OHAUS ST400D-G Optical Dissolved Oxygen Meter Instruction Manual

June 12, 2024
Ohaus

OHAUS ST400D-G Optical Dissolved Oxygen Meter

Product Information

Item Description
Model STARTER 400D
Type Portable Optical DO (Dissolved Oxygen) Meter
Manufacturer OHAUS

Product Usage Instructions

Installation

Before using the STARTER 400D Portable DO Meter, follow the steps below for installation:

  1. Check the package contents and ensure all items are included.
  2. Install the batteries according to the instructions provided.
  3. Attach the electrode clip as per the provided guidelines.
  4. Install the IP54 cover caps to protect the device.
  5. If required, use the integrated stand for table top use.

Setup

No specific setup instructions are mentioned in the user manual.

Operation

Calibration

To calibrate the STARTER 400D Portable DO Meter, follow the steps below:

  1. Prepare a zero-oxygen solution.
  2. Perform a 1-point calibration using the provided guidelines.
  3. Perform a 2-point calibration as per the instructions provided.

Sample Measurement

To measure samples using the STARTER 400D Portable DO Meter, follow the instructions provided in the user manual.

**Temperature Measurement

** To measure temperature using the STARTER 400D Portable DO Meter, follow the instructions provided in the user manual.

Barometer Pressure Measurement
To measure barometer pressure using the STARTER 400D Portable DO Meter, follow the instructions provided in the user manual.

Using the Memory
The STARTER 400D Portable DO Meter has a memory function. Follow the instructions provided in the user manual to store, recall, and clear readings from the memory.

Maintenance
Regular maintenance is required for optimal performance of the STARTER 400D Portable DO Meter. Refer to the user manual for instructions on handling error messages, meter maintenance, and probe maintenance.

Technical Data

The technical specifications of the STARTER 400D Portable DO Meter can be found in the user manual.

INTRODUCTION
Thank you for choosing OHAUS. Please read the manual completely before using the STARTER 400D portable Optical DO (Dissolved Oxygen) meter to avoid incorrect operation. The STARTER 400D has an excellent performance/price ratio and is designed with many useful features. Optical DO probe Other accessories are also available from OHAUS.

Starter 400D offers many practical features such as:

  • Ergonomic, multifunctional design for ambidextrous measurements or bench top use
  • Meter can measure barometric pressure automatically to compensate the DO value; Salinity value can be set to compensate the DO value.
  • IP54 protection against dust and water
  • Ohaus’ renowned user friendly software

Definition of Signal Warnings and Symbols
Safety notes are marked with signal words and warning symbols. These show safety issues and warnings. Ignoring the safety notes may lead to personal injury, damage to the instrument, malfunctions and false results

Signal Words

  • WARNING For a hazardous situation with medium risk, possibly resulting in injuries or death if not avoided.
  • CAUTION For a hazardous situation with low risk, resulting in damage to the device or the property or in loss of data, or injuries if not avoided.
  • Attention For important information about the product
  • Note For useful information about the product

Warning Symbols

Safety Precautions
CAUTION Read all safety warnings before installing, making connections, or servicing this equipment. Failure to comply with these warnings could result in personal injury and/or property damage. Retain all instructions for future reference.

  • Use the equipment only in dry locations.
  • Dry off any liquid spills immediately. The instrument is not watertight.
  • Use only approved accessories and peripherals.
  • Operate the equipment only under ambient conditions specified in these instructions.
  • Do not operate the equipment in hazardous or unstable environments.
  • Service should only be performed by authorized personnel.
  • If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

WARNING Do not use this instrument in an environment subject to explosion hazards. The housing of the instrument is not gas tight (explosion hazard due to spark formation, corrosion caused by the ingress of gases).
CAUTION When using chemicals and solvents, comply with the instructions of the producer and the general lab safety rules.

Display and controls

Display

1 Time: HH:MM:SS
2 Date: YYYY-MM-DD
3 ****

calibration data: |K-1|≤0.3 and |B| <0.1 Probe condition is very good

| ****

calibration data: 0.3≤|K-1|≤0.4 and |B| <0.1 Probe condition is acceptable.

