaction), or other types of analysis. Because the media
on the Via-Cell slide is water soluble, and the lower half of the sampling
cassette has a built-in trough, the sample collection may be suspended in
water directly in the cassette without additional glassware. This suspension
can then be transferred and used in any number of analytical methods that can
use this suspension.
NOTE
Aseptic (sterile) handling procedures must be employed by laboratory personnel
to prevent the introduction contamination that could affect analysis.
The collected sample must be processed in the laboratory in a timely manner.
Experimentally it has been shown that collected mold particles (for example,
Cladosporium cladosporioide) can maintain viability inside the Via-Cell up to
5 days after collection. However, the best viability and resulting growth was
achieved within 3 days. After 3 days, some diminished viability will occur.
For this reason it is recommended that the collected sample reach the
laboratory within 24 hours after collection and that the sample should be
processed by the laboratory within 24 hours after receipt. Processing may
include screening, creation of the suspension and culture. If analysis as PCR
is done, the collection can remain on the collection slide much longer as
viability of the particles is not a factor.
Please note that if culturing of the collection is anticipated, staining of
the collection will not be possible for prescreening the particles with direct
microscopy since stain kills the viable particles. If PCR analysis is
anticipated, staining may be possible; it will be up to the user to determine
the suitability of the stain and possible interference with the analysis.
NOTICE
Each Via-Cell sampler should have a plug installed into the outlet port of the
cassette as well as the cap over the inlet port when received by the
laboratory. These are necessary to seal the cassette to control the
environmental conditions as well as prevent contamination during shipment. The
outlet plug will seal the bottom of the cassette so water can be added to
dissolve the media and suspend the particles. If the user did not re-install
the plugs after collection, the ability of the mold particles to be grown may
be compromised.
13
SAMPLE PREPARATION FOR CULTURING OR PCR
The Via-Cell sampler can be prepared for culturing, PCR and other analysis by
suspending the particles in sterile water, then dispensing the suspended
particles onto agar plates or other media.
Required Supplies
-Syringe – 3cc sterile, or sterile pipettes -Sterile water -Culture plates with appropriate agar -Sterile particle spreaders -Forceps (self-closing type recommended) -Controlled environmental chamber
1. Verify that the plug and cap are installed in the cassette. Record the
sampler serial number onto the lab worksheet. The serial number is printed
directly on the slide, not on the outside of the cassette.
2. Clean the outer surface of the sampler to remove any excess dirt. Cut the
protective tape. Open the cassette in the controlled environmental chamber,
keeping the slide in position as shown below. Be careful not to get pieces of
tape inside the sterile cassette.
3. The collection slide will be facing media-side down toward the V shaped
inlet sampling port. When you can read the word “ZEFON” on the collection
slide, the media will be facing you.
Collection Slide Media side faces the slit opening (the media is facing down
in this picture)
Trough in bottom half of cassette
14
4. Put 2cc sterile water into the trough in the bottom half of the cassette.
After cleaning the tweezers tip with alcohol, place the slide upside down
(media side down) on top of the water. If any large bubbles are trapped under
the slide, gently lift one side of the slide and slowly lower to remove
bubbles. NOTE: Be sure
the alcohol has completely dried before handling the slide.
5. Leave the slide exposed to water for a minimum of 5 minutes
6. After 5 minutes, raise the slide with the forceps and flush the face of
the slide with 1/4 cc of sterile water. Allow water to fall into cassette. If
the adhesive does not completely wash off the slide, let the slide sit an
additional 5 minutes to dissolve the remaining adhesive.
7. Draw the suspension from the cassette housing into a sterile syringe or
pipette, then dispense onto a culture plate.
Culture Plating Tip
It may be desirable to split the suspension onto several culture plates.
Particles such as molds and funguses germinate in less than 100% RH (liquid
water). Using more water to dissolve the media may result in a longer time for
the water to evaporate from the surface of a culture plate and growth to
occur.
15
DIRECT MICROSCOPY PROCEDURES
General
This procedure will enable the analyst to overview the particles on the slide
and then determine the next course of action to take (e.g. culture the
sample). If culturing the sample after microscopic analysis is a possibility,
it is important to aseptically handle the slide with sterile instruments to
maintain sterility of both the slide and the inside of the cassette. Analysis
of the collected sample should be performed by those experienced in particle
identification.
