Leica Biosystems HistoCore MULTICUT Rotary Microtome Instructions
- June 13, 2024
- Leica Biosystems
Table of Contents
Leica Biosystems HistoCore MULTICUT Rotary Microtome
Product Information
The HistoCore MULTICUT is a rotary microtome designed for cutting thin sections of tissue samples for microscopic examination. It is manufactured by Leica Biosystems Nussloch GmbH, a company based in Nussloch, Germany.
The instrument is equipped with various components and features that allow for precise and efficient sectioning of tissue samples. It has a clamping system, a base plate, and other instrument components that ensure stability and accuracy during the cutting process.
The instrument specifications and technical data are provided in the user manual, which can be found in this document.
Product Usage Instructions
Before using the HistoCore MULTICUT, please read the user manual carefully and familiarize yourself with the instrument’s operation and safety guidelines.
To set up the instrument:
- Ensure that you have all the necessary accessories and components.
- Place the instrument on a stable surface.
- Check the instrument serial number and year of manufacture on the nameplate located on the back of the instrument.
- Refer to the assembly instructions provided by Leica Microsystems Ltd. Shanghai for proper assembly of the instrument components.
To operate the instrument:
- Ensure that the instrument is properly set up and all components are securely in place.
- Refer to the user manual for detailed instructions on operating the rotary microtome.
- Follow the recommended safety precautions to prevent any accidents or injuries during use.
- Adjust the clamping system of the clamp mount on the base plate according to the desired cutting thickness.
- Place the tissue sample on the base plate and position it correctly.
- Engage the cutting mechanism and carefully rotate the microtome wheel to cut thin sections of the tissue sample.
- Collect the sections and proceed with further processing or examination as required.
If you encounter any issues or difficulties while using the instrument, refer to the troubleshooting section of the user manual for possible solutions.
Optional accessories are available for the HistoCore MULTICUT, which can enhance its functionality and performance. Refer to the user manual for more information on these accessories.
HistoCore MULTICUT
Rotary Microtome
Instructions for Use English Order No.: 14 0518 80101 – Revision J Always keep
this manual with the instrument. Read carefully before working with the
instrument.
Version 1.7, Revision J – 01.2023
Important Note
The information, numerical data, notes and value judgments contained in this
manual represent the current state of scientific knowledge and state-of-the-
art technology as we understand it following thorough investigation in this
field. We are under no obligation to update the present manual periodically
and on an ongoing basis according to the latest technical developments, nor to
provide our customers with additional copies, updates etc. of this manual.
To the extent permitted in accordance with the national legal system as
applicable in each individual case, we shall not be held liable for erroneous
statements, drawings, technical illustrations etc. contained in this manual.
In particular, no liability whatsoever is accepted for any financial loss or
consequential damage caused by or related to compliance with statements or
other information in this manual. Statements, drawings, illustrations and
other information regarding the contents or technical details of the present
Instructions for Use are not to be considered warranted characteristics of our
products.
These are determined only by the contract provisions agreed between ourselves
and our customers. Leica reserves the right to change technical specifications
as well as manufacturing processes without prior notice. Only in this way is
it possible to continuously improve the technology and manufacturing
techniques used in our products.
This document is protected under copyright laws. All copyrights to this
documentation are held by Leica Biosystems Nussloch GmbH. Any reproduction of
text and illustrations (or of any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods including any electronic systems and
media requires express prior permission in writing by Leica Biosystems
Nussloch GmbH.
For the instrument serial number and year of manufacture, please refer to the
nameplate on the back of the instrument.
Leica Biosystems Nussloch GmbH Heidelberger Strasse 17 – 19 D-69226 Nussloch Germany
Tel.: Fax: Web:
+49 – (0) 6224 – 143 0 +49 – (0) 6224 – 143 268 www.LeicaBiosystems.com
Assembly contracted to Leica Microsystems Ltd. Shanghai
HistoCore MULTICUT
Version 1.7, Revision J
Important Information 1
Important Information
Symbols used in the text and their meanings
Danger: Indicates an imminently hazardous situation which, if not avoided,
will result in death or serious injury. Warning: If this danger is not
avoided, then this may result in death or serious injury.
Caution: Indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury. Note: Indicates a situation with the
potential for property damage which, if not avoided, could result in damage to
the machine or something in its vicinity.
Tip: Gives you tips to simplify your workflow.
1 “Fig. 7 – 1”
Item numbers for numbering illustrations. Numbers in red refer to item numbers
in illustrations.
Manufacturer
Order number Serial number Date of manufacture
Observe the Instructions for Use.
Caution, consult the instructions for use for cautionary information.
In vitro diagnostics (IVD) medical device The CE marking is the manufacturer’s
declaration that the medical product meets the requirements of the applicable
EC directives and regulations.
The Country of Origin box defines the Country where the final character
transformation of the product has been performed.
HistoCore MULTICUT
7
1 Important Information
The UKCA (UK Conformity Assessed) marking is a new UK product marking that is
used for goods being placed on the market in Great Britain (England, Wales and
Scotland). It covers most goods which previously required the CE marking.
The UK Responsible Person acts on behalf of the non-UK manufacturer to carry
out specified tasks in relation to the manufacturer’s obligations.
Symbol for labeling electrical and electronic equipment in accordance with
Section 7 of the German Electrical and Electronic Equipment Act (ElektroG).
ElektroG is the law on the bringing into circulation, return and
environmentally compatible disposal of electrical and electronic equipment.
The CSA test mark means that a product has been tested and fulfills the
applicable safety and/or performance standards, including the relevant
standards defined or administered by the American National Standards Institute
(ANSI), Underwriters Laboratories (UL), the Canadian Standards Association
(CSA), the National Sanitation Foundation International (NSF) and others.
Symbol for alternating current
PE terminal
Power on
Power off
The package contents are fragile and must be handled with care.
The package must be kept in a dry environment.
Maximum of 3 stacked layers
Indicates the correct upright position of the package.
Indicates the temperature range permitted for transporting the package.
Minimum -29 °C Maximum +50 °C
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Version 1.7, Revision J
Important Information 1
Indicates the temperature range permitted for storing the package. Minimum +5
°C Maximum +50 °C
Indicates the humidity range permitted for storing and transporting the
package. minimum 10 % r.H. maximum 85 % r.H
In the Shockwatch system, the shock dot shows impacts or shocks that are above
a specified intensity through red colouration. Exceeding a defined
acceleration (g value) causes the indicator tube to change colour.
Indicates the item can be recycled where correct facilities exist.
1.2 Instrument type
All information provided in these Instructions for Use applies only to the
instrument type indicated on the title page. A nameplate indicating the
instrument serial number is attached at the back side of the instrument.
1.3 Intended purpose
The HistoCore MULTICUT is a semi-motorized (motorized specimen feeding),
manually operated rotary microtome specifically designed for creating thin
sections of formalin fixed, paraffin embedded human tissue specimens of
varying hardness used for histological medical diagnosis by a pathologist,
e.g. for cancer diagnosis. It is intended for sectioning soft and hard human
specimens, as long as they are suitable for being cut manually. The HistoCore
MULTICUT is designed for in vitro diagnostic applications.
Warning Any use other than the intended use. Serious injury of persons and/or
damage to specimen.
· Follow the intended use described in the Instructions for Use. Any other use
of the instrument will be considered as improper use.
1.4 Qualification of personnel
· The HistoCore MULTICUT must be operated by trained laboratory personnel
only. The instrument is intended for professional use only.
HistoCore MULTICUT
9
1 Important Information
· All laboratory personnel designated to operate this instrument must read
these Instructions for Use carefully and must be familiar with all technical
features of the instrument before attempting to operate it.
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Version 1.7, Revision J
Safety 2
2. Safety
2.1 Safety notes
The Instructions for Use include important information related to the
operating safety and maintenance of the instrument. The Instructions for Use
are an important part of the product, and must be read carefully prior to
startup and use and must always be kept near the instrument. This instrument
has been built and tested in accordance with the safety regulations for
electrical measuring, control, regulating and laboratory instruments. To
maintain this condition and ensure safe operation, the user must observe all
notes and warnings contained in the Instructions for Use. The safety and
caution notes in this chapter must be observed at all times. Be sure to read
these notes even if you are already familiar with the operation and use of
other Leica Biosystems products. The Instructions for Use must be
appropriately supplemented as required by the existing regulations on accident
prevention and environmental safety in the operator`s country.
Warning
Protective devices or safety accessories provided by manufacturer have been
removed or modified. Serious injury of persons and/or property damage
including specimen damage.
· Never remove or modify any protective devices of the instrument or any
accessories. Only service personnel qualified by Leica Biosystems may repair
the instrument and access the instrument’s internal components.
· Before working with the instrument always assure that all protective devices
and safety accessories are in place and fulfill their purpose properly.
Tip
For current information about applicable standards, please refer to the CE
Declaration of Conformity and UKCA Certificates on our Internet site:
http://www.LeicaBiosystems.com
The electromagnetic environment should be evaluated prior to operation of the
instrument. Do not use this instrument in close proximity to sources of strong
electromagnetic radiation (e.g. unshielded intentional RF sources), as these
can interfere with the proper operation. The instrument complies with the
emission and immunity requirements described in the IEC 61326-2-6.
2.2 Warnings
The protective devices installed in this instrument by the manufacturer only
constitute the basis for accident prevention. Operating the instrument safely
is, above all, the responsibility of the owner, as well as the designated
personnel who operate, service or clean the instrument. To ensure trouble-free
operation of the instrument, be sure to comply with the following instructions
and warnings.
HistoCore MULTICUT
11
2 Safety
2.2.1 Markings on the instrument itself
Warning
Failure to follow the correct operating instructions (as defined in the
Instructions for Use) of the corresponding markings and warning triangles on
the instrument. Serious injury of persons and/or damage to instrument,
accessories or specimen.
· Pay attention to the markings on the instrument and strictly follow
operating instructions described in the Instructions for Use when operating or
replacing the item marked.
2.2.2 Transport and installation
Warning
Accessories/instrument falling out of the package during unpacking. Serious
injury of persons and/or property damage.
· Be careful when unpacking the instrument. · Once unpacked, the instrument
must be moved only in an upright position. · Thoroughly follow the
instructions given in the Unpacking Instructions attached at the outside of
the package or the actions described in the Instructions for Use.
Warning
Instrument is lifted inappropriately. Serious injury of persons and/or
property damage.
· When lifting the instrument touch it only at the lifting points described in
the Instructions for Use and Unpacking Instructions (base plate at the front
and under the instrument on the back).
· Never lift the instrument by the handwheel grip, electric coarse feed wheel,
or object head. · Always remove the section waste tray before transporting the
instrument.
Warning
Careless moving of the instrument. Serious injury of hands and/or fingers due
to getting crushed between instrument and working surface.
· When moving the instrument, touch it only at the lifting points described in
the Instructions for Use and Unpacking Instructions (base plate at the front
and under the instrument on the back).
· Thoroughly pay attention that hands do not get between instrument and
working surface.
Warning
Instrument not positioned safely with all 4 feet on an appropriate laboratory
table. Serious injury of persons and/or property damage.
· Put the instrument only on a stable, vibration free laboratory table with
horizontal, flat table top. Ground needs to be vibration-free as far as
possible.
· Always make sure that all 4 feet are positioned completely on the laboratory
table. · If the instrument has been potentially moved by other persons (for
example for servicing) always
double-check correct positioning.
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Safety 2
Warning Condensation inside the instrument due to exposure to extreme
differences in temperature and high air humidity. Damage of the instrument.
· Always make sure that for both storage and operation appropriate climate
conditions are met. See the section of Technical data ( P. 27 3.3 Technical
Data).
· After transporting the instrument, wait at least two hours to allow the
instrument to adopt the ambient temperature before turning it on.
Warning Knife/blade holder falling off the instrument. Serious injury of
persons and/or property damage.
· If the knife/blade holder is not fixed, for example, during installation or
cleaning, pay special attention that the knife/blade holder is not falling
off.
· Whenever possible, fix the knife/blade holder to avoid falling off. · For
installation or maintenance purposes remove the knife/blade holder from the
knife holder base
to avoid accidental falling off.
Warning Section waste tray falling down after it has been detached. Injury of
persons.
· Work especially carefully when detaching the section waste tray and put it
in a safe place.
Warning Oil is spilled and not cleaned-up immediately. Serious injury of
persons, for example, by slipping and getting in contact with dangerous parts
like the knife/blade of the instrument.
· Always make sure that no oil is spilled. · If oil has been spilled, clean it
up immediately, thoroughly and completely.
Warning Paraffin waste falls on the floor and is not cleaned-up. Serious
injury of persons, for example, by slipping and stumbling into the
knife/blade.
· Always clean up paraffin waste before it spreads, becomes slippery, and
becomes a hazard. · Wear appropriate shoes.
Warning Wrong voltage of power supply selected at the voltage selector.
Instrument damage, delay of specimen processing.
· Assure to select the voltage fitting to your mains voltage before plugging
in the instrument. · When changing the voltage selector setting, the
instrument must be disconnected from the mains.
HistoCore MULTICUT
13
2 Safety
Warning Connecting the instrument to a non-grounded socket, or use an
extension cord. Risk of electrical shock which causes injury to persons, or
delayed diagnosis.
· The instrument must be connected to a grounded socket. · Do not use an
extension cord.
Caution Accessories/components loosening/damaged during transportation.
Property damage or delayed diagnosis.
