miris Ultransonic Processor User Manual

: June 8, 2024
: MIRIS

miris Ultransonic Processor

INSTRUCTIONS

READ THE MANUAL BEFORE USING THE INSTRUMENT

PREFACE
Thank you for choosing the Miris Ultrasonic Processor.
Please read this manual carefully before starting to use the instrument.
Miris Ultrasonic Processor is used to prepare milk samples for macronutrient analysis using Miris HMA™ – Human Milk Analyzer. By the application of high- frequency ultrasonic waves, the milk sample will be homogenised in a simple and convenient manner.
© Copyright 2022 Miris AB, all rights reserved. This manual is protected by copyright. Distribution and copying in any form of the content is prohibited without the explicit and written consent of Miris AB.

Manufacturer contact information

address
- MIRIS AB
- Danmarksgatan 26
- 753 23 Uppsala
- Sweden
web
- www.MirisSolutions.com
e-mail
- info@MirisSolutions.com
telephone
- +46 18 14 69 07

Document information

name
- Miris Ultrasonic Processor User Manual
ID
- DOC-865
revision
- 1
issue date
- 2022-01-25
article number
- 2022-01-25

Product information

name
- Miris Ultrasonic Processor
article number
- 08-04-201
standard
- 2014/30/EU Electromagnetic
- Compatibility Directive
- 2014/35/EU Low Voltage Directive
- 2011/65/EU RoHS 2 Directive

IMPORTANT USER INFORMATION

INTENDED USE OF THE MIRIS ULTRASONIC PROCESSOR
Miris Ultrasonic Processor reduces the particle size distribution in milk. This device is intended for use in laboratories by laboratory personnel.

WARRANTIES AND DUTIES

By operating this Miris Ultrasonic Processor, the USER and MIRIS agree to the following responsibilities, which constitute contractual warranties and conditions between MIRIS and
USER for the maximum benefit and usefulness of the Miris Ultrasonic Processor.

MIRIS AB WARRANTS THAT IT:

Knows of no defects in the construction of the Miris Ultrasonic Processor or its materials used
Will replace or repair the Miris Ultrasonic Processor according to the guarantee in the product warranty

USER WARRANTS THAT:

The Miris Ultrasonic Processor will be used according to the instructions given in the user manual
The Miris Ultrasonic Processor will not be altered without written approval of MIRIS AB
MIRIS will be notified immediately if any injury occurs in any association with the Miris Ultrasonic Processor, and will be allowed prompt and thorough examination of the Miris Ultrasonic Processor in question
MIRIS will not be held responsible in cases of injury arising from use of the Miris Ultrasonic Processor:
1. When the Miris Ultrasonic Processor is not used according to the instructions in this manual
2. When MIRIS is not notified within 5 days of said injury

SAFETY INFORMATION

When mounting the probe, always clamp the converter housing.
Never clamp the probe.
Make sure the Ultrasonic Processor is properly grounded via a 3-prong outlet.
High voltage is present in the power supply.
Do not remove the cover.
Service can only be performed by an authorized service technician.
Ask your local distributor or contact Miris.
Never operate the power supply unless it is connected to the converter.
Never secure anything to the probe.
Never touch a vibrating probe.
Never allow a probe to vibrate in air for more than 10 seconds.
Always use input voltage 110/240 V , 50/60 Hz.

INSTALLATION

Figure 1. The Miris Ultrasonic Processor.

Sound reducing cabinet
Display and keypad

THE INSTRUMENT

Figure 2. Inside the Sound reducing cabinet.

Height adjustment knob
Convertor housing
Probe

Figure 3. Display and keypad.

Display with current settings
Probe output power indicator
Digit input 1-9
Clear
Enter/Review
Start/Stop
On
Off
Timer
Pulse
Amplitude

ELECTRICAL REQUIREMENTS

The Miris Ultrasonic Processor requires a fused, single phase 3-terminal grounding type elec-trical outlet capable of supplying 50/60 Hz at 100 Volts, 115 Volts, 220 Volts, or 240 Volts, depending on the voltage option selected. For power requirements, check the label on the back of the unit.

Probe processing capacity
For efficient homogenization, it is important to not exceed the processing capacity of the probe.
The 3 mm diameter probe is for milk volumes 3-10 ml.