4| Measurement icon – measurement (calibration) is running
5| Calibration icon – calibration is running
6| Power state icon, include power always on icon , means meter will not automatically turn off; and auto-off icon which means after 10 minutes no operation meter will turn off (see 4.4); power state icon position is at the top-left of the battery status icon.
7| Battery status icon – the charged status of the battery
8| DO reading: mg/L and %
9| Temperature during measurement or in calibration process
10| Barometric pressure during measurement or in calibration process
11| Data log: memory number
12| ppt for salinity entry

Controls

INSTALLATION

Carefully unpack the box.

Package contents
The ST400D kit has the following items:

ST400D Units
STARTER 400D meter 1
STDO21 (with 1.0m cable) 1
AAA battery 4
Electrode Clip 1
IP54 cover caps 1 set
Wrist Strap 1

We also offer ST400D-G kit which includes ST400D kit and a portable bag.

Accessories

Model Description P/N
STDO21 Optical DO probe 30219117

3020

DO Zero Oxygen Chemical 30059257
Portable Bag for portable meters 30031635

Installing the batteries

  • Remove the battery cover screw using a coin or screwdriver and remove the battery cover
  • Insert the batteries in the battery compartment as shown
  • Replace the battery cover and tighten the battery cover screw

Installing the e lectrode clip

The electrode clip is an electrode holder that can be placed next to the display on either side of the housing.

  • Attach the clip by inserting the tabs into the recess.
  • Slide the clip forward to lock in position. Slide the shaft of the probe into the clip from above.

Installing the IP54 cover caps
Use the small plastic tool as shown:

Integrated stand for table top use
Starter 400D has an integrated table stand as shown, which can be pushed out to use the meter on a lab bench or desk. To close the stand, push the middle edge of the stand toward the meter

SETUP

Press to enter the setup interface. We have 7 setting items, they are:

  1. Auto/Manual Endpoint
  2. Salinity Value
  3. Temperature Unit
  4. Date and Time
  5. Power State
  6. Reset Factory Setting
  7. New Probe Cap Parameter

Setting Auto/Manual Endpoint

When in calibration, ST400D is only work at Auto End Point Mode.
The default endpoint mode of ST400D for measurement is Auto End Point Mode. When users want to judge the endpoint manually, you can shift to Manual Endpoint Mode.

End point Mode:

  • When in Manual Mode, to manually reach and lock a DO measurement, you need to press button- when reading is stable then the measurement value freezes; And meter displays .
  • When in Auto End Point Mode, the meter determines when the reading is stable then locks the measurement or calibration value automatically, the reading freezes; the meter displays .

Users can also change the endpoint mode by shortcut — Press and hold button- to shift the End Point Mode.

Setting Salinity Value
Since salinity has an influence on the dissolved oxygen value, it is important to input the correct salinity value. The meter can compensate salinity for measurements in solutions with salinity from 0 to 50 ppt. The default value for salinity is 0.0 ppt

  • Press button- or button- to adjust the salinity value.
  • Press button- to confirm the value or press button- to return to the Setting screen.

Normally seawater salinity is 35ppt, which means 35g of salt per 1000g seawater.

Setting Temperature Unit

Ohaus optical DO probe STDO21 include a built-in temperature probe, the meter will be in Automatic Temperature Compensation (ATC) mode automatically. You can select the temperature unit according to your preferred: °C or °F.
Note : °C = 5/9 (°F -32)
The default setting is 25 °C (77°F).

Setting the Date and Time
The Date format is yy-mm-dd. Press button- or button- to adjust the value of each numerical digit. Press button- to confirm current value then goes to next numerical digit.

Setting Power State

  • We have 2 power states: “Auto OFF” or “Always ON”.
    When “Auto OFF” is selected, which means the meter will turn off the powder without any press operation after 10 minutes and auto-off icon will display at the top-left of the battery status icon .

  • “Always ON” means power will not turn off automatically, and the power always-on icon displays.

  • Ohaus suggest you to select “Auto OFF” so to save power of batteries.

Setting Factory Reset

Press button- and button- together to enter the Reset Factory Setting screen.

Setting New Probe Cap Parameter
When replace a new probe cap, user must input the parameter of this new cap into the meter. Or the measurement value will be incorrect

Press button- , button- and button- together to enter the new probe cap parameter setting screen. Input the parameter of the new cap. Then do calibration before any measurement.