· The packaging has the ShockDot Impact Indicator, that indicates improper
transport. When the instrument is delivered, check this first. If the
indicator is triggered, the package was not handled as prescribed. In this
case, please mark the shipping documents accordingly and check the shipment
for damage.
2.2.3 Operating the instrument
Danger Explosive hazard. Death or serious injury of persons, and/or property
damage.
· Never operate the instrument in rooms with an explosive hazard.
Warning Personnel with insufficient qualification working with the instrument.
Serious injury of persons and/or specimen damage can occur when specimen
approaches knife/ blade due to inappropriate operator actions, for example,
because object head may drop onto knife holder when handwheel is unlocked.
· Always make sure that only laboratory personnel with dedicated and
sufficient training and qualification operates the instrument.
· Always make sure that all laboratory personnel designated to operate this
instrument has read these Instructions for Use carefully and is familiar with
all technical features of the instrument before start working with it.
Warning Protective devices or safety accessories provided by manufacturer have
been removed or modified. Serious injury of persons and/or property damage
including specimen damage.
· Never remove or modify any protective devices of the instrument or any
accessories. Only service personnel qualified by Leica Biosystems may repair
the instrument and access the instrument’s internal components.
· Before working with the instrument always assure that all protective devices
and safety accessories are in place and fulfill their purpose properly.
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Version 1.7, Revision J
Safety 2
Warning
Personal protective equipment is not used. Injury of persons.
· When working with microtomes, personal safety precautions must always be
taken. It is mandatory to wear work safety shoes, safety gloves, a mask and
safety goggles.
Warning
Knives or blades are handled and/or disposed in an inappropriate manner.
Serious injury of persons due to contact with extremely sharp knife/blade.
· Work especially thoroughly and carefully when handling the knife/blade. ·
Always wear dedicated clothes (including cut-resistant gloves) when handling
knife/blade. · Dispose knives and blades always in a safe place (for example,
dedicated knife case) and an
appropriate manner that ensures that people cannot get hurt. · Never place a
knife anywhere with the cutting edge facing upwards and never try to catch a
falling knife. · Always cover the edge of the knife/blade with the safety
guard before clamping the specimen.
Warning
Knife/Blade was not removed when knife/blade holder was uninstalled from the
microtome. Serious injury of persons due to contact with extremely sharp
knife/blade.
· Before uninstalling the knife/blade holder from the microtome always make
sure to detach the knife/blade wearing cut-resistant gloves and store the
knife/blade in a safe place.
Warning
The operator grabs into the knife/blade due to inappropriate working
procedure. Serious injury of persons when putting the specimen in place if
knife/blade has been installed before.
· Prior to uploading the specimen on the microtome, make sure that the cutting
edge has been covered by the safety guard and the hand wheel locking mechanism
has been activated. Once the operator wants to upload the specimen as well as
inserting a knife/blade, always upload the specimen block before mounting and
clamping the knife/blade.
Warning
The operator installs two knives/blades on the knife/blade holder. Serious
injury of persons due to contact with extremely sharp knives and/or blades.
· Do not clamp two knives/blades on the knife holder/blade holder. Install the
knife/blade in the center of the knife/blade holder. Knives/blades must not
exceed the edge of the knife/blade holder.
HistoCore MULTICUT
15
2 Safety
Warning
Too thick or thin blade used in the two-in-one blade holder. Sample impaired.
· Use a blade with a maximum thickness of less than 0.322mm and a minimum
thickness of more than 0.246mm.
· The recommended compatible blade is listed in Optional Accessories ( P. 61
6. Optional Accessories).
Warning
The blade is not installed completely parallel to the upper edge of the
pressure plate due to excess paraffin, uncleaned inserts, etc.. When using the
lateral movement function, failure to install the blade parallel to the
pressure plate may result in poor sectioning results. For example, if a
section is too thick or thin, chattering within the section, in the worst-case
scenario, may damage the specimen.
· Do not continue the sectioning when getting unsatisfying section results. ·
Re-install the blade and make sure that it is parallel to the upper edge of
the pressure plate. · Always check the parallelism between the blade and
pressure plate after moving the blade using
the lateral movement function. · Ensure there is no paraffin waste and the
insert is clean before use.
Warning
The knife/blade is installed before the knife/blade holder and the knife/blade
holder base are installed on the instrument. Serious injury of persons due to
contact with extremely sharp knives and/or blades.
· Prior to inserting the knife/blade, both the knife/blade holder and the
knife/blade holder base must be installed on the microtome.
Warning
Knife/blade is not covered with the appropriate guard when not sectioning
specimen. Serious injury of persons.
· Always cover the knife/blade edge with the appropriate guard prior to any
manipulation of knife/ blade or specimen clamp, prior to changing the specimen
and during all work breaks.
Warning
Knife/blade stored in inappropriate manner. Serious injury of persons, for
example due to unexpected fall off.
· Always store the knife/blade in an appropriate place when not using it, for
example, store it in the dedicated knife case.
· Never place a knife anywhere with the cutting edge facing upwards and never
try to catch a falling knife.
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Version 1.7, Revision J
Safety 2
Warning
Uploading or unloading the specimen on the microtome without appropriate
clothes and in an inappropriate manner. Operator can get cut and injured which
can lead to serious consequences.
· Always use cut resistant safety gloves when handling the specimen within the
microtome. · Lock the handwheel and cover the knife edge with the safety guard
prior to any manipulation of
the specimen clamp and prior to changing the specimen.
Warning
Specimen is oriented in retraction phase. Specimen damage due to re-orienting
the specimen in retraction phase.
· Specimen blocks must not be oriented during the retraction phase. If a block
is oriented during retraction, the block will advance by the retraction value
plus the selected section thickness before the next section. This may cause
damage to both specimen and knife/blade.
Warning
Working with the instrument and brittle specimen without appropriate
protective clothes. Serious injury of persons by splinters while cutting
brittle specimen.
· Always wear appropriate protective clothes (including safety goggles) and
work especially carefully when cutting brittle specimen.
Warning
Wrong fine adjustment of the force balance. Serious injury of the operator due
to contact with knife and/or specimen damage.
· Before working with the instrument, always double-check that the fine
adjustment of the force balance is properly set.
· If fine adjustment is not properly set, do not work with the instrument but
perform a readjustment. For details, refer to chapter “Fine adjustment of the
force balance”.
· Especially after changing accessories at the object head, perform a fine
adjustment of the force balance immediately.
Warning
Insufficient frame conditions for sectioning. Specimen damage or poor
sectioning results, for example, sections alternating in thickness,
compressed, folded or stripped sections.
· Do not continue sectioning if you recognize insufficient sections results. ·
Make sure that all prerequisites for appropriate sectioning are met. Refer to
the troubleshooting
section of these Instructions for Use for further details. · If you do not
have sufficient knowledge in regards of troubleshooting insufficient
sectioning
results consult persons who do have that knowledge, for example, application
experts of Leica Biosystems.
HistoCore MULTICUT
17
2 Safety
Warning Wheel turn direction of electric coarse feed incorrectly selected.
Specimen damage.
· Always assure that you have selected the correct turn direction of the
coarse feed before turning the wheel.
Warning Turning the handwheel counterclockwise. People injury/Damage to
specimen.
· Do not turn the handwheel counterclockwise, which may lead to handwheel
locking mechanism malfunction.
Warning The handwheel is unlocked and the object head drops into knife/blade
holder. People injury/Damage to specimen.
· Except sectioning phase, the handwheel must be locked at all times.
Warning The rotation speed of the handwheel does not suit the hardness of the
specimen. Instrument damage and possibly specimen damage.
· The rotation speed of the handwheel must be adapted to suit the hardness of
the specimen. For harder specimens, use a slower speed.
Warning In the manual sectioning mode, the user cuts the specimen block by
rotating the handwheel at a very high speed. It may lead to poor sectioning
quality and even damage the specimen.
· In the manual sectioning mode, the rotation speed of the handwheel shall be
no more than 60 rpm.
Warning The handwheel brake lever is not used properly, and the handwheel
cannot be braked. Serious injury of instrument/persons, or specimen damage.
· The handwheel brake lever must be exactly in the locked position. If the
handwheel brake lever is moved beyond this point, it is possible that the
handwheel is no longer braked.
Warning When doing fast manual trimming and fingers are put between the
specimen and the knife/blade after the handwheel is released. Operator can get
cut and injured due to the turning of the handwheel when it is unlocked.
· Do not put fingers between the specimen and the knife/blade during trimming
and sectioning.
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Version 1.7, Revision J
Safety 2
Warning Improper fix to software freeze. Specimen damage and/or delay of
diagnose.
· In case of a software freeze follow the instructions given in chapters
Troubleshooting and Operation.
· If instrument cannot set back into operation immediately, make sure that the
specimen is stored in an appropriate manner to avoid any damage to it.
· If required, contact Leica Biosystems service representative.
Caution After the knife/blade holder is laterally displaced, the specimen is
not retracted and retrimmed. Specimen damage.
· Retract the object head and retrim the specimen block each time after
laterally displacing the knife/blade holder.
Caution Rotating the handwheel and the coarse feed wheel at the same time.
Specimen damage.
· Do not turn the coarse feed wheel whilst rotating the handwheel.
Caution The feather key is lost when mounting the handwheel. The instrument
cannot be used, and it may cause the delay of diagnosis.
· Before assembling the handwheel, check the feather key and make sure that it
is in the handwheel shaft.
Caution Manual movement of knife/blade holder and/or object head after
setting-up the memory position. Damage of specimen.
· Do not move the knife/blade holder, the holder base and/or object head and
do not readjust the orientation without readjusting the memory position.
Caution After trimming by using the electronic coarse feed, the user does not
switch to sectioning mode. Specimen damage or unexpected functioning of the
instrument.
· After completion of trimming with the electronic coarse feed remember to
switch back to sectioning mode.
· Before starting sectioning always make sure that an appropriate sectioning
thickness has been selected.
HistoCore MULTICUT
19
2 Safety
Caution
Turning the electronic coarse feed wheel during fast homing or moving to the
memory position. Specimen impaired.
· Do not touch the electronic coarse feed wheel during fast homing or moving
to the memory position.
Caution
Turning the handwheel or pressing buttons on the separate control panel or the
instrument control panel during fast homing or moving to the memory position.
Specimen impaired.
· Do not turn the handwheel or press any button on the separate control panel
or the instrument control panel during fast homing or moving to the memory
position.
Caution
The memory position is set too close to the knife/blade. Specimen impaired.
· Make sure that the specimen does not touch the cutting edge of the
knife/blade when setting the memory position. Move the object head a little
bit backward if the cutting edge touches the specimen or is positioned very
close to the surface of the specimen when setting the memory position.
· Do not upload a specimen with different thickness using the same memory
position.
Caution
Failure to reset the memory position after the instrument is switched off or
after a power failure. Damage to specimen.
· When the instrument is switched off or there is a power failure, previously
stored memory position data is erased. Set the memory position again after
powering on the instrument.
Note
Accessories and components experience corrosion due to using
corrosive/strongly acidic/alkaline reagent or solvent with the instrument or
accessories, such as decalcified solution that contains acid, ammonium
hydroxide that contains alkali, etc. Accessories can malfunction.
· Avoid corrosive/strongly acidic/alkaline reagent or solvent dripping onto
the instrument surface or accessories.
· If such reagent or solvent has dripped onto the instrument surface or
accessories, wipe off the residue and dry the accessories sufficiently as soon
as possible.
· If such reagent or solvent is used frequently, carry out a thorough daily
cleaning of the blade holder, the universal cassette clamp (UCC), and other
accessories if necessary.
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Safety 2
2.2.4 Cleaning and maintenance
Warning Cleaning the instrument without unplugging the power plug. Electrical
shock which causes injury of persons.
· Before each cleaning, switch off the instrument and disconnect the power
plug from the mains.
Warning The liquids go into the interior of the instrument. Serious injury of
instrument/persons.
· Ensure that liquids do not enter the interior of the instrument during
operation and maintenance. Spray proper solvents or cleaners on a cleaning pad
and not directly on the instrument to prevent liquids from entering. If
liquids go into interior of instrument, contact Leica Biosystems service.
Warning Wiping the knife in the wrong direction during cleaning. Serious
injury of persons.
· Always wipe the knife from the back of the knife to the cutting edge.
Warning Removing the parts from the drying chamber (65 °C) during cleaning of
the knife/blade holder. Scalding hazard.
· Wear heat insulating gloves when removing the parts from the drying chamber
(65 °C).
Warning Replacing the fuses without switching off the instrument and
unplugging the power plug. Electrical shock which causes injury of persons.
· Turn the instrument off with the power switch and disconnect the power plug
from the mains before replacing the fuses.
Caution Using the wrong fuses which are not with the same specification
defined in the section of Technical data in the Instructions for Use. Delayed
diagnosis because the instrument does not work using wrong fuses.
· Only use fuses of the same specification defined in the section of Technical
data in the Instructions for Use.
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2 Safety
Caution
Use of improper solvents or cleaners, or sharp/hard tools to clean the
instrument or accessories. Potential instrument malfunction or delay of
diagnosis.
· Do not use any solvents containing acetone or xylene for cleaning the
instrument. · When using cleaners, please comply with the safety instructions
of the manufacturer and the
laboratory safety regulations. · Never use a sharp or hard tool to scrape the
instrument surface. · Never soak the accessories in any cleaning solvent or
water. · Clean steel knives using an alcohol-based solution or acetone. · For
cleaning and removal of paraffin, do not use xylene or cleaning fluids
containing alcohol (for
example glass cleaner).