SETTINGS

TIME

Processing time for human milk samples is 1.5 s/ml milk.
Calculate sample processing time in seconds by multiplying sample volume in ml by 1.5
Set sample processing time by pressing [Timer]
Use the key pad to enter sample processing time given as hours, minutes, seconds, in the format H:MM:SS
Press [Enter] to confirm
The set time is displayed

PULSE

Pulsating mode, Pulse, should be turned off.
Press [Pulse]
Press [Clear] to erase current setting
Press [Enter] to confirm
Pulse is now turned off, shown on the display as —

AMPLITUDE

Amplitude should be set to 75%.
Press [Ampl]
Use the key pad to enter 075
Press [Enter] to confirm
Amplitude is now set to 75%, shown on the display

OPERATING THE MIRIS ULTRASONIC PROCESSOR

This chapter describes how to use the Miris Ultrasonic Processor.
See Chapter 1 for start-up procedure and Chapter 2 for settings.
For information on use and handling of human milk and the Miris HMA™, refer to instructions in the Miris HMA™ User Manual.

STANDARD OPERATING PROCEDURE

Equipment Consumables

Miris Ultrasonic Processor| Miris Cleaner™ Emery cloth Sample container

Table 1. Standard Operating Procedure (SOP) for processing milk using Miris Ultrasonic Processor.

Set- up Prepare working solution of Miris Cleaner™
Turn on the Miris Ultrasonic Processor by pressing [I]

Instrument preparation
See Chapter 2 and Chapter 4| Check that amplitude is set to 75% and pulsating mode is turned off Set the processing time (1.5 s/ml) according to sample volume Make sure that the Miris Ultrasonic Processor probeis clean and that the tip is evenly polished

Sample processing

(For instructions on human milk preparation and handling, see the Miris HMA™ User Manual)
Use a sample container which will give a sample depth of at least 2 cm, see Figure 4, allowing some extra space for sample movement
If using conical tubes, a tube holder i necessary to keep the tube upright and stable. If using a flat bottom or
skirted tube, a tube holder or tube rack may not be necessary.
Place the container with the sample in the Miris Ultrasonic Processor Sound reducing cabinet
Adjust the probe height using the Height adjustment knob
Immerse the probe into the sample
Note! If the probe is lowered to an insufficient depth the sample will foam and/or splash, which should be avoided
Centre the probe in the container
Close the door of the Sound reducing cabinet
Press Start [Start/Stop]
The processing can be stopped at any time by pressing [Start/Stop] again
After the processing is finished, remove the sample
(The sample is now ready for analysis with Miris HMA™ Calibration 1 – Homogenized milk)

Cleaning /Full instructions in Chapter 4

After each use of the Miris Ultrasonic Processor, wipe the probe with a tissue
At the end of the day, wipe the probe and all contaminated areas with a cloth dampened with Miris CLEANER™
Polish the probe tip with a piece of emery cloth
Turn off the Miris Ultrasonic Processor by pressing [O]

Figure 4. Sample container placement.

CLEANING ROUTINES

Miris Cleaner™ and emery cloth are necessary for proper maintenance of the instrument.

REQUIRED CONSUMABLES

MIRIS CLEANER™
Description: Cleaning agent (liquid concentrate).
Application: For cleaning of the Miris Ultrasonic Processor probe and surfaces.
Storage: Store the concentrate dark, at room temperature (20-30°C), sealed in the container in which it is supplied. Store the diluted solution in glass or plastic containers, out of direct sunlight. Keep away from foodstuff and animal feed.
Instructions: Dilute 50 ml concentrate (1 tube) with 950 ml distilled or deionized water and mix. Follow the instructions in the section Cleaning of the instrument.
The estimated time for preparation is 5 minutes for mixing the ingredients. The ready to use dilution should have a faint odor (soapy, chemical) and no color.

Materials provided:
Liquid concentrate in 50 ml container
Materials required but not provided: 1000 ml glass or plastic container 950 ml distilled or deionized water

Do not use if the liquid is cloudy or precipitations are visible.
Use before: Expiry date for unopened tubes is 1 year from production date.
Use solution within 3 months of preparation.
Environment and health: Small spillages may be flushed away with plenty of water to a drain or sewer. Miris Cleaner™ is biodegradable.
Ready biodegradability by OECD 301E (ISO method 7287 – 1986(E)).
Non-hazardous. In case of contact with eyes, rinse immediately with plenty of clean, flowing water. Seek medical attention if there is persistent irritation.
In case of contact with skin, rinse immediately with plenty of clean, flowing water.
If ingested, drink copious amounts of fresh water and seek immediate medical attention.
Do not promote vomiting.

EMERY CLOTH
Description: Fine grit emery cloth.
Application: For polishing the Miris Ultrasonic Processor probe tip.