OPERATION

  • WARNING Do not operate the equipment in hazardous environments. The equipment is not explosion protected.
  • WARNING When using chemicals and solvents, comply with the instructions of the chemical producer and the general lab safety rules.

Calibration

STARTER 400D allows you to perform 1-point or 2-point calibrations; Ohaus suggest 1-point calibration(100% DO calibration) for samples which DO value is not less than 2 mg/L.
Only when measure samples which DO value is lower than 2 mg/L, Ohaus suggest 2-point calibration.

Zero-oxygen solution
Preparing a zero-oxygen solution: put approximately 20ml of pure or deionized water into the bottle of DO Zero Oxygen Chemical (30059257, there are about 5g powder in it), stir for at least 5 minutes.
This zero-oxygen solution is used for 2-point calibration

Performing 1-point calibration
When performing calibration, the meter only uses Auto End Point Mode. 1 point calibration can be performed in the air or oxygen-saturated water. There will be a little difference between calibration in the air and in oxygen-saturated water. Before calibration you need to rinse the DO probe with pure water and carefully wipe the water off with a tissue.

  • Place the probe in the air (or in oxygen-saturated water).
  • Press button- , the calibration icon appear on the display.
  • The meter reaches endpoints automatically, 100% will display. Measurement icon disappears.

Once you have completed your 1-point calibration, you have 3 options new:

  • Press button- to finish 1-point calibration then return to measurement screen.
  • Press button- to reject calibration and return to measurement screen.
  • Press button- to complete a 2-point calibration (after preparing the zero oxygen solution and putting STDO21 into it).

Performing 2-point calibration

  • Perform the 1-point calibration.
  • Place the probe in the zero oxygen solution and press button- .

The meter reaches the endpoint automatically. 0% will display then return to measurement screen. Pressing and holding button- will display the calibration data.

Sample measurement

  • Dissolved oxygen readings can display unit of mg/L or %.
  • Place the DO probe in the sample. Press to start the measurement.
  • When the meter reaches endpoint, the measurement reading freezes on the screen

Temperature measurement

For better accuracy, we recommend the use of the temperature probe. If a temperature probe is used, ATC and the sample temperature are displayed.
STDO21 has a built-in temperature probe.

Barometer pressure measurement

Barometric pressure influences the DO value.
STARTER 400D has a built-in barometric pressure sensor which can measure the barometric pressure automatically. This meter uses barometric pressure unit KPa (kilopascal).

Using the memory

Storing a reading

The STARTER 400D can store up to 99 measurement readings. Press button- when the measurement reaches end point. indicates that is the 2nd measurement reading had been stored. The starter 400D can store up to 99 measurement readings

Recalling from memory

  • Press and hold button- to recall the stored values from memory.
  • Press button- or button- to scroll through the stored results. Press button- to go back to the measurement screen.

Clearing the memory
Continue pressing button- to scroll through the stored results until “Clear All” appears.
Press button- to confirm the deletion or press button- to return to the measurement mode without deleting all the data in the memory.

MAINTENANCE

Error message

Low battery| If low battery, the battery icon will blink to show need new battery. Then turn off after 10 seconds.
---|---
Communication fail| When do calibration or new cap parameter input, meter will detect if the probe is connected properly. If not, meter displays ” Fail, please confirm the STDO21 probe is connected”
Measured temperature out of range (< -5°C or >55 °C)| Meter will displays “Temperature Out of Range!”

Please keep the sample temperature within the range for measurement.

Calibration out of range| Meter displays “Calibration Fail”.

|K-1| >0.4 or a |B| >0.1

You may need to do a proper calibration again, if calibration data is still not good, you may need to replace a new probe cap or probe.

Recall memory fail| Meter displays “No Data”

When recall memory while no data memorized yet.

The current data set has already been stored once| Meter displays” Already saved!”

A measurement can only be stored once. Perform a new measurement to store a new data set.

Any more problems, you can contact Ohaus.

Meter maintenance

  • The STARTER 400D does not require any maintenance other than replacement of depleted batteries. To clean, use a damp cloth.
  • Attention : Do not disassemble the instrument. There are no user serviceable parts inside.
  • Attention : The housing is made of acrylonitrile butadiene styrene (ABS). This material is susceptible to damage by some organic solvents, such as toluene, xylene and methyl ethyl ketone (MEK). Any spillage should be immediately wiped off

Probe maintenance

  • The DO probe measures the oxygen based on luminescence principle. The optical DO probe measures dissolved oxygen by emitting a blue light of the proper wavelength that causes the sensing element to luminesce. Oxygen dissolved in the sample continually passes through the diffusion layer to the sensing layer, affecting the luminescence lifetime. STDO21 can measure the lifetime of the luminescence as it is affected by the presence of oxygen and compared with the reference. This technology has been approved by EPA.