Caution
During cleaning, the parts of blade holders are mixed up. Poor section
quality.
· Do not mix up blade holders during cleaning.
2.3 Integrated protective devices
Warning
Protective devices or safety accessories provided by manufacturer have been
removed or modified. Serious injury of persons and/or property damage
including specimen damage.
· Never remove or modify any protective devices of the instrument or any
accessories. Only service personnel qualified by Leica Biosystems may repair
the instrument and access the instrument’s internal components.
· Before working with the instrument always assure that all protective devices
and safety accessories are in place and fulfill their purpose properly.
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Version 1.7, Revision J
2.3.1 Locking the handwheel 2
Safety 2
4
5
1
3
Fig. 1
There are two ways of locking the handwheel ( “Fig. 1-1”): · Using the
handwheel brake lever ( “Fig. 1-3”) on the right side of the microtome base
plate, the
handwheel can be braked in any position. 1. To brake, rotate the handwheel
brake lever in a counterclockwise direction to position ( “Fig. 1-4”).
Warning
The handwheel brake lever is not used properly, and the handwheel cannot be
braked. Serious injury of instrument/persons, or specimen damage.
· The handwheel brake lever must be exactly in the locked position. If the
handwheel brake lever is moved beyond this point, it is possible that the
handwheel is no longer braked.
2. To unlock the handwheel, turn the handwheel brake lever ( “Fig. 1-3”) back
to its original position ( “Fig. 1-5”).
· Using the handwheel locking mechanism ( “Fig. 1-2”) on the top of the
handwheel, the handwheel can be braked in the 12 o’clock position. 1. To lock
the handwheel, press the handwheel locking mechanism ( “Fig. 1-2”) outwards
and continue to turn clockwise the handwheel slowly until it locks exactly in
the 12 o’clock position. 2. To unlock the handwheel, turn the handwheel
locking mechanism ( “Fig. 1-2”) inwards.
1 Fig. 2
In both cases (the handwheel brake lever and handwheel locking mechanism), the yellow LED ( “Fig. 2-1”) in the LOCK will be lit.
Tip
When using both brake systems at the same time, always move the handwheel
brake lever ( “Fig. 1-3”) to position ( “Fig. 1-5”) first. Otherwise, it may
be not be possible to release the handwheel locking mechanism ( “Fig. 1-2”).
HistoCore MULTICUT
23
2 Safety
2.3.2 Safety guard on the knife/blade holder
Each knife holder is equipped with a tightly mounted safety guard ( “Fig. 3-1”) ( “Fig. 4-1”). This makes it possible to cover completely the cutting edge in every knife or blade position.
2
1
2
Knife holder N
The safety guard ( “Fig. 3-1”) of the knife holder N can be easily positioned
via two handles
( “Fig. 3-2”). To cover the knife edge, push both cover strips of the safety
guard to the center.
Fig. 3
1
3
Two-in-one blade holder E for high and low
profile blades
2
The safety guard on the two-in-one blade holder
E consists of a red foldaway handle ( “Fig. 4-1”).
To cover the cutting edge, fold the safety guard
handle upwards as illustrated in ( “Fig. 4”).
Fig. 4
Tip
The clamping levers on the two-in-one blade holder E are not interchangeable.
The two clamping levers ( “Fig. 4-2”) ( “Fig. 4-3”) must remain in the
position shown at all times, as otherwise isolated malfunctions of the two-in-
one blade holder E can occur. The clamping lever for the blade ( “Fig. 4-2”)
is at the right side, the clamping lever for the lateral displacement ( “Fig.
4-3”) is at left side.
24
Version 1.7, Revision J
Instrument Components and Specifications 3
3. Instrument Components and Specifications
3.1 Overview — instrument components
1
13 2
12 3
11
4
5
Fig. 5
1 Top tray 2 Coarse feed wheel 3 Separate control panel 4 Microtome base 5
Blade holder base 6 Two-in-one blade holder E 7 Clamping lever of
the knife/blade holder base
10 9 8 7
6
8 Anti-static waste tray 9 Handwheel brake lever 10 Object head with
orientation and UCC 11 Handwheel 12 Handwheel locking mechanism 13 Instrument
control panel
HistoCore MULTICUT
25
3 Instrument Components and Specifications
Rear view
1
5
2 6
3
4
Fig. 6
7
1 Magnet 2 Voltage selector 3 Main switch 4 Power supply
5 Vents 6 Connection for service 7 Fuse housing and fuses
3.2 Instrument specifications
Basic instrument with separate control panel, programmable retraction with
ON/OFF selection, ergonomically positioned electronical coarse feed wheel with
user selectable turn directions, without specimen orientation or additional
accessories. 100/120/230/240V AC, 50/60Hz. · Semi-Motorized Rotary Microtome
with low-maintenance and backlash-free precision micrometer
feed system with step motor. · Horizontal feed and vertical stroke mechanisms
are based on cross roller bearings. · Smooth-running hand-wheel allows two
manual sectioning modes: rocking mode and conventional
manual sectioning with full hand-wheel rotation. · Two independent hand-wheel
locking systems. · Operator-adjustable force balancing system with spring
force compensation offers two advantages:
1. Flexibility to adapt the spring force to different weights of
specimen/clamps, minimizing the risk of an object head dropping into the
knife.
2. No need for a heavy counter balance in the hand-wheel. · All important
controls are located on the user friendly separate control panel with
adjustable
inclination angle for ergonomic operation. · Section thickness settings for
trimming and sectioning can be selected and saved independently of
each other. · Important operating information is indicated on the front of the
instrument:
1. trimming or section thickness, 2. specimen retraction (Retract), 3. hand-
wheel/specimen head locking function (Lock), 4. section counter and section
thickness totalizer with reset function. · Programmable specimen retraction
system with ON/OFF function. · Rocking mode function on the control panel for
rapid trimming. Allows turning the hand-wheel back and forth over a short
distance without the need of deactivating the retraction. Each change in the
26
Version 1.7, Revision J
Instrument Components and Specifications 3
sense of rotation will be electronically detected and automatically converted
into an advance or retraction movement of the specimen, without compromising
the ribboning of the sections. · Motorized horizontal specimen head movement
can be done in 2 ways:
1. Using the coarse feed buttons on the control panel in two speeds per
direction in continuous or in step feeding mode.
2. Using the ergonomically positioned coarse feed wheel which can be
personalized by user selectable preferred turn direction.
· Visual/acoustic signals indicate the remaining feed and the front and rear
travel limits. · Efficient and rapid specimen exchange
1. by using the user programmable Memo position 2. the fast homing function
of the object head within 13 +/- 2 seconds from front to rear position. ·
Large top surface area allows placement of objects that require a flat
surface. · Removable top tray allows storage of sectioning tools and prevents
items from falling.
3.3 Technical Data
General
Nominal supply voltages Nominal frequency Maximum power consumption Protection
class Power fuses Pollution degree (according to IEC-1010, UL 3101, EN 61010)
Overvoltage category (according to IEC-1010, UL 3101, EN 61010) IP protection
class Operating temperature range Operating relative humidity Operating
altitude Transport temperature range Storage temperature range Transport and
storage relative humidity
Dimensions and weights
Basic instrument Width (including the handwheel and the coarse feed wheel)
Depth (including the section waste tray) Height (without the top tray) Height
(with the top tray) Weight (without accessories) Volume of the section waste
tray Control panel
100/120/230/240 V AC 50/60 Hz 40 VA I 2 x T 1.0 AL, 250V 2
II
IP20 +18 °C to +30 °C 20 % to max. 80 % non-condensing Up to 2000 m above sea
level -29 °C to +50 °C +5 °C to +50 °C 10 % to max. 85 % non-condensing
477 mm
620 mm 295 mm 303 mm Approx. 31 kg 1400 ml
HistoCore MULTICUT
27
3 Instrument Components and Specifications
Dimensions and weights Width Depth Height Height (in inclined position) Weight
(net) Microtome Sectioning thickness setting Section thickness setting range
Setting values
Trimming thickness setting range Setting values
Horizontal feed range Vertical stroke length Maximum cutting range without
retraction Maximum sectioning area with retraction Maximum specimen block size
for large standard clamp (H x W x D) Maximum specimen block size for super
cassette clamp (H x W x D) Specimen retraction in manual sectioning mode
Electric coarse feed · slow forward and backward buttons · fast forward · fast
backwards (fast homing) Memo position Coarse feed wheel turn direction
clockwise or counter-clockwise
94 mm 193 mm 50 mm 81 mm approx. 0.5 kg
0.50 – 100 µm · From 0.5 – 5.0 µm in 0.5 µm increments · From 5.0 – 20.0 µm in
1.0 µm increments · From 20.0 – 60.0 µm in 5.0 µm increments · From 60.0 –
100.0 µm in 10.0 µm increments 1 – 600 µm · From 1.0 – 10.0 µm in 1.0 µm
increments · From 10.0 – 20.0 µm in 2.0 µm increments · From 20.0 – 50.0 µm in
5.0 µm increments · From 50.0 – 100.0 µm in 10.0 µm increments · From 100.0 –
600.0 µm in 50.0 µm increments 24 mm ±1 mm 70 ± 1 mm 65 mm without specimen
orientation 60 mm 55 x 50 x 30 mm
68 x 48 x 15 mm
5 – 100 µm in 5 µm increments; can be turned off
· 300 µm/s · 800 µm/s · 1800µm/s 1 User selectable
28
Version 1.7, Revision J
Instrument Components and Specifications 3
Optional accessories Specimen orientation with zero postion
Two-in-one blade holder · Lateral displacement function East-west movement ·
Movement of blade holder base:
· Horizontal rotation: ± 8° · Vertical rotation: ± 8°
· 3 positions · North-south: ± 24 mm
HistoCore MULTICUT
29
4 Setting up the instrument
4. Setting up the instrument
4.1 Installation site requirements
· Stable, vibration-free laboratory table with horizontal, flat table top, as
far as possible vibration-free ground.
· No other instruments nearby which might cause vibrations. · Room temperature
consistently between + 18 °C and + 30 °C. · Obstruction-free access to the
handwheel. · To ensure proper function of the instrument, it must be set up
while maintaining a minimum distance
of 10 cm from walls and furniture. · The instrument must be installed in a
place that ensures an easy disconnection from the power
supply. The power cable must be in a place that can be easily reached.
Danger
Explosive hazard. Death or Serious injury of persons, and/or property damage.
· Never operate the instrument in rooms with an explosive hazard.
4.2 Standard delivery – packing list
HistoCore MULTICUT configuration: 14 9MULTI0C1
Quantity Part description
1
HistoCore MULTICUT basic instrument
1
Fine-directional fixture for specimen clamp
1
Quick clamping system
1
Universal cassette clamp
1
Blade holder base
1
2-in-1 Blade holder E
HistoCore MULTICUT basic instrument includes the following delivery list. Quantity Part description
HistoCore MULTICUT basic instrument
1
Handwheel, assembly
1
Separate control panel, HistoCore MULTICUT
1
Anti-static waste tray
1
Top tray
1
Service pack
2
Fuse Lead T1.0AL, 250V
1
Schnorr-Safety Washer 10×6.4×0.7
1
Hexagon screw M6x16 DIN7984
1
Cover disc
Order No. 14 0518 56372 14 0502 37717 14 0502 37718 14 0502 37999 14 0502
55546 14 0502 54497
Order No. 14 0518 56372 14 0501 38181 14 0518 56384 14 0517 56237 14 0517
56261 14 0503 43948 14 6000 04804 14 3017 00073 14 2101 23130 14 3025 00008
30
Version 1.7, Revision J
Setting up the instrument 4
Quantity 1
Part description
Instructions for Use (printed English with language CD 14 0518 80200)
Order No. 14 0518 80001
It is possible to configure a basic instrument with the below listed accessories to fit your application. To have a workable configuration, at least one item of the categories mentioned below must be ordered.
Basic instrument HistoCore MULTICUT
14051856372
Basic instrument HistoCore MULTICUT without all of the following: orientation, quick clamping system, specimen clamp, blade or knife holder set-up
select one
Specimen orientation
14050237717 Fine-directional fixture for specimen clamp (*)
14050238949 Directional fixture for specimen clamp (*)
14050238160 Rigid fixture for specimen clamp
must be ordered in addition to fixture orienting with (*)
Quick clamping system () 14050237718 Quick clamping system ()
Order at least one specimen clamp
Specimen clamps
14050237999 Universal cassette clamp
14050238005 Standard specimen clamp 50×55 mm
14050237998 Standard specimen clamp 40×40 mm
14050238967 Super cassette clamp
14050246573 Leica RM CoolClamp
Order at least one blade or knife holder base and one blade or knife holder
Blade holder base and Blade holder
14050255546 14050254497
Blade holder base 2-in-1 blade holder E
Knife holder base and knife holders
14050237962 14050237993
Knife holder base Knife holder N
14050238961 Knife holder E w/trough low-profile
Additional optional accessories and knives/blades can be found in Chapter 6 (
P. 61 6. Optional Accessories).
The country specific power cord needs to be ordered separately. Please find a
list of all power cords
available for your device on our website www.LeicaBiosystems.com within the
product section.
Tip
The accessories ordered are included in a separate box.
Please check all delivered parts against the packing list and against your
order to verify whether the delivery is complete. Should you find any
discrepancies, please contact your Leica Biosystems sales office without
delay.
HistoCore MULTICUT
31
4 Setting up the instrument
4.3 Unpacking and installation
Warning
Accessories/instrument falling out of the package during unpacking. Serious
injury of persons and/or property damage.