Required Supplies
-Standard 1 x 3 microscope slide -Microscope -Stain or linking solution (see stain notice below) -Forceps (self-closing type recommended) -Controlled environmental chamber -Zefon Via-Cell Sterile Microscopy Linking Solution -Cover Glass
Sample Preparation
1. Prepare a standard microscope slide by sterilizing’ with alcohol. 2. Record the Via-Cell sampler serial number onto the lab worksheet. 3. Cut the adhesive label around the Via-Cell cassette. 4. Aseptically open the cassette in the environmental chamber taking care not to touch the inner surfaces of the cassette. 5. Remove the slide using forceps (tipssterilized’ with alcohol) and place onto
the sterilized microscope slide with the media side up (when the media is
facing up, you can read the printing on the slide). DO NOT place the Via-Cell
slide face down onto the microscope slide as transfer of the adhesive and/or
particles may occur. 6. Place the slide under the microscope for examination.
Be careful not to contaminate the slide.
NOTICE
Stains such as LactoPhenol Cotton Blue or Saffron Red contain solvents that
will destroy the ability for spores to grow. DO NOT use any type of stain if
you plan to culture the sample after examination
16
DIRECT MICROSCOPY SLIDE PREPARATION
For use when standard direct microscopy is required and the sample may later
be cultured.
A NOTE ABOUT LINKING SOLUTION
The use and application of linking solution is an important step when
examining Via-Cell slides under direct microscopy and/or when viability of
particles needs to be preserved. The adhesive on the Via-Cell slide has a
frosty appearance in its natural form. This needs to be cleared before use in
direct microscopy. Use of linking solution solves this. The linking solution
works by partially dissolving the adhesive. Therefore it is necessary to place
enough solution onto the slide that will allow the cover slip to carry the
solution completely under the cover slip. Otherwise blotchy, non-clarified
areas may be seen under the cover slip.
Step 1 Place the Via-Cell slide onto the microscope slide media side up.
IMPORTANT: Do not permanently mount sample to microscope slide. This preserves
the ability to culture the sample.
Via-Cell® slide with media facing up
Sterile Microscope Slide
IMPORTANT!
Application of the linking solution must be done very carefully and in a
specific manner to avoid disturbing the collected sample trace. Please follow
the directions regarding proper application of linking solution precisely.
17
Step 2 Place a generous amount of linking solution along the edge of the
slide. DO NOT place drops of linking solution directly on the trace area. This
will result in excessive disturbance and shifting of particles.
Step 3 Lower the cover slip on top of the slide gently at an angle as shown.
Placing the cover slip on the slide this way allows the linking solution to
spread across the slide while disturbing the trace as little as possible.
18
Preparation is now complete Slides take several minutes to clear. Here is an
example of a before and after look at the slide. Your slide should exhibit
clarity similar to the top example.
Direct Microscopy Preparation Application Notes:
1. Always sterilize a cover glass to place over the collection if you intend
to later culture the sample.
2. Sterilization is not necessary if you do not intend to culture the sample.
3. Use of any type of stain or solvent should only be used if you do not
intend
to culture the sample. Some stains may not clear the Via-Cell slide
adequately. If this happens, contact Zefon and we can provide guidance or sell
a stain that will clear adequately. 4. If using immersion oil, apply it
carefully. If immersion oil makes contact with the Via-Cell adhesive, it will
probably result in killing any viable particles. 5. Always rinse the cover
glass with about 1/4cc water into the cassette trough to collect any particles
that have adhered to it.
19
Estimation of Collected Particle Load
Direct microscopy enables the analyst to estimate the concentration of
particles in the sampled area. Counting and quantification of the collected
particles accomplish this. This estimation is conducted by counting calibrated
cross-sections of the deposited sample trace. The number and type of particles
estimated per cubic meter of air is calculated based on the a) length of the
deposition trace, b) length of trace actually examined, c) volume of air
collected, and d) number of particles counted.