CLEANING OF THE INSTRUMENT

To maintain the homogenization efficiency of the Miris Ultrasonic Processor, it is important to clean and polish the probe after each use. Homogenization efficiency is determined by the capability of the probe to transmit energy into the milk, and this capability will degrade in proportion to the degree of roughness of the tip surface. Any erosion of the probe tip, visible as dark spots or a ring round the outer edge of the tip, must therefore be removed by careful polishing with
fine grit emery cloth. Polish off the eroded part only and no more, or the probe will wear out quicker.

PROBE CLEANING

Wipe off the probe after every milk sample using a tissue.
Clean the probe with a cloth dampened with Miris Cleaner™ after finishing for the day.
Polish the tip (the flat end) of the probe with an emery cloth after each use.
Make sure to polish the probe evenly, avoiding beveled edges, and to complete- ly remove any milk residues, see Figure 5.

Figure 5. Polished probe tip.

INSTRUMENT SURFACE

Wipe spillages immediately. Clean the instrument surface and inside using a cloth dampened with Miris Cleaner™.
If necessary, a mild disinfectant can be used to clean the probe and the instrument surfaces.

TROUBLESHOOTING AND PROBLEM SOLVING

If any problem occur that you are unable to solve by referring to this manual, please contact your local distributor or Miris. When doing so, please include the serial number of your instrument. The serial number is printed on the label placed at the back of the Miris Ultrasonic Processor.

TROUBLESHOOTING

Table 2. Trouble shooting guide.

Error| Probable cause| Action| If the error persists
---|---|---|---
Error message “OVERLOAD” or the instrument stops working| The unit was plugged into an electrical outlet that provides a different voltage from that required
The probe is not secured properly
A fuse has failed| Check the voltage on the back of the instrument
Make sure the probe is properly attached.
Use two wrenches, hand-tightening is not sufficient| Contact Miris (support@miris.se) or your local distributor
| | Restart the instrument|

ABOUT MIRIS ULTRASONIC PROCESSOR

TECHNICAL SPECIFICATIONS

Table 3. Technical specifications of the Miris Ultrasonic Processor

Dimensions (HxWxL) 64 x 32 x 42 cm
Weight 11 kg
Power supply 110 V/240 V, 50/60 Hz
Net power output 130 Watts
Frequency 20 kHz
Display LCD
Probe Titanium alloy Ti-6Al-4V
Probe size Length 138 mm, diameter Ø 3 mm
Probe processing capacity 3-10 ml
Standards
- 2014/30/EU Electromagnetic Compatibility Directive
- 2014/35/EU Low Voltage Directive
- 2011/65/EU RoHS 2 Directive
- UL/CSA/EN 61010-1:2013 3rd Edition

WORKING PRINCIPLE OF THE INSTRUMENT

PRINCIPLE OF OPERATION
The power supply converts 50/60 Hz voltage to high frequency energy, which is transmitted as mechanical vibrations from the converter. The vibrations are intensified by the probe and make the probe tip oscillate in the length direction, creating pressure waves. Due to these pressure waves, microscopic air bubbles are created and disrupted within microseconds, a phenomenon called cavitation. When the air bubbles disrupt, the release of kinetic energy will break large fat globules into smaller ones. Small fat globules have less creaming tendencies and stay homogenously distributed in the milk.

MILK HOMOGENISATION
Fat and proteins are the main structural elements of the milk system, and their chemical characteristics affect the milk as a solution.
Milk fat is considered an oil-in-water emulsion, with fat droplets covered by a hydrophilic membrane.
The milk proteins are a colloidal solution or dispersion in the milk plasma.
Time, temperature and gravitation are some parameters that affect the chemical characteristics of these components, i.e.
the milk fat will separate when allowed to stand, known as creaming [1].
Creaming of milk fat is faster at low temperatures due to cold agglutination [2].

Miris Ultrasonic Processor homogenizes the milk using the cavitation phenomenon. Cavitation is the formation and collapse of air bubbles caused by pressure fluctuations. The collapse of air bubbles releases shock waves, causing damage to the surrounding particles [1].
The energy output from the Miris Ultrasonic Processor is approximately 20 J/s per ml of milk.

In milk, cavitation causes disruption of milk fat globules into small-er ones, and proteins are adsorbed onto the fat droplets, which improves the stability of the fat globules [1]. Ultrasound is more efficient when combined with heating [2].

REFERENCES

P. Walstra, J. Wouters, T. Geurts. Dairy Science and Technology, second edition, Taylor and Francis Group, Boca Raton, 2006.
M. F. Ertugay, M. Sengul, M.Sengul, “Effect of Ultrasound Treatment on Milk Homogenization and Particle Size Distribution of Fat”, Turkish Journal of Veterinary Animal Science, vol. 28, pp. 303-308, 2004.

www.MirisSolutions.com