· Be careful when unpacking the instrument. · Once unpacked, the instrument
must be moved only in an upright position. · Thoroughly follow the
instructions given in the Unpacking Instructions attached at the outside of
the package or the actions described in the Instructions for Use.
Warning
Instrument is lifted inappropriately. Serious injury of persons and/or
property damage.
· When lifting the instrument touch it only at the lifting points described in
the Instructions for Use and Unpacking Instructions (base plate at the front
and under the instrument on the back).
· Never lift the instrument by the handwheel grip, electric coarse feed wheel,
or object head. · Always remove the section waste tray before transporting the
instrument.
Warning
Careless moving of the instrument. Serious injury of hands and/or fingers due
to getting crushed between instrument and working surface.
· When moving the instrument, touch it only at the lifting points described in
the Instructions for Use and Unpacking Instructions (base plate at the front
and under the instrument on the back).
· Thoroughly pay attention that hands do not get between instrument and
working surface.
Warning
Instrument not positioned safely with all 4 feet on an appropriate laboratory
table. Serious injury of persons and/or property damage.
· Put the instrument only on a stable, vibration-free laboratory table with
horizontal, flat table top. Ground needs to be vibration-free as far as
possible.
· Always make sure that all 4 feet are positioned completely on the laboratory
table. · If the instrument has been potentially moved by other persons (for
example for servicing) always
double-check correct positioning.
Caution
Accessories/components loosening/damaged during transportation. Property
damage or delayed diagnosis.
· The packaging has the ShockDot Impact Indicator, that indicates improper
transport. When the instrument is delivered, check this first. If the
indicator is triggered, the package was not handled as prescribed. In this
case, please mark the shipping documents accordingly and check the shipment
for damage.
32
Version 1.7, Revision J
Setting up the instrument 4
Tip The transport carton and included retaining elements should be kept in
case a return shipment is necessary later. To return the instrument, follow
the instructions below in reverse order.
1
2
· Remove the packing strap and the adhesive
tape ( “Fig. 7-1”).
· Remove the carton lid ( “Fig. 7-2”).
Fig. 7 3
Fig. 8
4 44
· Take out the accessory carton (optional
accessories) ( “Fig. 8-3”) and the cartons ( “Fig. 8-4”) for the standard
scope of delivery.
HistoCore MULTICUT
33
4 Setting up the instrument
5 6 7
8 9
· Take out the fixing module ( “Fig. 9-5”). To do so, hold it by the top edge
of the module and in the recessed grip ( “Fig. 9-6”) and pull it out by
pulling upwards.
· Remove the outer carton wall ( “Fig. 9-7”).
· Lift the instrument ( “Fig. 9-8”) by holding it by the base plate at the
front ( “Fig. 10-11”) and under the instrument on the back ( “Fig. 10-12”) and
lift it out of the formed cushion ( “Fig. 9-9”). (The instrument illustration
is provided as an example only.)
Fig. 9
· Place the instrument on a stable laboratory
11
table. The two sliding elements ( “Fig. 10-10”)
12
located on the rear of the base plate make it
easier to move the instrument on the table.
· To move the instrument, hold it by the front of
the base plate ( “Fig. 10-11”), lift it up gently
10
and slide it on its slides.
Fig. 10
4.4 Assembling the handwheel
Tip The handwheel has to be assembled before attempting to use the instrument.
The necessary parts and tools can be found in the tool kit supplied in the
delivery.
34
Version 1.7, Revision J
Setting up the instrument 4
2 3 1
5 8
Fig. 11
6 7
The feather key ( “Fig. 11-2”) is loosely placed in the handwheel shaft (
“Fig. 11-1”) and fixed in place with a cable tie during transport.
1. Remove the cable tie ( “Fig. 11-3”).
Caution
The feather key is lost when mounting the handwheel. The instrument cannot be
used, and it may cause the delay of diagnosis.
· Before assembling the handwheel, check the feather key and make sure that it
is in the handwheel shaft.
2. Place the handwheel ( “Fig. 11-5”) on the handwheel shaft ( “Fig. 11-1”)
as shown. 3. Tighten the screw ( “Fig. 11-6”) located in the center hole of
the handwheel with a Hexagon
key No. 4 ( “Fig. 11-7”). 4. Remove the cover foil from the self-adhesive
cover disk ( “Fig. 11-8”) and fix the cover disk on
the handwheel.
4.5 Electrical connections
Warning
Connecting the instrument to a non-grounded socket, or use an extension cord.
Risk of electrical shock which causes injury to persons, or delayed diagnosis.
· The instrument must be connected to a grounded socket. · Do not use an
extension cord.
4.5.1 Checking the voltage
Warning
Wrong voltage of power supply selected at the voltage selector. Instrument
damage, delay of specimen processing.
· Assure the voltage is fit for your mains voltage before plugging in the
instrument. · In need of changing the voltage setting, contact Leica
Biosystems service.
HistoCore MULTICUT
35
4 Setting up the instrument
1
2
3
Fig. 12
The voltage selector ( “Fig. 12-1”) is located on the upper right of the main
switch ( “Fig. 12-2”), on the left-rear side of the instrument. Before
switching on the instrument, check if the voltage conforms to local voltage
standards. If the voltage does not conform to local standards, contact Leica
Biosystems service. 4.5.2 Connecting the power supply · Before connecting the
power cable, make sure that the main switch ( “Fig. 12-2”) on the rear of the
instrument is switched to “O” = OFF. · Make sure that the power cable used has
the correct plug for the power socket. · Insert the connector of the power
cable into the connection socket ( “Fig. 12-3”) and plug the power
plug into the power socket. 4.5.3 Connecting the control panel
3 2
Fig. 13
4
1
The connecting cable ( “Fig. 13-1”) of the control panel is fixed to the
microtome. It may not be disconnected.
1. Insert the plug ( “Fig. 13-2”) of the connecting cable into the connecting
jack ( “Fig. 13-3”) on the rear of the control panel.
2. To fasten the plug, tighten the two screws ( “Fig. 13-4”).
36
Version 1.7, Revision J
Setting up the instrument 4
4.6 Switching on the instrument
Warning
Condensation inside the instrument due to exposure to extreme differences in
temperature and high air humidity. Damage of the instrument.
· Always make sure that for both storage and operation appropriate climate
conditions are met. See the section of Technical data ( P. 27 3.3 Technical
Data).
· After transporting the instrument, wait at least two hours to allow the
instrument to adopt the ambient temperature before turning it on.
Tip
When turning the instrument on with the power switch, do not press any of the
buttons of the control panel, except when you set specific functions. For more
information, please refer to ( P. 40 Key combination).
Turn the instrument on with the power switch at the rear. I = ON; O = OFF This
is followed by a beep. The instrument initializes.
The display (here only as an example) of the software version follows in the
four-digit LED display. This display disappears after 2 seconds and “00.00” is
displayed. After the microtome is switched on, the display fields and LEDs of
all activated functions are lit up on the instrument’s control panel and
display field.
The three-digit LED display indicates the last value set for the section
thickness or trimming thickness, depending on which setting was last
activated. This is indicated simultaneously on the control panel and
microtome. The LED of the active mode (here the section thickness) is
illuminated in green.
If the yellow LED in the LOCK field of the control panel is lit, the handwheel
locking mechanism or the handwheel brake lever is activated. The instrument
cannot be used as long as the LED is lit.
HistoCore MULTICUT
37
5 Operation
5. Operation
5.1 Operating elements and their functions
The operating functions of the microtome are divided between a control panel
and a display unit on the microtome. A control panel on the instrument
displays the current operating mode as well as various settings. All operating
functions are centrally located in the separate control panel. All buttons and
displays are logically arranged in functional groups and easily identifiable.
Warning Improper fix to software freeze. Specimen damage and/or delay of
diagnose.
· In case of a software freeze follow the instructions given in chapters
Troubleshooting and Operation.
· If instrument cannot set back into operation immediately, make sure that the
specimen is stored in an appropriate manner to avoid any damage to it.
· If required, contact Leica Biosystems service representative.
5.1.1 Instrument control panel
1
5
6 2
7
8 3
9
4
10
Fig. 14
11
1 LED – RETRACT 2 Three-digit display
3 Four-digit display 4 MENU MODE button
Lights up during specimen retraction. Displays section thickness/trimming thickness and other setting indications. Displays the section counter number. Changes between section thickness sum and section counter.
38
Version 1.7, Revision J
Operation 5
5 LED – LOCK
Lights up when the handwheel lock is active.
6 Green LED – TRIM
Lights up when the trimming mode is active.
7 Green LED – SECT
Lights up when the sectioning mode is active.
8 Green LED
Section thickness sum – shows the sum of all sections.
9 Green LED
Section counter – displays the number of all sections.
10 CLEAR button
Resets section counter and section thickness sum (to 0).
11 MENU MODE + CLEAR Pressing two buttons simultaneously switches to the setting of the retraction value.
5.1.2 Separate control panel
1
8
9
2
3
10
11
4
5
12
6
7
13
Fig. 15
1 Three-digit display
2 Buttons 3 ROCK button 4 Yellow LED
5 Coarse feed button fast backward
6 Yellow LED
7 Coarse feed button fast forward
Displays section thickness/trimming thickness and other setting indications.
Sets the section thickness/trimming thickness. Enables/Disables the rocking
mode. · Flashes during coarse feed backwards;
· Lights up when the rear end position is reached. · In the
trimming/sectioning mode: coarse feed backward fast;
· In the sectioning mode (Step mode activated): multiple step backward.
· Flashes during coarse feed forwards;
· Lights up when the remaining feed area is reached. · In the
trimming/sectioning mode: coarse feed forward fast;
· In the sectioning mode (Step mode activated): multiple step forward.
HistoCore MULTICUT
39
5 Operation
8 Green LED – TRIM 9 Green LED – SECT 10 TRIM/SECT button 11 MEMO button 12
Coarse feed button –
slow backward
13 Coarse feed button slow forward
Lights up when the trimming mode is active. Lights up when the sectioning mode
is active. Switches between the sectioning mode and the trimming mode. Sets
one memory position. · In the trimming/sectioning mode: coarse feed backward
slow;
· In the sectioning mode (Step mode activated): single step backward. · In the
trimming/sectioning mode: coarse feed forward slow;
· In the trimming mode (Step mode activated): single step forward.
Key combination Key combination
Function
MENU MODE + CLEAR button Switch on instrument + Minus button Switch on
instrument + Plus button Switch on instrument + Slow backward button
Switch on instrument + Slow forward button
Set retraction value.
Deactivate STEP mode.
Activate STEP mode.
Set the coarse feed wheel direction as counter-clockwise-forward.
Set the coarse feed wheel direction as clockwise-forward.
5.1.3 Display and control elements
Three-digit display
This display is located both on the instrument control panel and on the separate control panel. If the SECT LED is lit up, the display shows the section thickness setting in µm. If the TRIM LED is lit up, the display shows the trimming thickness setting in µm.
Selecting the sectioning and trimming mode
To switch between the sectioning mode and the trimming mode, press the
TRIM/SECT button on the separate control panel. Whenever the button is
pressed, the display toggles between SECT and TRIM. In the SECT display, the
sectioning thickness in the range from 0.50 to 100.0 µm is shown, and in the
TRIM display, the trimming thickness between 1.0 and 600 µm is displayed.
40
Version 1.7, Revision J
Operation 5
Setting the section thickness/trimming thickness
Adjust the settings using these two buttons on the separate control panel.
Section thickness setting range: 0.50 µm – 100 µm Setting values: from 0.5 µm
– 5.0 µm in 0.5 µm increments from 5.0 µm – 20.0 µm in 1.0 µm increments from
20.0 µm – 60.0 µm in 5.0 µm increments from 60.0 µm -100.0 µm in 10.0 µm
increments Trimming thickness setting range: 1 – 600 µm Setting values: from
1.0 µm – 10.0 µm in 1 .0 µm increments from 10.0 µm – 20.0 µm in 2.0 µm
increments from 20.0 µm – 50.0 µm in 5.0 µm increments from 50.0 µm -100.0 µm
in 10.0 µm increments from 100.0 µm – 600.0 µm in 50.0 µm increments
Coarse feed functions
1 Fig. 16
The electric coarse feed at two speeds is used for a rapid movement of the specimen towards and away from the knife. With the double-arrow buttons, the coarse feed operates at 800 µm/s when moving forwards; with the single-arrow buttons, it runs at 300 µm/s when moving forwards and backwards. In the sectioning mode the coarse drive can be used in STEP mode, or with continuous feed. The instrument is delivered with continuous feed (standard configuration). Fast homing Press the fast-backward coarse feed button ( “Fig. 16-1”), the object head is moving from the front end position to the home position at 1800µm/s.
Caution
Turning the electronic coarse feed wheel during fast homing or moving to the
memory position. Specimen impaired.
· Do not touch the electronic coarse feed wheel during fast homing or moving
to the memory position.
Caution
Turning the handwheel or pressing buttons on the separate control panel or the
instrument control panel during fast homing or moving to the memory position.
Specimen impaired.
· Do not turn the handwheel or press any button on the separate control panel
or the instrument control panel during fast homing or moving to the memory
position.