The Via-Cell Sampler particle deposition area is approximately 0.9 mm wide by
14.4mm long yielding an approximate area of 12.96 mm2. The width of the
deposition trace may vary slightly in particle density from the middle to
outer edges of deposition. For this reason, using the deposition trace area is
not recommended for direct calculation of particle concentrations. The
recommended procedure for calculating particle concentrations is based on
using the Via-Cell Sampler trace length and microscope field diameter. One
field of view counted is defined as the calibrated diameter of the microscope
field of view (in mm) covering one cross-sectional pass or “traverse” across
the sample deposition trace. A typical sample preparation and microscopic
counting procedure is illustrated in Figure 5.
Figure 5 ILLUSTRATION OF THE Via-Cell® COUNTING PROCEDURE
Microscope slide
Analysis starts at 1st traverse – completes and moves to 2nd traverse
Via-Cell slide
Sample particulate
20
The calculation of particle concentration per cubic meter of air can be performed by using the following equations:
STEP 1 Determine the actual air volume collected in cubic meters (m3) using
the following calculation:
Air volume (m3) = (Sampling rate (liters per minute) / 1000) x Number of
minutes
STEP 2 Determine the length of sample trace counted based on the microscope
field of view and number of fields of view counted. It will be necessary to
accurately calibrate and measure the diameter of the microscope field of view
using a stage micrometer slide. Remember, each microscope is different, and
each different combination of ocular and objective lens must be calibrated
separately. Stated lens magnifications are rarely precise. The microscopist
should then record the number of complete traverses examined across the width
of the deposition trace and use the formula given below to calculate the
actual length of the deposition trace examined.
Trace Length Counted (mm2) = Microscope field diameter (mm) x number of
traverses
STEP 3
The concentrations of particles (cts/m3) can then be determined by using the
equation below.
Trace length (14.4mm)
1
Cts / m3 = ____ x x # of particle counts
Total length of trace counted
Air Volume(m3)
(From Step 2)
(From Step 1)
21
Mold Spore Example Calculation
Microscope field diameter at (900X) = 0.240 mm
Number of traverses
= 10
Sample volume (15 lpm @10 minutes) = (15 / 1000) x 10 = 0.150m3
Mold spore counts
= 50
14.4mm _____ 0.240 x 10
1
14.4
x __ x 50 = x 50 = 2000 ct/m3
0.15
0.36
MICROSCOPIC COUNTING RECOMMENDATIONS
Particle Type
Pollen —
Counting Recommendation
Entire trace or 100 grains (whichever comes first) should be examined at a
minimum magnification of 200X. Identification and speciation should be
performed at minimum magnification of 400X.
Mold Spores —
A minimum of 15% of the entire trace should be examined or a minimum of 100
mold spores counted (whichever comes first). Identification and speciation
should be performed at minimum
magnification of 400X.
Fibers — Other Aerosols —
The entire trace or 100 fibers, (whichever comes first) should be examined at
a minimum magnification of 200X.
Skin cell fragments, combustion emissions, insect parts –A minimum of 10% of
the entire trace should be examined or a minimum of 100 particles counted
(whichever comes first).
Storage & Operating Conditions
This product should be stored at room temperature, between 60-82°F (15-28°C).
Do not use product at temperatures below 32°F (0°C). If product has been
exposed to freezing temperatures immediately before sampling, it is
recommended to let the product acclimate to the sampling environment before
use.
Ordering Information
Product Number VIA010 AOCCAL AOC-WS10 Z-LITE-IAQ ZBP-200 ZBP-200-CAL 510-H
Field Equipment
Description Via-Cell Cassettes, 10/pk In-Line Calibration Adapter Inner Wall
Sampling Adapter Zefon Z-Lite IAQ Pump Zefon Bio-Pump® Plus TSI 4046
Calibrator Kit for Bio-Pump Bios DryCal® Defender Calibrator
Laboratory Equipment
Product Number ZA0046 ZA0050 ZA0051 ZA0052 ZA0054 ZA0055 ZA0056 ZA0058
Description Cassette Opener Particle Spreader, “T” shaped, sterile Syringe, sterile, 3cc Alcohol wipes, 200/bx Forceps, self closing, each Water, sterile, 100cc, screw cap container Via-Cell Linking Solution Via-Cell Blue Stain
5350 SW 1st Lane, Ocala, FL 34474 · www.zefon.com Phone:
800-282-0073 ·
352-854-8080 · Fax: Fax:
352-854-7480
© Copyright 2016 Zefon International, Inc. Via-Cell, Air-O-Cell, and BioPump
are registered trademarks of Zefon International.