  • When measurement, optical probe did not consume the DO in the sample, that means the sample do not need stir
    Optical probe STDO21 do not need to replace the membrane or any maintenance of anode or cathode. The optical probe only requires that its sensor cap be replaced every 12-18 months. Please input the new cap parameter when new probe cap in installed, then do calibration before any measurement.

  • When storing the probe for a longer period, make sure it is placed in the wetting protection cover.

  • Calibration frequency: Ohaus suggest verifying the calibration regularly according to your measurement requirement. STDO21 only has very little calibration drift when compared to electrochemical probes. This advantage results in less frequent calibrations. In fact, optical probes are so stable they are capable of holding their calibration for many months.

  • Please also read the STDO21 probe manuals before using it.

TECHNICAL DATA

Specifications

Ambient conditions

  • Altitude: Up to 2000 m
  • Specified Temperature range: 5°C to 40°C
  • Humidity: maximum relative humidity 80 % for temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C
  • Installation category: N/A
  • Pollution degree: N/A
  • Operability is assured at ambient temperatures between 5°C to 40°C

Model : ST400D

Measurement Range

| %| 0.0 to 200.0%
---|---|---
mg/L| 0.00 to 20.00mg/L (ppm)
Temp.| 0 to 50 °C

Measurement Resolution

| %| 0.1%
mg/L| 0.01 mg/L
Temp.| 0.1 °C

Accuracy

| ± 0.2 mg/L (<8 mg/L)

± 0.3 mg/L (8 to 20 mg/L);

± 0.3 °C

Barometric

| Range| 50.0 to 115.0 kPa
Resolution| 0.1 kPa
Accuracy| 1.5 kPa
Temp compensation| ATC
Salinity compensation| 0.0 to 40.0 ppt
Calibration| 1 point or 2 points
Memory| 99 sets, last calibration data
Power| 4 x AAA(LR03) batteries
Size/Weight| 90 W x 150 D x 35 H mm
Weight| 0.16kg
Display| liquid crystal
IP rating| IP54
T input| 4-pin screw
Materials| ABS

Compliance

Compliance to the following standards is indicated by the corresponding mark on the product.

Standard

This product complies with the applicable harmonized standards of EU Directives

2011/65/EU (RoHS) and 2014/30/EU (EMC). The EU Declaration of Conformity is available online at www.ohaus.com/ce.

This product complies with the EU Directive 2012/19/EU (WEEE). Please dispose of this product in accordance with local regulations at the collecting point specified for electrical and electronic equipment.

For disposal instructions in Europe, refer to www.ohaus.com/weee.

EN 61326-1

ISED Canada Compliance Statement
This Class A digital apparatus complies with Canadian ICES-003.

ISO 9001 Registration
The management system governing the production of this product is ISO 9001 certified.

LIMITED WARRANTY

  • Ohaus products are warranted against defects in materials and workmanship from the date of delivery through the duration of the warranty period. During the warranty period Ohaus will repair, or, at its option, replace any component(s) that proves to be defective at no charge, provided that the product is returned, freight prepaid, to Ohaus.
  • This warranty does not apply if the product has been damaged by accident or misuse, exposed to radioactive or corrosive materials, has foreign material penetrating to the inside of the product, or as a result of service or modification by other than Ohaus. In lieu of a properly returned warranty registration card, the warranty period shall begin on the date of shipment to the authorized dealer. No other express or implied warranty is given by Ohaus Corporation. Ohaus Corporation shall not be liable for any consequential damages.
  • As warranty legislation differs from state to state and country to country, please contact Ohaus or your local Ohaus dealer for further details

Ohaus Corporation 7 Campus Drive Suite 310
Parsippany, NJ 07054 USA
Tel: +1 973-377-9000
Fax: +1 973-944-7177
With offices worldwide
www.ohaus.com
PN 30208731D ©2019 Ohaus Corporation, all rights reserved

References

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