HistoCore MULTICUT
41
5 Operation
Sectioning mode
1
2
3
4
Fig. 17
Trimming mode
12
3
4 Fig. 18
In the sectioning mode the user can select between STEP function (step-bystep
specimen feed) and continuous specimen feed. When the continuous feed is
selected, the coarse feed buttons have the same functions as in the trimming
mode. The STEP function is useful for careful step-by-step approximation of
the specimen towards the knife. How to activate the STEP function:
· Switch the instrument on while holding the + button on the control panel.
(Likewise, to deactivate switch the instrument on while pressing the button.)
While the instrument is initializing, hold the + button until the software
version number is no longer displayed.
· Press the TRIM/SECT button and select the sectioning mode (LED SECT lit).
· When pressing the slow coarse feed button ( “Fig. 17-2”) or ( “Fig. 17-4”)
for slow-speed coarse feed, a defined, incremental feed takes place (STEP) at
the value indicated on the display in the respective direction (single step).
· By short activation of the fast coarse feed buttons, a single step is also
effected in the appropriate direction.
· Longer activation of the fast coarse feed button ( “Fig. 17-1”) or ( “Fig.
17-3”) effects a repeated feed motion for as long as the button is pressed.
In the trimming mode, the coarse feed buttons operate a continuous movement as
long as the button is held down. The double-arrow button for fast coarse feed
backward movements has a lock-in function. Do not put your fingers between the
specimen clamp and microtome to prevent pinching them.
· To start the fast backwards movement (away from the knife) press the button
( “Fig. 18-1”). After the button is pressed, the specimen head is moved to the
rear end position.
· To stop the movement, press any of the four coarse feed buttons.
· The yellow LED ( “Fig. 18-2”) in the button flashes while the specimen head
is in motion, and remains lit continuously when the rear end position is
reached.
· Press the button ( “Fig. 18-3”) to start the slow backwards movement. The
travel continues as long as the button is held pressed.
· Press the appropriate button to start a rapid or slow forward movement. The
travel continues as long as the button is held pressed.
· During the forwards movement, the yellow LED ( “Fig. 18-4”) in the button
flashes. When the front end position is reached, an acoustic signal is heard
and the LED stops flashing and remains lit.
42
Version 1.7, Revision J
Operation 5
One memory position
1 2 3
4 5 Fig. 19
Set one memory position using the MEMO button ( “Fig. 19-1”) on the separate control panel. To set one memory position, move the object head to the desired position, and press the MEMO button. A short beep is heard; the three-digit display indicates SEt ( “Fig. 19-2”); the LED ( “Fig. 19-3”) of the MEMO button lights up in green. If one memory position is stored, after pressing the MEMO button, the object head moves to the memory position; a short beep is heard and the three-digit display indicates At ( “Fig. 19-4”) when the object head reaches the memory position. To change the stored memory position, move the object head to the new position, and press on the MEMO button for about 1 second. A short beep is heard; the three-digit display indicates SEt; the green LED of the MEMO button keeps lighting on. To clear the memory position, press on the MEMO button for more than 3 second. Two short beeps are heard; the three-digit display indicates CLr ( “Fig. 19-5”); the green LED of the MEMO button is greyed out.
Tip
While the object head is moving to the memory position, the movement can be
stopped by pressing the MEMO button or any other buttons, or by rotating the
electronic coarse feed wheel. The MEMO button is enabled between the front
limit position (not included) and home position (not included).
Caution
Manual movement of knife/blade holder and/or object head after setting-up the
memory position. Damage of specimen.
· Do not move the knife/blade holder, the holder base and/or object head and
do not readjust the orientation without readjusting the memory position.
Caution
Turning the electronic coarse feed wheel during fast homing or moving to the
memory position. Specimen impaired.
· Do not touch the electronic coarse feed wheel during fast homing or moving
to the memory position.
Caution
Turning the handwheel or pressing buttons on the separate control panel or the
instrument control panel during fast homing or moving to the memory position.
Specimen impaired.
· Do not turn the handwheel or press any button on the separate control panel
or the instrument control panel during fast homing or moving to the memory
position.
HistoCore MULTICUT
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5 Operation
Caution
The memory position is set too close to the knife/blade.
Specimen impaired. · Make sure that the specimen does not touch the cutting
edge of the knife/blade when setting the memory position. Move the object head
a little bit backward if the cutting edge touches the specimen or is
positioned very close to the surface of the specimen when setting the memory
position. · Do not upload a specimen with different thickness using the same
memory position.
Caution
Failure to reset the memory position after the instrument is switched off or
after a power failure. Damage to specimen.
· When the instrument is switched off or there is a power failure, previously
stored memory position data is erased. Set the memory position again after
powering on the instrument.
Four-digit display on the instrument control panel
The value showing on thefour-digit display is adjustable. 1 When the -µm LED (
“Fig. 20-1”) is lit, the display shows the sum of the
section thicknesses in µm for all sections completed since the instrument
was switched on. When the -n LED ( “Fig. 20-2”) is lit, the display shows the
number of all 2 previously completed sections.
3 Fig. 20
· To change the display mode, push MENU MODE button ( “Fig. 20-3”) until
4
the LED of the desired mode is illuminated.
· Press CLEAR button ( “Fig. 20-4”) to reset section thickness sum or section number.
· This will only reset the currently displayed value.
Tip
When the instrument is switched off using the main power switch, both values
(section thickness sum and section number) are erased from memory.
Specimen retraction
To prevent damage to the knife and specimen, the specimen is moved away from
the knife during the return motion to the upper home position of the object
head. The retraction value can be selected in 5 µm increments between 5 and
100 µm. Specimen retraction is set to 10 µm at the factory. Specimen
retraction can be disabled if required. The selected setting is maintained
when the instrument is turned off.
44
Version 1.7, Revision J
Operation 5
Configuring the retraction settings
1 3 4
6
· To call up the retraction settings, press the MENU MODE ( “Fig. 21-1”) and
CLEAR ( “Fig. 21-2”) buttons simultaneously.
2 · The current set value is displayed as a three-digit number in the four-
digit display, e.g. 025 = 25 µm ( “Fig. 21-3”).
· Select the desired retraction value. The retraction value can be adjusted in
increments of 5 µm to a maximum of 100 µm using buttons ( “Fig. 21-4”) or
5 ( “Fig. 21-5”) on the separate control panel. The setting can be off too.
· To exit the retraction settings, press MENU MODE ( “Fig. 21-6”). A
retraction movement takes place by the newly selected value after each
section.
7 Fig. 21
· While the specimen is in retraction, the yellow LED ( “Fig. 21-7”) on the RETRACT display lights up.
1 2
3 4 Fig. 22
· To switch off retraction, press the button ( “Fig. 22-1”) on the separate
control panel until the display indicates OFF ( “Fig. 22-2”).
· To exit the retraction settings, press MENU MODE ( “Fig. 22-3”). When
retraction is switched off, the specimen is not retracted. The yellow LED (
“Fig. 22-4”) of the RETRACT display does not light up.
Indication of remaining horizontal feed
4 Fig. 23
The visible and audible remaining feed indication feature informs
2 the user during trimming and sectioning when a remaining feed of
1
approximately 1 mm ( “Fig. 23-3”) is available before the front limit
is reached.
The yellow LED ( “Fig. 23-2”) in the coarse feed button lights up
from the beginning of the remaining feed.
In addition, an acoustic signal is heard for approx. 2 seconds.
3
From this point on, a remaining feed of approx. 1 mm is available.
In the remaining feed area, no more object feeding to the knife is
5
possible using the coarse feed buttons and the electronic coarse
feed wheel.
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45
5 Operation
Fig. 24
· You can continue with your working routine.
· The yellow LED ( “Fig. 23-2”) in the coarse feed button lights up.
· When the front end position ( “Fig. 23-5”) is reached, no more infeed takes
place; this also means that no sectioning takes place.
· You can continue to work on the specimen by pressing the corresponding
coarse feed button ( “Fig. 24”) in the rear end position ( “Fig. 23-4”) and
continuing with sectioning.
Tip
To continue your work, you must press TRIM/SECT button to switch to trimming
mode, as otherwise, you cannot use the coarse feed. If the specimen head is
already in the remaining feed range when the instrument is switched on, an
additional acoustic signal is heard after the software version is displayed.
· You can continue to work on the specimen by moving it back a short distance using the coarse feed buttons (set trimming mode.).
· The STEP function is disabled in the remaining feed range.
5.1.4 Electronic coarse feed wheel 1
The coarse motion serves for a fast horizontal forwards movement of the specimen – towards the knife – and backwards – away from the knife. The rotation of the electronic coarse feed wheel ( “Fig. 25-1”) can be set as clockwise or counterclockwise to advance the object head.
Fig. 25 1
3 Fig. 26
· To set clockwise rotation to advance the object
head, switch the instrument on while holding
the slow-forward coarse feed button ( “Fig.
26-1”) on the separate control panel; three-digit
display indicates C for clockwise ( “Fig. 26-2”). 2
· To set counterclockwise rotation to advance
the object head, switch on the instrument and
press the slow-backward coarse feed button
4
( “Fig. 26-3”); three-digit display indicates CC
for counterclockwise ( “Fig. 26-4”).
The settings of the electronic coarse feed wheel
rotation is shown for about 4 seconds on the
three-digit display when the instrument switches
on. The factory-set rotation direction of the
electronic coarse feed wheel is clockwise.
46
Version 1.7, Revision J
Operation 5
5.1.5 Fine directional specimen holder fixture Tip In the quick clamping
system of the directional specimen holder fixture, all specimen clamps
available as optional accessories can be used.
2
4
4
3 1
Fig. 27
The specimen orientation allows for simple position correction of the specimen
surface when the specimen is clamped into place. The directional specimen
holder fixture may be exchanged for a non-directional fixture (optional
accessory).
Display of the zero position
For better display of the zero position, the orientation has two red
indicators ( “Fig. 27-4”). When both indicators are fully visible and both
setscrews are in zero position at the same time (white markings align with the
arrows), the specimen is in zero position.
Orienting the specimen
Warning
Specimen is oriented in retraction phase. Specimen damage due to re-orienting
the specimen in retraction phase.
· Specimen blocks must not be oriented during the retraction phase. If a block
is oriented during retraction, the block will advance by the retraction value
plus the selected section thickness before the next section. This may cause
damage to both specimen and knife/blade.
1. Raise the object head to the upper end position and activate the handwheel
locking mechanism.
2. To release the clamp, turn the eccentric lever ( “Fig. 27-1”) clockwise.
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5 Operation
3. Turn setscrew ( “Fig. 27-2”) to orient the specimen in vertical direction.
Turn setscrew ( “Fig. 27-3”) to orient the specimen in horizontal direction.
Each complete turn of the screw inclines the specimen by 2°. A total of 4
complete turns = 8° are possible in every direction. The accuracy is
approximately ± 0.5°. For ease of estimation, there is a white mark on the
handle and a click stop that is noticeable during turning.
4. To lock the current orientation, turn the eccentric lever ( “Fig. 27-1”)
counterclockwise.
Tip
When the large standard specimen clamp (50 x 55 mm) or the super cassette
clamp is used, the specimen orientation of ± 8° in north-south direction is no
longer possible. The usable angle for the large standard specimen clamp (50 x
55 mm) is only about ± 4° in this case.
5.1.6 Fine adjustment of the force balance 1
Fig. 28
If another accessory of a different weight is mounted on the object head (
“Fig. 28-1”), you must check whether it is necessary to readjust the force
balance.
· Attach the new accessory and clamp the specimen.
· Set the object head to half the height of the vertical travel range by
turning the handwheel ( “Fig. 28”).
If the object head remains in this exact position, the setting is correct. If
the object head moves, i.e. it is raised or lowered, fine adjustment is
necessary.
Warning
Wrong fine adjustment of the force balance.
Serious injury of the operator due to contact with knife and/or specimen
damage. · Before working with the instrument, always double-check that the
fine adjustment of the force balance is properly set. · If fine adjustment is
not properly set, do not work with the instrument but perform a readjustment.
· Especially after changing accessories at the object head, perform a fine
adjustment of the force balance immediately.
48
Version 1.7, Revision J
Operation 5
The force balance is adjusted using the screw ( “Fig. 29-1”), which can be accessed by removing the section waste tray on the bottom of the base plate of the microtome. Use the Hexagon key No. 5 (with handle) for the adjustment.
· If the object head moves downwards, turn the
screw ( “Fig. 29-1”) approx. 1/2 turn clockwise each time.
· If the object head moves upwards, turn
the screw ( “Fig. 29-1”) approx. 1/2 turn counterclockwise each time.
1
· Continue this procedure until the object head no
longer moves once released.
Fig. 29
Tip Repeat the adjusting procedure several times till the object head no
longer moves. 5.2 Inserting the two-in-one blade holder E 5.2.1 Setting up the
blade holder base
5 1
4
3
2
Fig. 30
1. Release the clamping lever ( “Fig. 30-1”) by rotating it counterclockwise.
2. Insert the blade holder base ( “Fig. 30-2”) using the notch ( “Fig. 30-3”)
on the bottom into
the T-piece ( “Fig. 30-5”) of the microtome base plate ( “Fig. 30-4”).
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5 Operation
3. The blade holder base ( “Fig. 30-2”) can be moved back and forth on the
microtome base plate. This allows bringing the two-in-one blade holder E to
optimal sectioning position in relation to the specimen. To secure the blade
holder base, turn the clamping lever ( “Fig. 30-1”) clockwise.
5.2.2 Inserting the two-in-one blade holder E
8 7
10
Fig. 31
9
1. Loosen the eccentric bolt ( “Fig. 31-9”) using a Hexagon key No. 4 ( “Fig.
31-10”). 2. Place the two-in-one blade holder E ( “Fig. 31-8”) with the
underside groove onto the T-piece
( “Fig. 31-7”) of the knife holder base ( “Fig. 30-2”). 3. To clamp, retighten
the eccentric bolt ( “Fig. 31-9”).
5.3 Adjusting the clearance angle
2 4
1
5
6
3 Fig. 32
The index marks (0°, 5° and 10°) for adjustment of the clearance angle ( “Fig.
32-4”) are located on the right side of the two-in-one blade holder E ( “Fig.
32-2”). There is also an index mark ( “Fig. 32-5”) on the right side of the
blade holder base ( “Fig. 32-1”) which serves as a reference point when
adjusting the clearance angle.
1. Loosen the screw ( “Fig. 32-3”) using a Hexagon key No. 4 ( “Fig. 32-6”)
until the two-in-one blade holder E ( “Fig. 32-2”) can be moved.
50
Version 1.7, Revision J
Operation 5
2. Move the two-in-one blade holder E until the index mark of the desired
clearance angle coincides with the reference line on the blade holder base.
Enlarged detail ( “Fig. 32”) shows a clearance angle setting of 5°. Tips The
recommended clearance angle setting for the two-in-one blade holder E is
approx. 2.5° – 5°.
3. Hold down the two-in-one blade holder E in this position and retighten the
screw ( “Fig. 32-3”) for clamping.
5.4 Inserting the universal cassette clamp
1 1
2
3
4
7
6
Fig. 33
5
There are two specimen holder fixtures with orientation and one without,
please see ( P. 61 6. Optional Accessories). The specimen orientation allows
for simple position correction of the specimen surface when the specimen is
clamped into place. You can use the quick clamping system ( “Fig. 33-5”) to
hold all available accessory specimen clamps (for more information, see ( P.
61 6. Optional Accessories)). To do so, proceed as follows:
1. Move the object head ( “Fig. 33-1”) to the upper end position by turning
the handwheel ( “Fig. 33-6”) and engage the handwheel locking mechanism.
2. To release the clamping system, turn the screw ( “Fig. 33-2”) of the quick
clamping system ( “Fig. 33-5”) counterclockwise using a Hexagon key No. 4 (
“Fig. 33-7”).
3. Push the guide ( “Fig. 33-4”) of the universal cassette clamp ( “Fig.
33-3”) from the left into the quick clamping system ( “Fig. 33-5”) as far as
it will go.
4. To clamp the cassette clamp turn the screw ( “Fig. 33-2”) clockwise as far
as it will go.
Tip
Since all specimen clamps available as accessories are equipped with the same
kind of guide on the back, they are inserted in the same way described here
using the example of the cassette clamp.
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5 Operation
5.5 Clamping the specimen
Warning
The operator grabs into the knife or blade due to inappropriate working
procedure. Serious injury of persons when putting the specimen in place if
knife or blade has been installed before.
· Prior to uploading the specimen on the microtome, make sure that the cutting
edge has been covered by the safety guard and the hand wheel locking mechanism
has been activated. Once the operator wants to upload the specimen as well as
inserting a knife/blade, always upload the specimen block before mounting and
clamping the knife/blade.
1. Rotate the handwheel until the specimen clamp is in the upper end
position. 2. Lock the handwheel or activate the handwheel locking mechanism (
P. 23 2.3.1 Locking
the handwheel). 3. Insert a specimen into the specimen clamp.
Tip
A detailed description for inserting the specimen into various specimen clamps
and specimen holders is provided in ( P. 61 6. Optional Accessories).
5.6 Clamping the knife/disposable blade
Warning
Knives or blades are handled and/or disposed in an inappropriate manner.
Serious injury of persons due to contact with extremely sharp knives and/or
blades.
· Work especially thoroughly and carefully when handling knives and/or blades.
· Always wear dedicated clothes (including cut-resistant gloves) when handling
knives and/or
blades. · Dispose knives and blades always in a safe place (for example,
dedicated knife case) and an
appropriate manner that ensures that people cannot get hurt. · Never place a
knife anywhere with the cutting edge facing upwards and never try to catch a
falling knife. · Always cover the edge of the knife/blade with the safety
guard before clamping the specimen.
Warning
The operator installs two knives and/or blades on the knife and/or blade
holder. Serious injury of persons due to contact with extremely sharp knives
and/or blades.
· Do not clamp two knives/blades on the knife holder/blade holder. Install the
knife/blade in the center of the knife/blade holder. Knives/Blades must not
exceed the edge of the knife/blade holder.
52
Version 1.7, Revision J
Operation 5
Warning
Too thick or thin blade used in the two-in-one blade holder. Sample impaired.
· Use a blade with a maximum thickness of less than 0.322mm and a minimum
thickness of more than 0.246mm.
· The recommended compatible blade is listed in Optional Accessories ( P. 61
6. Optional Accessories).
Warning
The blade is not installed completely parallel to the upper edge of the
pressure plate due to excess paraffin, uncleaned inserts, etc.. When using the
lateral movement function, failure to install the blade parallel to the
pressure plate may result in poor sectioning results. For example, if a
section is too thick or thin, chattering within the section, in the worst-case
scenario, may damage the specimen.
· Do not continue the sectioning when getting unsatisfying section results. ·
Re-install the blade and make sure that it is parallel to the upper edge of
the pressure plate. · Always check the parallelism between the blade and
pressure plate after moving the blade using
the lateral movement function. · Ensure there is no paraffin waste and the
insert is clean before use.
Inserting high-profile blades
1
2
4 2
3
Fig. 34
Fig. 35
1. Bring the safety guard ( “Fig. 34-1”) down. 2. To insert the blade, rotate
the right clamping lever ( “Fig. 34-2”) counterclockwise. 3. Carefully push in
the blade from above or from the side. Make sure that the blade is positioned
in the center and, most importantly, parallel to the upper edge of the
pressure plate( “Fig. 35-4”).
4. To clamp the blade, rotate the clamping lever ( “Fig. 34-2”) clockwise.
Inserting low-profile blades
1
Fig. 36
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53
5 Operation
1
Fig. 37
When using low-profile blades, the insert ( “Fig. 37-1”) for low-profile blades must first be placed in the two-in-one blade holder E and make sure that the bottom edge of the insert fits closely to the groove of the blade holder. Two magnets are attached to the rear side of the insert ( “Fig. 36-1”). They point away from the operator (towards the rear pressure plate) after the insert has been inserted with the rounded edges showing upwards. Make sure that the insert is pushed all the way down and allows the blade to sit parallel to the cutting edge – if this is not done properly there is risk of damaging the specimen. Then insert the blade as described (for high profile blade) ( P. 53 Inserting high-profile blades). Removing the blades
1
3
2
1
4
2
Fig. 38
Fig. 39
1. Rotate the clamping lever ( “Fig. 38-2”) counterclockwise. 2. Push the pin
( “Fig. 38-3”) on the blade ejector .
Tips
Use the blade ejector to safely eject the blade.
3. Bring the safety guard ( “Fig. 39-1”) down. Use the brush with magnet (
“Fig. 39-4”) to remove the blade from the right side and out.
Once the blade has been removed from the two-in-one blade holder E, it is
disposed in the used blade container on the downside of the blade dispenser (
“Fig. 40”).
54
Version 1.7, Revision J
Operation 5
Fig. 40
Warning Knives or blades are handled and/or disposed in an inappropriate
manner. Serious injury of persons due to contact with extremely sharp knives
and/or blades.
· Work especially thoroughly and carefully when handling knives and/or blades.
· Always wear dedicated clothes (including cut-resistant gloves) when handling
knives and/or
blades. · Dispose knives and blades always in a safe place (for example,
dedicated knife case) and an
appropriate manner that ensures that people cannot get hurt. · Never place a
knife anywhere with the cutting edge facing upwards and never try to catch a
falling knife. · Always cover the edge of the knife/blade with the safety
guard before clamping the specimen.
Tip Detailed descriptions for inserting the knife into the individual knife
holders are provided in ( P. 61 6. Optional Accessories).
5.7 Trimming the specimen
Warning Wheel turn direction of electric coarse feed incorrectly selected.
Specimen damage.
· Always assure that you have selected the correct turn direction of the
coarse feed wheel before turning the wheel.
Caution Rotating the handwheel and the coarse feed wheel at the same time.
Specimen damage.
· Do not turn the coarse feed wheel whilst rotating the handwheel.
HistoCore MULTICUT
55
5 Operation
Fig. 41
· Use the TRIM/SECT button to select the TRIM mode.
· Set the desired trimming thickness.
· Release the handwheel locking mechanisim and the handwheel brake lever.
· In TRIM mode, use the coarse feed buttons or electronic coarse feed wheel to move the specimen towards the knife/blade.
· Trim the specimen by turning the handwheel or the coarse feed wheel
1
or
· Press the ROCK button (LED ( “Fig. 41-1”) in button is lit) to select ROCK mode. Trim the specimen by rocking the handwheel back and forth.
· Terminate trimming when the desired sectioning surface and depth have been reached.
Warning
When doing fast manual trimming and fingers are put between the specimen and
the knife/blade after the handwheel is released. Operator can get cut and
injured due to the turning of the handwheel when it is unlocked.
· Do not put fingers between the specimen and the knife/blade during trimming
and sectioning.
5.8 Sectioning
Warning
Personnel with insufficient qualification working with the instrument. Serious
injury of persons and or specimen damage can occur when specimen approaches
knife/ blade due to inappropriate operator actions, for example, because
object head may drop onto knife holder when handwheel is unlocked.
· Always make sure that only laboratory personnel with dedicated and
sufficient training and qualification operates the instrument.
· Always make sure that all laboratory personnel designated to operate this
instrument has read these Instructions for Use carefully and is familiar with
all technical features of the instrument before start working with it.
Warning
Personal protective equipment is not used. Injury of persons.
· When working with microtomes, personal safety precautions must always be
taken. It is mandatory to wear work safety shoes, safety gloves, a mask and
safety goggles.
56
Version 1.7, Revision J
Operation 5
Warning Working with the instrument and brittle specimen without appropriate
protective clothes. Serious injury of persons by splinters while cutting
brittle specimen.
· Always wear appropriate protective clothes (including safety goggles) and
work especially carefully when cutting brittle specimen.
Warning Insufficient frame conditions for sectioning. Specimen damage or poor
sectioning results,for example, sections alternating in thickness, compressed,
folded or stripped sections.
· Do not continue sectioning if you recognize insufficient sections results. ·
Make sure that all prerequisites for appropriate sectioning are met. Refer to
the troubleshooting
section of these Instructions for Use for further details. · If you do not
have sufficient knowledge in regards of troubleshooting insufficient
sectioning
results consult persons who do have that knowledge, for example, application
experts of Leica Biosystems.
Warning Turning the handwheel counterclockwise. People injury/Damage to
specimen.
· Do not turn the handwheel counterclockwise, which may lead to handwheel
locking mechanism malfunction.
Warning The rotation speed of the handwheel does not suit the hardness of the
specimen. Instrument damage and possibly specimen damage.
· The rotation speed of the handwheel must be adapted to suit the hardness of
the specimen. For harder specimens, use a slower speed.
Warning In the manual sectioning mode, the user cuts the specimen block by
rotating the handwheel at a very high speed. It may lead to poor sectioning
quality and even damage the specimen.
· In the manual sectioning mode, the rotation speed of the handwheel shall be
no more than 60 rpm.
Caution After the knife/blade holder is laterally displaced, the specimen is
not retracted and retrimmed. Specimen damage.
· Retract the object head and retrim the specimen block each time after
laterally displacing the knife/blade holder.
HistoCore MULTICUT
57
5 Operation
Caution
After trimming by using the electronic coarse feed, the user does not switch
to sectioning mode. Specimen damage or unexpected functioning of the
instrument.
· After completion of trimming with the electronic coarse feed remember to
switch back to sectioning mode.
· Before starting sectioning always make sure that an appropriate sectioning
thickness has been selected.
Note
Accessories and components experience corrosion due to using
corrosive/strongly acidic/alkaline reagent or solvent with the instrument or
accessories, such as decalcified solution that contains acid, ammonium
hydroxide that contains alkali, etc.
Accessories can malfunction.
· Avoid corrosive/strongly acidic/alkaline reagent or solvent dripping onto
the instrument surface or accessories.
· If such reagent or solvent has dripped onto the instrument surface or
accessories, wipe off the residue and dry the accessories sufficiently as soon
as possible.
· If such reagent or solvent is used frequently, carry out a thorough daily
cleaning of the blade holder, the universal cassette clamp (UCC), and other
accessories if necessary.
Fig. 42
Always use a different area of the cutting edge for trimming and sectioning. ·
To do so, laterally displace the knife/blade holder. When using the two-in-
one blade holder E with lateral displacement, it is sufficient to move the
blade holder sideways. · Use the TRIM/SECT button to select the SECT mode. ·
Adjust the appropriate sectioning thickness or verify the selected value. ·
Select conventional sectioning. · To section the specimen, turn the handwheel
clockwise smoothly. · Pick up the sections.
5.9 Changing the specimen or interrupting sectioning
Warning The handwheel is unlocked and the object head drops into knife/blade
holder. People injury/Damage to specimen.
· Except sectioning phase, the handwheel must be locked at all times.
58
Version 1.7, Revision J
Operation 5
Warning Specimen is oriented in retraction phase. Specimen and knife/blade
damage due to re-orienting the specimen in retraction phase.
· Specimen blocks must not be oriented during the retraction phase. If a block
is oriented during retraction, the block will advance by the retraction value
plus the selected section thickness before the next section. This may cause
damage to both specimen and knife/blade.
Warning Knife/blade is not covered with the appropriate guard when not
sectioning specimen. Serious injury of persons.
· Always cover the knife/blade edge with the safety guard prior to any
manipulation of knife/blade or specimen clamp, prior to changing the specimen
and during all work breaks.
Warning Uploading or unloading the specimen on the microtome without
appropriate clothes and in an inappropriate manner. Operator can get cut and
injured which can lead to serious consequences.
· Always use cut resistant safety gloves when handling the specimen within the
microtome. · Lock the handwheel and cover the knife edge with the safety guard
prior to any manipulation of
the specimen clamp and prior to changing the specimen.
1. Raise the specimen to the upper end position and activate the handwheel
locking mechanism. 2. Cover the sectioning edge with the safety guard. 3.
Remove the specimen from the specimen clamp and mount a new sample to
continue. 4. Before cutting into a new specimen, move the specimen head back
to the rear end position.
Fig. 43
5.10 Finishing the daily routine
Warning Section waste tray falling down after it has been detached. Injury of
persons.
· Work especially carefully when detaching the section waste tray and put it
in a safe place.
Warning Knife/blade was not removed when knife/blade holder was uninstalled
from the microtome. Serious injury of persons due to contact with extremely
sharp knife/blade.
· Before uninstalling the knife/blade holder from the microtome always make
sure to detach the knife/ blade wearing cut-resistant gloves and store the
knife/blade in a safe place.
HistoCore MULTICUT
59
5 Operation
Warning
Knife/blade stored in inappropriate manner. Serious injury of persons, for
example, due to unexpected fall off.
· Always store knife/blade in an appropriate place when not using them, for
example, store it in the dedicated knife case.
· Never place a knife anywhere with the cutting edge facing upwards and never
try to catch a falling knife.
Warning
Paraffin waste falls on the floor and is not cleaned-up. Serious injury of
persons, for example, by slipping and stumbling into the knife/blade.
· Always clean up paraffin waste before it spreads, becomes slippery, and
becomes a hazard. · Wear appropriate shoes.
1. Move the specimen to the upper end position by turning the handwheel and
engage the handwheel locking mechanism.
2. Remove the blade from the two-in-one blade holder E and insert it in the
receptacle at the bottom of the dispenser, or remove the knife from the knife
holder and put it back in the knife case.
3. Remove the specimen from the specimen clamp. 4. Move the object head to
the rear end position or move the knife holder on the knife holder base
outwards. 5. Push all section debris into the section waste tray and empty the
tray. 6. Switch the instrument off at the main power switch. 7. Clean the
instrument ( P. 95 8.1 Cleaning the instrument).
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6. Optional Accessories
6.1 Assembly for fixture for specimen clamps
Tips Depending upon the purchase order, the basic instrument is delivered with
the fine directional, directional or rigid fixture for specimen clamps which
must be assembled first. All specimen clamps available as accessories can be
used in one of the three fixtures for specimen clamps. Before assembling the
fixture for specimen clamps, activate the handwheel locking mechanism.
6.1.1 Rigid fixture for specimen clamps Tips Remove the rubber ring ( “Fig. 44-5”) only after attaching the object head.
2
4
3
5
1 Fig. 44
Screw the rigid fixture for specimen clamps ( “Fig. 44-4”) onto the object
head ( “Fig. 44-3”).
· Remove the screw ( “Fig. 44-1”), place the specimen holder fixture ( “Fig.
44-4”) onto the object head ( “Fig. 44-3”) from the front and tighten the
screws ( “Fig. 44-2”) with a Hexagon key No. 3.
· Next, insert the screw ( “Fig. 44-1”) from the side and briefly tighten it
with a Hexagon key No. 4.
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6.1.2 Directional fixture for specimen clamps
1 2
3 4
Fig. 45
7
5
6 8
6.1.3 Fine-directional fixture for specimen clamps
· Loosen the eccentric bolt ( “Fig. 45-1”) by turning it counterclockwise.
· Completely unscrew the thrust piece ( “Fig. 45-2”) with a flat-tip
screwdriver and pull it out with spring ( “Fig. 45-3”) and pin ( “Fig. 45-4”).
· Completely unscrew the setscrews ( “Fig. 45-5”) and ( “Fig. 45-6”).
· Install the directional fixture for specimen clamps by tightening the screws
in the bores ( “Fig. 45-8”) (2 screws are accessible through the bores) and
screw them in using a Hexagon key No. 3. Insert the screws ( “Fig. 45-7”) in
the bores as shown and screw them in using a Hexagon key No. 3.
· Insert the spring ( “Fig. 45-3”) and pin ( “Fig. 45-4”) with the flatter
side into the thrust piece ( “Fig. 45-2”). Completely screw in the thrust
piece with a flat-tip screwdriver.
· Completely screw in the setscrews ( “Fig. 45-5”) ( “Fig. 45-6”).
1
2
4
3
· Before the fine-directional fixture for specimen clamps can be mounted,
loosen 4 screws ( “Fig. 46-2”) (Hexagon key No. 3) and carefully remove the
fixture for specimen clamps from the baseplate ( “Fig. 46-1”).
· Using the 4 supplied screws ( “Fig. 46-3”) and the Hexagon key No. 3, fasten
the baseplate to the object head ( “Fig. 46-4”).
· Now, screw the fine-directional fixture for specimen clamps with the 4
screws ( “Fig. 46-2”)and the Hexagon key No. 3 onto the object head.
Fig. 46
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Optional Accessories 6
Tips If the fine-directional fixture for specimen clamps is not used, retain
the baseplate ( “Fig. 46-1”) and 4 screws ( “Fig. 46-3”) together with the
fine-directional fixture for specimen clamps.
6.1.4 Quick clamping system
It is used as an adaptor for use with the fine-
directional fixture for specimen clamps with
zero point indicators or the directional fixture for
specimen clamps.
Screw the 4 screws ( “Fig. 47-2”) into bore
1
( “Fig. 47-1”) with a Hexagon key size 2.5 and
tighten them.
2
Fig. 47
1
6.2 Specimen clamps and holders
Tips
All specimen clamps available as accessories can be integrated into the fine
directional, or directional, or non-directional specimen holder fixtures. For
how to install the specimen clamps and holders to the quick clamping system,
please refer to ( P. 51 5.4 Inserting the universal cassette clamp).
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6 Optional Accessories
6.2.1 Standard specimen clamp 1
2 3
Fig. 48
The standard specimen clamp is available in two sizes: 40 x 40 mm and 50 x 55
mm. They are designed for direct clamping of rectangular blocks. In addition,
they accommodate the foil clamp and V-insert.
· Turn the knurled screw ( “Fig. 48-1”) counterclockwise to move the movable
jaw ( “Fig. 48-3”) downward.
· Mount the specimen ( “Fig. 48-2”) as required.
· Turn the knurled screw ( “Fig. 48-1”) clockwise to move the movable jaw
upward against the fixed jaw to securely clamp the sample.
Warning
Insufficient frame conditions for sectioning.
Specimen damage or poor sectioning results, for example, sections alternating
in thickness, compressed, folded or stripped sections.
· Do not continue sectioning if you recognize insufficient sections results. ·
Make sure that all prerequisites for appropriate sectioning are met. Refer to
the troubleshooting
section of these Instructions for Use for further details. · If you do not
have sufficient knowledge in regards of troubleshooting insufficient
sectioning
results consult persons who do have that knowledge, for example, application
experts of Leica Biosystems.
6.2.2 Universal cassette clamp 1
2 Fig. 49
Leica Biosystems cassettes with minimum dimensions of 39.8 x 28 mm and maximum
dimensions of 40.9 x 28 mm may be clamped in the universal cassette clamp
(UCC) horizontally as well as vertically.
· Pull the lever ( “Fig. 49-1”) towards the operator.
· Mount the cassette ( “Fig. 49-2”) horizontally or vertically as required.
· To clamp the cassette, release the lever ( “Fig. 49-1”).
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Optional Accessories 6
Warning
Insufficient frame conditions for sectioning. Specimen damage or poor
sectioning results, for example, sections alternating in thickness,
compressed, folded or stripped sections.
· Do not continue sectioning if you recognize insufficient sections results. ·
Make sure that all prerequisites for appropriate sectioning are met. Refer to
the troubleshooting
section of these Instructions for Use for further details. · If you do not
have sufficient knowledge in regards of troubleshooting insufficient
sectioning
results consult persons who do have that knowledge, for example, application
experts of Leica Biosystems.
Warning
The remaining broken edge of cassette lid may lead to poor section quality due
to insecure clamping. Serious injury of persons.
· When using a cassette whose lid is molded on, make sure that the broken edge
left by removing the lid does not prevent the specimen from being securely
clamped if necessary, the specimen must be clamped horizontally.
Caution
When using thin-walled cassettes, the cassettes become deformed or insecurely
clamped into place, or other problems because of the clamping system. Damage
to specimen/Delayed diagnosis.
· Be careful when using thin-walled-cassettes. Make sure that the thin-walled-
cassettes are clamped securely in place.
· If the user tries clamping the cassette and realizes that it is not securely
clamped into place, use a more stable one.
Caution
Paraffin deposits on the outside of the cassette can make the universal
cassette clamp dirty. The dirt prevents the cassette from clamping in securely
and can lead to sections being too thick or thin, chattering within the
section and, in the worst-case scenario, damage to the specimen.
· Prior to sectioning, the user has to verify that the specimen is clamped
securely. · Remove paraffin deposits from the universal cassette clamp.
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6 Optional Accessories
6.2.3 Super cassette clamp
2
3
4
5
1 Fig. 50
Assembly of the super cassette clamp
Tips Remove the rubber ring ( “Fig. 50-5”) only after having attached the
rigid fixture for specimen clamps on the object head.
The super cassette clamp should preferably be used with the rigid fixture for
specimen clamps. To do so, proceed as follows: · Screw the rigid fixture for
specimen clamps ( “Fig. 50-4”) onto the object head ( “Fig. 50-3”):
Remove the screw ( “Fig. 50-1”), place the rigid fixture for specimen clamps (
“Fig. 50-4”) onto the object head ( “Fig. 50-3”) from the front and tighten
the screws ( “Fig. 50-2”) with a Hexagon key No. 3. Next, insert the screw (
“Fig. 50-1”) from the side and briefly tighten it with a Hexagon key No. 4. ·
Insert the super cassette clamp from the side on the left into the dovetail
guide of the rigid fixture for specimen clamps and tighten screw ( “Fig.
50-1”). Caution When using super cassette clamp, the orientation is not set to
position “0” when directional fixture for specimen clamps is used with the
rigid knife holder base, or the back-lighting illumination is attached. The
instrument may malfunction leading to delayed diagnosis. · The orientation
must be in position “0” and the cover for backlighting illumination must be
detached. · NEVER use the super cassette clamp with backlighting illumination.
· The force balance system has to be adjusted when using the super cassette
clamp.
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Version 1.7, Revision J
6.3 Knife holder base and knife holder
1 Fig. 51 6.3.1 Two-in-one blade holder E 1 2
Optional Accessories 6
The plastic handles of all clamping levers on the instrument and knife holders
can be turned to the position that is most convenient for each user. Pull the
grip ( “Fig. 51-1”) out of the lever, hold it in this position, and rotate it
to the desired position. It will then lock automatically when released.
6
7
3 8
4
9
5
Fig. 52
1 Blade ejector
6 Safety guard
2 Clamping top 3 Clamping lever (left) 4 Segment arc 5 Blade holder base
7 Bolt 8 Clamping lever (right) 9 Pressure plate
The two-in-one blade holder optimized for use with Leica Biosystems disposable blades: low-profile blades (L x H x W): (80 +/-0.05) mm x (8 +0/- 0.1) mm x (0.254 +/-0.008) mm; high-profile blades (L x H x W): (80 +/-0.05) mm x (14 +0/-0.15) mm x (0.317 +/-0.005) mm. The two-in-one blade holder E has a lateral displacement function, so that the entire width of the blade can be used. It is equipped with a blade ejector to safely remove the blade after use. The pressure plate is replaceable.
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6 Optional Accessories
Tips
The clamping levers on the two-in-one blade holder E are not interchangeable.
The two clamping levers ( “Fig. 52-8”) ( “Fig. 52-3”) must remain in the
position shown at all times, as otherwise isolated malfunctions of the two-in-
one blade holder E can occur.
Clamping lever for the blade ( “Fig. 52-8”) is at the right, clamping lever
for the lateral displacement ( “Fig. 52-3”) is at the left.
1 Fig. 53
Lateral displacement
The lateral movement feature of the two-in-one
blade holder E enables the use of the entire length
of the blade by moving the clamping top laterally.
Three predefined stop positions (left, center,
right) that correspond to the width of a standard
2
cassette may be used if desired.
· To release the clamping, rotate the lever
( “Fig. 53-1”) on the left side of the two-in-one blade holder E
counterclockwise.
· Move the clamping-top ( “Fig. 53-2”) laterally.
· To clamp, rotate the lever ( “Fig. 53-1”) clockwise.
Warning
Too thick or thin blade used in the two-in-one blade holder. Sample impaired.
· Use a blade with a maximum thickness of less than 0.322mm and a minimum
thickness of more than 0.246mm.
· The recommended compatible blade is listed in Optional Accessories ( P. 61
6. Optional Accessories).
Warning
The blade is not installed completely parallel to the upper edge of the
pressure plate due to excess paraffin, uncleaned inserts, etc.. When using the
lateral movement function, failure to install the blade parallel to the
pressure plate may result in poor sectioning results. For example, if a
section is too thick or thin, chattering within the section, in the worst-case
scenario, may damage the specimen.
· Do not continue the sectioning when getting unsatisfying section results. ·
Re-install the blade and make sure that it is parallel to the upper edge of
the pressure plate. · Always check the parallelism between the blade and
pressure plate after moving the blade using
the lateral movement function. · Ensure there is no paraffin waste and the
insert is clean before use.
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Version 1.7, Revision J
Optional Accessories 6
6.3.2 Knife holder base, without lateral displacement feature
Repositioning the knife holder base The one-piece knife holder base (rigid) ( “Fig. 54-2”) can be moved forwards and backwards on the microtome base plate. This vertical displacement allows bringing the knife holder into the optimal cutting position in relation to the specimen.
· To release, rotate the clamping lever ( “Fig. 54-1”) on the right side of
the microtome base plate counterclockwise.
2 · Reposition the knife holder together with the knife holder base forward or
backward as appropriate.
Fig. 54
1
· Secure the clamping mechanism by rotating the lever ( “Fig. 54-1”) clockwise.
6.3.3 Knife holder E with a water trough for low-profile blades
3 Fig. 55
1 2
The knife holder E with a water trough ( “Fig. 55”) is only for low-profile
blades with the blade
dimensions (L x H x W):
(80 +/-0.05) mm x (8 +0/- 0.1) mm x (0.254 +/-0.008)
mm.
The safety guard on knife holder E consists of a
red foldaway handle ( “Fig. 55-1”). To cover the cutting edge, fold the safety
guard handle ( “Fig. 55-1”) clockwise as illustrated in the figure.
Tips
The clamping levers on the knife holder are not interchangeable. The two
clamping levers (( “Fig. 55-2”) and ( “Fig. 55-3”)) must remain in the
position shown at all times, as otherwise isolated malfunctions of the knife
holder can occur.
Clamping lever for the blade ( “Fig. 55-2”) is at the right, clamping lever
for the lateral displacement ( “Fig. 55-3”) is at the left.
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6 Optional Accessories
Fig. 56
Use Floating thin paraffin sections (for example, for subsequent immunostaining procedures) on the surface of the water. The vessel is filled with distilled or deionized water up to the blade. After trimming, remove the section waste from the vessel and create the sections to be prepared. The sections floating on the surface of the water can be collected using the glass slide.
6.3.4 Knife holder N
The knife holder N is appropriate for standard steel knives, profile c and d,
up to 16 cm long. The integrated height adjustment feature allows you to also
use knives that have been resharpened numerous times. · Knife holder N ( “Fig.
57”): For holding conventional knives up to 16 cm in length.
6
1
5
4 Fig. 57
2 3
Mounting the knife support bar
· Push the safety guard ( “Fig. 57-1”) to the center. · Set the knife support
bar ( “Fig. 57-2”) onto the height adjustment screws. The flat ends of the
height
adjustment screws must be located in the slots at each end of the knife
support bar.
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Optional Accessories 6
Warning
The knife/blade is installed before the knife/blade holder and the knife/blade
holder base are installed on the instrument. Serious injury of persons due to
contact with extremely sharp knives and/or blades.
· Prior to inserting the knife/blade, both the knife/blade holder and the
knife/blade holder base must be installed on the microtome.
Inserting the knife
· Rotate the knurled nuts ( “Fig. 57-3”) on the right and left of the knife
holder forward in opposite directions, lowering the knife support bar to the
lowest possible position, thus ensuring that the knife
edge will not be damaged when inserting the knife.
· Unscrew the clamping screws ( “Fig. 57-4”) (rotate counterclockwise) until
the knife can safely be inserted.
· Hold the knife ( “Fig. 57-5”) at the knife base and carefully insert it in
the holder from the side as shown with the cutting edge facing upward.
1
2
Fig. 58
Either side of the profile c knife ( “Fig. 58-1”) can be inserted, whereas the
facet of profile d knife ( “Fig. 58-2”) should face the operator. Putting the
knife wrongly will damage the specimen and the knife.
Knife height adjustment
When adjusting the knife height, the knife edge should be positioned as
exactly as possible in the actual center of rotation of the knife holder. The
lay-on edge ( “Fig. 57-6”) of the rear clamping chucks serves as a reference
position for correct knife height adjustment. · Rotate the knurled nuts (
“Fig. 57-3”) uniformly and backwards until the knife blade is parallel to the
marks on the lay-on edges ( “Fig. 57-6”) of the rear clamping chucks. · To
clamp the knife ( “Fig. 57-5”) evenly screw the two knife clamping screws (
“Fig. 57-4”) inward
(rotate clockwise).
Lateral repositioning of the knife
· Push the safety guard ( “Fig. 57-1”) to the center. · Loosen the clamping
screws ( “Fig. 57-4”) by turning them counterclockwise. · Push the knife (
“Fig. 57-5”) to the left or right as required. · After the knife has been
repositioned, adjust the knife height ( P. 71 Knife height adjustment),
then tighten the clamping screw ( “Fig. 57-4”) which is located on the side to
by turning it clockwise to clamp the knife ( “Fig. 57-5”).
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Caution After the knife/blade holder is laterally displaced, the specimen is
not retracted and retrimmed. Specimen damage.
· Retract the object head and retrim the specimen block each time after
laterally displacing the knife/blade holder.
6.4 Section waste tray 2
1 3
The anti-static section waste tray offers ease of
cleaning due to the anti-static surface.
Push the section waste tray ( “Fig. 59-1”) from the front to the microtome
base plate ( “Fig. 59-2”) until it is held in place by the two magnets
( “Fig. 59-3”) (on the front of the microtome base plate).
Fig. 59
6.5 Backlighting Tips The backlighting cannot be used with the blade holder
base of the two-in-one blade holder E. To use with backlighting on HistoCore
MULTICUT, the External power supply unit Backlighting (Order No.: 14 0500
31244 ) must be ordered separately.
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Version 1.7, Revision J
Optional Accessories 6
1 2
3
5 4
· Remove the two screws ( “Fig. 60-1”) using a slotted screwdriver and then
remove the cover plate ( “Fig. 60-2”).
· Insert the backlighting ( “Fig. 60-3”) in the recess at the rear of the
knife holder base.
· Connect the plug ( “Fig. 60-4”) of the backlighting to the plug of the
External power supply unit Backlighting ( “Fig. 60-5”).
· Select a proper plug which is in correct voltage and connect it with the
adapter. Insert the plug into the power socket and the backlighting will be
illuminated.
Fig. 60
Caution
When using super cassette clamp, the orientation is not set to position “0”
when directional fixture for specimen clamps is used with the rigid knife
holder base, or the back-lighting illumination is attached. The instrument may
malfunction leading to delayed diagnosis.
· The orientation must be in position “0” and the cover for backlighting
illumination must be detached. · NEVER use the super cassette clamp with
backlighting illumination. · The force balance system has to be adjusted when
using the super cassette clamp.
6.6 Top tray
The top tray is mounted on the hood of the microtome. The mark ( “Fig. 61-1”)
on both the top tray and the hood helps to orient the installation of the top
tray. The top tray is for storage of the utensils used during sectioning as
well as the sectioned specimens.
1
Fig. 61
6.7 Universal microscope carrier Tips Unpack all accessories in the package and check for completeness.
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6 Optional Accessories
11
1
Fig. 62
14 15
8
2
7
3
6
13 12 Fig. 63
4 5
· ( “Fig. 62-1”), Baseplate with drilling ( “Fig. 62-11”)
· ( “Fig. 63-2”), Vertical column with size 8 cap screw ( “Fig. 63-12”) and
lock washer ( “Fig. 63-13”)
· ( “Fig. 63-3”), Horizontal arm with crossmember ( “Fig. 63-14”) and support
ring ( “Fig. 63-15”)
· ( “Fig. 63-4”), Support plate, large (for BIOCUT, MULTICUT and AUTOCUT)
· ( “Fig. 63-5”), Support plate, small (for NANOCUT R)
· ( “Fig. 63-6”), Hexagon key No. 3
· ( “Fig. 63-7”), 4 countersunk screws to install support plate
· ( “Fig. 63-8”), Hexagon key size 8
Fig. 64
Assembling the universal microscope carrier
· Attach baseplate. Select the large ( “Fig. 63-5”)or small ( “Fig. 63-4”)
support plate, depending on the microtome to be used. Attach the support plate
to the baseplate with the included 4 countersunk screws ( “Fig. 63-7”) using
the Hexagon key No. 3 ( “Fig. 63-6”).
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Version 1.7, Revision J
12 13
Fig. 65
2
Optional Accessories 6
· Attach vertical column. Insert the cap screw ( “Fig. 65-12”) into the hole
of the baseplate from below. Place the lock washer ( “Fig. 65-13”) on the cap
screw from above. Thread the silver vertical column ( “Fig. 66-2”) onto the
baseplate from above and tighten with the Hexagon key size. 8.
19
Fig. 66
Warning The universal microscope carrier tips. Serious injury of persons.
· After installing the vertical column, immediately place the microtome onto
the baseplate so that the front feet of the microtome are positioned in the
shallow recesses ( “Fig. 66-19”).
Tips The lock washer must be positioned between the baseplate and vertical
column to prevent unintentional turning of the column.
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6 Optional Accessories
2 18 3
16
14 17
15
· Attach horizontal arm. Slide the support ring
( “Fig. 67-15”) onto the vertical column and position it so that the lock nut
( “Fig. 67-16”) is facing to the back. Tighten the lock nut. Slide
the cross-member ( “Fig. 67-14”) onto the column. Ensure that the lock screw (
“Fig. 67-17”) is facing to the right of the baseplate.
The horizontal arm must be centered over the
microscope. Slide the horizontal arm ( “Fig. 67-3”), flat side facing the lock
screw ( “Fig. 67-17”), into the cross-member ( “Fig. 67-14”) and tighten.
Fig. 67
Tips
For more information on connecting and using the microscope, magnifying lens
or cold-light source, please see the appropriate Instructions for Use.
6.8 Magnifying lens, LED illumination
Tips The magnifying lens provides a 2x magnification and can be used with all
HistoCore series rotary microtomes.
1
2
3 4
Fig. 68
· Open the screw ( “Fig. 68-3”) on the horizontal arm of the microscope
carrier in a counterclockwise direction.
· Insert the silver connection piece ( “Fig. 68-1”) as far as it will go.
Tighten the screw ( “Fig. 68-3”). · The adapter ( “Fig. 68-2”) allows the LED
Hi-power spots to be installed. Connect the LED 1000 Hi-
Power spots, 2-arms, with the adapter ( “Fig. 69”). Insert the plugs ( “Fig.
69-1”) of the LED 1000 Hi-Power spots, 2-arms, into the sockets ( “Fig. 69-2”)
of the high-power spot controller ( “Fig. 69-3”). Connect the power adapter (
“Fig. 69-4”) of the LED 1000 control unit to the high-power spot
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Version 1.7, Revision J
Optional Accessories 6
controller, then connect to the power supply. Make sure to choose a correct
plug from the provided ones ( “Fig. 69-5”) for the power adapter for your
region. · Press the button ( “Fig. 69-7”) on the high-power spot controller to
turn on or turn off the two spot lights ( “Fig. 69-6”), which is indicated
correspondingly by two green lights ( “Fig. 69-8”) on the high-power spot
controller. Turn the control wheel ( “Fig. 69-9”) to adjust the brightness of
the spot lights.
8
1
9
7
6
3
2
4
5
Fig. 69
· Adjust the position of the magnifying lens to the specimen being processed.
The magnifying lens can be swiveled completely to the side if necessary.
Warning
Magnifying lens is not covered when not working with it. Serious injury of
persons and/or property damage due to burning glass effect. Magnifying lens
can set surrounding objects on fire, especially when there is exposure to
direct sunlight.
· Always cover the magnifier lens when not working with it. · Especially
protect it from exposure to direct sunlight.
· Use the provided protective cap ( “Fig. 68-4”) to cover the magnifying lens.
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6 Optional Accessories
6.9 Additional accessories Knife holder base, non-orientable
Silver for knife holder N and knife holder E with trough
· Order No.: 14 0502 37962
Fig. 70
Knife holder N
Fig. 71
Knife holder E with trough
Fig. 72
Silver, for holding conventional knives up to 16 cm in length Height
adjustment of the knife Separate clearance angle adjustment Movable safety
guard
· Order No.: 14 0502 37993
For low-profile disposable blades, dimensions (L x H x W): (80 +/-0.05) mm x
(8 +0/- 0.1) mm x (0.254 +/-0.008) mm, silver, with a water trough Quick clamp
system with ability to shift the clamping top laterally 3 click-stop positions
make it easier to utilize the entire blade length Color-coded, foldaway safety
guard
· Order No.: 14 0502 38961
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Version 1.7, Revision J
Blade holder base for two-in-one blade holder E
Optional Accessories 6
Blade holder base for two-in-one blade holder E · Order No.: 14 0502 55546
Fig. 73
Two-in-one blade holder E
Fig. 74
Disposable blades — low profile (Leica 819)
For high and low profile disposable blades with the blade dimensions for low- profile blades (L x H x W): (80 +/-0.05) mm x (8 +0/-0.1) mm x (0.254 +/-0.008) mm, and the blade dimensions f
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